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- PDB-7v8g: Crystal structure of HOIP RING1 domain bound to IpaH1.4 LRR domain -
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Open data
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Basic information
Entry | Database: PDB / ID: 7v8g | ||||||||||||||||||
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Title | Crystal structure of HOIP RING1 domain bound to IpaH1.4 LRR domain | ||||||||||||||||||
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![]() | LIGASE / E3 ligase / ubiquitin / innate immune | ||||||||||||||||||
Function / homology | ![]() modulation of process of another organism / protein linear polyubiquitination / LUBAC complex / linear polyubiquitin binding / CD40 signaling pathway / RBR-type E3 ubiquitin transferase / positive regulation of xenophagy / CD40 receptor complex / negative regulation of necroptotic process / K48-linked polyubiquitin modification-dependent protein binding ...modulation of process of another organism / protein linear polyubiquitination / LUBAC complex / linear polyubiquitin binding / CD40 signaling pathway / RBR-type E3 ubiquitin transferase / positive regulation of xenophagy / CD40 receptor complex / negative regulation of necroptotic process / K48-linked polyubiquitin modification-dependent protein binding / TNFR1-induced proapoptotic signaling / positive regulation of protein targeting to mitochondrion / K63-linked polyubiquitin modification-dependent protein binding / TNFR1-induced NF-kappa-B signaling pathway / ubiquitin binding / Regulation of TNFR1 signaling / RING-type E3 ubiquitin transferase / cytoplasmic side of plasma membrane / protein polyubiquitination / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / positive regulation of canonical NF-kappaB signal transduction / host cell cytoplasm / protein ubiquitination / defense response to bacterium / ubiquitin protein ligase binding / zinc ion binding / extracellular region / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||||||||||||||
Biological species | Shigella flexneri serotype 5a![]() | ||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||
![]() | Liu, J. / Wang, Y. / Pan, L. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri. Authors: Liu, J. / Wang, Y. / Wang, D. / Wang, Y. / Xu, X. / Zhang, Y. / Li, Y. / Zhang, M. / Gong, X. / Tang, Y. / Shen, L. / Li, M. / Pan, L. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 165.8 KB | Display | ![]() |
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PDB format | ![]() | 103.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.3 KB | Display | ![]() |
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Full document | ![]() | 478.5 KB | Display | |
Data in XML | ![]() | 24.9 KB | Display | |
Data in CIF | ![]() | 33.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7v8eC ![]() 7v8fC ![]() 7v8hC ![]() 5edvS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
NCS oper:
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Components
#1: Protein | Mass: 26991.988 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: M90T / Gene: ipaH1.4, S0283, pWR501_0283 / Production host: ![]() ![]() References: UniProt: Q9AFJ5, RING-type E3 ubiquitin transferase #2: Protein | Mass: 11637.318 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q96EP0, RBR-type E3 ubiquitin transferase #3: Chemical | ChemComp-ZN / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.87 % |
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Crystal grow | Temperature: 289 K / Method: evaporation / pH: 8 Details: 2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(v/v) PEG 3,350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 11, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→72.78 Å / Num. obs: 18572 / % possible obs: 99.9 % / Redundancy: 12.8 % / Biso Wilson estimate: 53.84 Å2 / CC1/2: 0.995 / Rpim(I) all: 0.05 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.75→2.9 Å / Redundancy: 13.8 % / Mean I/σ(I) obs: 5.1 / Num. unique obs: 2667 / CC1/2: 0.963 / Rpim(I) all: 0.168 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5EDV Resolution: 2.75→60.36 Å / SU ML: 0.3307 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 30.2661 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 65.64 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.75→60.36 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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