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- PDB-8b7b: Tubulin - maytansinoid - 6 complex -

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Basic information

Entry
Database: PDB / ID: 8b7b
TitleTubulin - maytansinoid - 6 complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin tyrosine ligase
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE
Function / homology
Function and homology information


tubulin-tyrosine ligase / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / microtubule-based process / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / protein modification process / spindle microtubule ...tubulin-tyrosine ligase / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / microtubule-based process / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / protein modification process / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / microtubule cytoskeleton / neuron projection development / nervous system development / mitotic cell cycle / growth cone / microtubule / neuron projection / protein heterodimerization activity / GTPase activity / GTP binding / Golgi apparatus / ATP binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. ...Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Chem-PX0 / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.25 Å
AuthorsBoiarska, Z. / Perez-Pena, H. / Abel, A.-C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. ...Boiarska, Z. / Perez-Pena, H. / Abel, A.-C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Steinmetz, M.O. / Prota, A.E. / Pieraccini, S. / Passarella, D.
Funding supportEuropean Union, Switzerland, 2items
OrganizationGrant numberCountry
H2020 Marie Curie Actions of the European Commission860070 TubInTrainEuropean Union
Swiss National Science Foundation31003A_166608 Switzerland
CitationJournal: Chemistry / Year: 2023
Title: Maytansinol Functionalization: Towards Useful Probes for Studying Microtubule Dynamics.
Authors: Boiarska, Z. / Perez-Pena, H. / Abel, A.C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Prota, A.E. ...Authors: Boiarska, Z. / Perez-Pena, H. / Abel, A.C. / Marzullo, P. / Alvarez-Bernad, B. / Bonato, F. / Santini, B. / Horvath, D. / Lucena-Agell, D. / Vasile, F. / Sironi, M. / Diaz, J.F. / Prota, A.E. / Pieraccini, S. / Passarella, D.
History
DepositionSep 29, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 23, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,44024
Polymers261,6316
Non-polymers3,80918
Water13,205733
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)104.085, 155.637, 181.924
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Organ: Brain / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Organ: Brain / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043
#4: Protein Tubulin tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8V0Z8P0

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Non-polymers , 9 types, 751 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mg
#7: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#9: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#10: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#11: Chemical ChemComp-PX0 / (~{Z})-11-[[(1~{R},2~{R},3~{S},5~{S},6~{S},16~{E},18~{E},20~{R},21~{S})-11-chloranyl-12,20-dimethoxy-2,5,9,16-tetramethyl-21-oxidanyl-8,23-bis(oxidanylidene)-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1^{10,14}.0^{3,5}]hexacosa-10(26),11,13,16,18-pentaen-6-yl]oxy]-11-oxidanylidene-undec-2-enoic acid


Mass: 761.298 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C39H53ClN2O11 / Feature type: SUBJECT OF INVESTIGATION
#12: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 733 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.8 Å3/Da / Density % sol: 56.6 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 0.999995 Å
DetectorType: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Mar 12, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.999995 Å / Relative weight: 1
ReflectionResolution: 2.25→47.83 Å / Num. obs: 139947 / % possible obs: 99.36 % / Redundancy: 13.7 % / Biso Wilson estimate: 49.7 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.149 / Rpim(I) all: 0.04133 / Rrim(I) all: 0.1546 / Net I/σ(I): 16.76
Reflection shellResolution: 2.25→2.33 Å / Redundancy: 13.3 % / Rmerge(I) obs: 2.913 / Mean I/σ(I) obs: 0.86 / Num. unique obs: 13689 / CC1/2: 0.357 / CC star: 0.725 / Rpim(I) all: 0.8236 / Rrim(I) all: 3.029 / % possible all: 98.84

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Processing

Software
NameVersionClassification
PHENIX1.20_4459refinement
XDSdata reduction
PDB_EXTRACT3.28data extraction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS
Starting model: 5LXT

