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- PDB-7qez: Crystal structure of the SARS-CoV-2 RBD in complex with the ultra... -

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Basic information

Entry
Database: PDB / ID: 7qez
TitleCrystal structure of the SARS-CoV-2 RBD in complex with the ultrapotent antibody CV2.1169 and CR3022
Components
  • CR3022 heavy chain
  • CR3022 light chain
  • CV2.1169 IgA heavy chain
  • CV2.1169 light chain
  • Spike protein S1
KeywordsVIRAL PROTEIN / neutralization / antibody / coronavirus
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
Biological speciesSevere acute respiratory syndrome coronavirus 2
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.89 Å
AuthorsFernandez, I. / Rey, F.A.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: J Exp Med / Year: 2022
Title: Potent human broadly SARS-CoV-2-neutralizing IgA and IgG antibodies effective against Omicron BA.1 and BA.2.
Authors: Cyril Planchais / Ignacio Fernández / Timothée Bruel / Guilherme Dias de Melo / Matthieu Prot / Maxime Beretta / Pablo Guardado-Calvo / Jérémy Dufloo / Luis M Molinos-Albert / Marija ...Authors: Cyril Planchais / Ignacio Fernández / Timothée Bruel / Guilherme Dias de Melo / Matthieu Prot / Maxime Beretta / Pablo Guardado-Calvo / Jérémy Dufloo / Luis M Molinos-Albert / Marija Backovic / Jeanne Chiaravalli / Emilie Giraud / Benjamin Vesin / Laurine Conquet / Ludivine Grzelak / Delphine Planas / Isabelle Staropoli / Florence Guivel-Benhassine / Thierry Hieu / Mikaël Boullé / Minerva Cervantes-Gonzalez / Marie-Noëlle Ungeheuer / Pierre Charneau / Sylvie van der Werf / Fabrice Agou / / / Jordan D Dimitrov / Etienne Simon-Lorière / Hervé Bourhy / Xavier Montagutelli / Félix A Rey / Olivier Schwartz / Hugo Mouquet /
Abstract: Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute to long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. ...Memory B-cell and antibody responses to the SARS-CoV-2 spike protein contribute to long-term immune protection against severe COVID-19, which can also be prevented by antibody-based interventions. Here, wide SARS-CoV-2 immunoprofiling in Wuhan COVID-19 convalescents combining serological, cellular, and monoclonal antibody explorations revealed humoral immunity coordination. Detailed characterization of a hundred SARS-CoV-2 spike memory B-cell monoclonal antibodies uncovered diversity in their repertoire and antiviral functions. The latter were influenced by the targeted spike region with strong Fc-dependent effectors to the S2 subunit and potent neutralizers to the receptor-binding domain. Amongst those, Cv2.1169 and Cv2.3194 antibodies cross-neutralized SARS-CoV-2 variants of concern, including Omicron BA.1 and BA.2. Cv2.1169, isolated from a mucosa-derived IgA memory B cell demonstrated potency boost as IgA dimers and therapeutic efficacy as IgG antibodies in animal models. Structural data provided mechanistic clues to Cv2.1169 potency and breadth. Thus, potent broadly neutralizing IgA antibodies elicited in mucosal tissues can stem SARS-CoV-2 infection, and Cv2.1169 and Cv2.3194 are prime candidates for COVID-19 prevention and treatment.
History
DepositionDec 3, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 18, 2022Provider: repository / Type: Initial release
Revision 1.1Jun 28, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Feb 7, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Spike protein S1
E: CR3022 heavy chain
F: CR3022 light chain
H: CV2.1169 IgA heavy chain
L: CV2.1169 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)119,7967
Polymers119,4795
Non-polymers3172
Water181
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8820 Å2
ΔGint-62 kcal/mol
Surface area37670 Å2
MethodPISA
Unit cell
Length a, b, c (Å)97.751, 97.751, 164.474
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number76
Space group name H-MP41

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Components

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Protein , 2 types, 2 molecules AE

