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- PDB-7zlm: Crystal structure of SOCS2:ElonginB:ElonginC in complex with comp... -

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Open data

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Basic information

Entry

Database: PDB / ID: 7zlm

Title

Crystal structure of SOCS2:ElonginB:ElonginC in complex with compound MN551

Components

Elongin-B
Elongin-C
Suppressor of cytokine signaling 2

Keywords

LIGASE / E3 ligase / suppressor of cytokine signaling

Function / homology

Function and homology information

JAK pathway signal transduction adaptor activity / negative regulation of receptor signaling pathway via JAK-STAT / 1-phosphatidylinositol-3-kinase regulator activity / target-directed miRNA degradation / elongin complex / VCB complex / phosphatidylinositol 3-kinase complex / growth hormone receptor binding / Cul5-RING ubiquitin ligase complex / growth hormone receptor signaling pathway ...JAK pathway signal transduction adaptor activity / negative regulation of receptor signaling pathway via JAK-STAT / 1-phosphatidylinositol-3-kinase regulator activity / target-directed miRNA degradation / elongin complex / VCB complex / phosphatidylinositol 3-kinase complex / growth hormone receptor binding / Cul5-RING ubiquitin ligase complex / growth hormone receptor signaling pathway / Cul2-RING ubiquitin ligase complex / negative regulation of multicellular organism growth / cell surface receptor signaling pathway via JAK-STAT / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / phosphatidylinositol phosphate biosynthetic process / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / regulation of signal transduction / Tat-mediated elongation of the HIV-1 transcript / mammary gland alveolus development / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / Growth hormone receptor signaling / cellular response to hormone stimulus / RNA Polymerase II Pre-transcription Events / Negative regulation of FLT3 / lactation / positive regulation of neuron differentiation / Interleukin-7 signaling / transcription corepressor binding / transcription elongation by RNA polymerase II / regulation of cell growth / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / Vif-mediated degradation of APOBEC3G / insulin-like growth factor receptor binding / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Inactivation of CSF3 (G-CSF) signaling / Evasion by RSV of host interferon responses / Regulation of expression of SLITs and ROBOs / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / response to estradiol / protein-macromolecule adaptor activity / Neddylation / ubiquitin-dependent protein catabolic process / protein-containing complex assembly / protein ubiquitination / intracellular signal transduction / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / nucleoplasm / cytoplasm / cytosol
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.79 Å

Authors

Ramachandran, S. / Ciulli, A. / Makukhin, N.

Funding support

European Union,

Switzerland, 2items

Citation

Journal: Nat Commun / Year: 2023
Title: Structure-based design of a phosphotyrosine-masked covalent ligand targeting the E3 ligase SOCS2.
Authors: Ramachandran, S. / Makukhin, N. / Haubrich, K. / Nagala, M. / Forrester, B. / Lynch, D.M. / Casement, R. / Testa, A. / Bruno, E. / Gitto, R. / Ciulli, A.

History

Deposition	Apr 15, 2022	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Apr 26, 2023	Provider: repository / Type: Initial release
Revision 1.1	Oct 11, 2023	Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / citation / Item: _citation.pdbx_database_id_DOI / _citation.year
Revision 1.2	Oct 25, 2023	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.3	May 1, 2024	Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

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Downloads & links

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Download

PDBx/mmCIF format	7zlm.cif.gz	702.9 KB	Display	PDBx/mmCIF format
PDB format	pdb7zlm.ent.gz	479.7 KB	Display	PDB format
PDBx/mmJSON format	7zlm.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7zlm_validation.pdf.gz	1.5 MB	Display	wwPDB validaton report
Full document	7zlm_full_validation.pdf.gz	1.5 MB	Display
Data in XML	7zlm_validation.xml.gz	58.2 KB	Display
Data in CIF	7zlm_validation.cif.gz	84.5 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/zl/7zlm ftp://data.pdbj.org/pub/pdb/validation_reports/zl/7zlm	HTTPS FTP

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Related structure data

Related structure data	7zlnC 7zloC 7zlpC 7zlrC 7zlsC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#240 - Dec 2019 Hypoxia-Inducible Factors similarity (20) #60 - Dec 2004 Ubiquitin similarity (6) #110 - Feb 2009 Auxin and TIR1 Ubiquitin Ligase similarity (5) #218 - Feb 2018 EPSP Synthase and Weedkillers similarity (5) #52 - Apr 2004 Growth Hormone similarity (1) #67 - Jul 2005 TATA-Binding Protein similarity (1) #122 - Feb 2010 Enhanceosome similarity (2) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #234 - Jun 2019 MDM2 and Cancer similarity (2) #213 - Sep 2017 Sirtuins similarity (1) #126 - Jun 2010 Epidermal Growth Factor similarity (1) #221 - May 2018 Human Papillomavirus and Vaccines similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#240 - Dec 2019
Hypoxia-Inducible Factors
similarity (20)
#60 - Dec 2004
Ubiquitin
similarity (6)
#110 - Feb 2009
Auxin and TIR1 Ubiquitin Ligase
similarity (5)
#218 - Feb 2018
EPSP Synthase and Weedkillers
similarity (5)
#52 - Apr 2004
Growth Hormone
similarity (1)
#67 - Jul 2005
TATA-Binding Protein
similarity (1)
#122 - Feb 2010
Enhanceosome
similarity (2)
#31 - Jul 2002
p53 Tumor Suppressor
similarity (1)
#234 - Jun 2019
MDM2 and Cancer
similarity (2)
#213 - Sep 2017
Sirtuins
similarity (1)
#126 - Jun 2010
Epidermal Growth Factor
similarity (1)
#221 - May 2018
Human Papillomavirus and Vaccines
similarity (1)

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Assembly

Deposited unit

A: Suppressor of cytokine signaling 2

B: Elongin-B

C: Elongin-C

D: Suppressor of cytokine signaling 2

E: Elongin-B

F: Elongin-C

G: Suppressor of cytokine signaling 2

H: Elongin-B

I: Elongin-C

J: Suppressor of cytokine signaling 2

K: Elongin-B

L: Elongin-C

hetero molecules

176 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

A: Suppressor of cytokine signaling 2

B: Elongin-B

C: Elongin-C

hetero molecules

defined by author&software
Evidence: gel filtration
44 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

D: Suppressor of cytokine signaling 2

E: Elongin-B

F: Elongin-C

hetero molecules

defined by author&software
44 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

G: Suppressor of cytokine signaling 2

H: Elongin-B

I: Elongin-C

hetero molecules

defined by author&software
44 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

J: Suppressor of cytokine signaling 2

K: Elongin-B

L: Elongin-C

hetero molecules

defined by author&software
44 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	59.785, 172.879, 185.809
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁
Space group name Hall	P2ac2ab
Symmetry operation	#1: x,y,z #2: x+1/2,-y+1/2,-z #3: -x,y+1/2,-z+1/2 #4: -x+1/2,-y,z+1/2

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Components

#1: Protein	Suppressor of cytokine signaling 2 / SOCS-2 / Cytokine-inducible SH2 protein 2 / CIS-2 / STAT-induced STAT inhibitor 2 / SSI-2 Mass: 19273.268 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SOCS2, CIS2, SSI2, STATI2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O14508
#2: Protein	Elongin-B / EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / ...EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / Transcription elongation factor B polypeptide 2 Mass: 13147.781 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ELOB, TCEB2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q15370
#3: Protein	Elongin-C / EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / ...EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / Transcription elongation factor B polypeptide 1 Mass: 10974.616 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ELOC, TCEB1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q15369
#4: Chemical	ChemComp-JIH / [4-[(2~{S})-3-[[3-(2-chloranylethanoylamino)phenyl]methylamino]-2-[2-(4-fluorophenyl)ethanoylamino]-3-oxidanylidene-propyl]phenyl] dihydrogen phosphate Mass: 577.926 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂₆H₂₆ClFN₃O₇P / Feature type: SUBJECT OF INVESTIGATION
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 919 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y

