Capsid vertex protein / Major capsid protein, Myoviridae / Major capsid protein Gp23 / Capsid protein, T4-like bacteriophage-like / T=13 icosahedral viral capsid / viral capsid / Major capsid protein / Capsid vertex protein
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI081726
米国
National Science Foundation (NSF, United States)
MCB-0923873
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2022 タイトル: Structures of a large prolate virus capsid in unexpanded and expanded states generate insights into the icosahedral virus assembly. 著者: Qianglin Fang / Wei-Chun Tang / Andrei Fokine / Marthandan Mahalingam / Qianqian Shao / Michael G Rossmann / Venigalla B Rao / 要旨: Many icosahedral viruses assemble proteinaceous precursors called proheads or procapsids. Proheads are metastable structures that undergo a profound structural transition known as expansion that ...Many icosahedral viruses assemble proteinaceous precursors called proheads or procapsids. Proheads are metastable structures that undergo a profound structural transition known as expansion that transforms an immature unexpanded head into a mature genome-packaging head. Bacteriophage T4 is a model virus, well studied genetically and biochemically, but its structure determination has been challenging because of its large size and unusually prolate-shaped, ∼1,200-Å-long and ∼860-Å-wide capsid. Here, we report the cryogenic electron microscopy (cryo-EM) structures of T4 capsid in both of its major conformational states: unexpanded at a resolution of 5.1 Å and expanded at a resolution of 3.4 Å. These are among the largest structures deposited in Protein Data Bank to date and provide insights into virus assembly, head length determination, and shell expansion. First, the structures illustrate major domain movements and ∼70% additional gain in inner capsid volume, an essential transformation to contain the entire viral genome. Second, intricate intracapsomer interactions involving a unique insertion domain dramatically change, allowing the capsid subunits to rotate and twist while the capsomers remain fastened at quasi-threefold axes. Third, high-affinity binding sites emerge for a capsid decoration protein that clamps adjacent capsomers, imparting extraordinary structural stability. Fourth, subtle conformational changes at capsomers' periphery modulate intercapsomer angles between capsomer planes that control capsid length. Finally, conformational changes were observed at the symmetry-mismatched portal vertex, which might be involved in triggering head expansion. These analyses illustrate how small changes in local capsid subunit interactions lead to profound shifts in viral capsid morphology, stability, and volume.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (1)
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aa: Major capsid protein ab: Major capsid protein ac: Major capsid protein ad: Major capsid protein ae: Major capsid protein af: Major capsid protein ag: Major capsid protein ah: Major capsid protein ai: Major capsid protein aj: Major capsid protein ak: Major capsid protein al: Major capsid protein am: Major capsid protein an: Major capsid protein ao: Major capsid protein ap: Major capsid protein aq: Major capsid protein ar: Major capsid protein as: Major capsid protein at: Major capsid protein au: Major capsid protein av: Major capsid protein aw: Major capsid protein ax: Major capsid protein ay: Major capsid protein az: Major capsid protein ba: Major capsid protein bb: Major capsid protein bc: Major capsid protein bd: Major capsid protein be: Major capsid protein bf: Major capsid protein bg: Major capsid protein bh: Major capsid protein bi: Major capsid protein bj: Major capsid protein bk: Major capsid protein bl: Major capsid protein bm: Major capsid protein bn: Major capsid protein bo: Major capsid protein bp: Major capsid protein bq: Major capsid protein br: Major capsid protein bs: Major capsid protein bt: Major capsid protein bu: Major capsid protein bv: Major capsid protein bw: Major capsid protein bx: Major capsid protein by: Major capsid protein bz: Major capsid protein ca: Major capsid protein cb: Major capsid protein cc: Major capsid protein cd: Major capsid protein ce: Major capsid protein cf: Major capsid protein cg: Major capsid protein ch: Major capsid protein ci: Major capsid protein cj: Major capsid protein ck: Major capsid protein cl: Major capsid protein cm: Major capsid protein cn: Major capsid protein co: Major capsid protein cp: Major capsid protein cq: Major capsid protein cr: Major capsid protein cs: Major capsid protein ct: Major capsid protein cu: Major capsid protein cv: Major capsid protein cw: Major capsid protein cx: Major capsid protein cy: Major capsid protein cz: Major capsid protein da: Major capsid protein db: Major capsid protein dc: Major capsid protein dd: Major capsid protein de: Major capsid protein df: Major capsid protein dg: Major capsid protein dh: Major capsid protein di: Major capsid protein dj: Major capsid protein dk: Major capsid protein dl: Major capsid protein dm: Major capsid protein dn: Major capsid protein do: Major capsid protein dp: Major capsid protein dq: Major capsid protein dr: Major capsid protein dy: Major capsid protein dz: Major capsid protein ea: Major capsid protein eb: Major capsid protein ec: Major capsid protein ed: Major capsid protein ee: Major capsid protein ef: Major capsid protein eg: Major capsid protein eh: Major capsid protein ei: Major capsid protein ej: Major capsid protein ek: Major capsid protein el: Major capsid protein em: Major capsid protein en: Major capsid protein eo: Major capsid protein ep: Major capsid protein eq: Major capsid protein er: Major capsid protein es: Major capsid protein et: Major capsid protein eu: Major capsid protein ev: Major capsid protein ew: Major capsid protein ex: Major capsid protein ey: Major capsid protein ez: Major capsid protein fa: Major capsid protein fb: Major capsid protein fc: Major capsid protein fd: Major capsid protein fe: Major capsid protein ff: Major capsid protein fg: Major capsid protein fh: Major capsid protein fi: Major capsid protein fj: Major capsid protein fk: Major capsid protein fl: Major capsid protein fm: Major capsid protein fn: Major capsid protein fo: Major capsid protein fp: Major capsid protein fq: Major capsid protein fr: Major capsid protein fs: Major capsid protein ft: Major capsid protein fu: Major capsid protein fv: Major capsid protein fw: Major capsid protein fx: Major capsid protein fy: Major capsid protein fz: Major capsid protein ga: Major capsid protein gb: Major capsid protein gc: Major capsid protein gd: Major capsid protein ge: Major capsid protein gf: Major capsid protein gg: Major capsid protein gh: Major capsid protein gi: Major capsid protein gj: Major capsid protein gk: Major capsid protein gl: Major capsid protein gm: Major capsid protein gn: Major capsid protein go: Major capsid protein gp: Major capsid protein gq: Major capsid protein gr: Major capsid protein gs: Major capsid protein gt: Major capsid protein gu: Major capsid protein gv: Major capsid protein gw: Major capsid protein gx: Major capsid protein gy: Major capsid protein gz: Major capsid protein ha: Major capsid protein hb: Major capsid protein hc: Major capsid protein hd: Major capsid protein he: Capsid vertex protein hf: Capsid vertex protein hg: Capsid vertex protein hh: Capsid vertex protein hi: Capsid vertex protein hj: Capsid vertex protein hk: Capsid vertex protein hl: Capsid vertex protein hm: Capsid vertex protein hn: Capsid vertex protein ho: Capsid vertex protein
aa: Major capsid protein ab: Major capsid protein ac: Major capsid protein ad: Major capsid protein ae: Major capsid protein af: Major capsid protein ag: Major capsid protein ah: Major capsid protein ai: Major capsid protein aj: Major capsid protein ak: Major capsid protein al: Major capsid protein am: Major capsid protein an: Major capsid protein ao: Major capsid protein ap: Major capsid protein aq: Major capsid protein ar: Major capsid protein as: Major capsid protein at: Major capsid protein au: Major capsid protein av: Major capsid protein aw: Major capsid protein ax: Major capsid protein ay: Major capsid protein az: Major capsid protein ba: Major capsid protein bb: Major capsid protein bc: Major capsid protein bd: Major capsid protein be: Major capsid protein bf: Major capsid protein bg: Major capsid protein bh: Major capsid protein bi: Major capsid protein bj: Major capsid protein bk: Major capsid protein bl: Major capsid protein bm: Major capsid protein bn: Major capsid protein bo: Major capsid protein bp: Major capsid protein bq: Major capsid protein br: Major capsid protein bs: Major capsid protein bt: Major capsid protein bu: Major capsid protein bv: Major capsid protein bw: Major capsid protein bx: Major capsid protein by: Major capsid protein bz: Major capsid protein ca: Major capsid protein cb: Major capsid protein cc: Major capsid protein cd: Major capsid protein ce: Major capsid protein cf: Major capsid protein cg: Major capsid protein ch: Major capsid protein ci: Major capsid protein cj: Major capsid protein ck: Major capsid protein cl: Major capsid protein cm: Major capsid protein cn: Major capsid protein co: Major capsid protein cp: Major capsid protein cq: Major capsid protein cr: Major capsid protein cs: Major capsid protein ct: Major capsid protein cu: Major capsid protein cv: Major capsid protein cw: Major capsid protein cx: Major capsid protein cy: Major capsid protein cz: Major capsid protein da: Major capsid protein db: Major capsid protein dc: Major capsid protein dd: Major capsid protein de: Major capsid protein df: Major capsid protein dg: Major capsid protein dh: Major capsid protein di: Major capsid protein dj: Major capsid protein dk: Major capsid protein dl: Major capsid protein dm: Major capsid protein dn: Major capsid protein do: Major capsid protein dp: Major capsid protein dq: Major capsid protein dr: Major capsid protein dy: Major capsid protein dz: Major capsid protein ea: Major capsid protein eb: Major capsid protein ec: Major capsid protein ed: Major capsid protein ee: Major capsid protein ef: Major capsid protein eg: Major capsid protein eh: Major capsid protein ei: Major capsid protein ej: Major capsid protein ek: Major capsid protein el: Major capsid protein em: Major capsid protein en: Major capsid protein eo: Major capsid protein ep: Major capsid protein eq: Major capsid protein er: Major capsid protein es: Major capsid protein et: Major capsid protein eu: Major capsid protein ev: Major capsid protein ew: Major capsid protein ex: Major capsid protein ey: Major capsid protein ez: Major capsid protein fa: Major capsid protein fb: Major capsid protein fc: Major capsid protein fd: Major capsid protein fe: Major capsid protein ff: Major capsid protein fg: Major capsid protein fh: Major capsid protein fi: Major capsid protein fj: Major capsid protein fk: Major capsid protein fl: Major capsid protein fm: Major capsid protein fn: Major capsid protein fo: Major capsid protein fp: Major capsid protein fq: Major capsid protein fr: Major capsid protein fs: Major capsid protein ft: Major capsid protein fu: Major capsid protein fv: Major capsid protein fw: Major capsid protein fx: Major capsid protein fy: Major capsid protein fz: Major capsid protein ga: Major capsid protein gb: Major capsid protein gc: Major capsid protein gd: Major capsid protein ge: Major capsid protein gf: Major capsid protein gg: Major capsid protein gh: Major capsid protein gi: Major capsid protein gj: Major capsid protein gk: Major capsid protein gl: Major capsid protein gm: Major capsid protein gn: Major capsid protein go: Major capsid protein gp: Major capsid protein gq: Major capsid protein gr: Major capsid protein gs: Major capsid protein gt: Major capsid protein gu: Major capsid protein gv: Major capsid protein gw: Major capsid protein gx: Major capsid protein gy: Major capsid protein gz: Major capsid protein ha: Major capsid protein hb: Major capsid protein hc: Major capsid protein hd: Major capsid protein he: Capsid vertex protein hf: Capsid vertex protein hg: Capsid vertex protein hh: Capsid vertex protein hi: Capsid vertex protein hj: Capsid vertex protein hk: Capsid vertex protein hl: Capsid vertex protein hm: Capsid vertex protein hn: Capsid vertex protein ho: Capsid vertex protein
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機能・相同性情報
Enterobacteria phage T4 (ファージ)
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米国, 2件 
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PDBj
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電子顕微鏡撮影
FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
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