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- PDB-8daf: Human SF-1 LBD bound to synthetic agonist 6N-10CA and bacterial p... -

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Basic information

Entry
Database: PDB / ID: 8daf
TitleHuman SF-1 LBD bound to synthetic agonist 6N-10CA and bacterial phospholipid
Components
  • Nuclear receptor coactivator 2
  • Steroidogenic factor 1
KeywordsNUCLEAR PROTEIN / SF-1 / Nuclear Receptor / Ligand / Synthetic Agonist / Steroidogenic factor-1 / NR5A1
Function / homology
Function and homology information


primary sex determination / response to gonadotropin-releasing hormone / Sertoli cell differentiation / negative regulation of female gonad development / sex determination / regulation of steroid biosynthetic process / positive regulation of male gonad development / Transcriptional regulation of testis differentiation / luteinization / calcineurin-mediated signaling ...primary sex determination / response to gonadotropin-releasing hormone / Sertoli cell differentiation / negative regulation of female gonad development / sex determination / regulation of steroid biosynthetic process / positive regulation of male gonad development / Transcriptional regulation of testis differentiation / luteinization / calcineurin-mediated signaling / Transcriptional regulation of pluripotent stem cells / tissue development / Leydig cell differentiation / male sex determination / maintenance of protein location in nucleus / hormone metabolic process / adrenal gland development / RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding / female gonad development / locomotor rhythm / aryl hydrocarbon receptor binding / regulation of lipid metabolic process / cellular response to Thyroglobulin triiodothyronine / regulation of glucose metabolic process / Synthesis of bile acids and bile salts / Endogenous sterols / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / regulation of cellular response to insulin stimulus / Recycling of bile acids and salts / cellular response to hormone stimulus / positive regulation of adipose tissue development / peroxisome proliferator activated receptor signaling pathway / RORA activates gene expression / Regulation of lipid metabolism by PPARalpha / hormone-mediated signaling pathway / BMAL1:CLOCK,NPAS2 activates circadian gene expression / Activation of gene expression by SREBF (SREBP) / SUMOylation of transcription cofactors / nuclear receptor coactivator activity / response to progesterone / transcription coregulator binding / nuclear receptor binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / phospholipid binding / circadian regulation of gene expression / Heme signaling / SUMOylation of intracellular receptors / mRNA transcription by RNA polymerase II / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Transcriptional activation of mitochondrial biogenesis / PPARA activates gene expression / Cytoprotection by HMOX1 / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / male gonad development / nuclear receptor activity / Circadian Clock / sequence-specific double-stranded DNA binding / HATs acetylate histones / Estrogen-dependent gene expression / transcription regulator complex / sequence-specific DNA binding / transcription by RNA polymerase II / transcription coactivator activity / nuclear body / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein domain specific binding / chromatin binding / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / enzyme binding / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / zinc ion binding / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