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Experimental details

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.77 Å³/Da / Density % sol: 55.53 %
Crystal grow	Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Bis-Tris propane 6.5, 20 % w/v PEG 3350 with seeding

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-1 / Wavelength: 0.966 Å
Detector	Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Nov 26, 2020
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.966 Å / Relative weight: 1
Reflection	Resolution: 1.79→78.37 Å / Num. obs: 181660 / % possible obs: 99.3 % / Redundancy: 4.5 % / B_iso Wilson estimate: 29.52 Å² / CC_1/2: 0.991 / Net I/σ(I): 7
Reflection shell	Resolution: 1.79→1.82 Å / R_merge(I) obs: 1.605 / Num. unique obs: 8234 / CC_1/2: 0.351

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Processing

Software

Name	Version	Classification
Coot		model building
PHENIX	1.19.2_4158	refinement
xia2		data reduction
xia2		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: SBC

Resolution: 1.79→78.37 Å / SU ML: 0.2662 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 21.4531
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.217	8980	4.95 %
R_work	0.1893	172451	-
obs	0.1907	181431	99.21 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 37.61 Å²

Refinement step

Cycle: LAST / Resolution: 1.79→78.37 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10738	0	152	919	11809

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0076	11175
X-RAY DIFFRACTION	f_angle_d	0.9366	15155
X-RAY DIFFRACTION	f_chiral_restr	0.059	1729
X-RAY DIFFRACTION	f_plane_restr	0.0071	1911
X-RAY DIFFRACTION	f_dihedral_angle_d	9.6184	1610

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.79-1.81	0.4518	245	0.4375	4976	X-RAY DIFFRACTION	86.18
1.81-1.83	0.4717	295	0.4081	5670	X-RAY DIFFRACTION	99.43
1.83-1.85	0.3825	314	0.3624	5651	X-RAY DIFFRACTION	99.67
1.85-1.87	0.3446	306	0.3153	5767	X-RAY DIFFRACTION	99.75
1.87-1.9	0.305	279	0.2808	5690	X-RAY DIFFRACTION	99.62
1.9-1.92	0.297	295	0.2576	5742	X-RAY DIFFRACTION	99.52
1.92-1.95	0.248	293	0.2392	5724	X-RAY DIFFRACTION	99.59
1.95-1.98	0.2735	302	0.2224	5703	X-RAY DIFFRACTION	99.6
1.98-2.01	0.2352	311	0.2183	5687	X-RAY DIFFRACTION	99.5
2.01-2.05	0.2521	294	0.2069	5727	X-RAY DIFFRACTION	99.69
2.05-2.08	0.2543	296	0.2071	5754	X-RAY DIFFRACTION	99.74
2.08-2.12	0.2249	291	0.2106	5729	X-RAY DIFFRACTION	99.73
2.12-2.16	0.2605	277	0.2097	5749	X-RAY DIFFRACTION	99.65
2.16-2.2	0.2334	312	0.2128	5743	X-RAY DIFFRACTION	99.65
2.2-2.25	0.2337	309	0.2159	5712	X-RAY DIFFRACTION	99.73
2.25-2.3	0.2454	338	0.2011	5742	X-RAY DIFFRACTION	99.66
2.3-2.36	0.2369	316	0.1876	5699	X-RAY DIFFRACTION	99.8
2.36-2.42	0.2106	321	0.1912	5729	X-RAY DIFFRACTION	99.74
2.42-2.5	0.2304	293	0.1874	5825	X-RAY DIFFRACTION	99.8
2.5-2.58	0.2277	285	0.1887	5754	X-RAY DIFFRACTION	99.72
2.58-2.67	0.2096	298	0.1853	5745	X-RAY DIFFRACTION	99.46
2.67-2.78	0.2148	308	0.1869	5782	X-RAY DIFFRACTION	99.74
2.78-2.9	0.2265	258	0.1966	5855	X-RAY DIFFRACTION	99.77
2.9-3.05	0.2203	295	0.1943	5795	X-RAY DIFFRACTION	99.89
3.05-3.25	0.2263	297	0.1808	5829	X-RAY DIFFRACTION	99.84
3.25-3.5	0.1932	290	0.1738	5867	X-RAY DIFFRACTION	99.84
3.5-3.85	0.1997	287	0.1612	5860	X-RAY DIFFRACTION	99.64
3.85-4.41	0.1518	323	0.1417	5835	X-RAY DIFFRACTION	99.26
4.41-5.55	0.1876	340	0.1608	5917	X-RAY DIFFRACTION	99.41
5.55-78.37	0.2103	312	0.1877	6193	X-RAY DIFFRACTION	99.57