#1: Protein Spike protein S1


Mass: 23068.695 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Drosophila melanogaster (fruit fly) / References: UniProt: P0DTC2
#2: Protein CR3022 heavy chain


Mass: 24321.242 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Drosophila melanogaster (fruit fly)

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Antibody , 3 types, 3 molecules FHL

#3: Antibody CR3022 light chain


Mass: 24376.963 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Drosophila melanogaster (fruit fly)
#4: Antibody CV2.1169 IgA heavy chain


Mass: 24165.072 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#5: Antibody CV2.1169 light chain


Mass: 23547.096 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Sugars , 1 types, 1 molecules

#6: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 2 molecules

#7: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.29 Å3/Da / Density % sol: 62.6 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 15% PEG 8000, 0.5 M lithium sulfate

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Data collection

DiffractionMean temperature: 77 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.98011 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jun 1, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98011 Å / Relative weight: 1
ReflectionResolution: 2.88→48.88 Å / Num. obs: 34600 / % possible obs: 99.4 % / Redundancy: 14 % / CC1/2: 0.995 / Rmerge(I) obs: 0.261 / Rpim(I) all: 0.072 / Rrim(I) all: 0.271 / Net I/σ(I): 10.2 / Num. measured all: 485839
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.88-3.0213.93.5096105544020.2140.9713.6430.996
9.56-48.8813.10.062129349860.9960.0180.06436.899.2

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
Aimless0.7.4data scaling
PDB_EXTRACT3.27data extraction
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6M0J, 7K3Q, 6YLA

6m0j

7k3q

6yla


Resolution: 2.89→48.88 Å / SU ML: 0.49 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 31.2 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.281 1785 5.19 %
Rwork0.2386 32579 -
obs0.2409 34364 99.77 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 223.36 Å2 / Biso mean: 91.2269 Å2 / Biso min: 42.24 Å2
Refinement stepCycle: final / Resolution: 2.89→48.88 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6613 0 19 1 6633
Biso mean--114.93 51.49 -
Num. residues----848
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 13

