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- PDB-8clh: Drug cocktail (Colchicine, Epothilone A, Peloruside, Ansamitocin ... -

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Basic information

Entry
Database: PDB / ID: 8clh
TitleDrug cocktail (Colchicine, Epothilone A, Peloruside, Ansamitocin P3, Vinblastine) bound to tubulin (T2R-TTL) complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin tyrosine ligase
KeywordsCELL CYCLE / Drug-tubulin-complex Cell cycle inhibition
Function / homology
Function and homology information


tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding / spindle microtubule / protein modification process ...tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding / spindle microtubule / protein modification process / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / neuron projection development / mitotic cell cycle / double-stranded RNA binding / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / neuron projection / cilium / protein heterodimerization activity / nucleotide binding / GTPase activity / ubiquitin protein ligase binding / GTP binding / Golgi apparatus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. ...Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / Chem-BKF / EPOTHILONE A / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Chem-LOC / Peloruside A / Chem-VLB / Tubulin tyrosine ligase / Stathmin-4 ...PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / Chem-BKF / EPOTHILONE A / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Chem-LOC / Peloruside A / Chem-VLB / Tubulin tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (domestic cattle)
MethodX-RAY DIFFRACTION / FREE ELECTRON LASER / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsWranik, M. / Bertrand, Q. / Kepa, M.W. / Weinert, T. / Steinmetz, M. / Standfuss, J.
Funding support Switzerland, 3items
OrganizationGrant numberCountry
Swiss National Science Foundation31003A_179351 Switzerland
Swiss National Science Foundation310030_207462 Switzerland
Swiss National Science Foundation310030_192566 Switzerland
CitationJournal: Nat Commun / Year: 2023
Title: A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers.
Authors: Wranik, M. / Kepa, M.W. / Beale, E.V. / James, D. / Bertrand, Q. / Weinert, T. / Furrer, A. / Glover, H. / Gashi, D. / Carrillo, M. / Kondo, Y. / Stipp, R.T. / Khusainov, G. / Nass, K. / ...Authors: Wranik, M. / Kepa, M.W. / Beale, E.V. / James, D. / Bertrand, Q. / Weinert, T. / Furrer, A. / Glover, H. / Gashi, D. / Carrillo, M. / Kondo, Y. / Stipp, R.T. / Khusainov, G. / Nass, K. / Ozerov, D. / Cirelli, C. / Johnson, P.J.M. / Dworkowski, F. / Beale, J.H. / Stubbs, S. / Zamofing, T. / Schneider, M. / Krauskopf, K. / Gao, L. / Thorn-Seshold, O. / Bostedt, C. / Bacellar, C. / Steinmetz, M.O. / Milne, C. / Standfuss, J.
History
DepositionFeb 16, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 13, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)260,69723
Polymers254,7006
Non-polymers5,99717
Water2,270126
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area25300 Å2
ΔGint-171 kcal/mol
Surface area81560 Å2
MethodPISA
Unit cell
Length a, b, c (Å)107.000, 160.340, 181.920
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain


Mass: 48966.324 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 48260.215 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16282.527 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: P63043
#4: Protein Tubulin tyrosine ligase


Mass: 43964.059 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: E1BQ43

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Non-polymers , 11 types, 143 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#7: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: Ca
#8: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#9: Chemical ChemComp-LOC / N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide / COLCHICINE


Mass: 399.437 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C22H25NO6 / Feature type: SUBJECT OF INVESTIGATION
#10: Chemical ChemComp-EP / EPOTHILONE A


Mass: 493.656 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C26H39NO6S / Feature type: SUBJECT OF INVESTIGATION
#11: Chemical ChemComp-POU / Peloruside A / (1R,3R,4S,7S,9S,11S,13R,14R,15R)-4,11,13,14-tetrahydroxy-7-[(2Z,4R)-4-(hydroxymethyl)hex-2-en-2-yl]-3,9,15-trimethoxy-12,12-dimethyl-6,17-dioxabicyclo[11.3.1]heptadecan-5-one


