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Yorodumi- PDB-8bb7: Crystal structure of Mouse Plexin-B1 (20-535) in complex with VHH15 -
+Open data
-Basic information
Entry | Database: PDB / ID: 8bb7 | ||||||
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Title | Crystal structure of Mouse Plexin-B1 (20-535) in complex with VHH15 | ||||||
Components |
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Keywords | SIGNALING PROTEIN / Complex / inhibitor / VHH / nanobody / Plexin / semaphorin / allosteric | ||||||
Function / homology | Function and homology information semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis / Sema4D mediated inhibition of cell attachment and migration / Sema4D induced cell migration and growth-cone collapse / RHOD GTPase cycle / : / semaphorin-plexin signaling pathway involved in axon guidance / G alpha (12/13) signalling events / negative regulation of osteoblast proliferation / inhibitory synapse assembly / semaphorin receptor complex ...semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis / Sema4D mediated inhibition of cell attachment and migration / Sema4D induced cell migration and growth-cone collapse / RHOD GTPase cycle / : / semaphorin-plexin signaling pathway involved in axon guidance / G alpha (12/13) signalling events / negative regulation of osteoblast proliferation / inhibitory synapse assembly / semaphorin receptor complex / semaphorin receptor activity / negative regulation of cell adhesion / GTPase activating protein binding / ossification involved in bone maturation / regulation of cytoskeleton organization / positive regulation of axonogenesis / regulation of GTPase activity / semaphorin-plexin signaling pathway / regulation of cell migration / neuron projection morphogenesis / positive regulation of GTPase activity / regulation of cell shape / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Camelidae (mammal) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.95 Å | ||||||
Authors | Cowan, R. / Hall, G. / Carr, M. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2023 Title: Nanobody inhibitors of Plexin-B1 identify allostery in plexin-semaphorin interactions and signaling. Authors: Cowan, R. / Trokter, M. / Oleksy, A. / Fedorova, M. / Sawmynaden, K. / Worzfeld, T. / Offermanns, S. / Matthews, D. / Carr, M.D. / Hall, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8bb7.cif.gz | 271.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8bb7.ent.gz | 197.2 KB | Display | PDB format |
PDBx/mmJSON format | 8bb7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/8bb7 ftp://data.pdbj.org/pub/pdb/validation_reports/bb/8bb7 | HTTPS FTP |
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-Related structure data
Related structure data | 8b3kC 8bf4C 3ol2S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 56450.527 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Plxnb1, Kiaa0407 / Cell line (production host): Expi293-T / Production host: Homo sapiens (human) / References: UniProt: Q8CJH3 #2: Antibody | Mass: 14258.775 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Huarizo / Source: (gene. exp.) Camelidae (mammal) / Production host: Homo sapiens (human) #3: Sugar | ChemComp-NAG / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.39 Å3/Da / Density % sol: 63.8 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 8% (w/v) PEG 8000, 0.1 M Sodium Citrate, pH 5.0, 1% (w/v) dextran-sulphate (Mr 5000) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.9184 Å | |||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 27, 2018 | |||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 | |||||||||||||||||||||
Reflection | Resolution: 2.948→47.194 Å / Num. obs: 36092 / % possible obs: 99.9 % / Redundancy: 6.4 % / Biso Wilson estimate: 42.66 Å2 / CC1/2: 0.953 / Rmerge(I) obs: 0.42 / Rpim(I) all: 0.274 / Rrim(I) all: 0.504 / Net I/σ(I): 5.6 | |||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3OL2 Resolution: 2.95→47.15 Å / SU ML: 0.3244 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.0364 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.18 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.95→47.15 Å
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Refine LS restraints |
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LS refinement shell |
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