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- PDB-7wl0: Crystal structure of human ALKBH5 in complex with N-oxalylglycine... -

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Basic information

Entry
Database: PDB / ID: 7wl0
TitleCrystal structure of human ALKBH5 in complex with N-oxalylglycine (NOG) and m6A-containing ssRNA
Components
  • RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
  • RNA demethylase ALKBH5
KeywordsOXIDOREDUCTASE/RNA / Iron and 2-oxoglutarate dependent oxygenase / RNA demethylase / oxidoreductase / double-stranded beta helix / OXIDOREDUCTASE-RNA COMPLEX
Function / homology
Function and homology information


gamma-delta T cell proliferation / Reversal of alkylation damage by DNA dioxygenases / mRNA N6-methyladenine demethylase / mRNA N6-methyladenosine dioxygenase activity / oxidative RNA demethylase activity / regulation of mRNA export from nucleus / regulation of mRNA processing / 2-oxoglutarate-dependent dioxygenase activity / membraneless organelle assembly / paraspeckles ...gamma-delta T cell proliferation / Reversal of alkylation damage by DNA dioxygenases / mRNA N6-methyladenine demethylase / mRNA N6-methyladenosine dioxygenase activity / oxidative RNA demethylase activity / regulation of mRNA export from nucleus / regulation of mRNA processing / 2-oxoglutarate-dependent dioxygenase activity / membraneless organelle assembly / paraspeckles / mRNA destabilization / molecular condensate scaffold activity / mRNA processing / regulation of translation / spermatogenesis / cell differentiation / response to hypoxia / nuclear speck / Golgi apparatus / RNA binding / nucleoplasm / metal ion binding / nucleus / cytosol
Similarity search - Function
RNA demethylase ALKBH5 / Alpha-ketoglutarate-dependent dioxygenase AlkB-like / 2OG-Fe(II) oxygenase superfamily / Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily
Similarity search - Domain/homology
FORMIC ACID / : / N-OXALYLGLYCINE / RNA / RNA demethylase ALKBH5
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsKaur, S. / McDonough, M.A. / Schofield, C.J. / Aik, W.S.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
The University Grants Committee, Research Grants Council (RGC)22301719 United Kingdom
Wellcome Trust106244/Z/14/Z United Kingdom
CitationJournal: Nucleic Acids Res. / Year: 2022
Title: Mechanisms of substrate recognition and N6-methyladenosine demethylation revealed by crystal structures of ALKBH5-RNA complexes.
Authors: Kaur, S. / Tam, N.Y. / McDonough, M.A. / Schofield, C.J. / Aik, W.S.
History
DepositionJan 12, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Mar 30, 2022Provider: repository / Type: Initial release
Revision 1.1Apr 6, 2022Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation_author.identifier_ORCID
Revision 1.2May 4, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: RNA demethylase ALKBH5
B: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
C: RNA demethylase ALKBH5
D: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
E: RNA demethylase ALKBH5
F: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
G: RNA demethylase ALKBH5
H: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
I: RNA demethylase ALKBH5
J: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,53321
Polymers138,47710
Non-polymers1,05611
Water3,153175
1
A: RNA demethylase ALKBH5
B: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,8974
Polymers27,6952
Non-polymers2022
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2660 Å2
ΔGint-14 kcal/mol
Surface area11030 Å2
MethodPISA
2
C: RNA demethylase ALKBH5
D: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,9435
Polymers27,6952
Non-polymers2483
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2500 Å2
ΔGint-13 kcal/mol
Surface area11050 Å2
MethodPISA
3
E: RNA demethylase ALKBH5
F: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,8974
Polymers27,6952
Non-polymers2022
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2720 Å2
ΔGint-16 kcal/mol
Surface area11090 Å2
MethodPISA
4
G: RNA demethylase ALKBH5
H: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,8974
Polymers27,6952
Non-polymers2022
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2430 Å2
ΔGint-12 kcal/mol
Surface area10580 Å2
MethodPISA
5
I: RNA demethylase ALKBH5
J: RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,8974
Polymers27,6952
Non-polymers2022
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2500 Å2
ΔGint-12 kcal/mol
Surface area10390 Å2
MethodPISA
Unit cell
Length a, b, c (Å)59.770, 132.370, 77.960
Angle α, β, γ (deg.)90.000, 100.990, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Protein / RNA chain , 2 types, 10 molecules ACEGIBDFHJ

