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Yorodumi- PDB-7rle: Crystal structure of PPAR gamma in complex with CREB-binding prot... -
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-Basic information
Entry | Database: PDB / ID: 7rle | |||||||||
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Title | Crystal structure of PPAR gamma in complex with CREB-binding protein and agonist GW1929 | |||||||||
Components |
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Keywords | DNA BINDING PROTEIN / Nuclear Receptor / Transcription Factor | |||||||||
Function / homology | Function and homology information peptide lactyltransferase (CoA-dependent) activity / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / regulation of smoothened signaling pathway / histone H3K18 acetyltransferase activity / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / N-terminal peptidyl-lysine acetylation / histone H3K27 acetyltransferase activity ...peptide lactyltransferase (CoA-dependent) activity / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / regulation of smoothened signaling pathway / histone H3K18 acetyltransferase activity / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / N-terminal peptidyl-lysine acetylation / histone H3K27 acetyltransferase activity / NFE2L2 regulates pentose phosphate pathway genes / NFE2L2 regulating MDR associated enzymes / MRF binding / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / prostaglandin receptor activity / regulation of cholesterol transporter activity / negative regulation of connective tissue replacement involved in inflammatory response wound healing / negative regulation of receptor signaling pathway via STAT / MECP2 regulates transcription factors / negative regulation of extracellular matrix assembly / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / Regulation of FOXO transcriptional activity by acetylation / negative regulation of vascular endothelial cell proliferation / RUNX3 regulates NOTCH signaling / negative regulation of cellular response to transforming growth factor beta stimulus / Regulation of gene expression by Hypoxia-inducible Factor / Nuclear events mediated by NFE2L2 / NOTCH4 Intracellular Domain Regulates Transcription / negative regulation of cardiac muscle hypertrophy in response to stress / Regulation of NFE2L2 gene expression / arachidonate binding / positive regulation of low-density lipoprotein receptor activity / negative regulation of transcription by RNA polymerase I / positive regulation of adiponectin secretion / NOTCH3 Intracellular Domain Regulates Transcription / lipoprotein transport / negative regulation of sequestering of triglyceride / NFE2L2 regulating anti-oxidant/detoxification enzymes / TRAF6 mediated IRF7 activation / NFE2L2 regulating tumorigenic genes / peptide-lysine-N-acetyltransferase activity / FOXO-mediated transcription of cell death genes / DNA binding domain binding / macrophage derived foam cell differentiation / embryonic digit morphogenesis / STAT family protein binding / positive regulation of vascular associated smooth muscle cell apoptotic process / positive regulation of fatty acid metabolic process / response to lipid / homeostatic process / protein acetylation / negative regulation of SMAD protein signal transduction / LBD domain binding / Notch-HLH transcription pathway / negative regulation of type II interferon-mediated signaling pathway / positive regulation of transforming growth factor beta receptor signaling pathway / negative regulation of cholesterol storage / Formation of paraxial mesoderm / E-box binding / alpha-actinin binding / acetyltransferase activity / lipid homeostasis / stimulatory C-type lectin receptor signaling pathway / Zygotic genome activation (ZGA) / negative regulation of vascular associated smooth muscle cell proliferation / R-SMAD binding / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / monocyte differentiation / negative regulation of macrophage derived foam cell differentiation / negative regulation of blood vessel endothelial cell migration / negative regulation of lipid storage / cellular response to low-density lipoprotein particle stimulus / white fat cell differentiation / negative regulation of BMP signaling pathway / histone acetyltransferase complex / positive regulation of double-strand break repair via homologous recombination / Transcriptional and post-translational regulation of MITF-M expression and activity / negative regulation of mitochondrial fission / positive regulation of cholesterol efflux / Attenuation phase / positive regulation of fat cell differentiation / negative regulation of osteoblast differentiation / retinoic acid receptor signaling pathway / cell fate commitment / cellular response to nutrient levels / BMP signaling pathway / long-chain fatty acid transport / canonical NF-kappaB signal transduction / nuclear retinoid X receptor binding / Transcriptional regulation of brown and beige adipocyte differentiation by EBF2 / regulation of cellular response to heat / histone acetyltransferase activity / histone acetyltransferase / cell maturation / negative regulation of signaling receptor activity / epithelial cell differentiation / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / positive regulation of adipose tissue development / RORA activates gene expression / peroxisome proliferator activated receptor signaling pathway Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | |||||||||
Authors | Nemetchek, M.D. / Sprang, S.R. / Hughes, T.S. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2022 Title: A structural mechanism of nuclear receptor biased agonism. Authors: Nemetchek, M.D. / Chrisman, I.M. / Rayl, M.L. / Voss, A.H. / Hughes, T.S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7rle.cif.gz | 269.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7rle.ent.gz | 194 KB | Display | PDB format |
PDBx/mmJSON format | 7rle.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rl/7rle ftp://data.pdbj.org/pub/pdb/validation_reports/rl/7rle | HTTPS FTP |
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-Related structure data
Related structure data | 6d94SC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31506.574 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PPARG, NR1C3 / Production host: Escherichia coli (E. coli) / References: UniProt: P37231 #2: Protein/peptide | Mass: 2425.699 Da / Num. of mol.: 2 / Fragment: Residues 57-80 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) References: UniProt: Q92793, histone acetyltransferase, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.9 % / Description: 100um x 50um pentagonal prisms |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 1M sodium citrate tribasic dihydrate, 0.1M sodium cacodylate, pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 9, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→49.71 Å / Num. obs: 22792 / % possible obs: 98.19 % / Redundancy: 11.6 % / Biso Wilson estimate: 57.55 Å2 / CC1/2: 0.997 / CC star: 0.999 / Rmerge(I) obs: 0.1343 / Rpim(I) all: 0.04009 / Rrim(I) all: 0.1404 / Net I/σ(I): 13.49 |
Reflection shell | Resolution: 2.5→2.589 Å / Redundancy: 6.6 % / Rmerge(I) obs: 0.5342 / Mean I/σ(I) obs: 2.06 / Num. unique obs: 2104 / CC1/2: 0.737 / CC star: 0.921 / Rpim(I) all: 0.2083 / Rrim(I) all: 0.5765 / % possible all: 93.02 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6D94 Resolution: 2.5→49.71 Å / SU ML: 0.3388 / Cross valid method: FREE R-VALUE / σ(F): 1.4 / Phase error: 29.0831 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 70.48 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→49.71 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -25.682983496 Å / Origin y: 15.0090468547 Å / Origin z: -29.2334192563 Å
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Refinement TLS group | Selection details: all |