+Open data
-Basic information
Entry | Database: PDB / ID: 7sl9 | ||||||
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Title | CryoEM structure of SMCT1 | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / transporter / TRANSPORT PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | Function and homology information propionate transmembrane transporter activity / propanoate transmembrane transport / short-chain fatty acid transmembrane transport / passive transmembrane transporter activity / monocarboxylate:sodium symporter activity / Organic anion transporters / organic acid:sodium symporter activity / monocarboxylic acid transmembrane transporter activity / lactate transmembrane transporter activity / NAD biosynthesis via nicotinamide riboside salvage pathway ...propionate transmembrane transporter activity / propanoate transmembrane transport / short-chain fatty acid transmembrane transport / passive transmembrane transporter activity / monocarboxylate:sodium symporter activity / Organic anion transporters / organic acid:sodium symporter activity / monocarboxylic acid transmembrane transporter activity / lactate transmembrane transporter activity / NAD biosynthesis via nicotinamide riboside salvage pathway / Nicotinamide salvaging / symporter activity / sodium ion transport / monoatomic ion transport / membrane => GO:0016020 / apical plasma membrane / apoptotic process / extracellular exosome / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) synthetic construct (others) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å | ||||||
Authors | Qu, Q. / Han, L. / Panova, O. / Feng, L. / Skiniotis, G. | ||||||
Funding support | 1items
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Citation | Journal: Nature / Year: 2022 Title: Structure and mechanism of the SGLT family of glucose transporters. Authors: Lei Han / Qianhui Qu / Deniz Aydin / Ouliana Panova / Michael J Robertson / Yan Xu / Ron O Dror / Georgios Skiniotis / Liang Feng / Abstract: Glucose is a primary energy source in living cells. The discovery in 1960s that a sodium gradient powers the active uptake of glucose in the intestine heralded the concept of a secondary active ...Glucose is a primary energy source in living cells. The discovery in 1960s that a sodium gradient powers the active uptake of glucose in the intestine heralded the concept of a secondary active transporter that can catalyse the movement of a substrate against an electrochemical gradient by harnessing energy from another coupled substrate. Subsequently, coupled Na/glucose transport was found to be mediated by sodium-glucose cotransporters (SGLTs). SGLTs are responsible for active glucose and galactose absorption in the intestine and for glucose reabsorption in the kidney, and are targeted by multiple drugs to treat diabetes. Several members within the SGLT family transport key metabolites other than glucose. Here we report cryo-electron microscopy structures of the prototypic human SGLT1 and a related monocarboxylate transporter SMCT1 from the same family. The structures, together with molecular dynamics simulations and functional studies, define the architecture of SGLTs, uncover the mechanism of substrate binding and selectivity, and shed light on water permeability of SGLT1. These results provide insights into the multifaceted functions of SGLTs. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7sl9.cif.gz | 113.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7sl9.ent.gz | 90.2 KB | Display | PDB format |
PDBx/mmJSON format | 7sl9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sl/7sl9 ftp://data.pdbj.org/pub/pdb/validation_reports/sl/7sl9 | HTTPS FTP |
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-Related structure data
Related structure data | 25195MC 7sl8C 7slaC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 67636.219 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SLC5A8, AIT, SMCT, SMCT1 / Production host: Homo sapiens (human) / References: UniProt: Q8N695 |
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#2: Antibody | Mass: 14441.961 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli BL21(DE3) (bacteria) |
#3: Chemical | ChemComp-BUA / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: SMCT1 and nanobody complex / Type: COMPLEX / Entity ID: #1-#2 / Source: MULTIPLE SOURCES |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Image recording | Electron dose: 56 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 173859 / Symmetry type: POINT |