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- PDB-8b3k: Crystal structure of human Plexin-B1 (20-535) in the unbound state -
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Open data
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Basic information
Entry | Database: PDB / ID: 8b3k | ||||||
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Title | Crystal structure of human Plexin-B1 (20-535) in the unbound state | ||||||
![]() | Plexin-B1 | ||||||
![]() | SIGNALING PROTEIN / receptor / unbound / guidance / adherence / neurodevelopment / axonogenesis | ||||||
Function / homology | ![]() semaphorin receptor binding / negative regulation of osteoblast proliferation / inhibitory synapse assembly / semaphorin-plexin signaling pathway involved in axon guidance / semaphorin receptor activity / negative regulation of cell adhesion / RHOD GTPase cycle / semaphorin receptor complex / axon extension / Sema4D induced cell migration and growth-cone collapse ...semaphorin receptor binding / negative regulation of osteoblast proliferation / inhibitory synapse assembly / semaphorin-plexin signaling pathway involved in axon guidance / semaphorin receptor activity / negative regulation of cell adhesion / RHOD GTPase cycle / semaphorin receptor complex / axon extension / Sema4D induced cell migration and growth-cone collapse / GTPase activating protein binding / ossification involved in bone maturation / Sema4D mediated inhibition of cell attachment and migration / positive regulation of axonogenesis / positive regulation of Rho protein signal transduction / regulation of cytoskeleton organization / semaphorin-plexin signaling pathway / regulation of cell migration / GTPase activator activity / neuron projection morphogenesis / positive regulation of GTPase activity / transmembrane signaling receptor activity / G alpha (12/13) signalling events / cell migration / regulation of cell shape / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / intracellular signal transduction / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cowan, R. / Hall, G. / Carr, M. | ||||||
Funding support | 1items
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![]() | ![]() Title: Nanobody inhibitors of Plexin-B1 identify allostery in plexin-semaphorin interactions and signaling. Authors: Cowan, R. / Trokter, M. / Oleksy, A. / Fedorova, M. / Sawmynaden, K. / Worzfeld, T. / Offermanns, S. / Matthews, D. / Carr, M.D. / Hall, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 208 KB | Display | ![]() |
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PDB format | ![]() | 161.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 467.2 KB | Display | ![]() |
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Full document | ![]() | 480.1 KB | Display | |
Data in XML | ![]() | 38.3 KB | Display | |
Data in CIF | ![]() | 54 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8bb7C ![]() 8bf4C ![]() 3ol2S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 56479.238 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-CD / #3: Sugar | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14150262 Å3/Da / Density % sol: 42.6 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 7.5 Details: 1.0 M sodium acetate, 0.2 M cadmium sulphate, 0.1 M HEPES, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 15, 2018 | |||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 | |||||||||||||||||||||
Reflection | Resolution: 2.685→188.58 Å / Num. obs: 48266 / % possible obs: 100 % / Redundancy: 13.5 % / CC1/2: 0.993 / Rmerge(I) obs: 0.374 / Rpim(I) all: 0.152 / Rrim(I) all: 0.403 / Net I/σ(I): 5.9 | |||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3OL2 Resolution: 2.685→66.67 Å / Cross valid method: FREE R-VALUE / Phase error: 24.4571
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.35 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.685→66.67 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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