5lxt


Resolution: 2.25→47.83 Å / SU ML: 0.3245 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.3769
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2243 6996 5 %
Rwork0.1837 132933 -
obs0.1858 139929 99.31 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 68.23 Å2
Refinement stepCycle: LAST / Resolution: 2.25→47.83 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17263 0 232 733 18228
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002117970
X-RAY DIFFRACTIONf_angle_d0.489524377
X-RAY DIFFRACTIONf_chiral_restr0.042650
X-RAY DIFFRACTIONf_plane_restr0.00323160
X-RAY DIFFRACTIONf_dihedral_angle_d12.06046692
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.25-2.270.40842240.35734245X-RAY DIFFRACTION96.44
2.27-2.30.3342310.32184404X-RAY DIFFRACTION99.14
2.3-2.330.31112290.3024351X-RAY DIFFRACTION98.73
2.33-2.360.32412300.29184366X-RAY DIFFRACTION98.92
2.36-2.390.31812300.29154363X-RAY DIFFRACTION99.22
2.39-2.420.34642300.29124360X-RAY DIFFRACTION98.33
2.42-2.450.30432300.26854385X-RAY DIFFRACTION99.94
2.45-2.490.30852310.26294385X-RAY DIFFRACTION98.49
2.49-2.530.31662300.25684376X-RAY DIFFRACTION99.22
2.53-2.570.28392320.23634404X-RAY DIFFRACTION99.42
2.57-2.620.27292300.22964359X-RAY DIFFRACTION98.82
2.62-2.660.29532320.23144421X-RAY DIFFRACTION99.25
2.66-2.720.29192320.22744398X-RAY DIFFRACTION99.68
2.72-2.770.28942320.22534401X-RAY DIFFRACTION99.1
2.77-2.830.28112320.21344421X-RAY DIFFRACTION99.19
2.83-2.90.26792310.21664377X-RAY DIFFRACTION99.27
2.9-2.970.22992330.21554436X-RAY DIFFRACTION99.62
2.97-3.050.23252330.19184423X-RAY DIFFRACTION99.74
3.05-3.140.23582340.18854454X-RAY DIFFRACTION99.51
3.14-3.240.25082330.19164422X-RAY DIFFRACTION99.55
3.24-3.360.24172340.18794436X-RAY DIFFRACTION99.38
3.36-3.490.21352330.17984432X-RAY DIFFRACTION99.66
3.49-3.650.20612350.174469X-RAY DIFFRACTION99.79
3.65-3.840.21412350.15954470X-RAY DIFFRACTION99.81
3.84-4.080.18412360.15074482X-RAY DIFFRACTION99.75
4.08-4.40.17852360.14214485X-RAY DIFFRACTION99.83
4.4-4.840.1822380.12994514X-RAY DIFFRACTION99.89
4.84-5.540.1892400.15224555X-RAY DIFFRACTION99.92
5.54-6.970.22652410.17744584X-RAY DIFFRACTION99.92
6.97-47.830.18132490.16814755X-RAY DIFFRACTION99.7
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.814582040409-0.762053780864-0.6006039125911.5060202529-0.1432808503011.019169926780.02865470486870.1567859558350.294270887511-0.5162805832750.214455275839-0.129106472098-0.925982105510.1321979679957.36507086433E-50.922965390764-0.1737928906990.1039117815260.617969519607-0.1213989785170.62471215556628.446298858895.358708128452.9193122129
20.426548789105-0.02592723970750.6923224273190.6473781591320.7273139485031.73226863799-0.1153184979580.4255369892250.100984870902-0.834085011390.528745964506-0.568027832662-0.8980976264250.757090180726-0.001617227590170.773513785604-0.2075056314450.1982054820550.783561167323-0.2309888341290.73245444363435.067936964582.688425951145.8035295782
30.467922506693-0.825881939719-0.6679251235062.592552639650.349997145722.042215296850.108420364573-0.1155516404570.0009329642166090.2229564106950.242301293276-0.382274745126-0.1219500336680.7086523018770.002630540187960.455441106114-0.03503296018510.03082975036180.670003120009-0.2549229063480.57039084462632.820915158376.531744090157.8136472848
40.834365910724-0.796001600799-0.507362596572.130330148470.1749394142062.52862250620.04568984837270.0648118539217-0.02018576229950.08217993158720.1021191801670.00864314430382-0.2868025754290.0940774566434-0.0001026687118360.5488326466820.01809096337160.07341971162290.479786626758-0.07821318346530.53985679517219.787603176584.48441952763.4103570676
