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- PDB-7xva: Crystal structure of the Human TR4 Ligand Binding Domain in compl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7xva | ||||||
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Title | Crystal structure of the Human TR4 Ligand Binding Domain in complex with the JAZF1 corepressor fragment | ||||||
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![]() | TRANSCRIPTION / Nuclear receptor / Corepressor / Transcriptional regulation | ||||||
Function / homology | ![]() positive regulation of embryonic development / RNA polymerase II transcription regulatory region sequence-specific DNA binding / fibrillar center / Nuclear Receptor transcription pathway / transcription corepressor activity / nuclear receptor activity / sequence-specific double-stranded DNA binding / nervous system development / spermatogenesis / DNA-binding transcription activator activity, RNA polymerase II-specific ...positive regulation of embryonic development / RNA polymerase II transcription regulatory region sequence-specific DNA binding / fibrillar center / Nuclear Receptor transcription pathway / transcription corepressor activity / nuclear receptor activity / sequence-specific double-stranded DNA binding / nervous system development / spermatogenesis / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / chromatin / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / zinc ion binding / nucleoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, Y. / Chen, Z. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structures of human TR4LBD-JAZF1 and TR4DBD-DNA complexes reveal the molecular basis of transcriptional regulation. Authors: Liu, Y. / Ma, L. / Li, M. / Tian, Z. / Yang, M. / Wu, X. / Wang, X. / Shang, G. / Xie, M. / Chen, Y. / Liu, X. / Jiang, L. / Wu, W. / Xu, C. / Xia, L. / Li, G. / Dai, S. / Chen, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.6 KB | Display | ![]() |
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PDB format | ![]() | 46 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.5 KB | Display | ![]() |
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Full document | ![]() | 435.9 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 16.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7xv6C ![]() 7xv8C ![]() 7xv9C ![]() 3p0uS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28913.188 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: P49116 |
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#2: Protein/peptide | Mass: 2978.276 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: C9JGY8 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 45.98 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: HEPES, ammonium sulfate, sodium acetate, MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 7, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.856→50 Å / Num. obs: 25132 / % possible obs: 98 % / Redundancy: 9.5 % / CC1/2: 0.998 / Net I/σ(I): 36 |
Reflection shell | Resolution: 1.856→1.9 Å / Num. unique obs: 22758 / CC1/2: 0.996 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3P0U Resolution: 1.856→28.09 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.93 / WRfactor Rfree: 0.213 / WRfactor Rwork: 0.191 / SU B: 2.955 / SU ML: 0.086 / Average fsc free: 0.9264 / Average fsc work: 0.9315 / Cross valid method: FREE R-VALUE / ESU R: 0.14 / ESU R Free: 0.125 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.405 Å2
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Refinement step | Cycle: LAST / Resolution: 1.856→28.09 Å
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Refine LS restraints |
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LS refinement shell |
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