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- PDB-7ozq: Crystal structure of archaeal L7Ae bound to eukaryotic kink-loop -

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Basic information

Entry
Database: PDB / ID: 7ozq
TitleCrystal structure of archaeal L7Ae bound to eukaryotic kink-loop
Components
  • 50S ribosomal protein L7Ae
  • RNA
KeywordsRIBOSOMAL PROTEIN / ribosome
Function / homology
Function and homology information


ribonuclease P activity / tRNA 5'-leader removal / rRNA processing / cytosolic small ribosomal subunit / rRNA binding / structural constituent of ribosome / translation
Similarity search - Function
Ribosomal protein L7Ae, archaea / Ribosomal protein L7Ae conserved site / Ribosomal protein L7Ae signature. / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like
Similarity search - Domain/homology
ACETATE ION / RNA / RNA (> 10) / Large ribosomal subunit protein eL8
Similarity search - Component
Biological speciesPyrococcus furiosus (archaea)
Saccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.91 Å
AuthorsHoefler, S. / Lukat, P. / Carlomagno, T. / Blankenfeldt, W.
CitationJournal: Sci Rep / Year: 2021
Title: Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites.
Authors: Hofler, S. / Lukat, P. / Blankenfeldt, W. / Carlomagno, T.
History
DepositionJun 28, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 27, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: 50S ribosomal protein L7Ae
B: 50S ribosomal protein L7Ae
C: 50S ribosomal protein L7Ae
D: 50S ribosomal protein L7Ae
E: RNA
F: RNA
G: RNA
H: RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)97,30123
Polymers96,7138
Non-polymers58915
Water10,755597
1
A: 50S ribosomal protein L7Ae
H: RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)24,2734
Polymers24,1782
Non-polymers942
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1470 Å2
ΔGint-23 kcal/mol
Surface area11190 Å2
MethodPISA
2
B: 50S ribosomal protein L7Ae
E: RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)24,3766
Polymers24,1782
Non-polymers1984
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1540 Å2
ΔGint-35 kcal/mol
Surface area10950 Å2
MethodPISA
3
C: 50S ribosomal protein L7Ae
F: RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)24,3698
Polymers24,1782
Non-polymers1916
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1930 Å2
ΔGint-54 kcal/mol
Surface area10900 Å2
MethodPISA
4
D: 50S ribosomal protein L7Ae
G: RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)24,2835
Polymers24,1782
Non-polymers1053
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1650 Å2
ΔGint-23 kcal/mol
Surface area11030 Å2
MethodPISA
Unit cell
Length a, b, c (Å)109.391, 61.598, 138.611
Angle α, β, γ (deg.)90.000, 108.356, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Components on special symmetry positions
IDModelComponents
11E-102-

CA

21F-106-

CA

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Components

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Protein / RNA chain , 2 types, 8 molecules ABCDEFGH

#1: Protein
50S ribosomal protein L7Ae / Large ribosomal subunit protein eL8 / Ribosomal protein L8e


Mass: 13542.794 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (archaea)
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1 / Gene: rpl7ae, PF1367 / Plasmid: pETM11 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q8U160
#2: RNA chain
RNA


Mass: 10635.354 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast)

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Non-polymers , 5 types, 612 molecules

#3: Chemical
ChemComp-ACT / ACETATE ION


Mass: 59.044 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C2H3O2
#4: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Na
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Ca
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: Cl
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 597 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.29 Å3/Da / Density % sol: 46.32 %
Crystal growTemperature: 291 K / Method: vapor diffusion / pH: 4.6
Details: JCSG Core I, condition G12: 30% MPD, 0.02 M CaCl2, 0.1 M Na acetate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, DESY / Beamline: P11 / Wavelength: 1.0332 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 28, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0332 Å / Relative weight: 1
ReflectionResolution: 1.91→54.5 Å / Num. obs: 68501 / % possible obs: 100 % / Redundancy: 6.8 % / Biso Wilson estimate: 31.36 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.081 / Rpim(I) all: 0.034 / Rrim(I) all: 0.088 / Net I/σ(I): 12.4 / Num. measured all: 463836 / Scaling rejects: 1
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
1.91-2.016.60.6576567499230.8750.2750.7132.4100
6.03-54.56.80.0471555622720.9970.0190.05131.699.7