51.456220574661.271119633170.2105440361882.556505408881.191257128282.60100627090.0543217081479-0.120939470832-0.01641676013680.479578094512-0.06103896888420.451640379023-0.0573824666418-0.2273151170446.59707684016E-50.6015002529220.05006236600690.08217446687340.53869540124-0.110860169240.54847359704213.724431850282.971538870.9613222059
61.036356792010.327257938270.202807860440.2499922748810.2621087723060.5766511783380.00218968243362-0.3093000723220.152479871350.450032102349-0.002799554554120.105356537301-0.225614296102-0.00614341525597-1.3566778852E-50.8064748135920.04976122111050.1054964130040.589979176745-0.1221673490060.5961473128817.013508896486.341997230380.3977278213
70.5241044800110.2936068296990.2861447779432.589301724591.47213431722.505651157550.00264424301799-0.121524403837-0.003860652580240.4237914005050.1558348351820.02873453869210.008622272139320.2637863201770.0005160169466240.477050466227-0.000789470700290.03106979627740.495588399261-0.105107991810.50250622319321.82666815975.327814551267.1047171126
80.965342283011-0.105360413857-0.4756411183691.870100651831.552801597841.45738998781-0.390648019809-0.15734024197-0.4996391303141.481506191720.2145416886930.01942434135041.47764183830.675386833102-0.004522189789990.8722519759720.176344138103-0.1406350424430.658413839361-0.1351786005240.68717837548431.738370413962.553702217169.4565591722
92.08524192708-0.974447858785-0.17981693921.32663515099-0.7927689504971.774300330450.03492691162890.2067173000650.558198823496-0.439411519513-0.03519234538680.130233300126-0.897507383866-0.14111175942-0.000131457874940.6830414554280.05683340086370.01909382726830.564382049411-0.02803309479520.6981701653916.191819592869.432073625819.3707089986
101.7455070164-0.333845076744-0.2740624511332.301430421091.390470158873.384239057240.02944755465170.09824860853640.125847093916-0.137895759237-0.03577573181670.0658086231512-0.2315716554080.0360425526896-0.01661111650950.3418446118130.0149242925670.02162739159390.46350802523-0.09365778859850.50389497568920.129962428355.351228665725.3647915088
110.846346352938-0.4637094073860.05484301916991.898259427981.440539250772.70598114292-0.134591270466-0.1937443623930.04190836842710.357727721425-0.06783190707460.2050375314170.195297803677-0.3633798442-0.006692035478810.420817333665-0.02856479631260.04370339441250.554486254813-0.09163422954710.54647338659411.980577300351.572642653238.4534976751
120.113181648459-0.103960713651-0.6496239467330.09589720386490.5966621960433.76464513532-0.088195808093-0.0109433423488-0.138578245484-0.0299310624443-0.08654363987460.05932610926910.0670868825437-0.001057175965450.004642407221011.28671461149-0.381189647006-0.1717726468731.161017806980.1267509727550.87026538431918.394357644945.573215592556.6330493135
131.19272930655-0.2719359760270.08734036327552.304797842890.3944023032362.00486658891-0.0745200196810.1862280757680.137221036443-0.3163008046790.147420535613-0.0436714530536-0.16555304560.1238232141661.37087089903E-50.399795188548-0.08734989067010.04664413307220.485579105014-0.03813227838980.46773775174720.222433153432.3614421183-11.9415676211
141.19978268291-0.453550883106-0.08256461999971.826781653561.106044346761.94500494982-0.0635120135514-0.03109093047990.04358265809780.101002845814-0.04060778254710.1493797822860.103765649249-0.2306430889852.90687000127E-50.376381044688-0.08394231946620.04831214625640.433553086175-0.02742307639110.4739901637627.5821022239625.46304591563.18664364836
151.63840087862-0.2069845913670.6091405948930.892468882856-0.5179875202281.35621553334-0.1932804516480.7673509123930.230219875671-0.6893354606040.3247575439980.001860097803560.105517027232-0.5964470128480.00262069319560.923800968188-0.1698383805350.03789556024821.12413835834-0.07866701839810.56855498205917.13805518798.56797311261-43.8894778012
