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- PDB-7x5g: Nrf2 (A510Y)-MafG heterodimer bound with CsMBE2 -

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Basic information

Entry
Database: PDB / ID: 7x5g
TitleNrf2 (A510Y)-MafG heterodimer bound with CsMBE2
Components
  • DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')
  • DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')
  • Nuclear factor erythroid 2-related factor 2
  • Transcription factor MafG
KeywordsTRANSCRIPTION / Transcription factor / CNC-bZIP / Stress response
Function / homology
Function and homology information


positive regulation of glutathione biosynthetic process / aflatoxin catabolic process / negative regulation of hematopoietic stem cell differentiation / non-membrane-bounded organelle / Regulation of HMOX1 expression and activity / integrated stress response signaling / negative regulation of cellular response to hypoxia / NFE2L2 regulating TCA cycle genes / PERK-mediated unfolded protein response / positive regulation of ERAD pathway ...positive regulation of glutathione biosynthetic process / aflatoxin catabolic process / negative regulation of hematopoietic stem cell differentiation / non-membrane-bounded organelle / Regulation of HMOX1 expression and activity / integrated stress response signaling / negative regulation of cellular response to hypoxia / NFE2L2 regulating TCA cycle genes / PERK-mediated unfolded protein response / positive regulation of ERAD pathway / regulation of cellular response to oxidative stress / negative regulation of vascular associated smooth muscle cell migration / regulation of removal of superoxide radicals / regulation of epidermal cell differentiation / cellular response to laminar fluid shear stress / NFE2L2 regulating ER-stress associated genes / positive regulation of ubiquitin-dependent protein catabolic process / mediator complex / NFE2L2 regulating inflammation associated genes / cellular response to fluid shear stress / NFE2L2 regulates pentose phosphate pathway genes / NFE2L2 regulating MDR associated enzymes / cellular response to angiotensin / Nuclear events mediated by NFE2L2 / Regulation of NFE2L2 gene expression / negative regulation of cardiac muscle cell apoptotic process / NFE2L2 regulating anti-oxidant/detoxification enzymes / NFE2L2 regulating tumorigenic genes / adult behavior / proteasomal ubiquitin-independent protein catabolic process / regulation of innate immune response / regulation of embryonic development / positive regulation of blood vessel endothelial cell migration / positive regulation of blood coagulation / cellular response to glucose starvation / negative regulation of endothelial cell apoptotic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / endoplasmic reticulum unfolded protein response / cellular response to copper ion / molecular condensate scaffold activity / cell redox homeostasis / transcription coregulator binding / response to ischemia / positive regulation of glucose import / protein-DNA complex / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Heme signaling / positive regulation of neuron projection development / cellular response to hydrogen peroxide / RNA polymerase II transcription regulator complex / positive regulation of angiogenesis / positive regulation of reactive oxygen species metabolic process / KEAP1-NFE2L2 pathway / sequence-specific double-stranded DNA binding / cellular response to xenobiotic stimulus / cellular response to tumor necrosis factor / Neddylation / Factors involved in megakaryocyte development and platelet production / regulation of cell population proliferation / cellular response to oxidative stress / cellular response to hypoxia / DNA-binding transcription activator activity, RNA polymerase II-specific / proteasome-mediated ubiquitin-dependent protein catabolic process / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / response to oxidative stress / sequence-specific DNA binding / Potential therapeutics for SARS / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / protein ubiquitination / inflammatory response / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein domain specific binding / centrosome / ubiquitin protein ligase binding / positive regulation of gene expression / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / Golgi apparatus / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Transcription factor Maf family / : / Basic leucine zipper domain, Maf-type / bZIP Maf transcription factor / Transcription factor, Skn-1-like, DNA-binding domain superfamily / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain superfamily / Basic-leucine zipper domain
Similarity search - Domain/homology
DNA / DNA (> 10) / Transcription factor MafG / Nuclear factor erythroid 2-related factor 2
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsSengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. ...Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. / Yamamoto, M. / Ogata, K.
Funding support Japan, 1items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS) Japan
CitationJournal: Nucleic Acids Res. / Year: 2022
Title: Structural basis of transcription regulation by CNC family transcription factor, Nrf2.
Authors: Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Kobayashi, S. / Oguni, A. / Itaya, H. / Kasahara, K. / Moriwaki, H. / Watanabe, C. / Honma, T. / Okada, C. / ...Authors: Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Kobayashi, S. / Oguni, A. / Itaya, H. / Kasahara, K. / Moriwaki, H. / Watanabe, C. / Honma, T. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. / Yamamoto, M. / Ogata, K.
History
DepositionMar 4, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 9, 2022Provider: repository / Type: Initial release
Revision 1.1Dec 14, 2022Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed
Revision 1.2Dec 28, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transcription factor MafG
B: Nuclear factor erythroid 2-related factor 2
C: DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')
D: DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')
E: Transcription factor MafG
F: Nuclear factor erythroid 2-related factor 2
G: DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')
H: DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)70,6289
Polymers70,4348
Non-polymers1941
Water1,946108
1
A: Transcription factor MafG
B: Nuclear factor erythroid 2-related factor 2
C: DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')
D: DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,4115
Polymers35,2174
Non-polymers1941
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: Transcription factor MafG
F: Nuclear factor erythroid 2-related factor 2
G: DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')
H: DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')


