- PDB-7spy: Get3 bound to ATP from G. intestinalis in the closed form -
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データを開く
IDまたはキーワード:
読み込み中...
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基本情報
登録情報
データベース: PDB / ID: 7spy
タイトル
Get3 bound to ATP from G. intestinalis in the closed form
要素
ATPase ASNA1 homolog
キーワード
CHAPERONE / Tail-anchored membrane protein targeting Deviant Walker A ATPase targeting factor
機能・相同性
機能・相同性情報
GET complex / 加水分解酵素; 酸無水物に作用 / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / ATP hydrolysis activity / ATP binding / metal ion binding 類似検索 - 分子機能
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM097572
引用
ジャーナル: Nat Struct Mol Biol / 年: 2022 タイトル: Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3. 著者: Michelle Y Fry / Vladimíra Najdrová / Ailiena O Maggiolo / Shyam M Saladi / Pavel Doležal / William M Clemons / 要旨: Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) ...Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
解像度: 2.23→43.39 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.939 / SU B: 5.298 / SU ML: 0.127 / 交差検証法: THROUGHOUT / ESU R: 0.203 / ESU R Free: 0.169 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.20886
1199
5.1 %
RANDOM
Rwork
0.17763
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-
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obs
0.17929
22102
97.42 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK