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Open data
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Basic information
Entry | Database: PDB / ID: 7w0z | ||||||
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Title | Glycosyltranferase UGT74AN2 | ||||||
![]() | Glycosyltransferase | ||||||
![]() | TRANSFERASE / Complex with resibufagenin | ||||||
Function / homology | ![]() quercetin 7-O-glucosyltransferase activity / quercetin 3-O-glucosyltransferase activity / Transferases; Glycosyltransferases; Hexosyltransferases / nucleotide binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wei, H. / Feng, L. | ||||||
Funding support | 1items
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![]() | ![]() Title: Functional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated the Engineering Enhancement of Sugar Donor Promiscuity Authors: Huang, W. / He, Y. / Jiang, R. / Deng, Z. / Long, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 183.5 KB | Display | ![]() |
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PDB format | ![]() | 141.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7w09C ![]() 7w0kC ![]() 7w10C ![]() 7w11C ![]() 7w1bC ![]() 7w1hC ![]() 6l8zS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53952.184 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-UDP / |
#3: Chemical | ChemComp-6JI / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.14 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop / Details: PEG 3350, 0.1M sodium chloride, 0.1M MES (pH 6.5) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 26, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→48.69 Å / Num. obs: 38306 / % possible obs: 99.9 % / Redundancy: 17.7 % / Biso Wilson estimate: 21.91 Å2 / CC1/2: 0.956 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 1.9→1.94 Å / Num. unique obs: 2415 / CC1/2: 0.733 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6L8Z Resolution: 2.1→42.24 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 22.84 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 86.43 Å2 / Biso mean: 22.728 Å2 / Biso min: 9.28 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.1→42.24 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: 39.3571 Å / Origin y: 27.4822 Å / Origin z: 42.3791 Å
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Refinement TLS group |
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