Nuclear hormone receptor family 5 / Nuclear receptor coactivator 2 / Nuclear receptor coactivator 2/3, DUF4927 / Domain of unknown function (DUF4927) / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator, Ncoa-type, interlocking / Nuclear receptor coactivator, Ncoa-type, interlocking domain superfamily / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator / DUF1518 ...Nuclear hormone receptor family 5 / Nuclear receptor coactivator 2 / Nuclear receptor coactivator 2/3, DUF4927 / Domain of unknown function (DUF4927) / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator, Ncoa-type, interlocking / Nuclear receptor coactivator, Ncoa-type, interlocking domain superfamily / Nuclear receptor coactivator, DUF1518 / Nuclear receptor coactivator / DUF1518 / Nuclear receptor coactivator, receptor-binding domain / Nuclear receptor coactivator / Steroid receptor coactivator / Unstructured region on nuclear receptor coactivator protein / PAS domain / Helix-loop-helix DNA-binding domain superfamily / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile. / Nuclear receptor coactivator, interlocking / PAS fold / PAS fold / PAS domain / PAS repeat profile. / PAS domain / PAS domain superfamily / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type
Similarity search - Domain/homology
Chem-IUW / DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / Steroidogenic factor 1 / Nuclear receptor coactivator 2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.59 Å
AuthorsD'Agostino, E.H. / Cato, M.L. / Ortlund, E.A.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)F31DK122745 United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01DK115213 United States
National Science Foundation (NSF, United States)DGE1444932 United States
Citation
Journal: J.Biol.Chem. / Year: 2023
Title: Comparison of activity, structure, and dynamics of SF-1 and LRH-1 complexed with small molecule modulators.
Authors: Cato, M.L. / D'Agostino, E.H. / Spurlin, R.M. / Flynn, A.R. / Cornelison, J.L. / Johnson, A.M. / Fujita, R.A. / Abraham, S.M. / Jui, N.T. / Ortlund, E.A.
#1: Journal: Acta Crystallogr D Biol Crystallogr / Year: 2010
Title: PHENIX: a comprehensive Python-based system for macromolecular structure solution.
Authors: Adams, P.D. / Afonine, P.V. / Bunkoczi, G. / Chen, V.B. / Davis, I.W. / Echols, N. / Headd, J.J. / Hung, L.W. / Kapral, G.J. / Grosse-Kunstleve, R.W. / McCoy, A.J. / Moriarty, N.W. / ...Authors: Adams, P.D. / Afonine, P.V. / Bunkoczi, G. / Chen, V.B. / Davis, I.W. / Echols, N. / Headd, J.J. / Hung, L.W. / Kapral, G.J. / Grosse-Kunstleve, R.W. / McCoy, A.J. / Moriarty, N.W. / Oeffner, R. / Read, R.J. / Richardson, D.C. / Richardson, J.S. / Terwilliger, T.C. / Zwart, P.H.
#2: Journal: Acta Crystallogr D Biol Crystallogr / Year: 2010
Title: Features and development of Coot.
Authors: Emsley, P. / Lohkamp, B. / Scott, W.G. / Cowtan, K.
#3: Journal: IUCrJ / Year: 2014
Title: The PDB_REDO server for macromolecular structure model optimization.
Authors: Joosten, R.P. / Long, F. / Murshudov, G.N. / Perrakis, A.
History
DepositionJun 13, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 28, 2023Provider: repository / Type: Initial release
Revision 1.1Aug 9, 2023Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.title
Revision 1.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Steroidogenic factor 1
B: Steroidogenic factor 1
C: Nuclear receptor coactivator 2
D: Nuclear receptor coactivator 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,1418
Polymers59,6554
Non-polymers2,4854
Water23413
1
A: Steroidogenic factor 1
C: Nuclear receptor coactivator 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)31,0704
Polymers29,8282
Non-polymers1,2432
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2080 Å2
ΔGint-19 kcal/mol
Surface area12210 Å2
MethodPISA
2
B: Steroidogenic factor 1
D: Nuclear receptor coactivator 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)31,0704
Polymers29,8282
Non-polymers1,2432
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2130 Å2
ΔGint-19 kcal/mol
Surface area12370 Å2
MethodPISA
Unit cell
Length a, b, c (Å)73.428, 73.428, 194.184
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number152
Space group name H-MP3121
Space group name HallP312"
Symmetry operation#1: x,y,z
#2: -y,x-y,z+1/3
#3: -x+y,-x,z+2/3
#4: x-y,-y,-z+2/3
#5: -x,-x+y,-z+1/3
#6: y,x,-z