Theoretical massNumber of molelcules
Total (without water)35,2174
Polymers35,2174
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)246.022, 55.018, 86.152
Angle α, β, γ (deg.)90.000, 97.300, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
d_1ens_1
d_2ens_1
d_1ens_2
d_2ens_2
d_1ens_3
d_2ens_3
d_1ens_4
d_2ens_4

NCS ensembles :
ID
ens_1
ens_2
ens_3
ens_4

NCS oper:
IDCodeMatrixVector
1given(-0.983723755296, -0.0510355888797, -0.172287381819), (0.179284412846, -0.214593045049, -0.960107767038), (0.0120279913906, -0.97536926017, 0.220250161723)-76.3810578726, -23.3843196369, -43.0123764682
2given(-0.968866173946, -0.141714815053, -0.203015389015), (0.238692714243, -0.316864218008, -0.917944908757), (0.065758080489, -0.937824065878, 0.340825316416)-75.5217629477, -25.8478386347, -44.004614398
3given(-0.96952880105, -0.035050758141, -0.242456899859), (0.236432739954, -0.392990814607, -0.888626906588), (-0.0641362878021, -0.918874128479, 0.389303060094)-73.1347649434, -26.7956009447, -50.9453888484
4given(-0.970663772009, -0.0253384518666, -0.239102079803), (0.231694680386, -0.364343801924, -0.901981800859), (-0.0642605383576, -0.930919737065, 0.35952611359)-73.0284205494, -25.8235092764, -50.4629560784

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Components

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Protein , 2 types, 4 molecules AEBF

#1: Protein Transcription factor MafG / V-maf musculoaponeurotic fibrosarcoma oncogene homolog G / hMAF


Mass: 12071.857 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MAFG / Production host: Escherichia coli (E. coli) / References: UniProt: O15525
#2: Protein Nuclear factor erythroid 2-related factor 2 / NF-E2-related factor 2 / NFE2-related factor 2 / Nrf-2 / HEBP1 / Nuclear factor / erythroid derived 2 / like 2


Mass: 13347.570 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NFE2L2, NRF2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q16236

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DNA chain , 2 types, 4 molecules CGDH

#3: DNA chain DNA (5'-D(*GP*CP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*CP*TP*GP*T)-3')


Mass: 4905.176 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain DNA (5'-D(*CP*AP*CP*AP*GP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*G)-3')


Mass: 4892.194 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 2 types, 109 molecules