#1: Protein
RNA demethylase ALKBH5 / Alkylated DNA repair protein alkB homolog 5 / Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5


Mass: 25138.818 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ALKBH5, ABH5, OFOXD1 / Production host: Escherichia coli (E. coli)
References: UniProt: Q6P6C2, mRNA N6-methyladenine demethylase
#2: RNA chain
RNA (5'-R(*UP*GP*GP*(6MZ)P*CP*UP*GP*C)-3')


Mass: 2556.588 Da / Num. of mol.: 5 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 4 types, 186 molecules

#3: Chemical
ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mn / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical
ChemComp-OGA / N-OXALYLGLYCINE


Mass: 147.086 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C4H5NO5 / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: CH2O2
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 175 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.19 Å3/Da / Density % sol: 43.74 %
Description: THE ENTRY CONTAINS FRIEDEL PAIRS IN I/F_PLUS/MINUS COLUMNS.
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 0.2 M sodium sulfate decahydrate, 20% (w/v) PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9999 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 13, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9999 Å / Relative weight: 1
ReflectionResolution: 2.5→66.25 Å / Num. obs: 41144 / % possible obs: 99.92 % / Redundancy: 14 % / Biso Wilson estimate: 42.4 Å2 / CC1/2: 0.981 / Rmerge(I) obs: 0.4891 / Net I/σ(I): 4.76
Reflection shellResolution: 2.5→2.59 Å / Redundancy: 11.8 % / Rmerge(I) obs: 2.437 / Mean I/σ(I) obs: 1.17 / Num. unique obs: 4103 / CC1/2: 0.42 / % possible all: 99.81

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Processing

Software
NameVersionClassification
xia2data scaling
PHENIX1.18.2_3874refinement
PDB_EXTRACT3.27data extraction
xia2data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7V4G