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation2.4 Å54.5 Å
Translation2.4 Å54.5 Å

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Processing

Software
NameVersionClassification
PHENIX1.18rc4_3812refinement
XDSdata reduction
Aimless0.7.4data scaling
PHASER2.8.3phasing
PDB_EXTRACT3.25data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4BW0
Resolution: 1.91→54.5 Å / SU ML: 0.2094 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 23.3179
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2185 3281 4.79 %
Rwork0.1728 65194 -
obs0.1749 68475 99.93 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 38.91 Å2
Refinement stepCycle: LAST / Resolution: 1.91→54.5 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3690 2728 30 597 7045
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0096886
X-RAY DIFFRACTIONf_angle_d0.91349947
X-RAY DIFFRACTIONf_chiral_restr0.05461251
X-RAY DIFFRACTIONf_plane_restr0.0075805
X-RAY DIFFRACTIONf_dihedral_angle_d18.31823024
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.91-1.940.29421230.23712812X-RAY DIFFRACTION99.93
1.94-1.970.29291520.23052848X-RAY DIFFRACTION100
1.97-20.27141590.22682733X-RAY DIFFRACTION100
2-2.030.28131370.21192857X-RAY DIFFRACTION99.87
2.03-2.070.2631530.20082814X-RAY DIFFRACTION99.9
2.07-2.110.23821430.18872812X-RAY DIFFRACTION99.97
2.11-2.150.3151430.19952806X-RAY DIFFRACTION99.76
2.15-2.20.22921630.18982796X-RAY DIFFRACTION100
2.2-2.250.26991300.1912813X-RAY DIFFRACTION99.97
2.25-2.310.22591430.18132831X-RAY DIFFRACTION99.97
2.31-2.370.27111560.17842821X-RAY DIFFRACTION100
2.37-2.440.24371280.18062848X-RAY DIFFRACTION99.97
2.44-2.520.25311330.18642842X-RAY DIFFRACTION100
2.52-2.610.26131330.1992849X-RAY DIFFRACTION100
2.61-2.710.24641420.19952804X-RAY DIFFRACTION99.93
2.71-2.830.27671390.19422851X-RAY DIFFRACTION100
2.83-2.980.21961330.18442864X-RAY DIFFRACTION99.97
2.98-3.170.22851490.1712798X-RAY DIFFRACTION99.9
3.17-3.420.19761280.162880X-RAY DIFFRACTION99.93
3.42-3.760.19451620.15142820X-RAY DIFFRACTION99.97
3.76-4.30.17711350.13942872X-RAY DIFFRACTION100
4.3-5.420.16551380.14312882X-RAY DIFFRACTION99.9
5.42-54.50.19881590.18062941X-RAY DIFFRACTION99.55
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.005116725843240.00452499029063-0.005545152010546.78802998167E-6-0.002261851972370.01025223717230.0355816309152-0.1235297097040.0276527941410.0997028298965-0.01333370890570.03077430475750.103120137830.192335507471-2.30211402974E-50.2822974313790.003047516075470.0171181957550.364168243647-0.02308122916760.298797605236-10.62261683014.7871750143816.1392585054
20.00178351919174-0.00174691972203-0.000324233793220.01753866387210.01255849536630.011641665010.03916668507410.05564826092460.115497515625-0.0149084903941-0.0321537755015-0.0325407385136-0.27372458045-0.0153745784792-2.05159717932E-50.2702194272250.009569829449390.03906489187880.198294099717-0.04263002274540.333359094202-27.569052644910.87627849719.90884312836
30.0105882513660.01894728317770.00800472757890.02289095120720.006457554185790.0005889665834240.07358958073350.034111818755-0.149371493675-0.1942722453-0.0539019813384-0.07502192862910.227033394128-0.125305499276-2.40303680088E-50.3914606871860.01802180497330.005793940956970.218401591761-0.04072304818080.346575901593-26.9848157916-6.23195194248.20517597938
40.1644204492520.0510262164643-0.1169425351180.0254248313707-0.0393856506310.08256533417670.0132857711578-0.131408013355-0.233861229047-0.135430203437-0.133949218168-0.1380272008490.1546997516090.187454461125-0.01483068126820.2831236412930.0357808346580.04454976922210.2145772116980.01763344604550.189755014778-19.963292756-2.655396229313.1944821258