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.89-2.970.39391380.37042447258597
2.97-3.060.33461170.334425142631100
3.06-3.160.35261460.314825192665100
3.16-3.270.39951430.308424712614100
3.27-3.40.32241040.287125402644100
3.4-3.560.31491310.255224942625100
3.56-3.740.28131370.239725312668100
3.74-3.980.2681290.235225022631100
3.98-4.280.25411630.207924872650100
4.28-4.720.21861480.188924842632100
4.72-5.40.25211580.196525052663100
5.4-6.80.25751270.234525292656100
6.8-48.880.31431440.249925562700100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.8368-1.39531.8636.5312-4.10777.66840.9132-0.1398-0.25810.1634-0.5153-0.34021.11510.7349-0.35460.7310.0076-0.26270.6286-0.07040.6984152.962872.077831.0035
28.58263.43921.07512.56392.10582.9888-0.2893-0.76390.23230.97470.0569-1.21280.11871.01580.11820.95990.0257-0.1060.7021-0.09510.8017152.11483.363138.0969
35.32660.86125.2322.52171.79387.19460.19790.1943-0.1166-0.00990.1645-0.07890.181-0.1343-0.31910.5597-0.040.05270.51010.00090.4874138.495377.863418.046
48.46360.9948-3.35638.8965-3.23645.79690.3809-0.1307-0.18720.88730.32990.3383-0.40530.4256-0.7030.5167-0.0385-0.02880.4657-0.20340.5311148.573179.382928.0207
56.7343-3.952-2.13688.7724-1.44484.8901-0.06120.38040.8319-1.40130.7783-1.1314-0.34540.2393-0.49790.6373-0.24260.07620.6438-0.09890.6511151.5831113.120939.6721
66.4652-0.01180.55157.1708-2.66171.06850.192-0.5207-0.1306-0.13940.10340.00090.3794-0.0292-0.27610.5905-0.0851-0.0830.5773-0.10040.3745147.2406102.939340.3388
72.83521.5269-2.29452.54461.20415.33210.71530.6504-0.1413-0.1953-0.75450.1695-0.0238-0.9924-0.04910.8343-0.15770.04190.93520.01050.6436139.9851100.50333.1256
89.0528-5.06521.256.7759-1.14262.22881.03190.5880.3731-1.4225-0.3681-0.2473-0.84690.0673-0.72431.0609-0.03720.05130.5818-0.020.3484147.0337106.654431.7338
96.9318-2.53821.87752.7749-3.00083.32450.3502-0.0506-0.10520.94930.19010.876-0.6513-0.5953-0.49210.7380.01440.13810.6463-0.02260.3657143.1473113.001741.1739
107.41044.75482.73255.84325.23014.9989-0.6544-0.2678-0.3567-0.88760.2084-0.91660.3099-0.15590.33130.8381-0.0179-0.00210.60950.02460.5971148.374596.933144.4972
114.1103-1.6323-1.22733.74375.31027.58510.7256-0.19221.06550.9762-0.628-0.8847-1.0565-0.10340.06430.848-0.24170.00120.62560.04790.8729147.7122126.530544.5977
124.9405-1.71213.20112.24891.20549.0196-0.05080.1563-0.5346-0.41871.0667-1.1363-1.17950.6244-1.07290.7124-0.10420.14150.4466-0.15751.1204150.8526133.58365.0616
132.1045-3.52177.31583.2706-3.19225.33750.0798-0.0689-0.8496-0.4540.7688-0.80.16540.4941-0.70840.76650.00990.07210.8932-0.18070.7837156.5346126.500357.7112
146.45451.1197-1.94823.4741.6696.9386-0.0352-0.21250.9034-0.04680.721-0.10250.14770.2857-0.50270.5187-0.05590.04720.3802-0.1880.7177149.1247125.536561.1875
157.3079-1.42270.47716.02613.17212.3023-0.5831-1.18011.8210.73180.2573-0.41770.54510.29260.15330.60910.02390.02530.9803-0.15820.8415155.973130.30771.6585
169.44773.48318.27175.74910.96828.19040.28440.24131.0588-0.0876-0.1578-0.3209-0.58021.6354-0.57250.6653-0.0580.31790.6173-0.08851.1308158.8038133.998160.1243
176.42056.28945.369.66466.96625.3342-0.5034-0.98750.7233-0.6528-0.50711.4209-1.2282-1.39051.28920.3702-0.02480.0330.8613-0.11360.8827132.7432100.202358.1878