7v4g


Resolution: 2.5→58.67 Å / SU ML: 0.4 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 27.47 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2404 2066 5.02 %
Rwork0.1963 39051 -
obs0.1985 41117 99.93 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 142.09 Å2 / Biso mean: 49.1942 Å2 / Biso min: 28.12 Å2
Refinement stepCycle: final / Resolution: 2.5→58.67 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8507 622 58 175 9362
Biso mean--47.81 42.12 -
Num. residues----1114
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 15 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
2.5-2.560.37461430.309125742717
2.56-2.620.34411540.293425822736
2.62-2.690.3481550.273925792734
2.69-2.770.31161250.263225872712
2.77-2.860.31231180.274926172735
2.86-2.960.33661270.249126282755
2.96-3.080.27741440.245425702714
3.08-3.220.31661310.224625892720
3.22-3.390.27941350.198626222757
3.39-3.610.23421250.200826022727
3.61-3.880.23481340.172526232757
3.88-4.270.21621200.161726492769
4.27-4.890.17131590.140625732732
4.89-6.160.18831530.169926082761
6.16-58.670.18961430.164426482791
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.3414-2.80651.67536.0057-3.29595.2688-0.3658-0.42210.08260.76130.44210.2348-0.1015-0.6055-0.22130.3889-0.05420.15770.52020.01240.3411-4.3133-34.098140.5965
24.63011.6157-5.86234.2025-1.53247.3685-0.290.34980.5634-0.43990.27680.238-0.086-0.04210.07790.4618-0.0232-0.09910.4086-0.02060.3587-5.4667-20.694717.9791
33.51020.23571.03020.44140.45112.58690.02450.2624-0.19520.12720.09820.02680.01270.3094-0.10320.3751-0.01130.07890.3768-0.00960.376113.9029-24.715522.2014
45.9809-1.3652-0.67032.35252.41253.24110.0936-0.09470.16140.0291-0.18560.1719-0.1579-0.370.040.38640.01210.0540.2639-0.00460.3647-0.9541-15.097231.566
52.3724-0.75950.93341.4343-0.04880.95360.051-0.0485-0.325-0.0703-0.06250.17720.2589-0.09420.00610.3827-0.0320.10580.3542-0.02240.34233.2463-34.616629.5841
63.1223-0.59510.08212.82841.55366.0407-0.1142-0.2652-0.4488-0.06350.1683-0.1165-0.47610.3374-0.11180.3077-0.00660.08240.47720.02310.41145.9808-36.640537.4232
73.52111.99991.34453.96621.65532.80880.0881-0.16480.32630.4168-0.22750.42140.1379-0.12530.18290.27440.04880.10240.271-0.00440.29965.8814-27.461928.4949
88.287-1.3609-0.1946.01443.14791.69970.03480.0247-0.71640.40290.2623-0.4881.1320.3486-0.51920.6950.05060.03630.3655-0.01390.384514.3552-40.796826.5173
97.34951.14644.33481.6106-0.09993.9989-0.0901-0.1866-0.4286-0.12640.2304-0.14210.1835-0.1162-0.11980.39080.06740.15940.4584-0.0530.4314-39.1222-25.739755.3826
104.0352-0.8701-5.59136.51871.12888.36880.3117-0.71980.66350.0526-0.1697-0.4827-0.67960.8324-0.11130.3309-0.0238-0.02070.3686-0.01570.3712-11.3605-16.673764.4843
116.1412-0.32572.03736.7758-3.58264.2981-0.05590.36650.5168-0.670.1442-0.66770.38790.0238-0.05190.3041-0.01430.11360.3362-0.01630.3523-7.267-23.775950.7911
123.282-0.85290.5127.7374-2.64197.3389-0.23090.4280.2231-0.17530.3141-0.0425-0.2902-0.2227-0.09570.2527-0.04220.11910.4556-0.01390.4026-15.046-32.56343.2442
135.6669-2.24952.26270.8966-0.68443.92230.28480.6610.204-0.1818-0.3313-0.25630.14260.67640.06540.4051-0.04860.16110.42490.01280.3897-13.7684-38.058946.5218
149.595-2.7431-5.94742.4482.58865.6396-0.0228-0.64790.28320.11680.2043-0.24270.14830.3875-0.1880.2858-0.0203-0.07480.32320.02710.3214-15.3685-30.157767.3545
152.81090.83470.62140.76940.08651.41750.0330.03750.0134-0.0780.0343-0.0573-0.197-0.0236-0.07440.3013-0.00980.08140.3111-0.01420.333-21.9549-24.412153.5521
162.57650.79780.44591.3251.5563.82910.00280.20910.1472-0.1814-0.03280.1424-0.4876-0.390.03160.36480.03170.03160.33760.02590.378-29.9266-21.741948.9229
