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- PDB-7xvn: Structural basis for DNA recognition feature of retinoid-related ... -

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Basic information

Entry
Database: PDB / ID: 7xvn
TitleStructural basis for DNA recognition feature of retinoid-related orphan receptors
Components
  • DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')
  • DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')
  • Nuclear receptor ROR-gamma
KeywordsDNA BINDING PROTEIN/DNA / nuclear receptor / transcription factor / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


regulation of gamma-delta T cell differentiation / mucosa-associated lymphoid tissue development / Nuclear Receptor transcription pathway / T-helper 17 cell differentiation / regulation of steroid metabolic process / Peyer's patch development / positive regulation of circadian rhythm / T-helper cell differentiation / oxysterol binding / negative regulation of thymocyte apoptotic process ...regulation of gamma-delta T cell differentiation / mucosa-associated lymphoid tissue development / Nuclear Receptor transcription pathway / T-helper 17 cell differentiation / regulation of steroid metabolic process / Peyer's patch development / positive regulation of circadian rhythm / T-helper cell differentiation / oxysterol binding / negative regulation of thymocyte apoptotic process / regulation of fat cell differentiation / alpha-beta T cell differentiation / regulation of glucose metabolic process / adipose tissue development / lymph node development / xenobiotic metabolic process / circadian regulation of gene expression / transcription coactivator binding / nuclear receptor activity / T cell differentiation in thymus / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / external side of plasma membrane / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / zinc ion binding / nucleoplasm / nucleus
Similarity search - Function
Nuclear receptor ROR / Retinoid-related orphan receptors, DNA-binding domain / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily ...Nuclear receptor ROR / Retinoid-related orphan receptors, DNA-binding domain / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type
Similarity search - Domain/homology
DNA / DNA (> 10) / Nuclear receptor ROR-gamma
Similarity search - Component
Biological speciesMus musculus (house mouse)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.302 Å
AuthorsChen, Y. / Jiang, L.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81974074, 82172654 China
CitationJournal: Structure / Year: 2024
Title: Structural characterization of the DNA binding mechanism of retinoic acid-related orphan receptor gamma.
Authors: Jiang, L. / Liu, X. / Liang, X. / Dai, S. / Wei, H. / Guo, M. / Chen, Z. / Xiao, D. / Chen, Y.
History
DepositionMay 24, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 29, 2023Provider: repository / Type: Initial release
Revision 1.1Feb 14, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Apr 17, 2024Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Nuclear receptor ROR-gamma
B: Nuclear receptor ROR-gamma
N: DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')
P: DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')
C: Nuclear receptor ROR-gamma
D: Nuclear receptor ROR-gamma
E: DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')
F: DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,04816
Polymers77,5248
Non-polymers5238
Water2,144119
1
A: Nuclear receptor ROR-gamma
B: Nuclear receptor ROR-gamma
N: DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')
P: DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,0248
Polymers38,7624
Non-polymers2624
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5480 Å2
ΔGint-29 kcal/mol
Surface area13220 Å2
MethodPISA
2
C: Nuclear receptor ROR-gamma
D: Nuclear receptor ROR-gamma
E: DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')
F: DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,0248
Polymers38,7624
Non-polymers2624
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5710 Å2
ΔGint-66 kcal/mol
Surface area12840 Å2
MethodPISA
Unit cell
Length a, b, c (Å)33.627, 64.584, 238.635
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein
Nuclear receptor ROR-gamma / Nuclear receptor RZR-gamma / Nuclear receptor subfamily 1 group F member 3 / RAR-related orphan ...Nuclear receptor RZR-gamma / Nuclear receptor subfamily 1 group F member 3 / RAR-related orphan receptor C / Retinoid-related orphan receptor-gamma / Thymus orphan receptor / TOR


Mass: 13864.976 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rorc, Nr1f3, Rorg, Thor / Production host: Escherichia coli (E. coli) / References: UniProt: P51450
#2: DNA chain DNA (5'-D(P*CP*AP*TP*GP*AP*CP*CP*TP*AP*CP*TP*GP*AP*CP*CP*TP*AP*G)-3')


Mass: 5460.556 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#3: DNA chain DNA (5'-D(P*CP*TP*AP*GP*GP*TP*CP*AP*GP*TP*AP*GP*GP*TP*CP*AP*TP*G)-3')


Mass: 5571.614 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 119 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.67 Å3/Da / Density % sol: 26.4 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5.6
Details: 0.1 M MES pH 5.6, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 22% PEG 8K

