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- PDB-7x5e: Nrf2-MafG heterodimer bound with CsMBE1 -

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Basic information

Entry
Database: PDB / ID: 7x5e
TitleNrf2-MafG heterodimer bound with CsMBE1
Components
  • DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')
  • DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')
  • Nuclear factor erythroid 2-related factor 2
  • Transcription factor MafG
KeywordsTRANSCRIPTION/DNA / Transcription factor / CNC-bZIP / Stress response / TRANSCRIPTION / TRANSCRIPTION-DNA complex
Function / homology
Function and homology information


positive regulation of glutathione biosynthetic process / aflatoxin catabolic process / negative regulation of hematopoietic stem cell differentiation / integrated stress response signaling / Regulation of HMOX1 expression and activity / negative regulation of cellular response to hypoxia / NFE2L2 regulating TCA cycle genes / PERK-mediated unfolded protein response / positive regulation of ERAD pathway / regulation of removal of superoxide radicals ...positive regulation of glutathione biosynthetic process / aflatoxin catabolic process / negative regulation of hematopoietic stem cell differentiation / integrated stress response signaling / Regulation of HMOX1 expression and activity / negative regulation of cellular response to hypoxia / NFE2L2 regulating TCA cycle genes / PERK-mediated unfolded protein response / positive regulation of ERAD pathway / regulation of removal of superoxide radicals / regulation of cellular response to oxidative stress / cellular response to laminar fluid shear stress / regulation of epidermal cell differentiation / NFE2L2 regulating ER-stress associated genes / NFE2L2 regulating inflammation associated genes / mediator complex / cellular response to fluid shear stress / cellular response to methionine / NFE2L2 regulates pentose phosphate pathway genes / NFE2L2 regulating MDR associated enzymes / positive regulation of ubiquitin-dependent protein catabolic process / Nuclear events mediated by NFE2L2 / Regulation of NFE2L2 gene expression / negative regulation of cardiac muscle cell apoptotic process / NFE2L2 regulating tumorigenic genes / NFE2L2 regulating anti-oxidant/detoxification enzymes / negative regulation of ferroptosis / proteasomal ubiquitin-independent protein catabolic process / regulation of innate immune response / negative regulation of vascular associated smooth muscle cell migration / adult behavior / cellular response to angiotensin / regulation of embryonic development / positive regulation of blood coagulation / positive regulation of blood vessel endothelial cell migration / negative regulation of endothelial cell apoptotic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / endoplasmic reticulum unfolded protein response / cellular response to glucose starvation / reactive oxygen species metabolic process / cell redox homeostasis / cellular response to copper ion / response to ischemia / transcription coregulator binding / positive regulation of D-glucose import / molecular condensate scaffold activity / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Heme signaling / protein-DNA complex / positive regulation of neuron projection development / RNA polymerase II transcription regulator complex / cellular response to xenobiotic stimulus / cellular response to hydrogen peroxide / positive regulation of reactive oxygen species metabolic process / positive regulation of angiogenesis / sequence-specific double-stranded DNA binding / cellular response to tumor necrosis factor / KEAP1-NFE2L2 pathway / regulation of cell population proliferation / Neddylation / Factors involved in megakaryocyte development and platelet production / response to oxidative stress / cellular response to oxidative stress / DNA-binding transcription activator activity, RNA polymerase II-specific / cellular response to hypoxia / sequence-specific DNA binding / in utero embryonic development / Potential therapeutics for SARS / proteasome-mediated ubiquitin-dependent protein catabolic process / RNA polymerase II-specific DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription cis-regulatory region binding / protein ubiquitination / ciliary basal body / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / inflammatory response / protein domain specific binding / ubiquitin protein ligase binding / centrosome / positive regulation of gene expression / regulation of transcription by RNA polymerase II / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / Golgi apparatus / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Transcription factor Maf family / : / Basic leucine zipper domain, Maf-type / bZIP Maf transcription factor / Transcription factor, Skn-1-like, DNA-binding domain superfamily / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain / Basic-leucine zipper domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / Transcription factor MafG / Nuclear factor erythroid 2-related factor 2
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsSengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. ...Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. / Yamamoto, M. / Ogata, K.
Funding support Japan, 1items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS) Japan
CitationJournal: Nucleic Acids Res. / Year: 2022
Title: Structural basis of transcription regulation by CNC family transcription factor, Nrf2.
Authors: Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Kobayashi, S. / Oguni, A. / Itaya, H. / Kasahara, K. / Moriwaki, H. / Watanabe, C. / Honma, T. / Okada, C. / ...Authors: Sengoku, T. / Shiina, M. / Suzuki, K. / Hamada, K. / Sato, K. / Uchiyama, A. / Kobayashi, S. / Oguni, A. / Itaya, H. / Kasahara, K. / Moriwaki, H. / Watanabe, C. / Honma, T. / Okada, C. / Baba, S. / Ohta, T. / Motohashi, H. / Yamamoto, M. / Ogata, K.
History
DepositionMar 4, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 9, 2022Provider: repository / Type: Initial release
Revision 1.1Dec 14, 2022Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Dec 28, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transcription factor MafG
B: Nuclear factor erythroid 2-related factor 2
C: DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')
D: DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')
E: Transcription factor MafG
F: Nuclear factor erythroid 2-related factor 2
G: DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')
H: DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)70,5289
Polymers70,2458
Non-polymers2821
Water3,027168
1
A: Transcription factor MafG
B: Nuclear factor erythroid 2-related factor 2
C: DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')
D: DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')