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.33721730158	4.89102789002	-5.56231555434	4.45920873452	-5.34712912243	6.77481507014	0.0661300325305	0.0589109057127	0.146327227747	0.039872455319	-0.0592145914142	0.433335602126	-0.0280255282854	-0.376604846169	0.0255729013894	0.2590941029	-0.0198883499438	-0.00785383872659	0.29514534046	-0.0361814932902	0.253815552721	-19.4833587541	-38.0098152081	-15.770183665
2	3.02018441187	-0.95875152217	0.345285678672	3.85342708034	-0.117522359926	4.52528761766	0.153511496467	-0.204944978282	-0.010606559603	0.20542362139	-0.024279811051	-0.369534277732	0.0559367930474	0.19194238211	-0.102386223947	0.144705955453	-0.0164205557991	-0.0307914506555	0.15320766062	-0.00628563120685	0.126152676004	-2.31072468458	-30.1445765071	-11.5363928691
3	3.27407297851	1.28746934954	3.8880078091	3.84219337484	2.86447792655	5.16573348908	0.169700497531	-0.93811706463	-0.296133647372	0.416860025131	-0.191039987485	0.032940637993	0.0292380757906	-0.33162476885	-0.0343629083804	0.232599616523	-0.00233888862695	-0.0244627420351	0.21931915112	0.0312962596048	0.164439133927	-9.60128932317	-30.9297342158	-5.4043408999
4	3.1088297633	-3.98636972166	1.67436886561	5.6651319146	-0.697562471023	4.82635385822	-0.0796773052869	-1.24595269711	0.138449183477	0.67291859204	-0.25045391276	0.120119713741	-0.00885679367429	-0.784851332975	0.316190854118	0.257208955415	-0.0186817071637	0.0422737457253	0.419181510125	-0.0166824748325	0.201618577104	-16.7365257704	-29.9737166959	-2.2647016716
5	6.48884579671	0.966986354027	-0.659104438745	6.79269135638	0.194400838219	4.87525653163	0.0171335419976	-0.324543504573	0.509979255522	0.385485695478	-0.0701080347383	0.460182660764	-0.338725582639	-0.294193068028	0.03658537482	0.23353131188	0.0698581518864	-0.00575779399363	0.282909569633	-0.0374660120612	0.216973622889	-17.8492739301	-23.5281281902	-10.7578317198
6	4.10154521726	0.0126527212963	0.575216034386	7.41299543305	4.75864758906	3.24072472524	0.0263855298788	-0.125544198649	0.115604361086	0.476331411893	0.0759119323563	0.00205154426532	0.180178990658	0.167065459737	-0.12458917467	0.270447380024	-0.00339302340302	0.0295851358466	0.184202643277	0.0624923743095	0.201014743071	-6.22469233564	-23.9622867842	-18.9391402021
7	6.23295317799	-2.4049930667	-1.29091961773	3.80873067471	-0.133611641642	2.89645527503	-0.0833128286725	0.335035966823	-0.269508911341	0.030455873878	-0.0293466534964	0.278771530379	0.0887241123205	-0.467641546289	0.116442918285	0.183400916811	-0.0146497942887	0.00725584136655	0.288927353158	0.00173455435395	0.150694478796	-20.9126916585	-25.4634405553	-30.1830524467
8	3.99042947112	-0.188499479734	-0.366590009382	3.71471529171	-0.638670266664	4.31025259971	-0.0429808432226	-0.205118339972	0.0107403673652	0.133045841845	0.0752814599671	-0.218945155481	0.0765641497158	0.0927239084622	-0.0295788981212	0.153486487876	0.0192017573624	-0.000658306095031	0.186955690949	-0.0367835285202	0.156400826255	6.04891810606	-19.7549927621	-46.7284247755
9	7.46229159468	1.57841885262	0.81076960189	7.27673582052	3.43772679302	7.28963602966	-0.334942603609	0.17027921971	-0.593889526909	0.198073110417	0.199444344814	-0.658727989914	0.98152189469	0.989199467892	0.102340353365	0.260881023745	0.0927735426124	0.115131563857	0.278349893953	0.0563459350145	0.324919724394	12.5739945202	-22.1795827665	-54.319180713
10	4.20081699917	-0.445709600288	-0.67977742893	5.47073054229	1.37217450019	7.15162421141	-0.164020549474	0.260044672493	0.0773374882199	-0.387927313796	0.0182535686377	-0.444077698877	0.0947563810605	0.616116183991	0.120021472532	0.212788946492	0.00961295592083	0.0413263280197	0.284152780948	0.0304661103316	0.305351486303	10.9504027334	-16.5140002551	-59.3730945322
11	2.46855883171	-2.83916829358	-0.511503567048	6.0586110473	-1.84095250114	2.36350948979	-0.133902963952	-0.472846518458	1.15100228008	0.0796400333574	-0.0589438485977	-0.672349923431	-1.11178952678	0.518416137346	0.281866092561	0.41348612101	-0.0556161206067	-0.0540303873672	0.431928140389	-0.00528594900126	0.476856116412	12.6280074099	-8.75191408863	-49.8467094085
12	4.71866860623	1.29168698612	-1.0793580822	3.69117688556	-2.13413336881	8.5562652492	0.0234242489048	-0.3858192726	0.295785809198	0.55750220847	-0.387861746527	-0.0712791361231	-1.23486379629	0.416745283109	0.247307404246	0.340409653495	0.0102021244461	-0.0966712219361	0.28549193451	-0.0687027665969	0.293867278045	11.2573032851	-14.5316418405	-39.1929468435
13	2.70358000433	0.537231035679	-0.859826532394	2.89452585374	-3.34629052094	6.83845903423	0.00555415969405	-0.0588476651488	0.168262127762	0.184508700878	0.0599837005659	-0.0846547260034	-0.339022634533	-0.305415648771	-0.142983446221	0.162102191927	0.00577374720652	-0.00689978916943	0.187552247801	-0.0183668171198	0.208825382436	3.65421169727	-16.5835280624	-47.1591304262
14	5.01203533591	-3.71900140774	-4.55224060201	3.71014516321	1.88245763017	6.55070932942	0.313487039823	2.08533672971	0.811533768292	-1.37558525528	0.0616253992541	0.152912880404	-1.06026007545	-1.63097373137	-0.325506304973	0.428493462568	0.0292784226121	0.0503924885411	0.541425277715	0.082318654761	0.345312501319	9.39469515761	-11.0663434943	-68.7244566739
15	9.54063695477	3.1772086715	-7.6815254511	2.1984684072	-2.81493669145	6.17964832755	-0.0417502697461	1.31024063358	0.253079093395	-0.106552932911	0.195334442016	0.178972501048	-0.0209761040362	-0.866419105027	-0.318407134365	0.221246774062	0.0510879767061	-0.00412618939042	0.326236552316	0.0574724628962	0.275513253675	-1.49272911106	-11.1695821382	-55.6892135839
16	3.04528464522	-5.18712909926	-4.07419066108	8.85985795322	6.97451029981	5.49591187908	0.906053413304	0.338638344497	1.44410261436	0.206679132826	0.202453483643	0.943174606161	-1.45141601506	-1.85055307035	-0.880034084539	0.342655624077	0.14571784674	0.130205710816	0.501083395419	0.0795797563757	0.415359991415	-19.8979610198	-12.127229809	-42.0053606375
17	2.25352132358	-3.41100549398	-3.4803840768	7.69890429924	6.90384152618	6.42987139765	0.182712333463	0.160816513334	0.713589468276	-0.286391284677	0.341368477855	1.43003256478	-1.26740421665	-1.87407711805	-0.200351545196	0.385023061544	0.290601879176	0.168067507082	0.778964528227	0.194796826488	0.777745532429	-24.6358844605	-12.831218616	-33.0471109944
