- PDB-7pbk: Vibriophage phiVC8 family A DNA polymerase (DpoZ), two conformati... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 7pbk
Title
Vibriophage phiVC8 family A DNA polymerase (DpoZ), two conformations: thumb-exo open and thumb-exo closed
Components
DNA polymerase I
Keywords
VIRAL PROTEIN / phiVC8 / PolA / DpoZ / thumb-exo open / thumb-exo closed
Function / homology
Function and homology information
viral DNA genome replication / 3'-5' exonuclease activity / DNA-templated DNA replication / DNA-directed DNA polymerase activity / DNA binding Similarity search - Function
3'-5' exonuclease / 3'-5' exonuclease domain / DNA polymerase A / DNA polymerase family A / DNA-directed DNA polymerase, family A, palm domain / DNA polymerase A domain / Ribonuclease H superfamily / Ribonuclease H-like superfamily / DNA/RNA polymerase superfamily Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 133 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.62 Å3/Da / Density % sol: 53 %
Crystal grow
Temperature: 277.15 K / Method: vapor diffusion, hanging drop Details: 300 mM ammonium citrate; 12% PEG 3350. Seeded from crystals grown at 291.15 K in 1 mM hexamine cobalt; 25% v/v isopropanol (100%); 100 mM HEPES pH 7
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
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