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- PDB-8b7c: Tubulin-maytansinoid-12 complex -

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Basic information

Entry
Database: PDB / ID: 8b7c
TitleTubulin-maytansinoid-12 complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin tyrosine ligase
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE
Function / homology
Function and homology information


tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / tubulin binding / cellular response to interleukin-4 ...tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / tubulin binding / cellular response to interleukin-4 / positive regulation of mitotic cell cycle / post-translational protein modification / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / neuron projection development / mitotic cell cycle / double-stranded RNA binding / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / neuron projection / cilium / protein heterodimerization activity / GTPase activity / ubiquitin protein ligase binding / GTP binding / Golgi apparatus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Rossmann fold - #11480 / Helix hairpin bin / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family ...: / Rossmann fold - #11480 / Helix hairpin bin / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Tubulin/FtsZ, C-terminal domain / Tubulin/FtsZ, GTPase domain / ATP-grasp fold, B domain / D-amino Acid Aminotransferase; Chain A, domain 1 / 60s Ribosomal Protein L30; Chain: A; / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Helix Hairpins / Rossmann fold / 2-Layer Sandwich / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / IMIDAZOLE / Chem-PWC / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (domestic cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.9 Å
AuthorsBoiarska, Z. / Perez-Pena, H. / Abel, A.-C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. ...Boiarska, Z. / Perez-Pena, H. / Abel, A.-C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Steinmetz, M.O. / Prota, A.E. / Pieraccini, S. / Passarella, D.
Funding supportEuropean Union, Switzerland, 2items
OrganizationGrant numberCountry
H2020 Marie Curie Actions of the European Commission860070 TubInTrainEuropean Union
Swiss National Science Foundation31003A_166608 Switzerland
CitationJournal: Chemistry / Year: 2023
Title: Maytansinol Functionalization: Towards Useful Probes for Studying Microtubule Dynamics.
Authors: Boiarska, Z. / Perez-Pena, H. / Abel, A.C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Prota, A.E. ...Authors: Boiarska, Z. / Perez-Pena, H. / Abel, A.C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Prota, A.E. / Pieraccini, S. / Passarella, D.
History
DepositionSep 29, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 23, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,35423
Polymers261,6316
Non-polymers3,72217
Water21,1861176
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area22950 Å2
ΔGint-166 kcal/mol
Surface area80460 Å2
MethodPISA
Unit cell
Length a, b, c (Å)104.866, 157.939, 180.570
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Brain / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Brain / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043
#4: Protein Tubulin tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8V0Z8P0

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Non-polymers , 9 types, 1193 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mg
#7: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-IMD / IMIDAZOLE


Mass: 69.085 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H5N2
#9: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#10: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#11: Chemical ChemComp-PWC / [(1~{S},2~{R},3~{S},5~{S},6~{S},16~{E},18~{E},20~{R},21~{S})-11-chloranyl-12,20-dimethoxy-2,5,9,16-tetramethyl-21-oxidanyl-8,23-bis(oxidanylidene)-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1^{10,14}.0^{3,5}]hexacosa-10(26),11,13,16,18-pentaen-6-yl] 4-[2-(2-azanylhydrazinyl)ethyl]benzoate


Mass: 738.226 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C37H44ClN5O9 / Feature type: SUBJECT OF INVESTIGATION
#12: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1176 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.8 Å3/Da / Density % sol: 56.6 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1.000029 Å
DetectorType: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Feb 23, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.000029 Å / Relative weight: 1
ReflectionResolution: 1.9→49.57 Å / Num. obs: 235350 / % possible obs: 99.93 % / Redundancy: 13.5 % / Biso Wilson estimate: 39.41 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.1195 / Rpim(I) all: 0.03355 / Rrim(I) all: 0.1242 / Net I/σ(I): 15.92
Reflection shellResolution: 1.9→1.968 Å / Redundancy: 13.2 % / Rmerge(I) obs: 3.954 / Mean I/σ(I) obs: 0.58 / Num. unique obs: 23217 / CC1/2: 0.238 / CC star: 0.62 / Rpim(I) all: 1.118 / Rrim(I) all: 4.111 / % possible all: 99.81