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Components

#1: Protein Steroidogenic factor 1 / / SF-1 / STF-1 / hSF-1 / Adrenal 4-binding protein / Fushi tarazu factor homolog 1 / Nuclear receptor ...SF-1 / STF-1 / hSF-1 / Adrenal 4-binding protein / Fushi tarazu factor homolog 1 / Nuclear receptor subfamily 5 group A member 1 / Steroid hormone receptor Ad4BP


Mass: 28118.615 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NR5A1, AD4BP, FTZF1, SF1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q13285
#2: Protein/peptide Nuclear receptor coactivator 2 / / NCoA-2 / Class E basic helix-loop-helix protein 75 / bHLHe75 / Transcriptional intermediary factor 2 / hTIF2


Mass: 1708.931 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NCOA2, BHLHE75, SRC2, TIF2 / Production host: Homo sapiens (human) / References: UniProt: Q15596
#3: Chemical ChemComp-IUW / 10-[(3aR,6S,6aR)-3-phenyl-3a-(1-phenylethenyl)-6-(sulfamoylamino)-1,3a,4,5,6,6a-hexahydropentalen-2-yl]decanoic acid (non-preferred name)


Mass: 550.752 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C32H42N2O4S / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical ChemComp-PEF / DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / 3-[AMINOETHYLPHOSPHORYL]-[1,2-DI-PALMITOYL]-SN-GLYCEROL / Phosphatidylethanolamine


Mass: 691.959 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C37H74NO8P / Comment: phospholipid*YM
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.56 Å3/Da / Density % sol: 51.89 %
Crystal growTemperature: 277.15 K / Method: vapor diffusion / Details: Na acetate (pH 4.6), glycerol, PEG 4000

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Data collection

DiffractionMean temperature: 80 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 19, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.59→38.59 Å / Num. obs: 19623 / % possible obs: 99.8 % / Redundancy: 12.4 % / Biso Wilson estimate: 66.53 Å2 / Rpim(I) all: 0.035 / Net I/σ(I): 13.5
Reflection shellResolution: 2.59→2.68 Å / Num. unique obs: 1878 / Rpim(I) all: 0.453