50.05893684097480.0129994810068-0.002012879179840.0307054394609-0.02471264432680.0149101367015-0.05207118492040.0833072448939-0.221339672972-0.326744635944-0.0812892679114-0.002993003160110.1870780719180.17684292552-2.23571973112E-50.3568091481870.05204654994960.07207602820040.232164843403-0.0361480657640.276655053726-15.8270588326-1.641771739077.98876159689
60.0100869950335-0.01060956767270.0008253314396550.00559171962627-0.00061098859630.0110451924206-0.123703062277-0.132224991433-0.01589331799170.129151526933-0.0148366332337-0.06699545589440.09264270007770.0664724755838-0.0002880590120480.2445887953730.003874523901490.0513652437460.247017092077-0.03255604453090.250698258522-27.41701867385.2737119904819.2629031573
70.04617830173730.006008098395190.02507265768280.03547035370750.00204791251380.01006873862730.1088660455080.0878243299285-0.078900684348-0.296009388835-0.05571517503970.0841842809386-0.0586925496074-0.3895136665156.80559866069E-50.2511479061480.01126311847590.02216827516680.229500682784-0.01849527429290.231756952135-29.57163013790.9196649593411.8672857519
80.04110491933190.01552474455140.02264901443770.3309736335120.3064407245650.289420670673-0.07931326910630.2609975816960.0698010485654-0.449402337335-0.09472894469530.00352978340479-0.1708359651810.288107239527-0.07544144115340.3714930104910.01069457458250.09452403867050.2926375136320.1016884366670.369514171265-17.76687551949.790352611581.76293318686
90.0560472353105-0.02816568335040.01476010792450.0151756163864-0.005293415824390.003909948103740.1019769519080.114173182159-0.0896300187764-0.1663815522910.1270527979330.002349666518780.0696138560204-0.02047767453220.02066479614320.3207606290740.0747926620114-0.1401232709330.490100535426-0.01888760666980.376494584696-19.1336331574-10.853165342738.5678497997
100.0599065029543-0.0120145952165-0.04359833129750.0229570732910.03134639886440.04763367772560.002864204109360.06050829996150.1735791890930.1034097363010.01378757260370.0122264617463-0.2112423709090.06812050291671.74442259437E-50.378776984253-0.000471878636583-0.01347294142330.2669070093550.02860663427060.342739204907-9.30964954599-7.7759831158653.7994654727
110.143439925205-0.0773514780017-0.009103727049020.08671962407050.08638718748940.1238705738560.1737494728070.100881543358-0.435446042391-0.150663643036-0.1214437146520.2663956247450.365916583028-0.07458405843172.25409593676E-50.36819264821-0.0282420660919-0.02726049832420.360909873133-0.02903850174250.411570615363-16.0870415853-19.023412054547.7050255953
120.0528568139610.004868722964830.07127161142820.13054664810.1260073939360.1681763300950.05739429001310.0964256332592-0.1110067242840.0533169954919-0.06920949190990.142832851351-0.124203232006-0.1342761275812.55481143448E-60.2941676572490.0130435402504-0.0009304923500910.327760473722-0.006266076022050.328911521456-13.4455071897-9.6904823439152.1482385985
130.007346271712180.00189947114718-0.0004915710446260.004560980782340.004926333644470.001922773845030.178855930652-0.0810087658114-0.03607682101140.2145031614660.09696566660560.0566861958616-0.102146924825-0.06821608123585.31917031401E-50.381719636875-0.06064105174460.0659065066330.3838648076710.0393218533130.2849376898692.88733273237-14.573535726828.0677076587
140.00287499285441-0.00782319406138-0.008839578077550.01871333771470.02104077762650.01823397292720.1104245944060.101875395549-0.263084101393-0.01197383387670.0820837895479-0.02071242524540.2715983053990.118332812667-0.0001089477830210.3362232844350.02831849244020.03272905837990.236743046018-0.001984170572540.31263837242812.3180667203-19.412963555512.2069341213