171.7202-2.06180.72087.5966-1.40910.5724-0.2416-0.0447-0.2929-0.10530.37720.440.0122-0.1079-0.14340.3136-0.05980.11570.3958-0.01760.2716-18.9503-26.632353.8443
185.69760.3187-0.94188.33915.93534.619-0.3771.14210.5329-1.0288-0.07250.6862-0.14170.25850.4170.3676-0.0277-0.02350.49510.15920.4469-19.81-19.967740.0608
193.4766-2.7137-2.20727.16624.0635.83450.1202-0.18030.518-0.58080.1701-0.5184-0.44350.1645-0.34090.36440.0390.10340.41920.00410.3601-35.4523-20.455618.4824
207.3159-1.0281-5.91947.37890.79828.2981-0.20280.2327-0.43080.0239-0.09330.20840.4376-0.18790.2790.3310.01640.01270.2925-0.030.3289-27.5273-43.16944.4045
211.2117-0.18250.24971.4652-0.28142.94630.05680.19640.2596-0.1898-0.08410.0830.14540.16270.01420.3061-0.02570.08520.4714-0.03030.3966-24.2599-26.5728-6.3646
225.9376-1.7809-1.34626.52281.76034.4452-0.16120.2487-0.47050.04-0.05670.78260.4419-0.48370.22510.3002-0.0923-0.01160.4344-0.03970.3228-40.2967-35.08211.4686
232.24120.408-1.24451.42290.99311.58620.19550.06810.25350.0366-0.0381-0.31140.17730.0077-0.13880.34620.01340.02540.3598-0.05470.3634-28.1309-25.59036.4173
244.59193.15812.00037.26791.59773.0153-0.0309-0.39360.33950.30360.0857-0.2387-0.09930.5271-0.02030.34370.04050.0020.5036-0.08180.3186-23.0522-21.076714.274
256.2482.479-2.14661.1979-1.42139.21050.71490.67940.80680.3692-0.0164-0.0655-0.1279-0.5736-0.69380.4065-0.03670.020.3563-0.11270.4421-30.3101-16.340410.6885
269.1443-2.98885.89544.7911-3.68176.0846-0.2596-0.86660.149-0.34420.2456-0.13770.1633-0.84360.03130.31160.03390.1280.3488-0.02350.3056-29.0526-26.36182.827
278.09380.2843.53145.9641-2.43462.7166-0.5233-0.18231.66931.320.1510.1821-0.53390.37190.34220.63260.06010.12660.5988-0.0890.6546-17.5605-15.37284.1056
282.0852-0.3767-1.37831.6395-0.57982.1436-0.0024-0.2566-0.1089-0.2109-0.0919-0.28570.20750.48530.07610.2708-0.0949-0.01370.43990.0340.5181-22.0026-67.230631.6721
295.2714.0884-0.98756.5764-2.52141.07280.05430.0570.49740.20620.06240.2929-0.1688-0.133-0.13320.32450.02250.00890.25950.00710.3287-38.0161-57.064324.8114
306.08440.96851.20313.1287-0.87557.82390.05470.1562-0.1199-0.36440.1905-0.18640.21370.2494-0.25030.31680.01550.05430.2547-0.02790.31-27.9409-73.693920.431
312.71830.1481-0.40991.9459-0.60721.7540.0107-0.18890.16480.1626-0.1083-0.3297-0.30160.19340.08760.3789-0.0546-0.01030.34250.05150.4233-29.0513-62.640637.5891
323.11762.5854-0.42174.40360.11541.8815-0.08520.2361-0.2577-0.2389-0.0202-0.3747-0.32730.02260.07210.30510.00250.0390.27740.05630.4529-32.0303-63.595829.6977
338.6196.42030.68596.8771-0.06055.1987-0.6614-0.1673-0.59980.87840.7485-0.6306-0.4490.0276-0.12020.65050.03630.0620.33780.09710.5799-34.4754-51.78637.1686
345.5833-1.77244.52667.3794-0.154.0984-0.13841.15840.23890.51980.2470.4099-0.52540.1649-0.1610.6175-0.03690.07780.36380.08730.3521-3.7891-40.048262.318
356.2659-5.41661.2745.48410.6213.86060.7207-0.7331-0.02520.14620.19891.0346-0.9275-0.3036-0.86530.8155-0.03520.38560.57630.08110.653-20.4406-56.849782.4973
367.20473.0295-2.32355.69-1.26339.52270.1878-0.83640.03590.4020.020.21571.26430.2035-0.22770.5629-0.0395-0.06660.33790.08180.6061-12.75-68.587176.2048
375.7349-4.0372-1.86188.32321.55124.09530.631-0.1815-0.1879-0.8886-0.18460.74820.1244-0.1438-0.37960.6489-0.0079-0.03310.42030.13680.5876-14.7436-66.840766.121
384.4977-0.79671.03936.7246-2.98285.47710.0539-0.3462-0.21510.36280.26651.0279-0.2645-0.5216-0.31130.4075-0.02610.01630.30850.01610.4613-16.2244-54.871569.9928
397.00870.2841-3.77795.2789-4.15226.05550.40820.05430.03230.47-0.19750.3959-0.228-0.2304-0.2420.46590.0126-0.02320.22460.02340.4169-10.9136-53.672767.3126
409.3241-3.23262.82832.0291-3.04118.0428-0.1630.45580.1789-0.1049-0.0126-1.57330.24781.12860.2960.4976-0.0572-0.02820.68410.08960.6467.7171-54.305869.796