Theoretical massNumber of molelcules
Total (without water)35,1234
Polymers35,1234
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6750 Å2
ΔGint-56 kcal/mol
Surface area18760 Å2
MethodPISA
2
E: Transcription factor MafG
F: Nuclear factor erythroid 2-related factor 2
G: DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')
H: DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,4055
Polymers35,1234
Non-polymers2821
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6320 Å2
ΔGint-50 kcal/mol
Surface area19400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)257.547, 54.840, 82.274
Angle α, β, γ (deg.)90.000, 96.770, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y

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Components

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Protein , 2 types, 4 molecules AEBF

#1: Protein Transcription factor MafG / V-maf musculoaponeurotic fibrosarcoma oncogene homolog G / hMAF


Mass: 12071.857 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MAFG / Production host: Escherichia coli (E. coli) / References: UniProt: O15525
#2: Protein Nuclear factor erythroid 2-related factor 2 / NF-E2-related factor 2 / NFE2-related factor 2 / Nrf-2 / HEBP1 / Nuclear factor / erythroid derived 2 / like 2


Mass: 13255.474 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NRF2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q16236

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DNA chain , 2 types, 4 molecules CGDH

#3: DNA chain DNA (5'-D(*GP*TP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*T)-3')


Mass: 4864.164 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain DNA (5'-D(*CP*AP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*A)-3')


Mass: 4931.230 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 2 types, 169 molecules

#5: Chemical ChemComp-P6G / HEXAETHYLENE GLYCOL / POLYETHYLENE GLYCOL PEG400


Mass: 282.331 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C12H26O7 / Comment: precipitant*YM
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 168 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.11 Å3/Da / Density % sol: 70.05 %
Crystal growTemperature: 298 K / Method: vapor diffusion
Details: 50 mM MES monohydrate (pH 5.6), 20 mM KCl, 10 mM MgSO4 heptahydrate, 7%(v/v) PEG400

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jan 31, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.3→50 Å / Num. obs: 50732 / % possible obs: 99.2 % / Redundancy: 3.1 % / Biso Wilson estimate: 43.4 Å2 / Rsym value: 0.075 / Net I/σ(I): 12.2
Reflection shellResolution: 2.3→2.38 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.43 / Mean I/σ(I) obs: 2.5 / Num. unique obs: 5033 / % possible all: 99.8

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
HKL-2000data reduction
SCALEPACKdata scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3A5T