150.007607377222070.00693905801644-0.004371056439920.00656293559251-0.002675329514970.01024803280230.05928965412020.06176182917740.0758315330777-0.157280061724-0.03300932817630.0384007636592-0.01360953165370.09343224410757.47776024194E-50.3349451160150.02289268079240.04906635639810.2653122424560.02658487024090.33465494437614.4808014231-6.865570759218.88076986585
160.1988620016630.169703645766-0.03966651214180.1821459698290.05162606312480.1114887703390.0995031893747-0.1883553466020.2038206959180.296461045399-0.02617824109830.172138840082-0.33530247804-0.05039663178720.004093345780210.2823168342710.01878684355430.04886703399340.255316911225-0.004872352990930.2524363765385.68934618054-5.4282731661818.2575947501
170.08933559167110.100027307694-0.04935812140190.1445478837090.03339928507230.2170245250920.0708391423162-0.0279312747659-0.158646430824-0.0118359654897-0.07104658057440.06373221614670.196045517341-0.06717521900232.592996416E-50.248727719989-0.01278117543250.006531949954240.276014800095-0.004424604532780.2755314806948.6768480441-15.031203134813.4249678004
180.00320747345914-0.001386861972620.00546170927725-0.00118189653301-0.001119629919650.00598945948962-0.07181046335640.208863218688-0.100483132382-0.01343427387520.1318453347930.01705424323440.05112190756580.362627088983-1.80413313113E-50.302356467467-0.0212725652613-0.002375307185930.483626827581-0.06589654879820.45105987939821.90961633281.5670055997350.8282069487
190.01373819719910.006905596086260.02184633540360.01735379744560.0105361771470.0294725416299-0.01454685407620.0364006635704-0.2669768346930.125343552664-0.01488647492040.1556953602280.237168521919-0.0920716245326-6.75469822767E-50.3701296382980.02221788728580.007447279660510.250121434523-0.0706112043670.420835761155.86004966665-5.1651597768256.1010780069
200.006120140192280.00314316185273-0.007673804405920.00884913267691-0.0006236386566630.00757650279559-0.0953166415608-0.008824678958090.02578825691220.0399748344021-0.0658693452470.06766378155580.00385568436636-0.111823645867-2.33273094731E-50.409361457276-0.00244405634336-0.03627328059540.267358949281-0.07549599489780.3867893183391.069768239397.5845349235957.5766595565
210.727472937336-0.147657921560.5378235403350.0437082469951-0.1294638789020.40753294729-0.2464713581480.3781960405670.6815509593060.120624737217-0.0577973926022-0.133910046679-0.1788549950550.217712827122-0.05240970972030.321162815962-0.0616523269171-0.0729013653040.2463728742530.03882031255420.43601029314611.526037591310.367008111653.7302189456
220.008340322645230.008707076972720.003074139420420.00802160070329-0.00183718961980.00653277251009-0.04365151604750.01517318583390.2062677745290.2464653863840.181745254935-0.0349530314132-0.06288566308110.107604621929-0.0001110991890920.397869019547-0.0797362890208-0.07179816531460.351066908302-0.04149347767770.51748844752218.356107721312.258771309558.7806023887
230.1271785776250.0606629111024-0.05208626306320.111636032526-0.08155499566210.0526531667543-0.05883752461620.218489713520.1613118367380.1660759313970.09262190384310.26508429025-0.01921534428340.0611058678027-4.38319783272E-50.298912227053-0.0104928442146-0.03740392238510.258841868822-0.01235575676670.2761957766617.772371750793.3116941561953.4448315561
240.0215844070276-0.01297415095230.02090459042730.02993796249320.01764122117660.04130711771560.0176743872073-0.2347313035810.01546382690990.5002035256890.02660770725260.03919678306950.2472146320420.341981503173-0.001278612437140.4393898234920.0235970175706-0.1187959993750.3507611225130.03045536252760.42590035983314.7210110171-3.4992822789264.4942140256