Mass: 548.663 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C27H48O11 / Feature type: SUBJECT OF INVESTIGATION
#12: Chemical ChemComp-VLB / (2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE / VINBLASTINE


Mass: 810.974 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C46H58N4O9 / Feature type: SUBJECT OF INVESTIGATION
#13: Chemical ChemComp-BKF / (1S,2S,3S,5S,6S,16Z,18Z,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl 2-methylpropanoate


Mass: 635.145 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C32H43ClN2O9 / Feature type: SUBJECT OF INVESTIGATION
#14: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#15: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 126 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.16 Å3/Da / Density % sol: 61.06 %
Crystal growTemperature: 293 K / Method: small tubes / pH: 6.5
Details: 6% PEG4000 (w/v), 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine

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Data collection

DiffractionMean temperature: 293 K / Serial crystal experiment: Y
Diffraction sourceSource: FREE ELECTRON LASER / Site: SwissFEL ARAMIS / Beamline: ESA / Wavelength: 1.03 Å
DetectorType: PSI JUNGFRAU 16M / Detector: PIXEL / Date: Feb 28, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03 Å / Relative weight: 1
ReflectionResolution: 2.4→15.29 Å / Num. obs: 121575 / % possible obs: 100 % / Redundancy: 350.9 % / CC1/2: 0.98 / Net I/σ(I): 6.15
Reflection shellResolution: 2.4→2.5 Å / Mean I/σ(I) obs: 0.77 / Num. unique obs: 12016 / CC1/2: 0.321
Serial crystallography sample deliveryMethod: injection
Serial crystallography sample delivery injectionCarrier solvent: Hydroxyethylcellulose / Description: High-viscosity injection / Injector diameter: 75 µm