3a5t


Resolution: 2.3→40.85 Å / SU ML: 0.3155 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 27.3127
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2418 5109 10.09 %
Rwork0.2117 45538 -
obs0.2149 50647 98.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 58.35 Å2
Refinement stepCycle: LAST / Resolution: 2.3→40.85 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3279 1300 19 168 4766
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00344792
X-RAY DIFFRACTIONf_angle_d0.52576700
X-RAY DIFFRACTIONf_chiral_restr0.0297769
X-RAY DIFFRACTIONf_plane_restr0.0038645
X-RAY DIFFRACTIONf_dihedral_angle_d23.58461085
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.330.38781530.29871407X-RAY DIFFRACTION94.95
2.33-2.350.35041990.29331526X-RAY DIFFRACTION99.37
2.35-2.380.36381830.2641488X-RAY DIFFRACTION99.23
2.38-2.410.30061700.26051516X-RAY DIFFRACTION99.53
2.41-2.440.27921600.24341502X-RAY DIFFRACTION99.64
2.44-2.480.28291790.24221559X-RAY DIFFRACTION99.66
2.48-2.510.31411580.24611479X-RAY DIFFRACTION99.7
2.51-2.550.29971660.24811521X-RAY DIFFRACTION99.59
2.55-2.590.27081630.2291562X-RAY DIFFRACTION99.48
2.59-2.630.26621740.24121499X-RAY DIFFRACTION99.88
2.63-2.680.30991620.25541506X-RAY DIFFRACTION99.76
2.68-2.730.29161860.2471539X-RAY DIFFRACTION99.71
2.73-2.780.31161350.26511532X-RAY DIFFRACTION99.58
2.78-2.840.3191720.25841524X-RAY DIFFRACTION99.88
2.84-2.90.30941620.27651528X-RAY DIFFRACTION99.71
2.9-2.970.32731730.28621502X-RAY DIFFRACTION99.82
2.97-3.040.30732010.26631523X-RAY DIFFRACTION99.71
3.04-3.120.28651800.2321500X-RAY DIFFRACTION99.64
3.12-3.210.26681720.23241534X-RAY DIFFRACTION99.48
3.21-3.320.23841820.22261495X-RAY DIFFRACTION99.23
3.32-3.440.23991660.21491537X-RAY DIFFRACTION99.24
3.44-3.570.2431630.2161514X-RAY DIFFRACTION99.35
3.57-3.740.22491710.20551522X-RAY DIFFRACTION99.06
3.74-3.930.22071580.19961557X-RAY DIFFRACTION98.56
3.93-4.180.18861750.17781510X-RAY DIFFRACTION98.88
4.18-4.50.20391730.17131509X-RAY DIFFRACTION97.73
4.5-4.950.21681520.1771561X-RAY DIFFRACTION98.79
4.95-5.670.21921770.18481504X-RAY DIFFRACTION98.13
5.67-7.130.21131620.20591551X-RAY DIFFRACTION97.89
7.14-40.850.16411820.15171531X-RAY DIFFRACTION94.07
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.10755873885-0.187324824718-1.749770407971.216875912540.1460215315242.673149084270.880286206694-0.9122797518080.2569728594580.596488675272-0.265989216895-0.315536803855-0.9084231411931.340337772390.008855716832130.631325793155-0.04715596787890.1562090169820.872626766361-0.1353313072850.589742736628-44.2645882188-8.0445315956644.4462444494
23.7305427951-1.02456102187-3.198524445210.5781450576891.174852257622.26281241858-0.251679879252-0.3285716705480.1169260146390.2197286874630.269769807053-0.136756047970.261817267180.214485472-0.06206482434220.2904414115710.093271565762-0.02604131713630.6648750997890.005712794121550.366550854896-13.2161332858-24.915104666315.417417
33.2803313121-0.257315124419-0.4708055147242.68965401822-0.1069173000412.08514745601-0.2888508692460.446932464643-0.736544180028-0.342811642434-0.06111804483810.06633372162170.547577921418-0.1125895199830.05031499231590.3125982479690.023180250589-0.03583489218130.254501861105-0.02584731819120.395145152021-60.4089599713-20.56795546272.66291724304