412.0695-0.93722.38074.2664-1.85862.9329-0.0278-0.40510.76541.085-0.14320.4335-0.845-0.18850.12470.6489-0.06220.0730.2865-0.04740.4133-10.2901-45.976772.0762
429.68620.10751.39697.446-3.82134.89580.29510.0092-0.433-0.805-0.3731-0.64070.8475-0.07860.1270.4997-0.04830.07120.348-0.02060.3144-3.716-50.756762.5583
436.26280.33631.39613.6775-0.58831.73-0.0584-0.0628-0.2382-0.33080.28120.26530.0010.4903-0.16890.58750.0056-0.05560.32720.09410.3192-11.7869-59.057168.2034
446.54580.84163.98196.90881.8778.192-0.4003-0.3273-0.1218-0.3885-0.0898-1.0838-0.37530.01550.40740.59160.0646-0.00390.55120.10470.71661.8447-63.05170.8593
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 79 through 96 )A79 - 96
2X-RAY DIFFRACTION2chain 'A' and (resid 97 through 115 )A97 - 115
3X-RAY DIFFRACTION3chain 'A' and (resid 116 through 159 )A116 - 159
4X-RAY DIFFRACTION4chain 'A' and (resid 160 through 188 )A160 - 188
5X-RAY DIFFRACTION5chain 'A' and (resid 189 through 256 )A189 - 256
6X-RAY DIFFRACTION6chain 'A' and (resid 257 through 269 )A257 - 269
7X-RAY DIFFRACTION7chain 'A' and (resid 270 through 292 )A270 - 292
8X-RAY DIFFRACTION8chain 'B' and (resid 1 through 8 )B1 - 8
9X-RAY DIFFRACTION9chain 'C' and (resid 75 through 93 )C75 - 93
10X-RAY DIFFRACTION10chain 'C' and (resid 94 through 115 )C94 - 115
11X-RAY DIFFRACTION11chain 'C' and (resid 116 through 135 )C116 - 135
12X-RAY DIFFRACTION12chain 'C' and (resid 136 through 149 )C136 - 149
13X-RAY DIFFRACTION13chain 'C' and (resid 150 through 159 )C150 - 159
14X-RAY DIFFRACTION14chain 'C' and (resid 160 through 188 )C160 - 188
15X-RAY DIFFRACTION15chain 'C' and (resid 189 through 228 )C189 - 228
16X-RAY DIFFRACTION16chain 'C' and (resid 229 through 269 )C229 - 269
17X-RAY DIFFRACTION17chain 'C' and (resid 270 through 292 )C270 - 292
18X-RAY DIFFRACTION18chain 'D' and (resid 1 through 5 )D1 - 5
19X-RAY DIFFRACTION19chain 'E' and (resid 77 through 96 )E77 - 96
20X-RAY DIFFRACTION20chain 'E' and (resid 97 through 115 )E97 - 115
21X-RAY DIFFRACTION21chain 'E' and (resid 116 through 159 )E116 - 159
22X-RAY DIFFRACTION22chain 'E' and (resid 160 through 188 )E160 - 188
23X-RAY DIFFRACTION23chain 'E' and (resid 189 through 228 )E189 - 228
24X-RAY DIFFRACTION24chain 'E' and (resid 229 through 256 )E229 - 256
25X-RAY DIFFRACTION25chain 'E' and (resid 257 through 269 )E257 - 269
26X-RAY DIFFRACTION26chain 'E' and (resid 270 through 292 )E270 - 292
27X-RAY DIFFRACTION27chain 'F' and (resid 1 through 8 )F1 - 8
28X-RAY DIFFRACTION28chain 'G' and (resid 75 through 115 )G75 - 115
29X-RAY DIFFRACTION29chain 'G' and (resid 116 through 159 )G116 - 159
30X-RAY DIFFRACTION30chain 'G' and (resid 160 through 188 )G160 - 188
31X-RAY DIFFRACTION31chain 'G' and (resid 189 through 269 )G189 - 269
32X-RAY DIFFRACTION32chain 'G' and (resid 270 through 292 )G270 - 292
33X-RAY DIFFRACTION33chain 'H' and (resid 2 through 5 )H2 - 5
34X-RAY DIFFRACTION34chain 'I' and (resid 75 through 93 )I75 - 93
35X-RAY DIFFRACTION35chain 'I' and (resid 94 through 115 )I94 - 115
36X-RAY DIFFRACTION36chain 'I' and (resid 116 through 135 )I116 - 135
37X-RAY DIFFRACTION37chain 'I' and (resid 136 through 171 )I136 - 171
38X-RAY DIFFRACTION38chain 'I' and (resid 172 through 205 )I172 - 205
39X-RAY DIFFRACTION39chain 'I' and (resid 206 through 228 )I206 - 228
40X-RAY DIFFRACTION40chain 'I' and (resid 229 through 243 )I229 - 243
41X-RAY DIFFRACTION41chain 'I' and (resid 244 through 256 )I244 - 256
42X-RAY DIFFRACTION42chain 'I' and (resid 257 through 269 )I257 - 269
43X-RAY DIFFRACTION43chain 'I' and (resid 270 through 292 )I270 - 292
44X-RAY DIFFRACTION44chain 'J' and (resid 2 through 5 )J2 - 5

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