#5: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL


Mass: 194.226 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 108 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.3 Å3/Da / Density % sol: 71.36 %
Crystal growTemperature: 298 K / Method: vapor diffusion
Details: 50 mM Tris-HCl (pH 7.5), 100 mM KCl, 10% polyethylene glycol monomethyl ether 550, 2% (v/v) glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-5A / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 3, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.3→50 Å / Num. obs: 50434 / % possible obs: 98.3 % / Redundancy: 4.6 % / Biso Wilson estimate: 53.26 Å2 / Rmerge(I) obs: 0.037 / Net I/σ(I): 34.2
Reflection shellResolution: 2.3→2.34 Å / Redundancy: 4.4 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 2.7 / Num. unique obs: 2456 / % possible all: 96.5

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
HKL-2000data reduction
HKL-2000data scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3A5T

3a5t


Resolution: 2.3→33.89 Å / SU ML: 0.3151 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.271
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2498 5070 10.08 %
Rwork0.2189 45248 -
obs0.222 50318 98.02 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 80.32 Å2
Refinement stepCycle: LAST / Resolution: 2.3→33.89 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3216 1300 13 108 4637
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00254706
X-RAY DIFFRACTIONf_angle_d0.45416583
X-RAY DIFFRACTIONf_chiral_restr0.0266749
X-RAY DIFFRACTIONf_plane_restr0.0023633
X-RAY DIFFRACTIONf_dihedral_angle_d23.96121069
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.330.36841700.3361439X-RAY DIFFRACTION94.81
2.33-2.350.33531670.31311435X-RAY DIFFRACTION96.33
2.35-2.380.3441590.30341531X-RAY DIFFRACTION97.46
2.38-2.410.3231570.29551473X-RAY DIFFRACTION97.6
2.41-2.440.29922020.27861451X-RAY DIFFRACTION97.46
2.44-2.480.3091850.28011494X-RAY DIFFRACTION97.9
2.48-2.510.35981720.28041509X-RAY DIFFRACTION97.79
2.51-2.550.29071690.27861451X-RAY DIFFRACTION97.83
2.55-2.590.30881620.27091515X-RAY DIFFRACTION98.01
2.59-2.630.31331690.27821510X-RAY DIFFRACTION97.96
2.63-2.680.32511450.28031496X-RAY DIFFRACTION98.03
2.68-2.730.32621580.30211506X-RAY DIFFRACTION98.11
2.73-2.780.3181690.30321527X-RAY DIFFRACTION98.43
2.78-2.840.33871840.29571468X-RAY DIFFRACTION98.45
2.84-2.90.30181820.28031521X-RAY DIFFRACTION98.55
2.9-2.960.32881500.26961504X-RAY DIFFRACTION98.39
2.96-3.040.27931710.25031525X-RAY DIFFRACTION98.78
3.04-3.120.24341740.24741496X-RAY DIFFRACTION98.58
3.12-3.210.27311730.2281510X-RAY DIFFRACTION97.45
3.21-3.320.24021880.22251502X-RAY DIFFRACTION98.89
3.32-3.430.24031590.20041524X-RAY DIFFRACTION98.71
3.43-3.570.24461730.20441502X-RAY DIFFRACTION98.65
3.57-3.730.28911790.2191524X-RAY DIFFRACTION99.01
3.73-3.930.2491550.21211574X-RAY DIFFRACTION99.03
3.93-4.180.22881450.17461533X-RAY DIFFRACTION99.06
4.18-4.50.1891840.17471538X-RAY DIFFRACTION99.19
4.5-4.950.22821650.1891539X-RAY DIFFRACTION99.42
4.95-5.660.19571710.18981563X-RAY DIFFRACTION99.26
5.66-7.120.2321630.21871582X-RAY DIFFRACTION99.49
7.13-33.890.20491700.18271506X-RAY DIFFRACTION92.19
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.30600730378-0.837648094628-1.910587835352.947491649910.5957171793233.157047040960.203219358981-0.4064395259030.540717745795-0.286088680263-0.0323865527552-0.120645966175-0.262960843780.420008363085-0.1847810421610.402698187255-0.0424232363736-0.04450476783060.3233380620060.07271731258820.369173877301-54.2568167101-30.5197804077-14.4975075725