18	2.73933833497	-1.6273204678	4.40246557548	8.77502778951	-1.77579962512	7.25767861739	-0.0932439766375	0.864175617458	-0.341019725392	-0.151932389982	-0.0870651629526	-0.453524237523	0.616126112308	0.234790768267	0.127629699429	0.290117820686	0.00112429571908	0.0262438619824	0.288012232994	0.00411833581522	0.181301193848	-0.710927718137	-30.234690797	-52.4640623009
19	5.84938822329	-0.600056795532	0.0900854013948	4.01646476868	-1.62468098618	4.74695333653	-0.126345975186	0.432954311193	-0.479866698823	-0.195325503187	0.00450303957893	0.00660791631922	0.334713061582	-0.117438988115	0.0956187739375	0.202798487293	-0.0409919256298	0.0230344421481	0.224557060088	-0.0829692094874	0.162351958122	-5.14075081696	-29.7670069632	-48.5070775004
20	5.6551745642	3.04851576609	-0.300068404088	5.90228095612	3.42313996422	5.36028602378	-0.0500244037302	0.729689074522	0.180937336316	-0.434446527463	-0.00336752463043	0.190583247826	0.496061252005	-1.00322591681	0.0433785826878	0.281884078704	-0.112167497582	-0.040957634806	0.393405411987	0.00297804011751	0.171746819412	-12.6416617923	-29.2226936817	-52.8658742071
21	5.73034861487	-3.02678033182	1.28737324465	6.10776627834	-1.77478640477	3.81174010763	-0.15247177969	-0.0965940777264	-0.0126357352343	0.137353176225	0.131378618026	-0.176094899618	0.0756100386486	-0.0737311463499	0.052369844789	0.186715981032	0.00329301517192	0.0094827210812	0.231528943381	-0.0163887679649	0.134277661117	-4.31450409715	-25.2868165242	-39.7018389088
22	4.69751354633	0.931241354151	2.88209187977	4.42276708629	-1.02966360262	8.02000993875	0.356418353179	-0.658451580459	-0.263080576214	0.337642661191	-0.200406129788	-0.458432226706	0.646781374109	0.300292870169	-0.047704857008	0.284951571949	0.000498018330006	-0.0203103380973	0.296744478495	0.0532303900279	0.24359119456	-1.07508097509	-25.4205687145	-29.769057239
23	3.99114401742	-0.647334093745	-0.709880194616	8.18004383286	2.74645772709	5.17612787249	0.107999703394	0.444107939555	0.04293067275	0.116295924379	-0.173451137246	0.175341469207	0.1572959248	-0.637924626536	0.0894638642651	0.169289832489	-0.0242726456751	0.00928748605201	0.371324785515	0.0152227210274	0.133602104487	-15.892745242	-25.6007263835	-39.8490711367
24	9.16909356409	-3.91074648001	-5.60834030354	3.9785168164	3.78672107023	4.52855816058	0.142124164426	0.223125928898	-0.298290920983	-0.206504658084	-0.152024470867	0.189469745631	-0.232928839029	-0.519438369126	-0.0756723603949	0.380480483899	-0.0101610472736	0.0212704391486	0.263134921102	0.0601788923806	0.275284593576	-5.48653140036	28.4876363519	0.296772164766
25	4.39394528472	2.18711651764	0.126259907629	6.34139313665	-0.773578749922	2.95562237951	0.253268383155	-0.221389954229	0.00865411082798	0.138341396721	-0.253540110992	-0.392829608133	-0.222834884406	0.254301526427	-0.0159662107436	0.278517012805	-0.0351223613919	0.0428511559049	0.139427687456	-0.011246065282	0.248632086764	10.705843729	33.2838607193	-8.51410680097
26	6.49121317882	0.100259908924	-0.74585893786	2.74096588931	0.496994771677	3.11268639315	0.131438138904	0.201869469039	0.363269131637	-0.256241812279	0.0268307741873	0.333140847609	-0.374050842488	-0.252785611426	-0.138439977003	0.321866454041	0.00885680008539	0.0216229094762	0.15875456079	0.0379154996732	0.268084880393	-2.45669114534	37.4183676784	-10.8875628264
27	4.20288150112	0.130950066912	0.680445790965	5.54834338711	0.609588837686	1.93295183716	-0.0544155860976	-0.0242054911018	-0.36327036077	0.301001170877	-0.048197954523	0.785419990217	-0.151166913481	-0.331350469564	0.0427603877148	0.298793058558	0.0110070688713	0.0792830884564	0.228850002157	-0.0915749459708	0.340441466761	-4.52221686016	18.1827087095	-13.6247706648
28	4.53477639402	5.85044480318	1.58629305217	7.75722915157	2.3398987921	7.56407695545	0.12371828965	-0.00784371589537	0.757407697625	0.315828832073	-0.0146379482753	-0.796010000534	-0.399086425615	0.341832032216	-0.113144260769	0.170398798111	0.00288544784188	-0.0356444331034	0.216511747676	-0.0785976090753	0.465946513328	17.9270435652	0.407595778431	-21.7025192921
29	3.93448210245	-0.971422726691	-1.1108684089	0.463611384633	-0.428243780713	3.22494758587	0.110018323025	0.0231996490406	0.366121147819	0.206002415113	0.180450846446	-0.850117022082	-0.138566930111	0.593365864184	-0.118798698036	0.211791451315	-0.0153942429285	-0.0181544971739	0.26942153269	-0.0884851281653	0.551740571858	19.6940715126	-1.47726930332	-20.5742140381
30	6.75434906286	0.923326606621	-0.846890866325	6.52058863485	-1.38049534038	8.63270218106	-0.344840046506	-0.8008852661	0.425510573304	0.924136997268	0.396932652963	-1.16341823422	0.0157391769163	1.02935689252	0.00747458758425	0.255834597316	0.0907759718348	-0.0792770414844	0.387394382446	-0.120227894062	0.399812436425	25.9085716379	-7.9473843408	-19.6249706579
31	5.03848864899	-1.6422586211	1.35726082198	8.49189470771	-0.669810223241	4.11034499879	-0.0450789810365	0.00649049146906	0.166347004162	0.348997289546	0.0794147014068	-0.702962867442	0.173617493034	0.470561080392	-0.0368081858947	0.191596308268	0.0378873237711	-0.0242375728451	0.335558068892	-0.0394848887038	0.287690438033	24.1583326383	-12.6194385779	-25.5500364415
32	5.70435033804	1.77986841175	3.38861662663	7.27020246959	0.681813497815	5.48756994334	0.21165158633	0.993940933299	1.28866410495	-1.02085757874	-0.266914722929	-1.1398843695	-0.366206115798	0.701294355081	0.0758240387095	0.35608573237	0.0327761268096	0.147361266909	0.49796320357	0.125460567848	0.646460677954	25.7085651778	-2.15231818246	-32.3661858443
33	4.48771159428	-5.14833728065	-0.576536136671	6.0155352749	0.0649692024084	3.15849589836	0.807255380976	0.0780930193369	1.34234537534	-0.267934561018	-0.319331361754	-1.13006937524	-0.606868848744	0.214018408877	-0.452405190666	0.40214191118	-0.153980309591	0.0513334795954	0.441769716546	0.0172831715899	0.961936111824	25.7829332566	6.39143822205	-26.8866620707
34	8.64929944088	-4.00102356751	-6.22779659215	5.16654750985	6.78086595335	9.22912021861	0.892664629668	-0.077685560975	0.893125110328	-0.143347987827	-0.0269993077214	-1.51770328858	-1.36359629957	0.121001698811	-0.898326872101	0.345998731269	-0.0473128812959	0.049227294604	0.232671229099	-0.025997188253	0.508666146739	15.2304184881	9.23610008461	-21.7212399476
