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Open data
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Basic information
Entry | Database: PDB / ID: 8b8b | |||||||||
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Title | Multimerization domain of Munia virus 1 phosphoprotein | |||||||||
![]() | Munia Bornavirus 1 phosphoprotein | |||||||||
![]() | VIRAL PROTEIN / phosphoprotein / RNA polymerase cofactor | |||||||||
Function / homology | NITRATE ION![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Chenavier, F. / Tarbouriech, N. / Bourhis, J.M. / Tomonaga, K. / Horie, M. / Crepin, T. | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Borna Disease Virus 1 Phosphoprotein Forms a Tetramer and Interacts with Host Factors Involved in DNA Double-Strand Break Repair and mRNA Processing. Authors: Tarbouriech, N. / Chenavier, F. / Kawasaki, J. / Bachiri, K. / Bourhis, J.M. / Legrand, P. / Freslon, L.L. / Laurent, E.M.N. / Suberbielle, E. / Ruigrok, R.W.H. / Tomonaga, K. / Gonzalez- ...Authors: Tarbouriech, N. / Chenavier, F. / Kawasaki, J. / Bachiri, K. / Bourhis, J.M. / Legrand, P. / Freslon, L.L. / Laurent, E.M.N. / Suberbielle, E. / Ruigrok, R.W.H. / Tomonaga, K. / Gonzalez-Dunia, D. / Horie, M. / Coyaud, E. / Crepin, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 88.5 KB | Display | ![]() |
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PDB format | ![]() | 67.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 466.3 KB | Display | ![]() |
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Full document | ![]() | 471.3 KB | Display | |
Data in XML | ![]() | 16.3 KB | Display | |
Data in CIF | ![]() | 22.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8b8aC ![]() 8b8dC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12342.133 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.91 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 15-18 % PEG 3350, 150 mM Mg(NO3)2, 10 mM phenol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 8, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978565 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→49.34 Å / Num. obs: 23663 / % possible obs: 99.9 % / Redundancy: 5.4 % / CC1/2: 0.998 / Rmerge(I) obs: 0.052 / Rpim(I) all: 0.037 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 2.15→2.22 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.67 / Mean I/σ(I) obs: 2 / Num. unique obs: 2043 / CC1/2: 0.808 / Rpim(I) all: 0.471 / % possible all: 100 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: D_1292125760 Resolution: 2.15→49.34 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.893 / SU B: 7.697 / SU ML: 0.195 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.262 / ESU R Free: 0.254 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 135.76 Å2 / Biso mean: 54.702 Å2 / Biso min: 22.98 Å2
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Refinement step | Cycle: final / Resolution: 2.15→49.34 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.206 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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