43.97186709563-0.373514439205-0.1845572337160.1832693314850.09131626455320.214853306985-0.0282805461929-0.0408803364948-0.616891516632-0.04340315085890.03262055131860.08532974350720.003401910303930.298818359152-0.04764643833290.3668557216850.0511912742053-0.008857545351580.5171966601190.05408094247270.428547094598-21.6035407858-27.67698217629.91853843974
50.449950413836-0.0871899955169-0.7379245981522.176927986550.3132063820532.18797174666-0.23743427162-0.526103362462-0.1316374104140.2003864893260.4493361286220.6092873512660.6829621746220.07601209155170.0481054180030.5718439142730.2689556047090.1229264494480.6913531052670.2850098417220.341933222357-41.1154054061-23.880464535738.601985267
61.96306379134-0.5936304904152.189504069690.71702449467-0.7565975036713.307617395860.116799099481-0.122363140719-0.9710556713580.06028209891870.3763845681640.3510024771411.001131309481.02552898055-0.05120967609330.6541651943840.120722677534-0.01386750564720.4632949300760.09250212621690.463964888488-43.2618412247-23.617045080820.850493034
74.277671558861.02712999957-1.283739726133.212540473710.7757697698366.0227906252-0.4349189275710.8578863275260.756002465353-1.469295574140.437778562816-0.249568991624-0.3799362662480.8228895044240.08616944633770.595597955947-0.116719288896-0.04094384072260.3692966137850.2000166877680.534820044832-40.9655119467-8.8802749916210.3519649876
83.278559795330.270704713801-1.420416016510.425913772837-0.6432903095531.30017109144-0.0736422875608-0.0405689166358-0.0526803480318-0.210381991650.0298800638003-0.0957041699042-0.1298746908220.2992332664830.2278107604061.29196063659-0.2266969701120.3963647060291.027947572710.2364357066080.355778226641-36.1344264199-10.6489753582-1.49762888755
90.723757305697-0.1892049689090.9715171429061.3929586993-0.5336772073671.365695225550.158900691345-0.2112620304370.6574931173380.3038901414540.2839214466770.750894361126-1.21128267253-0.220820417504-0.06037219827310.603218125130.04923078829550.1504865799140.3705739715070.1183190245740.406178081688-43.8299747045-14.67845994842.09112198139
101.816622185830.4801172332680.1823958690851.25577790947-0.8252074744481.643544018-0.210097279548-0.677672920381-0.2075176732840.7341941423470.5961570012920.8192672548680.5881102235360.2255805871920.0980131900190.6593538667020.1393436501850.08276124099790.5297717912480.1817270084750.557324833059-42.2135295326-21.719527711326.1737300634
111.46613389576-0.9624879895060.910042702652.07273034108-0.3866029652871.542856219410.1874609066550.178305054281-1.579634807740.037036257096-0.2021077036410.07738854743230.77817704282-0.4635442029760.09088208879550.6906313218090.04084541665930.09209558326640.534382189958-0.1360343970830.99927582978-44.9849201388-71.633780758-24.0624483484
123.33903772517-2.1753909967-0.8048913193142.609084334950.8908739376020.701966197120.1167804104950.3076247667180.170319039015-0.149260619723-0.153487655153-0.02948004035360.0416984877281-0.2061118014920.1508898677490.3470654190060.0116418281913-0.02371098493010.3306675549070.01277321829620.361099206831-69.2429143336-34.1140645554-13.4708672298
130.2818167815980.401005287902-0.377371963882.521598603760.9736961742941.842885269870.1629827250140.2851892529090.781419065940.18997698794-0.0623405864541-1.207630624210.06973134584531.44450599205-0.03650922639160.4150219425930.006299033999490.08551221496090.9547332801830.1216041452990.914148511802-20.6468918816-34.8975597072-24.326223968