21.13142026745-1.815260143272.904648273346.99158186739-5.653696719577.84977081024-0.388616857816-0.294704874571-0.679575194524-1.20872845568-0.299534558095-0.6668435069830.9220643133770.8108131262470.6511449433390.8440797690570.06542820364450.3288921231850.8501739914270.4537962072780.991881978357-43.062832964-61.6071388956-9.6248722977
34.469043454573.78078889148-1.43295392944.69183318427-4.59858042968.42737582761-0.908035608411-0.857614405818-1.44635987619-0.714306711702-0.649214122821-1.055832983680.7783616743411.568583097811.481852097870.60807296230.130003943150.1411617787410.5612980548550.3293879565530.823569968415-36.3197824698-46.5260916664-17.2297012803
49.216141743671.11702291356.922679457676.17789668381-1.283771171556.51545555962-0.2924327351512.07042585622-0.848387709796-1.46581356223-0.0858011652828-0.4320511219620.5877857407250.973791015340.4426423138820.9518532772950.09362699026620.3306220867421.212884982260.09346649916060.73284175798-36.7359222197-43.3152567412-35.0260951245
52.70792611152-3.875365635311.49488827969.61553137105-3.902150047792.057484410851.452601280620.3443675592181.41734951814-0.87260269405-0.4347626507981.169230169951.37244144591-0.409135913358-1.085979835850.9330270887080.246247314540.2366794602261.214912335510.4366256980951.05634492768-39.5503748929-30.7635667869-38.0206087286
65.638708186874.79247853935-5.626403377096.48126185075-2.002356783078.669610616660.9054966249160.8874531217722.37507172685-0.878289180628-0.222806534206-0.0175992918363-0.1088680306212.02919178753-0.5444407485220.949098855905-0.01489971632940.151594616721.205036598210.4094108171290.740759722734-31.7648649791-32.9390480864-33.4245615306
76.53181382658-0.291690545111-1.014488000573.60321176463-2.702960564982.8225967242-0.4428786109310.0576602313451-0.767938261684-0.316980777943-0.931764878514-1.661333229251.027631949581.048299243611.407879292360.6976829464170.05194169633240.12935233510.4906312826820.1865451978380.966970911916-38.9619322696-51.7453971666-16.4912511153
89.9328251223-5.38209619807-2.490081710897.185840632421.330057056147.834608860560.619608563272-2.422393221010.3697546486711.939278495480.0922256476610.423896501473-0.9555665412241.75661213452-0.6630948519731.14192920605-0.2505014509840.2497070727281.1759778423-0.1892522293480.88018202628-42.870477182-10.578592036946.7405421398
99.48960561206-3.3721821077-8.145124737532.15973432353.167848524046.59964490262-0.240186346267-0.5596336249930.2087855904630.2611758500910.336510973876-0.3122073167450.3199009148740.329719733932-0.1742731613760.3845168300790.0570966730812-0.07640572455160.7802198428110.009202439421440.466313939231-12.433124369-26.576253454415.5166269238
108.45924009868-0.138703862868-3.752761243994.676489963972.335240450017.43696972619-0.1829005582660.508808695944-0.72823979888-0.181950553645-0.1609652839530.2597908164750.568360459085-0.65797518760.3256618031810.332315061532-0.0154867825441-0.01062479906450.322637483595-0.01952313509820.478320987172-60.2746369115-20.43359291624.5381182353
119.43708264339-1.87052024986-0.3763663344290.987337694188-0.05219071082790.762666929195-0.0172549600728-0.452631954185-1.379966807420.0211598878938-0.02146561787870.1074169740360.07561748184460.491285522788-0.01150674696170.3922856388760.07465622929330.03897994445510.5865728175610.1191540568710.552679952793-21.340668053-28.965658254610.4213665659
120.3761434381740.211342621573-0.002891268900240.684430439246-0.6519554965758.144109848280.193723483698-0.950789130694-0.5948788393250.511996592140.1634841800710.177184012530.5390909956211.1248932345-0.2748385657850.6825654986810.1509396375980.109740084580.915877204120.2509510352360.498279645419-41.2368240444-24.885856603930.4264154808
