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Open data
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Basic information
Entry | Database: PDB / ID: 8b8a | |||||||||
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Title | Multimerization domain of borna disease virus 1 phosphoprotein | |||||||||
![]() | Phosphoprotein | |||||||||
![]() | VIRAL PROTEIN / phosphoprotein / RNA polymerase cofactor | |||||||||
Function / homology | Borna disease virus P24 / Borna disease virus P24 protein / : / host cell cytoplasm / host cell nucleus / Phosphoprotein![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Tarbouriech, N. / Legrand, P. / Bourhis, J.M. / Chenavier, F. / Freslon, L. / Kawasaki, J. / Horie, M. / Tomonaga, K. / Bachiri, K. / Coyaud, E. ...Tarbouriech, N. / Legrand, P. / Bourhis, J.M. / Chenavier, F. / Freslon, L. / Kawasaki, J. / Horie, M. / Tomonaga, K. / Bachiri, K. / Coyaud, E. / Gonzalez-Dunia, D. / Ruigrok, R.W.H. / Crepin, T. | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Borna Disease Virus 1 Phosphoprotein Forms a Tetramer and Interacts with Host Factors Involved in DNA Double-Strand Break Repair and mRNA Processing. Authors: Tarbouriech, N. / Chenavier, F. / Kawasaki, J. / Bachiri, K. / Bourhis, J.M. / Legrand, P. / Freslon, L.L. / Laurent, E.M.N. / Suberbielle, E. / Ruigrok, R.W.H. / Tomonaga, K. / Gonzalez- ...Authors: Tarbouriech, N. / Chenavier, F. / Kawasaki, J. / Bachiri, K. / Bourhis, J.M. / Legrand, P. / Freslon, L.L. / Laurent, E.M.N. / Suberbielle, E. / Ruigrok, R.W.H. / Tomonaga, K. / Gonzalez-Dunia, D. / Horie, M. / Coyaud, E. / Crepin, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 52.9 KB | Display | ![]() |
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PDB format | ![]() | 36.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 417.8 KB | Display | ![]() |
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Full document | ![]() | 418 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 6.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8b8bC ![]() 8b8dC ![]() 4bhvS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12223.003 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 42 % / Description: fine plates |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 100 mM HEPES pH 7.5, 14-18 % polyethylene glycol 8K, 200 mM CaCl2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 24, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97717 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→55 Å / Num. obs: 3173 / % possible obs: 99.9 % / Redundancy: 11.1 % / Biso Wilson estimate: 80.59 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.258 / Rpim(I) all: 0.108 / Net I/σ(I): 5 |
Reflection shell | Resolution: 2.75→2.82 Å / Redundancy: 11.8 % / Rmerge(I) obs: 3.26 / Num. unique obs: 214 / CC1/2: 0.601 / Rpim(I) all: 1.357 / % possible all: 99.7 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4bhv Resolution: 2.75→20 Å / Cor.coef. Fo:Fc: 0.927 / Cor.coef. Fo:Fc free: 0.855 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.507
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Displacement parameters | Biso max: 145.5 Å2 / Biso mean: 80.02 Å2 / Biso min: 28.55 Å2
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Refine analyze | Luzzati coordinate error obs: 0.54 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.75→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.75→3 Å / Rfactor Rfree error: 0 / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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