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Open data
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Basic information
Entry | Database: PDB / ID: 7sjw | ||||||
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Title | Myocilin OLF mutant L303I | ||||||
![]() | Myocilin, C-terminal fragment![]() | ||||||
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Function / homology | ![]() skeletal muscle hypertrophy / clustering of voltage-gated sodium channels / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Scelsi, H.S. / Barlow, B.M. / Lieberman, R.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Quantitative differentiation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability. Authors: Scelsi, H.F. / Hill, K.R. / Barlow, B.M. / Martin, M.D. / Lieberman, R.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 90.4 KB | Display | ![]() |
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PDB format | ![]() | 52.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7sibC ![]() 7sijC ![]() 7sjtC ![]() 7sjuC ![]() 7sjvC ![]() 7skdC ![]() 7skeC ![]() 7skfC ![]() 7skgC ![]() 7t8dC ![]() 6pkeS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 31196.980 Da / Num. of mol.: 1 / Mutation: L303I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-GOL / ![]() |
#3: Chemical | ChemComp-CA / |
#4: Chemical | ChemComp-NA / |
#5: Water | ChemComp-HOH / ![]() |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.17 % |
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop / Details: 10% PEG 8000, 0.05M magnesium chloride |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 20, 2021 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.38→35.31 Å / Num. obs: 50211 / % possible obs: 97.82 % / Redundancy: 5.9 % / Biso Wilson estimate: 9.11 Å2 / CC1/2: 0.994 / CC star: 0.998 / Rmerge(I) obs: 0.1199 / Rpim(I) all: 0.05204 / Rrim(I) all: 0.131 / Net I/σ(I): 25.3 |
Reflection shell | Resolution: 1.38→1.429 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.4708 / Mean I/σ(I) obs: 7.03 / Num. unique obs: 4765 / CC1/2: 0.911 / CC star: 0.976 / Rpim(I) all: 0.2108 / Rrim(I) all: 0.5176 / % possible all: 93.83 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 6pke Resolution: 1.38→35.31 Å / SU ML: 0.1146 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 17.9304 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.52 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.38→35.31 Å
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Refine LS restraints |
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LS refinement shell |
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