144.59204834578-1.15995638962-0.8392714936981.018000997480.04351712147111.17225451195-0.0961560050997-0.105752920530.165734425133-0.09046546697010.0230762290238-0.0158290497117-0.0217341632853-0.004741608045710.1007375899350.373927875136-0.0253427561525-0.03882632915290.229261587396-0.02096757621360.425570416901-60.9052761085-28.3469930166-11.8545937489
151.925599843020.740622489581-1.205424404510.980846641266-0.5936340483861.358789768330.0911353667103-0.04423734105170.0198671564120.138983757936-0.04950436090490.2735737239030.1925069671070.2390126308910.08297883852450.5004418739320.04074385002140.1150612332690.3525231419030.06353015733910.408886586528-43.8620439695-52.2906174431-12.5402440015
163.57576384032-2.554345119864.379575816574.80147154297-1.251270616836.547421975680.131694515021.302383886720.682173535788-1.510457183950.2509537886440.162468785057-0.06792018182110.4426889917380.6769963704850.620714332418-0.0295371764911-0.02939375499320.8878459830990.2511113126290.391375647725-41.9079577043-39.4895635185-32.6320443939
171.08371960413-0.9041010303920.9805612005890.811022512769-0.5798135382022.08565993471-0.178403996661-0.03265622334950.0956059924442-0.10551549120.1400238062630.178584806069-0.146540984951-0.246101807124-1.214120324311.21414971493-0.0294845471091-0.01231338990930.9916811315710.737885583970.739869429275-43.6256928903-27.2375198878-35.8515395884
180.5882662948790.468330958942-0.664984100351.210293529250.8310678277982.99191890098-0.007582579712070.1405835788830.417608969059-0.6403413818250.3189774846910.547944825115-0.01645558015410.4328773850950.2480649219310.6908706396320.08530218102940.1586679579680.8173803726840.3922517405480.605159514731-36.6096507567-30.62849176-30.5180414475
195.541878915931.522507294050.8905573809114.04829148061-0.3313991789050.222637461274-0.2302737183980.8131106934551.55668731418-0.1254253514710.3059886210320.553367247261-0.01196088920380.0358858952136-0.04243032290730.492317475069-0.01590939850280.06677261314140.5004221657640.1534053868230.449062728143-45.5991094068-44.5467763784-24.535112551
201.202686441980.0197525644092-0.4635070466451.48420193748-0.4719200039993.916285739430.03203941890370.01746564243290.0981103328995-0.045957054841-0.01707391607480.05747641925880.4170390848071.119133303120.09099595111370.518741002401-0.01045673384590.04844885016520.5628456923420.09167955482940.435629653185-41.3125975003-53.6074714377-7.81468327609
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 23 through 45 )AA23 - 451 - 23
22chain 'A' and (resid 46 through 121 )AA46 - 12124 - 99
33chain 'B' and (resid 453 through 491 )BB453 - 4911 - 39
44chain 'B' and (resid 492 through 558 )BB492 - 55840 - 106
55chain 'C' and (resid 0 through 4 )CC0 - 4
66chain 'C' and (resid 5 through 9 )CC5 - 9
77chain 'C' and (resid 10 through 14 )CC10 - 14
88chain 'C' and (resid 15 through 15 )CC15
99chain 'D' and (resid 0 through 4 )DD0 - 4
1010chain 'D' and (resid 5 through 15 )DD5 - 15
1111chain 'E' and (resid 21 through 45 )EE21 - 451 - 25
1212chain 'E' and (resid 46 through 121 )EE46 - 12126 - 101
1313chain 'F' and (resid 455 through 491 )FG455 - 4911 - 37
1414chain 'F' and (resid 492 through 559 )FG492 - 55938 - 105
1515chain 'G' and (resid 0 through 9 )GH0 - 9
1616chain 'G' and (resid 10 through 14 )GH10 - 14
1717chain 'G' and (resid 15 through 15 )GH15
1818chain 'H' and (resid 0 through 4 )HI0 - 4
1919chain 'H' and (resid 5 through 9 )HI5 - 9
2020chain 'H' and (resid 10 through 15 )HI10 - 15

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