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Open data
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Basic information
Entry | Database: PDB / ID: 8awm | ||||||||||||
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Title | RVFV GnH with Fab268 bound | ||||||||||||
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![]() | VIRAL PROTEIN / RVFV / Gn / Fab268 | ||||||||||||
Function / homology | ![]() | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Hulswit, R.J.G. / Bowden, T.A. / Stass, R. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Multifunctional human monoclonal antibody combination mediates protection against Rift Valley fever virus at low doses. Authors: Chapman, N.S. / Hulswit, R.J.G. / Westover, J.L.B. / Stass, R. / Paesen, G.C. / Binshtein, E. / Reidy, J.X. / Engdahl, T.B. / Handal, L.S. / Flores, A. / Gowen, B.B. / Bowden, T.A. / Crowe Jr., J.E. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.8 KB | Display | ![]() |
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PDB format | ![]() | 117.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.8 KB | Display | ![]() |
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Full document | ![]() | 451.5 KB | Display | |
Data in XML | ![]() | 26.6 KB | Display | |
Data in CIF | ![]() | 35.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8awlC ![]() 6f8pS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Antibody | Mass: 23633.486 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Antibody | Mass: 22768.014 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Protein | Mass: 36075.066 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.25 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / Details: 0.1 M bis-tris pH 5.5, 25% w/v PEG 3,350 / Temp details: room temperature |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Jul 16, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→61.57 Å / Num. obs: 10540 / % possible obs: 100 % / Redundancy: 13 % / CC1/2: 0.992 / Rmerge(I) obs: 0.56 / Net I/σ(I): 2.7 |
Reflection shell | Resolution: 3.5→3.56 Å / Rmerge(I) obs: 2.004 / Num. unique obs: 558 / CC1/2: 0.789 / Rpim(I) all: 0.569 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6f8p Resolution: 3.5→61.57 Å / SU ML: 0.51 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 28.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 158.68 Å2 / Biso mean: 80.8076 Å2 / Biso min: 42.64 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.5→61.57 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 4
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