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Yorodumi- PDB-7svf: Bile salt hydrolase A from Lactobacillus gasseri with taurine bound -
+Open data
-Basic information
Entry | Database: PDB / ID: 7svf | ||||||
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Title | Bile salt hydrolase A from Lactobacillus gasseri with taurine bound | ||||||
Components | Choloylglycine hydrolase | ||||||
Keywords | HYDROLASE / bile salt hydrolase | ||||||
Function / homology | : / 2-AMINOETHANESULFONIC ACID / : Function and homology information | ||||||
Biological species | Lactobacillus gasseri (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å | ||||||
Authors | Walker, M.E. / Redinbo, M.R. | ||||||
Funding support | United States, 1items
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Citation | Journal: Nat Microbiol / Year: 2023 Title: Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut. Authors: Foley, M.H. / Walker, M.E. / Stewart, A.K. / O'Flaherty, S. / Gentry, E.C. / Patel, S. / Beaty, V.V. / Allen, G. / Pan, M. / Simpson, J.B. / Perkins, C. / Vanhoy, M.E. / Dougherty, M.K. / ...Authors: Foley, M.H. / Walker, M.E. / Stewart, A.K. / O'Flaherty, S. / Gentry, E.C. / Patel, S. / Beaty, V.V. / Allen, G. / Pan, M. / Simpson, J.B. / Perkins, C. / Vanhoy, M.E. / Dougherty, M.K. / McGill, S.K. / Gulati, A.S. / Dorrestein, P.C. / Baker, E.S. / Redinbo, M.R. / Barrangou, R. / Theriot, C.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7svf.cif.gz | 274.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7svf.ent.gz | 216.2 KB | Display | PDB format |
PDBx/mmJSON format | 7svf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7svf_validation.pdf.gz | 1.8 MB | Display | wwPDB validaton report |
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Full document | 7svf_full_validation.pdf.gz | 1.8 MB | Display | |
Data in XML | 7svf_validation.xml.gz | 54.6 KB | Display | |
Data in CIF | 7svf_validation.cif.gz | 79.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sv/7svf ftp://data.pdbj.org/pub/pdb/validation_reports/sv/7svf | HTTPS FTP |
-Related structure data
Related structure data | 7sveC 7svgC 7svhC 7sviC 7svjC 7svkC 2hezS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35860.262 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lactobacillus gasseri (bacteria) / Gene: bsh, J3E66_000057 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8A6B131, choloylglycine hydrolase #2: Chemical | ChemComp-TAU / #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57.21 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2M Potassium Sulfate, 20% (w/v) PEG 3350. Crystals formed in a 1:2 protein (9.55 mg/mL) to mother liquor ratio. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Aug 11, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→45 Å / Num. obs: 100601 / % possible obs: 99.4 % / Redundancy: 1.9 % / CC1/2: 0.994 / CC star: 0.998 / Rmerge(I) obs: 0.067 / Rrim(I) all: 0.096 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.05→2.12 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.296 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 9994 / CC1/2: 0.81 / CC star: 0.946 / Rpim(I) all: 0.2958 / Rrim(I) all: 0.418 / % possible all: 99.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2HEZ Resolution: 2.05→45 Å / Cross valid method: FREE R-VALUE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 24.73 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.05→45 Å
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Refine LS restraints |
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