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Processing

Software
NameVersionClassification
PHENIX1.20_4487refinement
CrystFEL0.8.0data reduction
CrystFEL0.8.0data scaling
PHENIX1.20_4487phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→15.29 Å / SU ML: 0.34 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 25.18 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.239 1182 1.09 %
Rwork0.1849 --
obs0.1854 108000 99.91 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.5→15.29 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17316 0 395 126 17837
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004
X-RAY DIFFRACTIONf_angle_d1.087
X-RAY DIFFRACTIONf_dihedral_angle_d17.9326853
X-RAY DIFFRACTIONf_chiral_restr0.0582708
X-RAY DIFFRACTIONf_plane_restr0.0053312
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5-2.610.35311460.3113201X-RAY DIFFRACTION100
2.61-2.750.30721460.293913216X-RAY DIFFRACTION100
2.75-2.920.36841480.268913258X-RAY DIFFRACTION100
2.92-3.150.25451460.239613300X-RAY DIFFRACTION100
3.15-3.460.21411470.205513301X-RAY DIFFRACTION100
3.46-3.950.25041480.174413376X-RAY DIFFRACTION100
3.95-4.950.19621480.142313445X-RAY DIFFRACTION100
4.95-15.290.22161530.155713721X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.6154-0.2198-0.43926.01271.46813.93170.1364-0.0790.6891-0.2640.0317-0.3103-0.88660.2261-0.17410.7512-0.06720.12310.6041-0.12380.624129.97898.940554.0525
21.6155-0.127-0.21572.8880.89222.38310.06120.0180.1073-0.15750.1406-0.2348-0.21930.3767-0.19690.4589-0.00840.01350.5156-0.17390.50929.402383.647155.9608
31.14240.9564-1.11362.56040.53524.32440.1916-0.18470.13370.3729-0.02910.6558-0.2832-0.2651-0.1530.54060.02260.04520.481-0.17550.591814.682486.061571.3884
41.0979-0.65421.24476.5459-0.08972.5479-0.1625-0.25370.33151.21760.15760.2856-0.0931-0.079-0.08040.84410.02590.12530.6241-0.17450.58118.023589.49580.8561
53.5401-0.60860.31436.06120.90934.696-0.0248-0.23220.06110.8650.12050.2387-0.16630.2017-0.0630.5968-0.01260.12970.5873-0.16050.587218.872290.477574.5584
61.3269-0.3354-1.35153.72212.00424.004-0.15-0.0723-0.26090.34850.21480.07120.72470.4452-0.00610.5260.1208-0.05710.523-0.13120.527127.530962.706957.7575
75.7741-1.26990.5968.42385.02385.4653-0.2032-0.6793-0.42681.13430.2099-0.04331.19440.61920.04140.71610.182-0.13720.5887-0.03130.586232.021864.900470.2289
87.6232-1.918-0.67239.11971.90935.0530.1599-0.08750.8479-0.5489-0.07680.4088-0.8673-0.1308-0.07980.5920.00980.04370.39250.00530.483315.88574.092922.6417
96.9799-3.1297-0.7767.50015.30597.77980.09590.66180.7599-1.2869-0.42560.3698-1.209-0.31120.30050.72950.0564-0.03120.54170.06730.502518.581169.001312.6142
102.52390.27240.05284.6596-0.13032.44880.03490.44440.1495-0.3850.3141-0.4747-0.47040.4732-0.35530.6237-0.07960.12350.6567-0.11550.546329.327958.348314.2704
110.92110.34730.40291.54090.72842.0978-0.0057-0.1570.1651-0.04130.00170.0102-0.13150.0108-0.00490.44850.01390.01440.4842-0.07590.48317.181355.935526.5369
126.9287-0.47832.07550.3992-1.29644.6074-0.0149-0.08020.450.1078-0.12150.0248-0.07560.12150.13940.42470.01630.05720.4281-0.1760.463319.759663.799639.6517
133.4897-1.0846-1.00027.80591.05124.67710.03960.24030.0361-0.4371-0.1941.4156-0.2762-1.22280.04670.45190.0109-0.03690.725-0.1530.6925-1.007458.834837.9467
144.858-1.9222-1.35486.52130.04885.8694-0.1398-0.67920.8060.59150.31680.3134-0.7077-1.2623-0.15480.54150.11240.00350.7843-0.24570.62085.040567.567646.8503