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
XDSdata reduction
PDB_EXTRACT3.28data extraction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS
Starting model: 5LXT

5lxt


Resolution: 1.9→49.57 Å / SU ML: 0.2845 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.567
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2115 11740 5 %
Rwork0.1826 222992 -
obs0.184 234732 99.68 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 56.95 Å2
Refinement stepCycle: LAST / Resolution: 1.9→49.57 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17351 0 229 1176 18756
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002418037
X-RAY DIFFRACTIONf_angle_d0.586324465
X-RAY DIFFRACTIONf_chiral_restr0.0422659
X-RAY DIFFRACTIONf_plane_restr0.00353168
X-RAY DIFFRACTIONf_dihedral_angle_d12.65356678
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.9-1.920.42213610.40736800X-RAY DIFFRACTION92.51
1.92-1.940.37043880.37077362X-RAY DIFFRACTION99.45
1.94-1.970.38423870.34267359X-RAY DIFFRACTION99.77
1.97-1.990.33073910.32587418X-RAY DIFFRACTION99.91
1.99-2.020.32233880.30237381X-RAY DIFFRACTION99.94
2.02-2.050.3053890.28947400X-RAY DIFFRACTION99.87
2.05-2.080.30653870.28427336X-RAY DIFFRACTION99.91
2.08-2.110.29293920.26717466X-RAY DIFFRACTION99.95
2.11-2.140.27393880.25097363X-RAY DIFFRACTION99.92
2.14-2.170.26133900.24167404X-RAY DIFFRACTION99.96
2.17-2.210.28033870.23137359X-RAY DIFFRACTION99.94
2.21-2.250.26013910.21987433X-RAY DIFFRACTION99.96
2.25-2.290.24943920.21757434X-RAY DIFFRACTION99.94
2.3-2.340.24393900.21287420X-RAY DIFFRACTION99.97
2.34-2.390.24663900.20157406X-RAY DIFFRACTION99.95
2.39-2.450.21693900.19587416X-RAY DIFFRACTION99.97
2.45-2.510.2353910.19347420X-RAY DIFFRACTION99.97
2.51-2.580.2343920.18817452X-RAY DIFFRACTION99.99
2.58-2.650.24173900.18557414X-RAY DIFFRACTION99.96
2.65-2.740.21893920.18837449X-RAY DIFFRACTION99.95
2.74-2.840.23353920.19297452X-RAY DIFFRACTION99.95
2.84-2.950.22833930.19487462X-RAY DIFFRACTION99.99
2.95-3.080.22363940.18367481X-RAY DIFFRACTION100
3.08-3.250.21243920.18067460X-RAY DIFFRACTION99.94
3.25-3.450.19923950.17597498X-RAY DIFFRACTION99.96
3.45-3.720.19583960.16347516X-RAY DIFFRACTION100
3.72-4.090.1673960.14217535X-RAY DIFFRACTION100
4.09-4.680.14953990.13177568X-RAY DIFFRACTION99.99
4.68-5.90.19314020.15287644X-RAY DIFFRACTION99.99
5.9-49.570.18874150.17097884X-RAY DIFFRACTION99.81
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.862355697008-0.083663274831-0.1357682609733.030239851541.617890606442.70708119745-0.008168230344660.08216704270510.0887641812955-0.2355849339510.247637697796-0.254064209007-0.4815660657120.302908628953-0.1728299130850.375500918509-0.07276883838750.06496678809030.346113179922-0.1095862401470.33149661951828.738673743286.040050247655.9289604174
21.302471998931.47714586903-0.1391773837251.917859168660.5032466701093.126914578330.132007919043-0.1174839299140.153566976860.309488471063-0.2601694311990.678420870570.0643168987843-0.6052960168270.09255335825430.466947134575-0.02601581638140.157074372820.440807200606-0.1614141551080.4880148297039.1251521718384.7921620471.5484965824
30.58985720826-0.0183233538057-0.00383561370393.067227931171.87372825322.666857209080.00665525849122-0.114012077008-0.003120564303710.706866622770.04780848726070.0778894153190.3039364810870.0674738146504-0.0511098237060.443517484046-0.02267125585420.04720011742530.339127475837-0.06929077380990.3383164047922.972110831977.038867861469.9083328651
