+Open data
-Basic information
Entry | Database: PDB / ID: 7wi6 | ||||||
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Title | Cryo-EM structure of LY341495/NAM-bound mGlu3 | ||||||
Components | Metabotropic glutamate receptor 3 | ||||||
Keywords | MEMBRANE PROTEIN / Glutamate / G protein-coupled receptor / Cryo-EM / Selective ligand | ||||||
Function / homology | Function and homology information group II metabotropic glutamate receptor activity / astrocyte projection / negative regulation of adenylate cyclase activity / G protein-coupled glutamate receptor signaling pathway / Class C/3 (Metabotropic glutamate/pheromone receptors) / glutamate receptor activity / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / G protein-coupled receptor activity ...group II metabotropic glutamate receptor activity / astrocyte projection / negative regulation of adenylate cyclase activity / G protein-coupled glutamate receptor signaling pathway / Class C/3 (Metabotropic glutamate/pheromone receptors) / glutamate receptor activity / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / G protein-coupled receptor activity / presynaptic membrane / gene expression / G alpha (i) signalling events / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / dendritic spine / postsynaptic density / axon / glutamatergic synapse / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.71 Å | ||||||
Authors | Fang, W. / Yang, F. / Xu, C.J. / Ling, S.L. / Lin, L. / Zhou, Y.X. / Sun, W.J. / Wang, X.M. / Liu, P. / Rondard, P. ...Fang, W. / Yang, F. / Xu, C.J. / Ling, S.L. / Lin, L. / Zhou, Y.X. / Sun, W.J. / Wang, X.M. / Liu, P. / Rondard, P. / Pan, S. / Pin, J.P. / Tian, C.L. / Liu, J.F. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell Res / Year: 2022 Title: Structural basis of the activation of metabotropic glutamate receptor 3. Authors: Wei Fang / Fan Yang / Chanjuan Xu / Shenglong Ling / Li Lin / Yingxin Zhou / Wenjing Sun / Xiaomei Wang / Peng Liu / Philippe Rondard / Pan Shi / Jean-Philippe Pin / Changlin Tian / Jianfeng Liu / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7wi6.cif.gz | 251.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7wi6.ent.gz | 185.5 KB | Display | PDB format |
PDBx/mmJSON format | 7wi6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/7wi6 ftp://data.pdbj.org/pub/pdb/validation_reports/wi/7wi6 | HTTPS FTP |
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-Related structure data
Related structure data | 32526MC 7wi8C 7wihC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 100021.695 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GRM3, GPRC1C, MGLUR3 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q14832 #2: Chemical | #3: Sugar | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Cryo-EM structure of mGlu3 bound with LY2794193 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 10 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 57.6 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software |
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EM software | Name: cryoSPARC / Category: CTF correction | ||||||||||||||||||||||||
CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.71 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 133783 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Stereochemistry target values: CDL v1.2 | ||||||||||||||||||||||||
Refine LS restraints |
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