National Natural Science Foundation of China (NSFC)
31870724
中国
National Natural Science Foundation of China (NSFC)
82030108
中国
National Natural Science Foundation of China (NSFC)
31872796
中国
National Natural Science Foundation of China (NSFC)
81571127
中国
National Natural Science Foundation of China (NSFC)
31730006
中国
National Natural Science Foundation of China (NSFC)
31600606
中国
引用
ジャーナル: Cell Res / 年: 2022 タイトル: Structural basis of ALMT1-mediated aluminum resistance in Arabidopsis. 著者: Jiangqin Wang / Xiafei Yu / Zhong Jie Ding / Xiaokang Zhang / Yanping Luo / Ximing Xu / Yuan Xie / Xiaoxiao Li / Tian Yuan / Shao Jian Zheng / Wei Yang / Jiangtao Guo / 要旨: The plant aluminum (Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance. Here we present cryo-electron ...The plant aluminum (Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance. Here we present cryo-electron microscopy (cryo-EM) structures of Arabidopsis thaliana ALMT1 (AtALMT1) in the apo, malate-bound, and Al-bound states at neutral and/or acidic pH at up to 3.0 Å resolution. The AtALMT1 dimer assembles an anion channel and each subunit contains six transmembrane helices (TMs) and six cytosolic α-helices. Two pairs of Arg residues are located in the center of the channel pore and contribute to malate recognition. Al binds at the extracellular side of AtALMT1 and induces conformational changes of the TM1-2 loop and the TM5-6 loop, resulting in the opening of the extracellular gate. These structures, along with electrophysiological measurements, molecular dynamic simulations, and mutagenesis study in Arabidopsis, elucidate the structural basis for Al-activated malate transport by ALMT1.
選択した粒子像数: 3586060 詳細: about 1,000 particles were manually picked from the micrographs for 2D classification initially. Class averages representing projections of AtALMT1 in different orientations were selected and ...詳細: about 1,000 particles were manually picked from the micrographs for 2D classification initially. Class averages representing projections of AtALMT1 in different orientations were selected and used as templates for automated particle picking
対称性
点対称性: C2 (2回回転対称)
3次元再構成
解像度: 3 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 216946 / アルゴリズム: FOURIER SPACE / クラス平均像の数: 1 / 対称性のタイプ: POINT