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL10U2 / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER2 S 9M / Detector: PIXEL / Date: Dec 30, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.302→32 Å / Num. obs: 43676 / % possible obs: 99 % / Redundancy: 7.1 % / CC1/2: 0.995 / Rmerge(I) obs: 0.1416 / Net I/σ(I): 11.33
Reflection shellResolution: 2.302→2.384 Å / Num. unique obs: 2370 / CC1/2: 0.683

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
PDB_EXTRACT3.27data extraction
autoPROCdata reduction
autoPROCdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6L6L

6l6l


Resolution: 2.302→32 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 27.87 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2559 3668 8.4 %
Rwork0.2078 40008 -
obs0.2117 43676 98.16 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 128.34 Å2 / Biso mean: 54.9235 Å2 / Biso min: 23.06 Å2
Refinement stepCycle: final / Resolution: 2.302→32 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2270 1476 8 119 3873
Biso mean--46.72 42.55 -
Num. residues----376
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0073954
X-RAY DIFFRACTIONf_angle_d0.6725615
X-RAY DIFFRACTIONf_chiral_restr0.043622
X-RAY DIFFRACTIONf_plane_restr0.004476
X-RAY DIFFRACTIONf_dihedral_angle_d22.9022125
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.302-2.33210.3261420.3212153999
2.3321-2.3640.36181390.321154598
2.364-2.39780.35211530.3272160799
2.3978-2.43360.39371370.3145150098
2.4336-2.47160.3331360.31151099
2.4716-2.51210.36421420.3008157799
2.5121-2.55540.31791390.3096153399
2.5554-2.60180.31751480.2876160499
2.6018-2.65190.3391340.2899148498
2.6519-2.7060.32321430.2912154598
2.706-2.76480.27381400.2706153898
2.7648-2.8290.31751420.2754157898
2.829-2.89970.32981420.2707150698
2.8997-2.97810.34181390.2638151898
2.9781-3.06570.31471470.2699154797
3.0657-3.16450.27991420.2469152999
3.1645-3.27750.30811370.227152298
3.2775-3.40860.2841370.2028151995
3.4086-3.56350.26551440.1951151098
3.5635-3.75110.24011440.1858159199
3.7511-3.98580.22611400.1706152199
3.9858-4.29280.22461440.1553157899
4.2928-4.72360.19061410.1434152699
4.7236-5.40430.17991430.1436154999
5.4043-6.79810.18221420.1624155298
6.7981-320.20431310.1594148094
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.35793.5812-1.88986.1713-1.83466.9825-0.2870.0599-0.2843-0.23610.0765-1.2529-0.04770.49960.1830.3253-0.00310.04910.4516-0.10390.520713.1172-15.7101-71.8331
22.13232.3479-1.1624.464-1.27014.2928-0.19060.4391-0.31090.15720.2201-0.1566-0.23770.438-0.07040.20540.0072-0.00080.2575-0.0570.37339.2684-9.981-66.9031
35.97023.2813-0.44988.7674-1.61659.34390.28890.61840.2089-0.2074-0.06220.8204-0.87690.2175-0.29230.3676-0.00290.0250.31150.01870.41676.7392-2.3699-73.3396
47.05261.65610.49544.9158-3.41467.3501-0.45140.87570.7271-0.27920.37020.86480.3894-0.78370.08450.7129-0.1429-0.1350.69140.01910.64471.2705-9.7746-85.5826
54.9112-0.48952.26346.7963-2.87544.98580.00580.9461-0.062-1.72210.5495-0.10980.63270.73240.24110.6376-0.09690.09350.5283-0.05520.624411.1194-10.4595-84.2511
62.30020.2683-1.02855.114-3.29582.8219-0.53570.87050.3746-0.71080.14240.65530.3971-0.5892-0.09560.3833-0.0876-0.06390.4558-0.02410.40071.56-9.7153-78.9766
72.98211.8660.75077.0027-2.1153.9685-0.08050.32810.08040.30550.23590.7209-0.4019-0.5227-0.05040.2813-0.0514-0.00740.4629-0.00980.439-0.5313-7.336-66.699