250.005191247634220.0111435051378-0.003513894308010.0100411185605-0.001102415048180.001672399764170.16643260118-0.020752499733-0.123025794688-0.1275846712640.07995453162240.3682370898860.02252697599470.09765902630527.2943530061E-50.645656638572-0.140670935521-0.1603145750730.4878066011110.1794338555531.16739893353-5.44327858304-33.475231388852.6648769432
260.2329298421490.1171203918540.145832471210.1366554686520.04580752184310.03391100857240.3580973266420.333696407492-0.162687208802-0.0737453366821-0.2275892774050.08156653161540.0971100338122-0.01208814296820.01464383715380.3157441434030.095510811001-0.01843147069620.318747317966-0.01548450225160.26090278321416.9695198146-22.704117280250.8051127168
27-0.000180386492573-0.000650015891309-0.001739281043180.00859602906479-0.001233454404810.00507073118801-0.02887997977460.0564539158489-0.08747412051640.0421500760906-0.0819829169717-0.06467168837010.0328502795405-0.001639333564290.0002827799289730.6832514254870.2644115824570.008452591358650.536011523765-0.1494197115480.6071244514515.06155740309-28.817669765947.104740083
280.0130197140602-0.01592756059970.001296543453290.00979530866857-7.72881467962E-5-0.0006170828821030.0962346516107-0.0101170251418-0.06272205126020.109372286972-0.1014580534930.211126137872-0.1457710651550.06377501281881.09574737778E-60.6871435849830.009378005512760.03589875219410.369256276160.02232740792010.92645476172116.15462275228.6902372501312.8513956604
290.006111356231480.03309382971180.03452793002780.1168086241850.1107009346970.08746854454240.133003845177-0.03353966187560.257080511225-0.0128309532694-0.153848157618-0.153002660792-0.4446894221420.100115717285-0.006505081745310.285754334811-0.1234295842280.03065314730930.470048355823-0.000386411532360.34140741182132.59637125630.20918520279716.2781739347
300.023646205798-0.00653288349708-0.05800820590030.00823216319163-0.01185784749930.05696361382010.384094487665-0.1923203799760.0313710349436-0.0423349881943-0.0171955441868-0.004534965413230.0478669739177-0.074040790089-1.88718204666E-50.320271573314-0.05428552545650.03843913784160.3426237678510.05130379207490.30006927223148.79610683380.83763380603510.0231546484
310.0242243380894-0.0266649434815-0.04253095201280.1452191090170.08286374676920.05079517941890.32311016198-0.4115862172530.009942592290860.0345444503102-0.210855580675-0.0276954373524-0.1524927321920.1411877283680.019714474890.257194812423-0.163312179037-0.006109283514320.4232098617920.02616982337560.26135909520430.3971801491-4.2785935931519.73590299
320.0139718383852-0.00255796375971-0.01125763207420.008168421935210.001750790223070.00451220731715-0.0469403479310.0544787497540.104423002897-0.0781224192639-0.0689135268696-0.0429808902082-0.1121889379870.272440131286-2.90907402981E-50.501858854884-0.0995838327462-0.2930819729070.4901249942250.07705630767460.7880107464091.3711308480722.408115776553.8335450304
330.1687480288570.03239062551740.1649442825690.05127738606930.05297791389540.162899585888-0.09281905341970.1452055479590.205433129906-0.1996439507990.1039885264480.238494478356-0.0766460534015-0.1301984827520.05002237922890.374193442692-0.0421228155554-0.7478478804840.4452664240720.3263499476070.950822686628-8.635032528613.09113832139.2696378571
340.003791126634910.00631335473864-0.02334454648860.00793449800187-0.0383139339350.0971758263455-0.1029816579150.2671155764380.1422461353630.1010699456870.117758116065-0.0587224258069-0.3171105214-0.40834700787-4.52159028468E-50.3780580795810.0798855939660.02488064122840.3418718992760.009847741764640.1980059385-24.510976500111.114960100735.0021584015
350.2733921561970.115078220069-0.123447193880.022692315915-0.05644944658460.0737847262646-0.3168313802420.7773601641890.5272613314720.24302330815-0.122312689502-0.208951565671-0.3542561747780.354601346548-1.080288015660.376579157333-0.0693476771081-0.2340376854220.4147837008090.2605232487260.386179063778-4.263355646079.647140471138.7737674443