1810.03295.56944.86284.31064.00233.7933-0.6703-0.4559-0.65520.75690.39070.4504-0.6655-0.0820.09260.87550.02830.07050.6519-0.02870.636138.244895.707166.6199
194.86511.148-0.41470.6584-0.09991.99220.56450.0068-0.4147-0.94710.08410.13832.65580.1331-0.28151.4578-0.55870.03641.102-0.14920.741135.08783.880547.1657
207.31314.5132-0.71033.8791.89745.4385-0.7233-0.06250.5712-1.13390.76430.5261-0.01710.4311-0.00930.5303-0.0211-0.01490.5233-0.03320.4841146.529699.71255.1288
213.2136-0.45080.49263.58915.19118.5211-0.5073-0.3425-0.15761.63330.53380.27261.03340.4218-0.08751.0158-0.063-0.10520.60690.14140.6003143.356989.274558.6544
221.16520.0556-0.52644.96611.93011.797-0.0206-0.1850.1919-0.04710.1502-0.28170.11810.0632-0.16040.5043-0.03570.0540.59870.00410.5521142.5146111.917362.3577
233.8621-2.112-0.74035.3628-2.09572.6816-0.0711-0.08960.462-0.38830.2880.19740.5862-0.4246-0.21660.5162-0.0942-0.09640.7049-0.09040.4166136.7606127.985868.8335
244.752-3.4124-1.4193.01171.36552.96060.2163-0.39141.1328-0.49970.205-1.6238-0.09950.0722-0.39730.6186-0.03860.07860.6206-0.18910.5918142.9827127.041967.0569
253.4431-2.8331-1.28215.33131.53441.2461-0.175-0.22590.5790.32960.20030.04220.2235-0.129-0.04460.54390.0072-0.05070.9304-0.33690.6318135.7344133.552275.7975
263.31152.162-1.80581.5506-0.87562.31230.33820.31041.9777-1.02010.2320.8380.2927-0.87460.26241.45050.12090.25490.39420.02551.4243123.9749101.5772-3.6951
279.63130.8484-3.16047.87670.02295.26640.1406-0.13860.90270.1795-0.4120.33280.14070.02470.23280.52160.0751-0.03480.56290.03320.6892122.697592.1364-2.3778
288.0451-1.62870.42859.6361-3.31937.36540.57980.14550.23320.0888-0.35570.2748-0.060.2623-0.13090.48-0.04820.02160.54870.07420.7108130.678588.9253-3.8168
297.69945.2282-3.11469.9607-5.73819.36050.3138-0.0355-1.01340.9605-1.7063-1.1538-0.99441.36321.6210.68550.1364-0.20640.80150.07931.2092121.829390.1216-3.7832
308.91345.004-6.30233.8089-5.52447.87330.8045-0.82881.0204-0.8688-1.62870.3166-0.2781.83470.96190.86690.1155-0.01620.761-0.01491.057119.853996.2738-6.1584
312.9533-4.11991.30225.7648-1.77422.0011-0.25150.26170.86330.34090.5168-0.91851.73172.0277-0.06171.42370.1845-0.59230.96090.14061.5242117.150377.0048-17.4134
321.7323-0.88590.5728.5775-0.8970.17960.4788-0.20033.0391.0484-1.93682.4294-0.94340.46971.04380.6973-0.0461-0.03141.9969-0.31041.723396.051382.6179-16.6751
334.2246-2.72491.55193.88631.80174.3048-0.52140.86810.5036-1.12380.63580.8364-0.4162-0.21560.24820.5197-0.02420.07881.11780.71621.802112.425772.792-14.4907
349.69746.44173.5515.96493.4395.7528-0.1857-1.31251.0448-0.6854-0.02530.2227-0.5025-0.82020.15830.52860.06240.04680.81880.08681.2165110.663683.7794-5.6188
355.5166-1.64885.60097.5252-2.43645.94280.7057-1.43561.10580.2365-1.21832.74610.9905-3.29060.02670.8158-0.09840.00931.5368-0.36461.5251104.55579.2604-0.0198
369.4974-5.6435-1.78663.5932.04196.65212.5782.9926-1.0292-2.9232-3.56861.5088-1.4823-2.62131.2881.12740.5131-0.20191.4639-0.13221.18106.78772.6625-10.213
375.6031.5861-4.74420.8185-1.26534.0107-1.2103-1.87661.2453-0.2289-0.03920.4682-1.4068-0.64160.61841.1250.63390.16661.4926-0.33993.042396.632387.1275-7.7939
383.7188-2.0942-3.86882.55552.69284.2878-1.10692.54680.7679-0.28140.07840.88671.6475-0.52151.23060.9759-0.0309-0.17191.1655-0.00351.0188112.519782.787-10.9138