86.1322-2.38192.62231.9957-3.36849.59860.0513-0.67-0.82360.28110.34090.52842.19180.2991-0.43190.68250.0241-0.00430.6032-0.0380.46916.8091-12.1083-53.861
95.8431-0.81120.99272.77121.05187.8807-0.0778-0.2799-0.45880.183-0.17730.1186-0.09080.16460.3050.30750.02920.00610.39350.01910.3607-2.522-1.3816-42.0896
107.7224-0.1430.84731.9690.18682.19080.2552-0.33260.8669-0.0468-0.2373-0.2442-0.3111.08850.16190.32770.00380.03970.4537-0.03580.4187.85745.1788-45.4874
110.9134-1.78990.6243.981-3.08827.70040.00790.0146-0.1086-0.1746-0.3221-0.8633-0.27110.30250.19340.47380.0055-0.06830.45560.01440.4749-1.211411.0529-56.5825
124.5139-1.2318-0.23243.9143-1.6654.9336-0.0160.16330.18370.0107-0.0931-0.0566-0.2374-0.0220.0240.22540.0661-0.00030.335-0.05870.3239-0.34956.4426-50.2136
137.90883.6495-1.81586.43411.71916.962-0.07910.14920.1636-0.42760.56380.1158-0.3282-0.5347-0.3970.4428-0.14370.01670.4526-0.05240.314813.97470.9971-79.4226
143.5823-0.716-0.38431.96842.15737.07360.0298-0.72330.13480.42370.4525-0.09970.2490.9351-0.34880.30420.0856-0.07890.455-0.08180.368914.2899-2.8919-48.876
154.8367-1.995-0.99351.42731.76862.83120.0396-0.73270.41880.12110.5554-0.19830.24580.8141-0.65460.3996-0.0134-0.01820.5573-0.08870.40512.8377-4.0268-44.1203
164.80691.8103-2.86883.5124-0.14042.48630.4130.52210.2203-0.35280.2962-0.1293-0.87480.5127-0.71390.5013-0.1494-0.01310.6349-0.03650.423116.3064.0001-73.4286
176.3413-0.60530.32314.88541.79452.22950.2633-1.20460.2762-0.0627-0.1301-0.0156-2.09541.4693-0.04831.0463-0.24360.14250.8567-0.08650.450929.26392.0063-129.9226
186.62691.4103-1.31773.2123-0.42665.30140.07560.6375-0.0374-0.7410.1762-0.149-0.7410.1074-0.0130.6072-0.01020.00270.5266-0.02370.361222.67940.1672-125.6136
195.2373-2.9371-2.00342.0398-1.63932.6875-0.06310.09220.277-0.2341-0.07730.8512-1.1417-0.4660.070.73620.0937-0.11880.6009-0.03340.529614.531-2.3424-132.0113
201.15612.06190.60396.25374.58119.05720.0059-0.3982-0.3395-0.9878-0.11040.0103-0.30680.76740.45250.85180.13830.0290.6870.05960.422421.648-9.7118-143.1045
211.0715-0.48850.96750.98920.62742.6590.25870.41360.4872-0.4018-0.24050.4160.040.09150.4551.20910.15710.04110.69880.0050.470922.571-0.9863-141.9756
225.2150.38652.00241.1923-1.63775.36020.40850.5968-0.6813-0.707-0.3995-0.21560.4395-0.1503-0.19870.68620.19850.00630.5923-0.03430.479722.1068-9.5036-136.0191
235.8047-1.9511-0.04462.4197-1.50621.93770.7539-0.0898-0.84280.0758-0.17690.8780.7440.9315-0.79380.64710.13690.02370.44410.01560.637119.831-9.5429-124.5361
242.9996-2.989-3.96834.74642.78776.1864-0.31990.1608-0.62230.55920.705-1.335-0.26381.0969-0.53310.6964-0.0198-0.01340.70060.05640.680821.1932-0.8529-111.8779
252.98421.10311.14436.22440.91666.54460.0022-0.65980.09560.7931-0.3518-0.72480.93410.1487-0.38110.68620.0177-0.05560.4670.05840.653313.7436-11.0141-102.2822
260.9329-1.28891.45645.7619-1.07458.09840.0144-0.1728-0.21380.304-0.01460.4208-0.2555-0.1566-0.24860.5129-0.04880.05560.36620.0640.47077.5477-1.8304-101.8902
270.5380.3822-1.37730.6332-0.79615.26180.35910.40980.0128-0.40740.06911.1136-0.89060.1726-0.31070.620.10410.01441.05280.21191.0008-1.45452.5904-111.994
285.4916-0.1266-1.4245.83820.82297.9839-0.01350.4463-0.5188-0.3754-0.08180.51790.7911-0.592-0.04670.5822-0.086-0.0040.4918-0.01330.53272.9659-8.3212-111.0245
293.8993-2.1085-2.02316.729-1.6192.33210.21191.1553-0.449-1.6449-0.2795-0.044-1.2968-1.03010.33891.190.203-0.05130.8146-0.02850.359312.70354.354-139.0304