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1ZDT

1zdt


Resolution: 2.59→38.59 Å / SU ML: 0.3698 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 31.4045 / Stereochemistry target values: CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2812 981 5 %
Rwork0.2226 18629 -
obs0.2255 19610 99.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 82.77 Å2
Refinement stepCycle: LAST / Resolution: 2.59→38.59 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3936 0 172 13 4121
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00574173
X-RAY DIFFRACTIONf_angle_d0.69595624
X-RAY DIFFRACTIONf_chiral_restr0.0375646
X-RAY DIFFRACTIONf_plane_restr0.0032700
X-RAY DIFFRACTIONf_dihedral_angle_d10.95412544
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.59-2.730.35031390.33692579X-RAY DIFFRACTION99.31
2.73-2.90.35221400.29432603X-RAY DIFFRACTION99.89
2.9-3.120.30681390.28272622X-RAY DIFFRACTION99.96
3.12-3.430.35181400.26372647X-RAY DIFFRACTION99.89
3.43-3.930.31861340.2272645X-RAY DIFFRACTION99.78
3.93-4.950.27711380.18972701X-RAY DIFFRACTION99.93
4.95-38.590.22381510.19682832X-RAY DIFFRACTION99.97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.47494989969-2.065002424582.008075667874.9226505729-0.8529300202332.01401449898-0.04739735013030.271505047213-0.443358689233-0.323810417143-0.417897404176-0.2542444671210.8194105210210.1589355879310.3463143796440.5559606994760.04521131165570.0456450651760.88694889997-0.04173829534570.648011797584-19.16505296119.162793347-4.8180526577
25.925812150570.358600198461-3.291376699968.28038395742-2.370904496016.891892485770.647330450979-1.2360277484-0.2314316997360.829331405685-0.9211631866230.6513761162990.253712956466-0.3591665939710.1479672948050.656105985827-0.1255921158770.0868523100461.09521517166-0.1823071621230.75454281677-23.296298785724.8454667811.0501107882
36.957924137821.510537897640.2644048761192.94354838251-0.0302013856336.07552580204-0.118847929265-0.860225857457-0.1595112442830.527394414051-0.02321751225130.5631825106230.121420558177-0.2371965256170.2268872453550.4746005467240.1462048457450.03836659809610.7189874296710.009252245407990.589909038946-10.395331596526.8550191891-3.42959305727
43.995118097680.9289223908390.6055602105287.19006379464-4.16158967559.07359488368-0.2726781282110.477760330803-0.2821557444340.093021660251-0.0997889413562-0.377614341825-0.1186444958440.783684353640.535705967010.5217719729440.02765452447420.01025732644330.7086930356610.01182822453890.649134890649-6.3435344050924.8293862181-17.0435578033
56.958111853480.2100917662555.42620891962.16081622754-0.3630221786354.53331523896-0.4919662627090.1160317933230.5157114302040.0904648036821-0.165374095822-0.238249380323-0.8851774977480.2687798144290.5559345014140.6125659579660.0222292725630.07947749263480.752145220360.04387589124720.671891579184-6.6842065241934.9296236609-10.4374722468
67.30479326737-0.06012832593280.4390462105126.250007642460.6951233079685.832748882490.0961367632769-1.00144579787-0.8231230669781.00033640030.00769810508217-0.820733686620.5760228075850.539933821754-0.1478749527450.5992967477880.0472844923778-0.1232732918970.8112080371260.02721513448810.602658481276-5.518264336427.44035043236.13412205024
76.339537121232.16190816017-0.1602658011093.97719627077-0.03818694033132.527308115760.492318491823-1.133781225270.1938961526520.757986082321-0.5470944546970.284022700882-0.425343697115-0.2158905202820.05821674335130.958408258106-0.009328310637370.003718629870691.06219521177-0.1453815429640.801328763202-10.006707113232.74779550414.8292799929
85.13855601394-1.766875810372.406567570713.42398425553-0.2861001642925.43239254768-0.324107816958-0.6866508693050.750559141894-0.0830684956174-0.0538793936739-0.264336664655-0.5680856323230.2209785486660.1816099697350.3744873808720.07266915385-0.0526440330060.570300061376-0.1181213127950.551174258435-15.142703524936.2424291441-4.07170014437
97.08270415575-0.855516697908-0.75268017219.75753965671-5.997508329979.4348341211-0.895354736753-0.329935054054-0.888351029828-0.4966734872020.29718952351.526248633351.01179875319-2.216982859050.3838363064750.42541851739-0.0437175316887-0.1132672843260.965985815435-0.1669681337340.858021478984-28.903524781226.4113940222-9.52965773389
104.640152724554.590948491831.020561648935.50052657944-0.4054545552382.779882739790.147666423283-0.572562283175-0.4118903012180.27387994661-0.0960625357583-0.05593705783080.188307859366-1.08457351953-0.1126883802110.5839399436250.1856084624190.04905441281510.61343950069-0.0201887312830.636691172612-13.175696916-21.397632659-4.10964398761
113.061369634833.680966892541.673922213788.89346043694.31784267434.028220953311.26028098695-1.623629433391.58920537659-0.11354698620.30683619322-0.06360061063450.890208601503-0.367966999447-1.751334307811.15048388182-0.1532724709680.2849091018921.17398252473-0.3447091038390.904181532031-29.853107816318.64820694133.87530055732