35	4.26465799757	-3.77887790022	-0.326983176999	5.50203044963	-1.038550001	0.830676573208	0.228996990047	0.515784225161	-0.268869621333	-0.0649616038984	-0.539946925212	0.409382788157	-0.0244632483685	0.0823735877474	0.320874707832	0.179899769699	0.0171995004016	-0.0180395684783	0.322357800222	-0.0469169382404	0.299501670619	20.1106167799	-12.5102872045	-28.647803924
36	3.17361240939	-0.800719133933	-4.37122241567	4.35585679688	3.66172078854	7.51258325403	0.0857084580955	0.61214555276	-0.854173853374	-0.0124392991848	-0.500972785949	0.736413766749	-0.0446418043998	-0.296684609916	0.463744919015	0.20508638742	-0.0210811171858	-0.0308268354395	0.29700713132	-0.0682435944342	0.287337649232	11.3184158927	-9.19289901677	-30.063233061
37	6.63520937021	-4.26042216046	3.46795382961	2.75953467577	-2.24291057921	1.82530404107	0.363895674956	1.19259411423	0.0610423125416	-1.47193085875	-0.672994320852	1.94642878293	-0.175075544279	-0.461867720287	0.0697324032132	0.363316477456	0.0972477438474	-0.190754587854	0.531551787209	-0.173714904079	0.830788267971	-7.57372855976	3.82909825363	-27.0610835058
38	4.48481688206	-1.06790335282	6.49341668606	4.72946538985	-1.21845491278	9.41760335772	-0.137707425199	0.0686551822409	-0.0226981310543	-0.522948510345	-0.748027573005	0.537288409511	-0.438379479129	-0.17240443865	0.818213022106	0.497697114557	0.162590673028	-0.220149413678	0.549015168793	-0.185857562423	0.737697808298	-12.5319654971	12.2638249715	-24.9201259881
39	5.11653337005	-1.27551803501	-1.34244845489	5.81138866602	1.98605941486	6.80136384979	-0.356763913974	-0.360028614776	-0.237651774217	1.1032275152	0.389590022514	0.0104313928497	0.583189124559	0.612511901953	-0.0366679765875	0.446158704034	0.0432596535249	0.00260901407855	0.17787694444	-0.00334313089493	0.264771751104	9.42977655645	-4.26254956362	-10.9337379846
40	8.60335329665	0.509138786553	4.47131136069	3.96782705895	-4.65062678338	8.46335435552	-0.15436154127	0.547382322715	-0.088997750182	0.684505044447	-0.419684747606	0.0531890240888	0.985147740096	0.30608402833	0.497318352614	0.609519419581	-0.0270299821846	0.186866387646	0.223933410748	-0.0700782156584	0.421328155436	1.42881284077	-7.95840768802	-11.1526569724
41	7.0547201907	1.06618619092	3.08462604828	6.15996260245	2.30886749079	7.18850054053	-0.313435774373	0.111449063332	0.494729688238	0.3121519422	0.135262847103	0.0570212896392	-0.122582849622	0.180462958992	0.204663288034	0.264598363802	-0.00318796181758	0.00794172751807	0.154668785755	-0.0258245917122	0.335304915358	8.17204784767	4.97901382351	-14.9391608337
42	2.51896948574	2.10109334785	-0.453201548656	6.23842775015	0.132853610375	8.11701031413	0.364449717506	0.0187157001205	0.000222279582281	0.615095711549	-0.0693325050448	-1.07693223963	-0.453078339488	0.67612955389	-0.305499618795	0.346088867851	-0.0266919435233	-0.0472499875741	0.277107503581	-0.10088152602	0.498511875869	11.7646894085	15.5619973852	-14.3792579183
43	5.09918640422	-1.44315642948	1.54752783	8.39348109021	-3.86183727882	5.59142030386	0.0849637621389	0.164138263096	-0.612656094905	0.584381935089	0.0460687158333	1.19548411023	-0.0061208640081	-0.0420784191316	-0.136149818262	0.310671754879	-0.0517318204739	0.0990902822259	0.192101880051	-0.0925285750824	0.492712299385	-2.83503028651	5.11839608634	-13.8232274139
44	8.13340618286	-6.04394561338	5.75123260356	9.05142294446	-5.85483635986	4.72412079622	0.0131869392119	-0.156207408778	-0.424455386686	0.0405622128379	0.0275639286845	0.669689656812	0.0987094518995	-0.467007316593	0.0114490070545	0.223113222567	0.016252701994	0.0359286520996	0.249503125441	-0.044252178078	0.272884442296	13.1913024923	40.3072478476	-76.2730258724
45	3.706315172	0.426912816213	-0.300441063007	3.71744363729	-0.0481331059292	3.33127823505	0.0547753703382	0.191835182913	-0.0972097925212	-0.114823120599	-0.0402002118519	-0.317922783918	0.0402119818397	0.216673100314	-0.0242940927965	0.166420663176	0.0352327029074	0.0358546287426	0.179836905138	-0.00927837732833	0.153998495953	29.3819287934	31.6842798604	-80.7317610886
46	7.53291224635	5.19648869785	-0.940250445928	4.42112471059	-2.60130972109	4.84096204109	-0.365009100406	1.61705172841	-0.456754917758	-0.700213259494	-0.341115870399	0.0423357186824	0.0906601364451	-0.856466379887	0.666447956448	0.286289910302	0.0393114187893	-0.0200113512099	0.451347432969	-0.057052910342	0.253941591021	16.177456212	30.2587123616	-88.8976772087
47	7.04141568961	-1.62449682648	0.394724176739	7.76783153493	-0.243990426842	3.77635986477	-0.180270230384	0.224458617577	-0.8083548147	-0.213045830986	0.251062974018	0.753021986925	0.271005100295	-0.300837462668	-0.0898927601453	0.208404459241	-0.065247705077	0.0193876792382	0.289317625426	-0.0195839882827	0.305752879024	15.0871415399	25.1008730967	-79.5741405242
48	3.34262214312	-1.29459819612	-1.75657046263	0.64568552473	-0.318462228041	4.86155287501	0.031751578902	-0.294079840406	-0.149690082516	0.0704148822624	-0.0167681769731	0.0705040376887	0.0135385670117	0.0322486267068	0.0103438038402	0.242209401836	0.0324894311029	-0.000995086804401	0.229843035445	0.0401838935215	0.223948444231	22.4597499145	27.6447038362	-65.5008970318
49	4.18181748358	2.0621916027	1.0556721328	6.25992696926	-0.0397585804649	4.2252671729	-0.108355470359	-0.517237877969	0.0705983430399	-0.0324118752149	-0.133643670711	0.858944392517	-0.171655115483	-0.695813166803	0.240012033182	0.227394202898	0.0392917904533	0.0215599964122	0.425570398144	0.0029697980387	0.290390631545	9.45038866834	30.9112791751	-61.0629207751
50	2.82694000646	0.408265333157	1.56630807453	4.20809634967	-0.42022027156	5.13145076477	0.283084636271	0.0677394034817	-0.0918857695016	0.679898187331	0.217724033925	-0.839163579954	-0.186290273133	-0.0906955271277	-0.350233087172	0.410146027583	0.0452489076155	-0.169135684424	0.374062635979	-0.116325397576	0.435286085261	39.0769125165	26.7564819873	-43.0818291896
51	5.38453794079	0.176813712197	0.0112371891152	6.02588731748	2.14464526204	3.88211412165	-0.0479129540279	-0.385388001893	0.0559753796175	0.322913660113	0.146535976001	-0.931618864388	-0.881052527395	0.735460314159	-0.09582123555	0.711310952088	-0.0350645663824	-0.39233590749	0.564608547781	-0.0864424247858	0.741549299128	45.0165191326	30.1776900556	-36.5538924673
52	5.09842983961	1.11222975585	-4.83234684578	8.05240454137	-0.831520525123	4.58649812254	-0.228067825915	-1.1685620289	-0.340668079754	1.83864412519	-0.900600060523	-0.241732909457	0.149332275905	0.399358919123	0.126046558129	1.19665791432	0.0527045652127	-0.394345663501	0.902436965836	-0.250007905586	0.545953108937	44.314187721	29.7197339792	-28.149325003