43.01447780462-1.04669755378-0.2671260674022.948975727480.5868627371372.437636784010.09011290410170.2260550581730.55821921383-0.397521281997-0.08990494167590.098802020541-0.815060982291-0.1946779020030.006740451960840.4873637512220.06680339884890.02173566428750.377124754903-0.03651006105420.45565703340116.386096016270.145747572819.3901173932
51.16468717613-0.2823342237-0.3451367591432.257333795131.478609956593.226590067470.01215928308360.03120854212450.0991031062312-0.0239441484954-0.0951543085890.152433372509-0.209513846572-0.100543783416-0.01930968187640.2089062615360.005338079954070.01450594537730.327526589209-0.09485914131420.32812226017920.350874508956.072479992125.4184677382
60.443289024298-0.06338622116640.7520383586681.815494604341.026433217022.30749958149-0.0759927036942-0.1696301086110.09236917872080.48832386303-0.1775673824730.2680540712310.181078941749-0.526854487230.1160769405430.281378061703-0.05158893112160.08820117622120.415860740645-0.1095817149960.3725062807112.168957860552.294271192638.5294831613
70.961629808884-0.0187091273903-0.002335946569832.40753256160.3098767647471.69699740264-0.05193433735410.1126096621620.112296132525-0.294864385280.0984275386086-0.0865895798191-0.1538656571110.13660915509-0.0415454377240.24210301641-0.06190122913750.03651899643970.321526169211-0.03110667308340.30440523049520.352228189533.15121747-11.955568087
80.767485241932-0.2754248721430.03284178849911.77636285561.033557808811.65707262714-0.0236547540698-0.009065293783210.06937862117540.107942811852-0.07933440488010.1466125960340.113229354286-0.2194870032660.07394602015130.171577575638-0.06251785206140.04794210156430.27522670335-0.03194876323230.2913939814837.7800667642626.10025866923.13934600891
92.81681786351-0.611120288180.8780830812372.39505716852-0.09261949842621.91121578191-0.1220903852080.5811504834460.113526033505-0.6947034483720.11095868454-0.01690339473120.0116184188835-0.1246789481250.01490859313420.62188546006-0.1096190486780.04184341052860.629632261012-0.05995357170610.29626197269317.44125702589.9499338379-43.9927501252
101.22600273153-0.175790591911-0.3505203813291.517188266940.3297584238432.11104795599-0.1262063843610.350805740002-0.202095779683-0.4805368159780.186131590827-0.2654310232620.2927521433570.178722704618-0.06405473844650.545577215683-0.04343166380270.1016309360230.500946817305-0.1817355005970.40226808823822.9226699383-2.22587422447-34.0767691077
112.04205207199-1.14659999113-0.2986677020882.177029151890.3029984428461.054937694480.09667053237390.284228947196-0.510870443068-0.295785274652-0.3700329413730.6785233913130.493431745559-0.6847637875950.2305458436970.571485651424-0.104009817835-0.02120232926650.641532211429-0.2143534454680.5909335017942.00046229187-6.04113559586-25.2235346557
121.80416812351-0.375652647672-0.4882905733621.936144284790.5871229272652.17280061192-0.2100020532350.1688651527-0.3659467446880.0125159754190.154173256286-0.03136319735540.4894360347890.01360332569910.06198338181910.476505499526-0.09458954382050.04170499822330.321638844014-0.1327955277070.36353596649716.383336067-7.119867106-22.303470402
131.62777399594-0.09676669070410.1049234690671.473392927650.7609932859681.13578925891-0.0371306065424-0.04519673820690.06278724224240.5546315626310.219389054212-0.2074954646840.2693491767990.53371501152-0.1791724005070.882437469427-0.03074861225310.005798739233280.548422324657-0.1705228851940.47663394073227.457353901492.843521503781.9579681645