151.3813-0.8091-0.44571.74442.33543.5837-0.1186-0.02340.23570.404-0.0630.04030.2775-0.11560.17570.3718-0.02870.01940.5155-0.09950.453311.943954.290339.0771
161.4148-0.9179-1.69614.9193.93487.5437-0.2312-0.1548-0.2030.78080.1743-0.1841.03780.6580.05240.44730.0449-0.07620.4333-0.02830.48524.856440.175431.8191
174.8625-2.1342-1.58415.03770.82374.0146-0.05760.19110.4982-0.18050.06260.1873-0.687-0.05390.01860.5745-0.056-0.01830.4270.03430.402713.597243.5759-13.3756
181.4297-0.29730.07272.38790.39152.03930.01620.1888-0.0264-0.25990.06290.002-0.10640.1021-0.07510.4489-0.05020.01590.4359-0.06340.407717.589528.3426-10.2674
194.2624-2.3561.99581.9594-1.31472.88390.01130.10330.26890.0429-0.0025-0.0161-0.1092-0.08380.00720.3547-0.010.06860.4411-0.08020.492710.587135.21965.9294
201.1072-1.38350.67212.51910.41052.2060.0038-0.023-0.2004-0.0434-0.12440.38640.108-0.52310.0820.4118-0.1070.02460.567-0.10930.527-2.7429.97955.5901
218.2742-7.03633.76449.6411-3.63823.0861-0.336-0.7273-0.38130.16840.490.90370.2921-0.558-0.1080.4805-0.04690.08640.5579-0.04350.50550.95432.342118.2112
221.8576-0.8746-0.47772.17861.08262.1268-0.0955-0.0749-0.08930.13450.11510.00030.23970.1578-0.03140.4001-0.07450.01780.3415-0.03990.398515.113320.19971.5871
235.9919-2.8340.14835.52152.2572.7701-0.05530.63570.3638-0.712-0.02410.1166-0.1324-0.12130.07220.7746-0.14550.06620.80630.01070.435714.418412.4626-41.7479
242.804-2.428-1.6334.93662.8751.71890.39170.84550.0841-1.4428-0.516-0.141-0.2401-0.53930.13581.2115-0.0550.10741.2184-0.19010.53720.8975.5151-49.5866
251.1233-1.78260.51357.19511.10162.5633-0.14220.9986-0.2981-0.72650.0885-0.80930.34230.41140.02920.8203-0.13650.31721.1651-0.28370.740133.49770.0959-44.3927
262.76330.2963-0.08632.65260.17062.8543-0.21530.4106-0.4852-0.53570.2262-0.23220.3451-0.0513-0.00840.6875-0.08790.10250.6275-0.20940.519620.4038-4.7625-34.1945
272.5079-0.04071.88822.6197-2.1956.32150.08690.03590.1841-0.01280.2348-0.3074-0.17190.0212-0.33490.5409-0.09840.10380.5182-0.11830.512717.67227.4784-22.8676
281.0661-0.8871-1.76454.03510.83963.36130.06140.9458-0.6812-0.3834-0.1730.9560.5605-1.22860.08630.7748-0.2335-0.02361.0247-0.23930.8162-3.6816-2.8197-28.7333
292.8605-1.015-0.07952.99410.16941.7185-0.18440.2268-0.38140.02480.09640.15430.4638-0.33810.11580.6361-0.14470.06710.5512-0.13590.52728.8175-3.3146-21.0776
305.3289-0.5285-2.03012.5277-1.78913.336-0.23420.0929-0.7143-0.00480.1014-0.23820.92230.60010.02560.78440.05360.01010.606-0.23730.749230.5459-15.7343-24.4707
317.70880.19020.32493.4137-4.48746.0811-0.0273-0.52890.3741.0319-0.011-0.2328-1.63311.27030.0591.1418-0.22450.02890.8948-0.25010.733326.7519101.062282.154
320.63590.44730.24330.39360.14460.0958-0.2527-0.3631-0.27010.92240.2313-0.42560.62151.1110.14871.43810.1562-0.23751.2868-0.26380.749532.683984.102582.7692
330.3397-0.0085-0.25663.43833.76354.3771-0.2196-0.0006-0.03770.59430.6024-0.3150.72730.9192-0.49740.52490.09180.01010.7576-0.1940.731142.801329.02093.8061
343.6756-0.3666-1.476-0.0095-0.12264.9623-0.1230.5573-0.7004-0.03910.0006-0.11971.4239-0.32640.08951.1234-0.09320.08540.6173-0.11010.70067.741558.123774.3747
357.3508-1.34514.05327.7665-2.49092.6966-0.0808-0.79660.83690.2165-0.3641-0.951-0.39211.01640.6631.02970.0967-0.05271.1886-0.0250.776614.462167.5335102.3524