397.46062.0171-2.58583.17821.17742.26850.9558-5.2132-1.27550.2673-0.54981.39870.60791.2082-0.59110.43050.13650.03241.19310.08510.7849124.144176.6268-7.5765
405.5198-2.66234.58741.572-1.67674.6549-0.8441-0.0685-0.9896-0.3518-0.15820.4135-2.2124-1.61570.7121.12950.2456-0.30430.37880.05861.4306119.0681.7716-12.3076
415.0488-6.4132-5.82528.06567.34626.67830.7115-4.0708-1.8092.69770.7009-0.9817-1.90142.2027-0.67281.1047-0.1869-0.18111.03060.57231.6852100.992888.4397-17.5455
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 333 through 368 )A333 - 368
2X-RAY DIFFRACTION2chain 'A' and (resid 369 through 393 )A369 - 393
3X-RAY DIFFRACTION3chain 'A' and (resid 394 through 494 )A394 - 494
4X-RAY DIFFRACTION4chain 'A' and (resid 495 through 528 )A495 - 528
5X-RAY DIFFRACTION5chain 'E' and (resid 1 through 17 )E1 - 17
6X-RAY DIFFRACTION6chain 'E' and (resid 18 through 52 )E18 - 52
7X-RAY DIFFRACTION7chain 'E' and (resid 53 through 64 )E53 - 64
8X-RAY DIFFRACTION8chain 'E' and (resid 65 through 83 )E65 - 83
9X-RAY DIFFRACTION9chain 'E' and (resid 84 through 99 )E84 - 99
10X-RAY DIFFRACTION10chain 'E' and (resid 100 through 112 )E100 - 112
11X-RAY DIFFRACTION11chain 'E' and (resid 113 through 125 )E113 - 125
12X-RAY DIFFRACTION12chain 'E' and (resid 126 through 151 )E126 - 151
13X-RAY DIFFRACTION13chain 'E' and (resid 152 through 163 )E152 - 163
14X-RAY DIFFRACTION14chain 'E' and (resid 164 through 181 )E164 - 181
15X-RAY DIFFRACTION15chain 'E' and (resid 182 through 200 )E182 - 200
16X-RAY DIFFRACTION16chain 'E' and (resid 201 through 221 )E201 - 221
17X-RAY DIFFRACTION17chain 'F' and (resid 1 through 13 )F1 - 13
18X-RAY DIFFRACTION18chain 'F' and (resid 14 through 25 )F14 - 25
19X-RAY DIFFRACTION19chain 'F' and (resid 26 through 38 )F26 - 38
20X-RAY DIFFRACTION20chain 'F' and (resid 39 through 54 )F39 - 54
21X-RAY DIFFRACTION21chain 'F' and (resid 55 through 81 )F55 - 81
22X-RAY DIFFRACTION22chain 'F' and (resid 82 through 134 )F82 - 134
23X-RAY DIFFRACTION23chain 'F' and (resid 135 through 169 )F135 - 169
24X-RAY DIFFRACTION24chain 'F' and (resid 170 through 193 )F170 - 193
25X-RAY DIFFRACTION25chain 'F' and (resid 194 through 218 )F194 - 218
26X-RAY DIFFRACTION26chain 'H' and (resid 1 through 16 )H1 - 16
27X-RAY DIFFRACTION27chain 'H' and (resid 17 through 44 )H17 - 44
28X-RAY DIFFRACTION28chain 'H' and (resid 45 through 84 )H45 - 84
29X-RAY DIFFRACTION29chain 'H' and (resid 85 through 108 )H85 - 108
30X-RAY DIFFRACTION30chain 'H' and (resid 109 through 123 )H109 - 123
31X-RAY DIFFRACTION31chain 'L' and (resid 1 through 7 )L1 - 7
32X-RAY DIFFRACTION32chain 'L' and (resid 8 through 18 )L8 - 18
33X-RAY DIFFRACTION33chain 'L' and (resid 19 through 30 )L19 - 30
34X-RAY DIFFRACTION34chain 'L' and (resid 31 through 49 )L31 - 49
35X-RAY DIFFRACTION35chain 'L' and (resid 50 through 63 )L50 - 63
36X-RAY DIFFRACTION36chain 'L' and (resid 64 through 76 )L64 - 76
37X-RAY DIFFRACTION37chain 'L' and (resid 77 through 85 )L77 - 85
38X-RAY DIFFRACTION38chain 'L' and (resid 86 through 91 )L86 - 91
39X-RAY DIFFRACTION39chain 'L' and (resid 92 through 96 )L92 - 96
40X-RAY DIFFRACTION40chain 'L' and (resid 97 through 101 )L97 - 101
41X-RAY DIFFRACTION41chain 'L' and (resid 102 through 108 )L102 - 108

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