136.913273933754.48200171550.001138676685793.82830123403-1.443912923522.64670795090.1059445749490.6663598082360.872755223549-0.388911150550.299548822327-0.385920152993-0.3328216032180.763144155213-0.5086474668180.733537895987-0.0312758451380.09237299401240.6405248863380.1088190202960.625219726876-39.3707586414-9.835634085710.0944508669
145.90416798456-4.837569432254.291005745759.20626060509-4.715058356795.567334302980.4909288854180.511737186076-0.276129398681-0.7882438128960.4022647852471.177967531230.598234982569-0.44032201213-0.7519308540420.850703485875-0.1591929114020.02435639099120.6502364743810.1804597894540.557662478584-42.7130047068-14.52485405542.8615610814
157.354852831280.319580926334-0.1606503292617.69195394447-3.716570141077.81425806281-0.118332683111-0.621979807798-0.2539935537190.7060541343180.4876575080450.6497240749020.5437442251060.198222616078-0.287260432860.5658075604210.06950816534020.07130502587180.5527982889290.06034351845520.435994086842-41.2790425288-22.964691461327.3941135337
160.2958737617130.683752813934-0.450024227611.51649438101-0.9125029582280.723943323377-0.06273690210050.121767378257-0.904671399162-1.18311697037-0.0411885912851-0.08544072247980.915017578917-0.0380320691829-0.009017903906681.355526708010.05772121239030.06800392824950.610641426534-0.1166651990871.87020028817-45.5407244138-73.0713270234-21.610219896
173.45897222034-4.395551876020.5357527125195.68857529286-1.256496982333.312356066630.3994819401520.2467209411290.696632542898-1.50916709501-0.402305839512-1.00585877894-0.1006325969410.4528242105840.03485839086451.872662908990.2315342995040.4533452248580.724987832071-0.1762827119473.05990392443-30.8477449197-77.3068036698-18.1958432766
184.80086629221-0.888024997736-1.152861493070.974829497222-0.118407805791.627292250670.2506616688130.564901669815-0.180437903271-0.341214685104-0.4569879729350.3901508246540.0224136197128-0.3049097162790.1654867960350.315861683234-0.0711565609662-0.03401120693540.376212930145-0.02070383254730.459318513022-65.5362905057-34.26439115-14.1826583348
195.08405206988-2.67967244826-4.629393002383.659040042751.439358922777.99665673315-0.597324787324-0.234020887971-0.412032119225-0.0915877511287-0.0701639363105-1.67560375329-0.1405065067332.251142935450.6542607825120.710683470926-0.1367122835160.2028285422461.739464534550.1707491968311.32356532728-15.0534868697-38.2752389899-27.2486166559
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'F' and (resid 492 through 558 )FG492 - 55838 - 104
22chain 'G' and (resid 0 through 4 )GH0 - 4
33chain 'G' and (resid 5 through 9 )GH5 - 9
44chain 'G' and (resid 10 through 14 )GH10 - 14
55chain 'G' and (resid 15 through 15 )GH15
66chain 'H' and (resid 0 through 4 )HI0 - 4
77chain 'H' and (resid 5 through 15 )HI5 - 15
88chain 'A' and (resid 24 through 45 )AA24 - 451 - 22
99chain 'A' and (resid 46 through 122 )AA46 - 12223 - 99
1010chain 'B' and (resid 454 through 491 )BB454 - 4911 - 38
1111chain 'B' and (resid 492 through 557 )BB492 - 55739 - 104
1212chain 'C' and (resid 0 through 9 )CC0 - 9
1313chain 'C' and (resid 10 through 15 )CC10 - 15
1414chain 'D' and (resid 0 through 4 )DE0 - 4
1515chain 'D' and (resid 5 through 15 )DE5 - 15
1616chain 'E' and (resid 27 through 41 )EF27 - 411 - 15
1717chain 'E' and (resid 42 through 47 )EF42 - 4716 - 21
1818chain 'E' and (resid 48 through 121 )EF48 - 12122 - 95
1919chain 'F' and (resid 455 through 491 )FG455 - 4911 - 37

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