161.87018834932-0.148060188444-0.6935302193841.632928789760.3559037470592.75474752515-0.2434997550340.528671842676-0.297523507161-0.307995548930.139067520234-0.01766889683390.547614410918-0.232689783607-0.01717392360310.811588807548-0.1336872480170.07434965947840.691699032228-0.2265916340370.58167891574718.2234052069-5.43555698826-28.5392764041
170.621738793150.15114131245-0.09085009172860.5388776497430.5014785344090.521483012831-0.162303498118-0.247263934050.1365587178760.668280507380.146208766121-0.5198994646280.17337253420.764823162-9.27018505398E-51.083817117670.00709301325284-0.04590313538640.869430935513-0.148904072370.76197592614627.244494111291.254248232282.4726558051
180.399624782051-0.35059846377-0.7100666754820.03812546440460.06034939820950.470950189676-0.0263837354016-0.0731414574246-0.003797365014430.4208911956810.357847451105-0.4596670383580.6672419136610.5574112191170.06498240230270.6053896974780.09955153183770.0297981097350.860507006954-0.2624430650630.79716498359442.773069204526.85660654354.11997268292
191.488900635290.654829742196-1.246943769960.8870918862380.05888122319341.49749581857-0.3742126666790.366551578568-0.432522793419-0.3261558220380.146127552851-0.02790854061781.17836720212-0.313023855339-4.3727779553E-51.11836669815-0.1019878355590.117070925710.678728638387-0.1358666777960.7932786836756.0216199432353.604731071369.7753616145
200.392005336512-0.0292842410132-0.2354162506730.356744863396-0.1901641126260.3459846010190.102326104683-0.751411102923-0.143729496490.774419820165-0.562645261994-0.876544923696-0.416457918491.79191808445-9.36497010901E-51.189833766430.0196748985839-0.1546325428141.487658007720.2443993944590.9917370913115.88364876560.2616266356104.948654165
211.157540648680.992684693476-1.390764488561.36235320324-0.4551454162732.20909005404-0.466867028847-0.389828218814-0.7639249043380.4753021946420.0800855017544-0.2281758316460.9160412575150.372997551025-0.01785963243761.398618608390.1876015380130.2636642602220.7214929218250.263563003791.047601209280.90069083541646.9496412604101.129570784
222.30479101192-0.0592599814932-1.363109604080.8296040642680.03639150005771.70760987388-0.255387667598-0.0160917129648-0.4995696811870.1928425372480.2344308296640.06855694426010.525794505484-0.1915517001675.14214165303E-50.9324451213860.02620094249540.1193101861940.5941809848030.04255138494750.740861150348-0.96416311385758.755971121587.5375949077
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 79 )AA1 - 791 - 79
22chain 'A' and (resid 80 through 127 )AA80 - 12780 - 127
33chain 'A' and (resid 128 through 197 )AA128 - 197128 - 197
44chain 'A' and (resid 198 through 259 )AA198 - 259198 - 259
55chain 'A' and (resid 260 through 306 )AA260 - 306260 - 306
66chain 'A' and (resid 307 through 336 )AA307 - 336307 - 336
77chain 'A' and (resid 337 through 414 )AA337 - 414337 - 414
88chain 'A' and (resid 415 through 438 )AA415 - 438415 - 438
99chain 'B' and (resid 1 through 88 )BF1 - 881 - 86
1010chain 'B' and (resid 89 through 259 )BF89 - 25987 - 257
1111chain 'B' and (resid 260 through 437 )BF260 - 437258 - 423
1212chain 'B' and (resid 438 through 439 )BF438 - 439424 - 425
1313chain 'C' and (resid 1 through 197 )CL1 - 1971 - 197
1414chain 'C' and (resid 198 through 440 )CL198 - 440198 - 440
1515chain 'D' and (resid 1 through 88 )DQ1 - 881 - 86
1616chain 'D' and (resid 89 through 441 )DQ89 - 44187 - 426
1717chain 'E' and (resid 6 through 46 )EU6 - 461 - 26
1818chain 'E' and (resid 47 through 143 )EU47 - 14327 - 123
1919chain 'F' and (resid 1 through 66 )FV1 - 661 - 66
2020chain 'F' and (resid 67 through 165 )FV67 - 16567 - 132
2121chain 'F' and (resid 166 through 283 )FV166 - 283133 - 241
2222chain 'F' and (resid 284 through 380 )FV284 - 380242 - 328

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