360.00311392957131-0.005824492035390.006327826206270.009754861035860.001476368005670.002829402971860.0696218473363-0.152430835278-0.03784892146310.114299934101-0.0198380518791-0.11232405183-0.05843595436670.3422482218053.33406354369E-50.59257228188-0.1005489068210.131365813930.582446782768-0.1681116901880.732412591759-31.6404613769-17.308643811711.7150366479
370.01941935184890.0296087828227-0.02364685029630.08538284737340.02930685583350.0480095209877-0.1150417583860.0283508484133-0.33675038054-0.136661640425-0.0605504837239-0.171794474540.125961735304-0.04448980883920.0002063905544820.318462032324-0.07784419935820.05048356644880.3738643981590.0829449820130.323500048528-48.7713139602-5.05428409430.4927944331
380.06392462032760.05785159908040.04744829951960.08473946744290.1631040206570.43726501990.266411311111-0.131886971028-0.0224585499842-0.01546150823320.05971429366480.1499622288130.413311269008-0.251412388460.09343143985990.519037448348-0.181578190086-0.1041914779630.3293672139570.1128435659440.323208237671-59.919873668-7.1247152218126.6094982016
390.04966082493980.0209313740605-0.04628504439480.0344968261057-0.08149662687320.0920290805641-0.0937312453918-0.231461738971-0.245368594124-0.06416385291410.05638611178330.1165547618960.1478190245760.07416821821614.13108743241E-50.303335936067-0.06072426373440.04830635202010.2589533406610.07047194401980.238410756122-37.2405369802-4.3183605416726.5577665891
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 12 )AA1 - 121 - 12
22chain 'A' and (resid 13 through 29 )AA13 - 2913 - 31
33chain 'A' and (resid 30 through 44 )AA30 - 4432 - 50
44chain 'A' and (resid 45 through 63 )AA45 - 6351 - 69
55chain 'A' and (resid 64 through 82 )AA64 - 8270 - 88
66chain 'A' and (resid 83 through 89 )AA83 - 8989 - 95
77chain 'A' and (resid 90 through 104 )AA90 - 10496 - 110
88chain 'A' and (resid 105 through 123 )AA105 - 123111 - 129
99chain 'B' and (resid 2 through 12 )BB2 - 121 - 11
1010chain 'B' and (resid 13 through 35 )BB13 - 3512 - 36
1111chain 'B' and (resid 36 through 82 )BB36 - 8237 - 83
1212chain 'B' and (resid 83 through 123 )BB83 - 12384 - 126
1313chain 'C' and (resid 1 through 12 )CD1 - 121 - 12
1414chain 'C' and (resid 13 through 29 )CD13 - 2913 - 29
1515chain 'C' and (resid 30 through 35 )CD30 - 3530 - 35
1616chain 'C' and (resid 36 through 82 )CD36 - 8236 - 86
1717chain 'C' and (resid 83 through 123 )CD83 - 12387 - 129
1818chain 'D' and (resid 3 through 12 )DE3 - 121 - 10
1919chain 'D' and (resid 13 through 29 )DE13 - 2911 - 31
2020chain 'D' and (resid 30 through 35 )DE30 - 3532 - 37
2121chain 'D' and (resid 36 through 63 )DE36 - 6338 - 65
2222chain 'D' and (resid 64 through 73 )DE64 - 7366 - 75
2323chain 'D' and (resid 74 through 104 )DE74 - 10476 - 108
2424chain 'D' and (resid 105 through 123 )DE105 - 123109 - 127
2525chain 'E' and (resid 1 through 5 )EF1 - 5
2626chain 'E' and (resid 6 through 30 )EF6 - 30
2727chain 'E' and (resid 31 through 32 )EF31 - 32
2828chain 'F' and (resid 1 through 5 )FH1 - 5
2929chain 'F' and (resid 6 through 10 )FH6 - 10
3030chain 'F' and (resid 11 through 20 )FH11 - 20
3131chain 'F' and (resid 21 through 31 )FH21 - 31
3232chain 'G' and (resid 1 through 5 )GJ1 - 5
3333chain 'G' and (resid 6 through 10 )GJ6 - 10
3434chain 'G' and (resid 11 through 20 )GJ11 - 20
3535chain 'G' and (resid 21 through 32 )GJ21 - 32
3636chain 'H' and (resid 1 through 5 )HL1 - 5
3737chain 'H' and (resid 6 through 15 )HL6 - 15
3838chain 'H' and (resid 16 through 20 )HL16 - 20
3939chain 'H' and (resid 21 through 32 )HL21 - 32

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