140.2568408576990.0643008509629-0.0342492471575-0.0482360475611-0.03041804538360.379471980913-0.0294926447431-0.031655103632-0.05203104551890.3509310286830.52900287952-0.5746924071890.4713654355980.694662575825-0.513204581240.3748268078840.07541400804990.02950560803130.680868624472-0.2637811350690.62655164090343.096345655428.05039124054.1941113896
151.54742538490.716464950673-1.351917491422.005687101030.5240862715122.40277199734-0.4371745380020.326466657922-0.572059007138-0.1905922576220.0468385363941-0.2011036442090.913814877182-0.3893914245340.3166043955580.947901809553-0.136504070030.1686795003070.545917569773-0.1607640829960.5888632422056.1751430765154.931880860969.917418691
163.72957483930.612753271357-0.3823563263663.11034270825-1.629114980634.141707730740.121970080481-0.7367533295620.09355524182550.47817802543-0.164034659745-0.806903072406-0.2588362244110.951220961254-0.02224875699270.651060368685-0.00323321948117-0.05769504198910.7434372368720.0334987976260.59949772209111.120803695666.113231345797.5164873171
172.76405308330.754570841683-0.6432447502252.69403232577-0.03516377386642.9065592071-0.0795311521916-0.812988339773-0.717141528560.816059839375-0.345774110906-0.9058372613160.4156412277111.816052152570.363367181880.9486436795430.150322793196-0.1140449997851.498454134330.4669681691390.9809724177816.180615128455.0070392101108.747664771
180.5222636099010.476162644261-0.6198340573560.997716028104-0.3623878680530.90506421241-0.672553022106-0.0491896886543-0.9171320661680.08423413933780.0738359699647-0.3346576373481.2645847371-0.0492953862551-0.1496014570771.16691616360.1110397585120.3043549749020.4381381301330.1801197927330.925586184303-2.7343734048548.116994503599.1433327362
192.338161308490.423606756204-1.726632685160.9585522175950.308032252222.72238268735-0.271158264120.0638070673734-0.436529633620.0123865705760.217211107576-0.03412568505910.459909176161-0.0381381071050.02360893525140.6895453949650.005437031607920.08658027720780.3935361941770.01592508177940.490546726110.52972684862160.03753365289.6007225412
202.52171645797-1.27181070288-1.637055037640.89617394283-0.2879729758748.074272776770.1866190005770.528712847130.140375475163-0.1528803332720.2265399227010.402018062664-0.916923810252-1.1041547108-0.4740640664390.613573598076-0.06257071271450.01163834527960.641452378123-0.03442120434480.615704311314-7.1853141159563.869304597679.556673285
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 273 )
2X-RAY DIFFRACTION2chain 'A' and (resid 274 through 311 )
3X-RAY DIFFRACTION3chain 'A' and (resid 312 through 438 )
4X-RAY DIFFRACTION4chain 'B' and (resid 1 through 88 )
5X-RAY DIFFRACTION5chain 'B' and (resid 89 through 259 )
6X-RAY DIFFRACTION6chain 'B' and (resid 260 through 437 )
7X-RAY DIFFRACTION7chain 'C' and (resid 1 through 197 )
8X-RAY DIFFRACTION8chain 'C' and (resid 198 through 440 )
9X-RAY DIFFRACTION9chain 'D' and (resid 1 through 88 )
10X-RAY DIFFRACTION10chain 'D' and (resid 89 through 273 )
11X-RAY DIFFRACTION11chain 'D' and (resid 274 through 311 )
12X-RAY DIFFRACTION12chain 'D' and (resid 312 through 441 )
13X-RAY DIFFRACTION13chain 'E' and (resid 6 through 46 )
14X-RAY DIFFRACTION14chain 'E' and (resid 47 through 143 )
15X-RAY DIFFRACTION15chain 'F' and (resid 1 through 66 )
16X-RAY DIFFRACTION16chain 'F' and (resid 67 through 96 )
17X-RAY DIFFRACTION17chain 'F' and (resid 97 through 198 )
18X-RAY DIFFRACTION18chain 'F' and (resid 199 through 283 )
19X-RAY DIFFRACTION19chain 'F' and (resid 284 through 353 )
20X-RAY DIFFRACTION20chain 'F' and (resid 354 through 382 )

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