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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7pc8 | ||||||
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タイトル | The PDZ domain of SNTG1 complexed with the phosphomimetic mutant PDZ-binding motif of RSK1 | ||||||
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![]() | PEPTIDE BINDING PROTEIN / PDZ / complex / crystallization chaperone | ||||||
機能・相同性 | ![]() syntrophin complex / regulation of translation in response to stress / positive regulation of low-density lipoprotein particle receptor binding / CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling / positive regulation of receptor-mediated endocytosis involved in cholesterol transport / AnxA2-p11 complex / membrane raft assembly / dystrophin-associated glycoprotein complex / positive regulation of vacuole organization / positive regulation of low-density lipoprotein particle clearance ...syntrophin complex / regulation of translation in response to stress / positive regulation of low-density lipoprotein particle receptor binding / CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling / positive regulation of receptor-mediated endocytosis involved in cholesterol transport / AnxA2-p11 complex / membrane raft assembly / dystrophin-associated glycoprotein complex / positive regulation of vacuole organization / positive regulation of low-density lipoprotein particle clearance / phospholipase A2 inhibitor activity / ribosomal protein S6 kinase activity / CREB phosphorylation / hepatocyte proliferation / positive regulation of hepatic stellate cell activation / positive regulation of vesicle fusion / negative regulation of low-density lipoprotein particle receptor catabolic process / positive regulation of plasma membrane repair / positive regulation of plasminogen activation / PCSK9-AnxA2 complex / myelin sheath adaxonal region / cell communication / cadherin binding involved in cell-cell adhesion / Schmidt-Lanterman incisure / vesicle budding from membrane / cornified envelope / Gastrin-CREB signalling pathway via PKC and MAPK / plasma membrane protein complex / calcium-dependent phospholipid binding / negative regulation of receptor internalization / RSK activation / collagen fibril organization / Dissolution of Fibrin Clot / S100 protein binding / negative regulation of TOR signaling / virion binding / positive regulation of low-density lipoprotein receptor activity / osteoclast development / epithelial cell apoptotic process / positive regulation of receptor recycling / phosphatidylserine binding / ERK/MAPK targets / Recycling pathway of L1 / positive regulation of exocytosis / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / basement membrane / regulation of neurogenesis / Smooth Muscle Contraction / fibrinolysis / cytoskeletal protein binding / phosphatidylinositol-4,5-bisphosphate binding / protein serine/threonine/tyrosine kinase activity / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / lipid droplet / cell-matrix adhesion / response to activity / positive regulation of cell differentiation / adherens junction / lung development / calcium channel activity / mRNA transcription by RNA polymerase II / serine-type endopeptidase inhibitor activity / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / sarcolemma / nuclear matrix / ruffle membrane / RNA polymerase II transcription regulator complex / calcium-dependent protein binding / azurophil granule lumen / melanosome / late endosome membrane / actin binding / midbody / Senescence-Associated Secretory Phenotype (SASP) / positive regulation of cell growth / chemical synaptic transmission / basolateral plasma membrane / angiogenesis / collagen-containing extracellular matrix / protease binding / vesicle / early endosome / cytoskeleton / non-specific serine/threonine protein kinase / intracellular signal transduction / endosome / cell cycle / lysosomal membrane / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / synapse / calcium ion binding / Neutrophil degranulation / negative regulation of apoptotic process / structural molecule activity / positive regulation of DNA-templated transcription / cell surface / magnesium ion binding / signal transduction 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Cousido-Siah, A. / Trave, G. / Gogl, G. | ||||||
資金援助 | 1件
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![]() | ![]() タイトル: A scalable strategy to solve structures of PDZ domains and their complexes. 著者: Cousido-Siah, A. / Carneiro, L. / Kostmann, C. / Ecsedi, P. / Nyitray, L. / Trave, G. / Gogl, G. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 350.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 286.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 433.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 437.2 KB | 表示 | |
XML形式データ | ![]() | 17.5 KB | 表示 | |
CIF形式データ | ![]() | 26.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7pc3C ![]() 7pc4C ![]() 7pc5C ![]() 7pc7C ![]() 7pc9C ![]() 7pcbC ![]() 7qqlC ![]() 7qqmC ![]() 7qqnC ![]() 2pdzS ![]() 5n7dS S: 精密化の開始モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 46770.379 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質・ペプチド | 分子量: 1215.465 Da / 分子数: 2 / 由来タイプ: 合成 詳細: N-terminal biotin-ttds (trioxatridecan-succinamic acid) label 由来: (合成) ![]() 参照: UniProt: Q15418, non-specific serine/threonine protein kinase #3: 化合物 | ChemComp-CA / #4: 化合物 | ChemComp-GOL / #5: 水 | ChemComp-HOH / | 研究の焦点であるリガンドがあるか | N | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.96 Å3/Da / 溶媒含有率: 68.97 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 0.15 M Lithium sulfate, 0.05 M Magnesium chloride hexahydrate ,0.1 M HEPES 7.8, 20 % v/v PEG Smear High |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: DECTRIS PILATUS 2M / 検出器: PIXEL / 日付: 2021年6月14日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 2.5→48.334 Å / Num. obs: 51681 / % possible obs: 99.5 % / 冗長度: 6.83 % / CC1/2: 0.995 / Rrim(I) all: 0.198 / Net I/σ(I): 9.56 |
反射 シェル | 解像度: 2.5→2.56 Å / Mean I/σ(I) obs: 1.26 / Num. unique obs: 3761 / CC1/2: 0.532 / Rrim(I) all: 1.66 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 5N7D, 2PDZ 解像度: 2.5→48.334 Å / SU ML: 0.36 / 交差検証法: THROUGHOUT / σ(F): 1.35 / 位相誤差: 25.01 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 1.1 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso max: 148.84 Å2 / Biso mean: 61.7162 Å2 / Biso min: 29.78 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: final / 解像度: 2.5→48.334 Å
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LS精密化 シェル | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION
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精密化 TLSグループ |
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