306.6405-0.0405-3.48932.5623-1.70655.29060.82950.40461.27980.03160.96280.7794-1.7591-0.5382-0.77891.17520.18940.07530.62130.0290.55219.778810.2717-122.0785
316.2275-0.8627-0.1065.0338-1.95778.82340.1558-0.94130.1012-0.17310.3156-0.1957-0.38590.5889-0.51240.4951-0.060.00520.5439-0.03310.430817.38862.9424-106.1073
322.0094-6.5131-4.41085.56830.98029.4562-0.0583-0.96881.13131.23422.0085-0.0141.17410.2304-1.05071.41520.07780.15520.6840.05620.563616.595610.4154-91.6838
336.36782.747-2.32578.3643-1.4812.21490.7027-0.12170.0049-0.1089-0.07840.7876-0.45361.4324-0.39150.8143-0.09660.07920.67630.03290.429112.02434.4033-95.6114
346.4953-1.6359-3.14939.86675.41293.96820.7174-0.56980.20790.22810.7018-0.731-1.07630.2796-1.37430.91-0.050.03890.52810.03480.451917.92458.7159-112.1088
354.56830.204-0.67055.409-3.7882.77650.13530.55610.0185-0.00310.82910.2462-1.5975-1.3296-0.90471.14010.2137-0.0210.6887-0.06420.4239.22857.3789-133.6084
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 28 through 37 )A28 - 37
2X-RAY DIFFRACTION2chain 'A' and (resid 38 through 48 )A38 - 48
3X-RAY DIFFRACTION3chain 'A' and (resid 49 through 60 )A49 - 60
4X-RAY DIFFRACTION4chain 'A' and (resid 61 through 76 )A61 - 76
5X-RAY DIFFRACTION5chain 'A' and (resid 77 through 83 )A77 - 83
6X-RAY DIFFRACTION6chain 'A' and (resid 84 through 93 )A84 - 93
7X-RAY DIFFRACTION7chain 'A' and (resid 94 through 100 )A94 - 100
8X-RAY DIFFRACTION8chain 'A' and (resid 101 through 111 )A101 - 111
9X-RAY DIFFRACTION9chain 'B' and (resid 28 through 48 )B28 - 48
10X-RAY DIFFRACTION10chain 'B' and (resid 49 through 61 )B49 - 61
11X-RAY DIFFRACTION11chain 'B' and (resid 62 through 76 )B62 - 76
12X-RAY DIFFRACTION12chain 'B' and (resid 77 through 98 )B77 - 98
13X-RAY DIFFRACTION13chain 'N' and (resid 620 through 624 )N620 - 624
14X-RAY DIFFRACTION14chain 'N' and (resid 625 through 637 )N625 - 637
15X-RAY DIFFRACTION15chain 'P' and (resid 611 through 620 )P611 - 620
16X-RAY DIFFRACTION16chain 'P' and (resid 621 through 628 )P621 - 628
17X-RAY DIFFRACTION17chain 'C' and (resid 27 through 36 )C27 - 36
18X-RAY DIFFRACTION18chain 'C' and (resid 37 through 48 )C37 - 48
19X-RAY DIFFRACTION19chain 'C' and (resid 49 through 60 )C49 - 60
20X-RAY DIFFRACTION20chain 'C' and (resid 61 through 76 )C61 - 76
21X-RAY DIFFRACTION21chain 'C' and (resid 77 through 84 )C77 - 84
22X-RAY DIFFRACTION22chain 'C' and (resid 85 through 93 )C85 - 93
23X-RAY DIFFRACTION23chain 'C' and (resid 94 through 100 )C94 - 100
24X-RAY DIFFRACTION24chain 'C' and (resid 101 through 109 )C101 - 109
25X-RAY DIFFRACTION25chain 'D' and (resid 28 through 39 )D28 - 39
26X-RAY DIFFRACTION26chain 'D' and (resid 40 through 59 )D40 - 59
27X-RAY DIFFRACTION27chain 'D' and (resid 60 through 66 )D60 - 66
28X-RAY DIFFRACTION28chain 'D' and (resid 67 through 98 )D67 - 98
29X-RAY DIFFRACTION29chain 'E' and (resid 620 through 624 )E620 - 624
30X-RAY DIFFRACTION30chain 'E' and (resid 625 through 629 )E625 - 629
31X-RAY DIFFRACTION31chain 'E' and (resid 630 through 634 )E630 - 634
32X-RAY DIFFRACTION32chain 'E' and (resid 635 through 637 )E635 - 637
33X-RAY DIFFRACTION33chain 'F' and (resid 611 through 615 )F611 - 615
34X-RAY DIFFRACTION34chain 'F' and (resid 616 through 620 )F616 - 620
35X-RAY DIFFRACTION35chain 'F' and (resid 621 through 628 )F621 - 628

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