365.14461.52632.80373.4126-2.55665.8366-0.3376-1.74620.35960.8383-0.5406-0.6837-0.55561.56720.49551.3360.1956-0.22191.83580.12311.073618.436361.3459113.9987
376.86650.35190.69145.3769-0.43894.0657-0.3704-0.4724-1.03580.6675-0.1157-1.24181.12591.20570.39341.43140.39270.02411.18470.30871.175814.029452.094106.9735
384.70481.1275-1.68434.5228-2.44449.0107-0.50310.2388-0.63-0.47180.24710.37421.816-0.33550.24711.0614-0.04780.21230.6769-0.0420.7722-3.119958.101989.741
392.06680.2649-2.1771.57810.10672.3896-0.2118-0.2971-0.9073-0.1437-0.4433-1.48791.70620.72840.51781.63150.36430.21431.48530.45511.64335.426542.9267105.6801
403.2102-0.2554-3.10521.64260.01936.3042-0.42890.144-0.53230.19340.36320.17550.983-0.24770.05291.0082-0.00340.1220.60810.0280.7036-2.837758.801593.3444
418.3188-4.8676-4.84946.8861.0723.7176-0.18080.6738-0.1284-0.07810.66880.0040.3583-1.1803-0.50550.7424-0.1115-0.00120.78960.00020.9228-7.892564.217379.35
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 72 )
2X-RAY DIFFRACTION2chain 'A' and (resid 73 through 259 )
3X-RAY DIFFRACTION3chain 'A' and (resid 260 through 306 )
4X-RAY DIFFRACTION4chain 'A' and (resid 307 through 337 )
5X-RAY DIFFRACTION5chain 'A' and (resid 338 through 381 )
6X-RAY DIFFRACTION6chain 'A' and (resid 382 through 414 )
7X-RAY DIFFRACTION7chain 'A' and (resid 415 through 439 )
8X-RAY DIFFRACTION8chain 'B' and (resid 2 through 56 )
9X-RAY DIFFRACTION9chain 'B' and (resid 57 through 88 )
10X-RAY DIFFRACTION10chain 'B' and (resid 89 through 128 )
11X-RAY DIFFRACTION11chain 'B' and (resid 129 through 243 )
12X-RAY DIFFRACTION12chain 'B' and (resid 244 through 273 )
13X-RAY DIFFRACTION13chain 'B' and (resid 274 through 311 )
14X-RAY DIFFRACTION14chain 'B' and (resid 312 through 338 )
15X-RAY DIFFRACTION15chain 'B' and (resid 339 through 401 )
16X-RAY DIFFRACTION16chain 'B' and (resid 402 through 440 )
17X-RAY DIFFRACTION17chain 'C' and (resid 1 through 72 )
18X-RAY DIFFRACTION18chain 'C' and (resid 73 through 243 )
19X-RAY DIFFRACTION19chain 'C' and (resid 244 through 277 )
20X-RAY DIFFRACTION20chain 'C' and (resid 278 through 324 )
21X-RAY DIFFRACTION21chain 'C' and (resid 325 through 350 )
22X-RAY DIFFRACTION22chain 'C' and (resid 351 through 440 )
23X-RAY DIFFRACTION23chain 'D' and (resid 2 through 59 )
24X-RAY DIFFRACTION24chain 'D' and (resid 60 through 88 )
25X-RAY DIFFRACTION25chain 'D' and (resid 89 through 127 )
26X-RAY DIFFRACTION26chain 'D' and (resid 128 through 238 )
27X-RAY DIFFRACTION27chain 'D' and (resid 239 through 268 )
28X-RAY DIFFRACTION28chain 'D' and (resid 269 through 296 )
29X-RAY DIFFRACTION29chain 'D' and (resid 297 through 401 )
30X-RAY DIFFRACTION30chain 'D' and (resid 402 through 441 )
31X-RAY DIFFRACTION31chain 'E' and (resid 6 through 20 )
32X-RAY DIFFRACTION32chain 'E' and (resid 21 through 46 )
33X-RAY DIFFRACTION33chain 'E' and (resid 47 through 143 )
34X-RAY DIFFRACTION34chain 'F' and (resid 1 through 83 )
35X-RAY DIFFRACTION35chain 'F' and (resid 84 through 127 )
36X-RAY DIFFRACTION36chain 'F' and (resid 128 through 151 )
37X-RAY DIFFRACTION37chain 'F' and (resid 152 through 198 )
38X-RAY DIFFRACTION38chain 'F' and (resid 199 through 223 )
39X-RAY DIFFRACTION39chain 'F' and (resid 224 through 257 )
40X-RAY DIFFRACTION40chain 'F' and (resid 258 through 353 )
41X-RAY DIFFRACTION41chain 'F' and (resid 354 through 381 )

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