53	9.13372199453	0.347406565872	-3.33147365592	8.01461111857	-0.388244785053	5.56333232273	-0.173118188549	-0.857648212744	-0.0908468161641	1.50285294694	-0.143994914159	-0.407420777546	0.549182219776	0.209304133111	0.418245472643	0.769413324035	0.122583633313	-0.416541033643	0.590650690479	-0.0546867710369	0.893914030912	41.8156170071	17.0126994043	-35.8308822641
54	3.638955198	4.35539387255	-3.52575786056	5.27417988344	-4.2901108248	3.48483446476	0.263501893042	-0.579216008051	0.144356671343	0.5105592705	-1.13853554784	-1.18389642468	1.18854716086	2.31243071386	0.966523233642	0.624766750047	0.121762710631	-0.241593674959	0.75589057804	0.113849107995	0.888850628239	49.6021387603	19.5561860597	-41.7409760717
55	6.58429377094	-0.198120264846	-3.56872442581	2.03201334436	0.0793632548653	2.04435932885	-0.250496912897	0.915788271788	-0.846983108721	-0.612358964261	0.0990871411582	-0.268690900364	1.02857898941	-0.246503810294	0.15005873356	0.465977631495	0.0159568635645	-0.0504119437286	0.469801479011	-0.157933070031	0.8078319383	41.0354988641	20.5471291822	-52.455634007
56	4.20135427916	-0.586900876706	1.36560251607	3.7534811174	0.000107226478019	8.0959609309	0.401212649764	0.200696437121	-0.571938205718	0.415037474001	0.30607349411	-0.425279017405	0.441245078808	0.614420112813	-0.633610182978	0.364412586265	0.073150214946	-0.129179783735	0.364124303148	-0.104232422712	0.484586195115	36.1505887681	23.2747751622	-44.5214009917
57	7.59346623489	1.96935538142	0.46406852802	2.65488584147	0.649520721883	2.01613276521	-0.735054011841	-1.10007046044	-0.221063218122	0.519946145192	0.29126463074	-1.33108246017	-0.834914198486	0.0981022462998	0.339784342365	0.771660677843	0.292478849008	-0.256022404236	1.18818110837	-0.366318688633	0.889542575139	40.0968267886	21.9559740342	-23.1356634673
58	7.06317630646	-3.45329143104	5.10820353094	1.67782618982	-2.56137473082	6.91459282777	-0.634199805975	-1.36541370135	-1.01765772616	1.12959867894	0.621116950438	-0.117288050911	-0.00506928589971	-1.45199513934	-0.216954994404	0.915261297746	-0.118334092543	-0.257595462335	0.777723256624	0.289459096718	0.578151364722	26.2619158189	20.1521537718	-36.4046496328
59	9.43434624323	-3.6751237603	6.15064322763	3.97203201648	-1.24793444486	4.52274689487	-0.518194756896	-1.06709888145	-0.920400671159	-0.0351955868102	0.636333908517	1.54117633211	1.14283193856	-1.92402965011	-0.19809185305	0.663373216528	-0.346186572894	-0.224277741336	0.983525783409	0.344336191782	0.659377345263	8.83744466555	19.7620828297	-52.882459224
60	4.72972009611	-1.40062420931	3.11278765825	3.39349354436	1.53766120271	4.01215490621	-0.312434979426	-0.878065658534	0.429343764716	0.690842305469	0.155525376801	-0.250918653517	-0.679265315816	-0.392964931094	0.0769441768284	0.561902265534	0.118736878747	-0.11949292525	0.479370916091	-0.101807107858	0.318431478734	30.3960783387	35.9736652281	-41.5650213237
61	2.8364956017	-0.43034380438	0.373569984708	2.42417301463	0.181541809913	4.84233660651	-0.124363234132	-0.871375518382	0.614731365409	0.991437091683	0.24196169883	0.0146177736954	-1.16613263442	-1.02735965359	0.0806625113782	0.601074142647	0.222513039379	-0.0598596613811	0.621527630211	-0.151054232262	0.41173022383	23.3584526997	40.4204026963	-42.0646009161
62	4.74772451111	0.448427743081	0.990139522426	5.26465262287	0.182532640821	5.69866662	-0.275620774875	-0.0266747096494	-0.0532399427266	0.492687590403	0.380637536052	-0.316492674562	-0.0452639264595	-0.257174354755	-0.10090089596	0.299271998264	0.0525645012256	-0.0267511257352	0.395969942367	-0.0542487863656	0.25557599043	27.8969548846	31.0266661085	-50.0788042311
63	2.84553310149	-1.16893304462	-2.84629292309	7.19255462555	1.32202257622	3.06110259852	0.205049985043	0.451668253868	0.143281916641	-0.434253276554	0.0475398378342	-0.970407438908	-0.296359695805	0.742403009649	-0.155314885832	0.376403407878	-0.0210418983541	-0.0270682908949	0.606160318487	0.0565754309676	0.419112724912	32.1160894251	29.1035981443	-60.8760183569
64	5.16332395359	0.999714082172	0.993076833598	6.03551113265	3.02543762804	3.6295952313	-0.117705926518	-0.839369362167	0.0205643467474	0.0884836495091	-0.0780910911144	0.0207332036421	-0.29700995394	-0.821086222892	0.198521370247	0.354621112916	0.147225974845	-0.0123096957593	0.579799230216	0.0102607815515	0.201004369792	17.4259475898	31.9707000968	-51.0809473839
65	9.69537734566	5.89864438402	6.11184883654	4.23925851743	5.19258755124	8.53788853157	0.0232829758502	-0.12839124496	0.170633866987	0.119932853098	0.0332364106613	0.48990158699	0.281183246609	-0.632592258428	-0.0894006189922	0.316942866208	-0.00854057474952	-0.0370911441645	0.291857130837	0.0135313519364	0.328412466729	-30.8498063217	-28.0657108381	-84.2074362193
66	2.22295764413	-0.146241164229	-0.43809800917	4.2460248909	0.75271239418	4.1925611117	0.0552525787436	0.0677259594248	-0.144374641438	-0.121290459001	0.0194369725126	-0.217185663645	0.0936201791542	0.423350719977	-0.0841620366412	0.180042330755	0.0304433179813	0.000619336631712	0.194943870879	-0.0292334016723	0.177908214873	-14.3777505718	-31.2551971791	-74.8622495282
67	6.58495211307	-1.24949344435	1.02339627495	3.21149229849	0.606236127189	4.81732969143	-0.0348327647557	-0.295393890354	-0.522612412052	0.358480503967	-0.0441944550126	0.56084494263	0.490500669224	-0.515574535024	0.0666502538262	0.266026600415	-0.0542060955341	0.0592525977724	0.223556893937	0.0112778149846	0.245885939042	-27.727188115	-35.4943828161	-72.0270722782
68	3.4622495721	0.2271049833	-0.418518788601	4.99225733893	-5.01363674081	4.98354551167	-0.0447613193902	-0.0892590588911	-0.279887518842	0.0629581511142	-0.0482653748919	-0.427022341281	0.0244100812786	-0.30601773845	0.04807387237	0.290274426718	0.00329188510368	-0.0117690627105	0.252256668295	-0.0735810503117	0.192679462373	-19.4350896604	-23.5696724163	-69.0845819956
69	3.87091347859	1.08867871872	-3.06478483206	2.6428549845	-0.717282631017	3.71273654011	0.0859554080248	-0.0355606710932	0.617873257455	0.0868764014349	0.126711117175	0.620508238305	0.0625517172444	-0.258620926212	-0.16217550682	0.15551038054	0.00123562697986	0.000964539435475	0.202625647305	-0.0303330744399	0.312776735337	-33.3559264613	-11.5280119658	-70.1175186952
