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- PDB-8biy: Citrate-free extracytoplasmic PAS domain mutant R93A of sensor hi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8biy | ||||||
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Title | Citrate-free extracytoplasmic PAS domain mutant R93A of sensor histidine kinase CitA from Geobacillus thermodenitrificans | ||||||
![]() | Histidine kinase | ||||||
![]() | SIGNALING PROTEIN / signal transduction / Geobacillus thermodenitrificans / sensor histidine kinase / CitA / sensor domain / extracytoplasmic PAS domain / R93A mutation / two-component signaling system | ||||||
Function / homology | ![]() histidine kinase / phosphorelay sensor kinase activity / nucleotide binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Becker, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Citrate-free extracytoplasmic PAS domain mutant R93A of sensor histidine kinase CitA from Geobacillus thermodenitrificans Authors: Becker, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.9 KB | Display | ![]() |
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PDB format | ![]() | 92.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 493.2 KB | Display | ![]() |
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Full document | ![]() | 496.1 KB | Display | |
Data in XML | ![]() | 23 KB | Display | |
Data in CIF | ![]() | 32.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8bgbS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14040.037 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: R93A mutant Source: (gene. exp.) ![]() Gene: dctS_2, GTHT12_01458 / Production host: ![]() ![]() #2: Chemical | ChemComp-CXS / #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 10.6 Details: 0.2M lithium sulfate, 0.9M sodium di-hydrogen phosphate, 0.6M di-potassium hydrogen phosphate, 0.1M N-cyclohexyl-3-aminopropanesulfonic acid |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.61→47.12 Å / Num. obs: 70580 / % possible obs: 99.1 % / Redundancy: 12.94 % / Biso Wilson estimate: 27.74 Å2 / Rrim(I) all: 0.049 / Net I/σ(I): 16.33 |
Reflection shell | Resolution: 1.61→1.64 Å / Num. unique obs: 2137 / Rrim(I) all: 0.63 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 8BGB Resolution: 1.61→47.12 Å / SU ML: 0.1865 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 23.4588 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.36 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.61→47.12 Å
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Refine LS restraints |
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LS refinement shell |
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