122.867831151890.00280816567379-2.682229672612.305871141961.124581852193.04490671276-0.1362788679170.5584575485571.22090824669-0.1661583995370.5060934357660.522538842837-0.112831475263-0.573489299266-0.4227948746360.8572811850760.0985012944251-0.05919136887360.740547341215-0.05737228696740.781742026696-21.131818323-13.613954506-30.2453675681
134.64325537630.7436847641945.241226688320.9046069545131.350799327766.80710612608-1.11717322571-1.175220860561.24042341857-0.3393354312160.2170883047730.308398991181-1.41502919717-1.039944796320.9795264076781.096853598370.229778267299-0.06636446258270.930140548494-0.1390347013341.25951890002-6.2130092261-1.84094442371-12.9603686711
144.639610018392.48365389170.5043070356585.98397956948-0.858181461654.43935833929-0.213539404082-0.3436008292020.4566653725590.233232677721-0.01710644816730.044054626337-0.159691072393-0.1784219640860.2076896538710.423476178860.1093445648-0.04710807567890.436421360154-0.05115562385240.473117168512-8.59145662371-16.5765273592-15.3587588432
159.16009214958-1.437647325281.389036119075.076703429110.02549905485115.2041456742-0.2863455308040.9978287138230.1503787373510.123179700060.363881475382-0.363391546291-0.8940549182771.45679251461-0.08313732353610.92390792212-0.150058813395-0.1340873530690.711745689364-0.09421805595580.7582530653656.54330959145-1.78657599498-19.8216247629
165.651776098770.9767930976084.830549138890.995159426771.180692542325.20016222219-0.07308932556640.906331135428-0.159340006425-0.06892502270310.192108147847-0.254612493779-0.5179051721940.973605051310.04379551329940.786809144643-0.198419693328-0.05078721261610.8342560422580.1040743351110.6991854743467.54843320716-12.0696070807-26.1427447848
174.231679780820.489869501691-2.615419567174.3189650057-0.7620300665715.59583265113-0.3976696839750.8068322028290.112303697847-0.2809716673780.5187445605290.1230122235560.123611346077-0.770666508951-0.1117562301730.550899683215-0.00954897741022-0.102032219550.664922634840.01261718437890.561000300923-12.5200958719-21.8633788617-30.268371885
185.00424392071-0.9861055887973.654404013052.08549739847-1.224729631477.15720197923-0.217420902264-0.233746640766-0.17468366296-0.4688124066440.341628864621-0.004097415295610.343547818262-0.356880730914-0.08670536519250.538817455697-0.0115302100124-0.06742907999390.570861081522-0.04107274983950.5268257911113.37119392061-20.8304403125-21.75784911
192.18737595460.6233232061741.944609093295.378768515520.7392285478578.15724400283-0.223037796858-1.08687015495-0.3020768385591.209631519830.234443094246-0.0723210626485-0.00775159664711-0.204145810718-0.04667192887640.6934373444840.1510697677-0.06035367197030.8840119167190.03335665761320.72033184112.42236619868-19.4444802761-3.64428880218
209.35026669028-2.14484144352-3.538796464478.02216894027-1.946098990433.331305951980.781307951815-1.37199786781-0.2720635869310.0973846736111-0.05325247917590.0202214364513-0.01878775039170.165468612335-0.6856220438380.9735127582070.00457313122247-0.05432469503060.99969834920.08994550679910.670694412482-4.8474496678319.568444048112.3345527933
217.66433910682-2.272578348693.334065741597.90639243239-3.27733755722.224415509611.010566984260.672818786913-1.67082973040.039820513791-0.716547484769-0.06667953277971.583466155210.686130770239-0.2700699213561.022877344810.09393125664080.0484368356230.91540358326-0.03057711103861.16985762197-17.323618753612.6865220094-11.5877184894
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'B' and (resid 260 through 282 )
2X-RAY DIFFRACTION2chain 'B' and (resid 283 through 301 )
3X-RAY DIFFRACTION3chain 'B' and (resid 302 through 316 )
4X-RAY DIFFRACTION4chain 'B' and (resid 317 through 341 )
5X-RAY DIFFRACTION5chain 'B' and (resid 342 through 360 )
6X-RAY DIFFRACTION6chain 'B' and (resid 361 through 376 )
7X-RAY DIFFRACTION7chain 'B' and (resid 377 through 414 )
8X-RAY DIFFRACTION8chain 'B' and (resid 415 through 442 )
9X-RAY DIFFRACTION9chain 'B' and (resid 443 through 459 )
10X-RAY DIFFRACTION10chain 'C' and (resid 741 through 752 )
11X-RAY DIFFRACTION11chain 'D' and (resid 741 through 751 )
12X-RAY DIFFRACTION12chain 'A' and (resid 221 through 233 )
13X-RAY DIFFRACTION13chain 'A' and (resid 234 through 261 )
14X-RAY DIFFRACTION14chain 'A' and (resid 262 through 316 )
15X-RAY DIFFRACTION15chain 'A' and (resid 317 through 341 )
16X-RAY DIFFRACTION16chain 'A' and (resid 342 through 360 )
17X-RAY DIFFRACTION17chain 'A' and (resid 361 through 414 )
18X-RAY DIFFRACTION18chain 'A' and (resid 415 through 442 )
19X-RAY DIFFRACTION19chain 'A' and (resid 443 through 459 )
20X-RAY DIFFRACTION20chain 'B' and (resid 221 through 236 )
21X-RAY DIFFRACTION21chain 'B' and (resid 237 through 259 )

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