70	9.07616605735	-2.90539861899	-0.812824015817	8.41424498771	-0.968093689476	4.75488181758	-0.157683314474	0.790900457747	0.214927753958	-0.463019734329	0.340645539286	1.06006550095	0.086482183852	-0.594799446525	-0.163554053028	0.214846444689	-0.0346013429723	-0.0664060683388	0.336531824148	0.0197633304402	0.378714605962	-34.7272795198	-14.3420537218	-75.4497485998
71	5.10124572364	-0.729071999487	0.200767221584	5.11000304826	1.3486338436	5.41752227187	-0.226147106287	0.096497679452	-0.236876512267	0.0131955722614	-0.0539605988234	0.315686234947	-0.0323410331586	0.264094490192	0.208032359314	0.198377125915	-0.0262847397156	-0.00929020539766	0.241637988548	0.0193105119391	0.304303331996	-8.30577830036	3.46978795573	-66.3235864282
72	6.0471601717	-0.457806003522	1.52751488813	2.52069093027	-0.231228283877	2.74442692155	-0.0434227402019	0.237943677032	0.464197132744	-0.0151506887799	-0.0427736331033	-0.210049627329	0.0729077937314	0.18818511386	0.0460362256547	0.216960199175	-0.00182024432046	0.0312038404736	0.161658171408	0.0276635352572	0.270570680792	-6.61228740369	5.31645061935	-67.6544689393
73	5.44981345753	0.42087485757	0.692641937332	7.58216946369	-0.604913487527	6.05317221825	-0.30321226116	0.494139025135	0.366556922494	-0.861511932124	0.21979276232	-1.07944784665	0.136243410334	0.73467020732	-0.0237051302593	0.316646048588	-0.0716308337431	0.0734763190273	0.331423226175	-0.0111256716109	0.438514158635	-0.697146405575	11.9377968515	-69.3586559004
74	4.79441966916	0.745130051196	-0.340148380595	7.82570743053	-0.719076466746	5.82754702439	-0.0621667885949	0.0428406509758	0.758979939158	-0.0389745296929	0.229775713747	-0.321581030708	-0.458081160998	0.446456793679	-0.0485505425354	0.288756302903	-0.0180616583386	0.00696306768874	0.257041199798	-0.0290421576537	0.487283703837	-2.46739910448	17.0541366672	-63.7615420041
75	8.5041721846	-0.447667664935	-2.95819992879	7.33899241479	2.46527550348	2.13969827584	0.0278171024982	-1.4903469274	0.44487405276	1.15754519592	0.330540493324	-0.729772520686	0.153685952445	0.519150786707	-0.380324654914	0.411990159736	0.0423069008481	-0.116380211789	0.505542084842	-0.0969741424209	0.471861349627	-0.841912500748	7.54354435716	-55.9115757095
76	5.65242954915	1.73816140303	0.107663621837	5.22177017929	3.71703257051	5.39630941137	0.177203832642	-0.243593776396	-0.355780535701	1.07412010705	0.417964824475	-0.712074994432	0.419170029866	0.00178252323019	-0.582808719337	0.435020067759	0.114971850638	-0.0198570346847	0.350251272728	0.0467264899998	0.373298399137	-0.132622316134	-2.10960600313	-60.9410488333
77	1.42400187714	2.03728843879	0.150592581687	7.80166611347	2.29148355662	1.59313977104	-0.159121056023	-0.0427032073464	0.21315440221	-0.00949487349545	0.189631097893	0.0616771076713	0.0715393981518	0.145543061551	-0.0456999400772	0.209013545651	0.0273980932549	0.00118819269528	0.286295444222	0.00758651873137	0.286917306959	-9.26436492388	1.29773209407	-63.9568105133
78	8.28245202404	4.57183484381	-0.812304273821	4.33213192248	1.77480374714	3.05731095246	0.423826907808	-0.806128461083	2.50099495819	0.973534789658	-0.566968140128	1.73045833716	-1.43216385537	-0.0635902016855	-0.000462458311275	0.499556552954	-0.00885828233127	0.00336565478978	0.486799231243	-0.107648263951	0.922743073608	-4.01008474277	26.6298432142	-58.6640458118
79	4.63633834599	6.09813740119	1.64164097549	7.95478172258	2.09763857251	2.67658057796	0.00669425317682	-0.243539202841	1.56574496838	0.225045624122	-0.325867150376	0.821688452387	-0.140071035338	-0.122227330153	0.226921301562	0.28033154755	0.0103926411511	-0.000566835562971	0.31517150097	-0.0877840193315	0.466431373507	-15.1091382492	13.5063683785	-58.611113874
80	1.37782570366	-0.805743163734	-0.335584288629	0.773979622801	1.25781721835	3.60794393237	0.188779840855	-0.839244471201	0.182807316609	0.960759228418	-0.687059704909	1.63510800844	0.823589408406	-0.758964313541	0.409795303379	0.422262661952	-0.152388809928	0.289632661617	0.66038263469	-0.263410414834	0.712818713715	-35.118549143	-5.51892203305	-59.7998610651
81	6.67605155721	-0.539129439093	3.04575271404	6.32452585421	-0.37876013865	1.58633408451	-0.269663580468	0.437707574084	1.15282077342	-0.368106387378	0.14746967149	0.326763118588	-0.345720117323	-0.0731402487391	0.0474639104535	0.280129198989	-0.00610158166468	-0.0842190097355	0.176315928785	0.0418552157757	0.316457332013	-18.0232872611	10.2143060251	-74.027440926
82	3.9399959371	2.33209574105	2.50740343565	5.94630054236	1.70819041939	6.81169059739	-0.443083984867	0.676944948484	0.394115755118	-0.949157950052	0.150753964892	0.323562914181	-0.628483213223	0.456627551718	0.25016970874	0.34234315768	-0.00641419458868	-0.0447935972973	0.286025212131	0.0851829263315	0.260161141383	-16.8699190209	4.258433334	-80.1246260854
83	7.68642602404	1.14879392092	-1.99915974711	6.8533887088	-3.67448886858	2.37879378561	-0.335250878461	-0.379009191254	0.803408792537	-0.184946970074	-0.323147190948	-0.198263403813	-0.768026971128	0.529775495591	0.639366093576	0.346578054434	-0.00675471213749	-0.110102872437	0.287171794968	0.0382284114829	0.455930066656	-25.1634670474	9.98303982105	-76.7928745674
84	7.0915314115	-0.886184245673	-2.26440266232	6.07255425494	1.3102214779	7.07030141005	-0.201941515449	0.0214898351182	-0.055376020159	-0.0504396674156	0.00741894537896	-0.0144740274138	0.130165881157	0.175811400279	0.178575597859	0.163575505135	0.00168222221596	0.00145111907206	0.149554118656	0.0165907243862	0.18321310098	-17.3450581991	-3.00814124057	-72.3559438774
85	1.53920829531	-2.43742706189	0.355114848979	7.58466304151	0.929044555191	7.5609679958	0.627853086646	0.485199467862	-0.0936178164568	-0.515594187937	-0.255651163422	-0.837983863813	0.226680566996	0.128869835388	-0.293680393212	0.24305559949	0.00373785341797	0.0205154473267	0.256938250796	-0.0669965722278	0.325743066536	-14.2560139229	-12.8018701921	-71.4145424113
86	6.75435213755	1.24073957926	-0.988866001297	8.61503114365	-3.79214408907	8.35924815319	0.10185517136	-0.0778009100363	0.753137387268	-0.192398860451	0.0108533090639	0.86423438471	-0.0303380653136	-0.0577348771333	-0.0875288841692	0.150669411138	0.0203039415755	-0.0367412698816	0.188697231314	-0.0325639349432	0.354285984145	-28.9345631653	-3.04078368005	-72.7876881927

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 30 through 46 )	A	A	30 - 46	1 - 17
2	2	chain 'A' and (resid 47 through 90 )	A	A	47 - 90	18 - 61
3	3	chain 'A' and (resid 91 through 100 )	A	A	91 - 100	62 - 71
4	4	chain 'A' and (resid 101 through 112 )	A	A	101 - 112	72 - 83
5	5	chain 'A' and (resid 113 through 134 )	A	A	113 - 134	84 - 105
6	6	chain 'A' and (resid 135 through 162 )	A	A	135 - 162	106 - 120
7	7	chain 'A' and (resid 163 through 198 )	A	A	163 - 198	121 - 156
8	8	chain 'B' and (resid 1 through 23 )	B	B	1 - 23	1 - 23
9	9	chain 'B' and (resid 24 through 35 )	B	B	24 - 35	24 - 35
10	10	chain 'B' and (resid 36 through 45 )	B	B	36 - 45	36 - 45
11	11	chain 'B' and (resid 46 through 55 )	B	B	46 - 55	46 - 55
12	12	chain 'B' and (resid 56 through 68 )	B	B	56 - 68	56 - 68
13	13	chain 'B' and (resid 69 through 79 )	B	B	69 - 79	69 - 79
14	14	chain 'B' and (resid 80 through 84 )	B	B	80 - 84	80 - 84
15	15	chain 'B' and (resid 85 through 94 )	B	B	85 - 94	85 - 94
16	16	chain 'B' and (resid 95 through 99 )	B	B	95 - 99	95 - 99
17	17	chain 'B' and (resid 100 through 104 )	B	B	100 - 104	100 - 104
18	18	chain 'C' and (resid 16 through 22 )	C	C	16 - 22	1 - 7
19	19	chain 'C' and (resid 23 through 44 )	C	C	23 - 44	8 - 29
20	20	chain 'C' and (resid 45 through 66 )	C	C	45 - 66	30 - 39
21	21	chain 'C' and (resid 67 through 83 )	C	C	67 - 83	40 - 56
22	22	chain 'C' and (resid 84 through 96 )	C	C	84 - 96	57 - 69
23	23	chain 'C' and (resid 97 through 112 )	C	C	97 - 112	70 - 85
24	24	chain 'D' and (resid 30 through 46 )	D	D	30 - 46	1 - 17
25	25	chain 'D' and (resid 47 through 90 )	D	D	47 - 90	18 - 61
26	26	chain 'D' and (resid 91 through 134 )	D	D	91 - 134	62 - 104
27	27	chain 'D' and (resid 135 through 198 )	D	D	135 - 198	105 - 156
28	28	chain 'E' and (resid 1 through 9 )	E	E	1 - 9	1 - 9
29	29	chain 'E' and (resid 10 through 23 )	E	E	10 - 23	10 - 23
30	30	chain 'E' and (resid 24 through 35 )	E	E	24 - 35	24 - 35
31	31	chain 'E' and (resid 36 through 45 )	E	E	36 - 45	36 - 45
32	32	chain 'E' and (resid 46 through 55 )	E	E	46 - 55	46 - 55
33	33	chain 'E' and (resid 56 through 65 )	E	E	56 - 65	56 - 65
34	34	chain 'E' and (resid 66 through 72 )	E	E	66 - 72	66 - 72
35	35	chain 'E' and (resid 73 through 84 )	E	E	73 - 84	73 - 84
36	36	chain 'E' and (resid 85 through 94 )	E	E	85 - 94	85 - 94
37	37	chain 'E' and (resid 95 through 99 )	E	E	95 - 99	95 - 99
38	38	chain 'E' and (resid 100 through 104 )	E	E	100 - 104	100 - 104
39	39	chain 'F' and (resid 17 through 44 )	F	F	17 - 44	1 - 28
40	40	chain 'F' and (resid 45 through 66 )	F	F	45 - 66	29 - 38
41	41	chain 'F' and (resid 67 through 82 )	F	F	67 - 82	39 - 54
42	42	chain 'F' and (resid 83 through 96 )	F	F	83 - 96	55 - 68
43	43	chain 'F' and (resid 97 through 112 )	F	F	97 - 112	69 - 84
44	44	chain 'G' and (resid 30 through 45 )	G	G	30 - 45	1 - 16
45	45	chain 'G' and (resid 46 through 100 )	G	G	46 - 100	17 - 71
46	46	chain 'G' and (resid 101 through 112 )	G	G	101 - 112	72 - 83
47	47	chain 'G' and (resid 113 through 134 )	G	G	113 - 134	84 - 105
48	48	chain 'G' and (resid 135 through 174 )	G	G	135 - 174	106 - 131
49	49	chain 'G' and (resid 175 through 198 )	G	G	175 - 198	132 - 155
50	50	chain 'H' and (resid 1 through 23 )	H	H	1 - 23	1 - 23
51	51	chain 'H' and (resid 24 through 35 )	H	H	24 - 35	24 - 35
52	52	chain 'H' and (resid 36 through 41 )	H	H	36 - 41	36 - 41
53	53	chain 'H' and (resid 42 through 50 )	H	H	42 - 50	42 - 50
54	54	chain 'H' and (resid 51 through 60 )	H	H	51 - 60	51 - 60
55	55	chain 'H' and (resid 61 through 68 )	H	H	61 - 68	61 - 68
56	56	chain 'H' and (resid 69 through 78 )	H	H	69 - 78	69 - 78
57	57	chain 'H' and (resid 79 through 86 )	H	H	79 - 86	79 - 83
58	58	chain 'H' and (resid 87 through 96 )	H	H	87 - 96	84 - 93
59	59	chain 'H' and (resid 97 through 104 )	H	H	97 - 104	94 - 101
60	60	chain 'I' and (resid 17 through 38 )	I	I	17 - 38	1 - 22
61	61	chain 'I' and (resid 39 through 66 )	I	I	39 - 66	23 - 39
62	62	chain 'I' and (resid 67 through 82 )	I	I	67 - 82	40 - 55
63	63	chain 'I' and (resid 83 through 96 )	I	I	83 - 96	56 - 69
64	64	chain 'I' and (resid 97 through 112 )	I	I	97 - 112	70 - 85
65	65	chain 'J' and (resid 30 through 46 )	J	J	30 - 46	1 - 17
66	66	chain 'J' and (resid 47 through 90 )	J	J	47 - 90	18 - 61
67	67	chain 'J' and (resid 91 through 133 )	J	J	91 - 133	62 - 104
68	68	chain 'J' and (resid 134 through 162 )	J	J	134 - 162	105 - 119
69	69	chain 'J' and (resid 163 through 184 )	J	J	163 - 184	120 - 141
70	70	chain 'J' and (resid 185 through 198 )	J	J	185 - 198	142 - 155
71	71	chain 'K' and (resid 1 through 9 )	K	K	1 - 9	1 - 9
72	72	chain 'K' and (resid 10 through 23 )	K	K	10 - 23	10 - 23
73	73	chain 'K' and (resid 24 through 35 )	K	K	24 - 35	24 - 35
74	74	chain 'K' and (resid 36 through 45 )	K	K	36 - 45	36 - 45
75	75	chain 'K' and (resid 46 through 55 )	K	K	46 - 55	46 - 55
76	76	chain 'K' and (resid 56 through 65 )	K	K	56 - 65	56 - 65
77	77	chain 'K' and (resid 66 through 79 )	K	K	66 - 79	66 - 79
78	78	chain 'K' and (resid 80 through 84 )	K	K	80 - 84	80 - 84
79	79	chain 'K' and (resid 85 through 94 )	K	K	85 - 94	85 - 94
80	80	chain 'K' and (resid 95 through 104 )	K	K	95 - 104	95 - 104
81	81	chain 'L' and (resid 16 through 27 )	L	L	16 - 27	1 - 12
82	82	chain 'L' and (resid 28 through 45 )	L	L	28 - 45	13 - 30
83	83	chain 'L' and (resid 46 through 66 )	L	L	46 - 66	31 - 40
84	84	chain 'L' and (resid 67 through 83 )	L	L	67 - 83	41 - 57
85	85	chain 'L' and (resid 84 through 96 )	L	L	84 - 96	58 - 70
86	86	chain 'L' and (resid 97 through 112 )	L	L	97 - 112	71 - 86

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