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- PDB-7opr: Rab27a fusion with Slp2a-RBDa1 effector covalent adduct with CB1 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7opr | ||||||
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Title | Rab27a fusion with Slp2a-RBDa1 effector covalent adduct with CB1 in C123 | ||||||
![]() | Synaptotagmin-like protein 2,Ras-related protein Rab-27A | ||||||
![]() | EXOCYTOSIS / GTPase / Exosome / Acrylamide / Cysteine-reactive | ||||||
Function / homology | ![]() multivesicular body organization / cytotoxic T cell degranulation / positive regulation of constitutive secretory pathway / positive regulation of regulated secretory pathway / melanosome localization / natural killer cell degranulation / exosomal secretion / melanosome transport / Weibel-Palade body / neurexin family protein binding ...multivesicular body organization / cytotoxic T cell degranulation / positive regulation of constitutive secretory pathway / positive regulation of regulated secretory pathway / melanosome localization / natural killer cell degranulation / exosomal secretion / melanosome transport / Weibel-Palade body / neurexin family protein binding / exocytic vesicle / melanocyte differentiation / melanosome membrane / multivesicular body sorting pathway / RAB geranylgeranylation / myosin V binding / multivesicular body membrane / RAB GEFs exchange GTP for GDP on RABs / Regulation of MITF-M-dependent genes involved in pigmentation / complement-dependent cytotoxicity / vesicle docking involved in exocytosis / positive regulation of reactive oxygen species biosynthetic process / synaptic vesicle transport / Insulin processing / phosphatidylserine binding / exocytosis / positive regulation of exocytosis / antigen processing and presentation / protein secretion / photoreceptor outer segment / phosphatase binding / positive regulation of phagocytosis / vesicle-mediated transport / phosphatidylinositol-4,5-bisphosphate binding / small monomeric GTPase / G protein activity / secretory granule / intracellular protein transport / small GTPase binding / specific granule lumen / GDP binding / blood coagulation / melanosome / late endosome / lysosome / apical plasma membrane / protein domain specific binding / GTPase activity / dendrite / Neutrophil degranulation / positive regulation of gene expression / GTP binding / Golgi apparatus / extracellular exosome / extracellular region / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jamshidiha, M. / Tersa, M. / Lanyon-Hogg, T. / Perez-Dorado, I. / Sutherell, C.L. / De Vita, E. / Morgan, R.M.L. / Tate, E.W. / Cota, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Identification of the first structurally validated covalent ligands of the small GTPase RAB27A. Authors: Jamshidiha, M. / Lanyon-Hogg, T. / Sutherell, C.L. / Craven, G.B. / Tersa, M. / De Vita, E. / Brustur, D. / Perez-Dorado, I. / Hassan, S. / Petracca, R. / Morgan, R.M. / Sanz-Hernandez, M. / ...Authors: Jamshidiha, M. / Lanyon-Hogg, T. / Sutherell, C.L. / Craven, G.B. / Tersa, M. / De Vita, E. / Brustur, D. / Perez-Dorado, I. / Hassan, S. / Petracca, R. / Morgan, R.M. / Sanz-Hernandez, M. / Norman, J.C. / Armstrong, A. / Mann, D.J. / Cota, E. / Tate, E.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.9 KB | Display | ![]() |
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PDB format | ![]() | 85.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 22.9 KB | Display | |
Data in CIF | ![]() | 31.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7oppC ![]() 7opqC ![]() 3bc1S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 25857.162 Da / Num. of mol.: 2 / Mutation: Q78L,C188S Source method: isolated from a genetically manipulated source Details: The ligand CB1 is covalently bound to cysteine123. I have modelled the ligand CB1 already covalently bound to Cys123 into the peptide chain, for this reason there is a mismatch in the ...Details: The ligand CB1 is covalently bound to cysteine123. I have modelled the ligand CB1 already covalently bound to Cys123 into the peptide chain, for this reason there is a mismatch in the sequence. I am not sure how to fix this mismatch. I hope this information is clear.,The ligand CB1 is covalently bound to cysteine123. I have modelled the ligand CB1 already covalently bound to Cys123 into the peptide chain, for this reason there is a mismatch in the sequence. I am not sure how to fix this mismatch. I hope this information is clear. Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q9HCH5, UniProt: P51159, small monomeric GTPase |
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-Non-polymers , 5 types, 263 molecules ![](data/chem/img/GNP.gif)
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![](data/chem/img/HOH.gif)
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#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-GOL / #5: Chemical | ![]() Source method: isolated from a genetically manipulated source Formula: C17H18N4O Details: The ligand CB1 is covalently bound to cysteine123. I have modelled the ligand CB1 already covalently bound to Cys123 into the peptide chain, for this reason there is a mismatch in the ...Details: The ligand CB1 is covalently bound to cysteine123. I have modelled the ligand CB1 already covalently bound to Cys123 into the peptide chain, for this reason there is a mismatch in the sequence. I am not sure how to fix this mismatch. I hope this information is clear. Source: (gene. exp.) ![]() ![]() ![]() #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.28 % |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 0.15M Ammonium sulphate, 0.1M MES pH 6.0, 15% w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jul 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.32→64.33 Å / Num. obs: 49537 / % possible obs: 99.82 % / Redundancy: 2 % / Biso Wilson estimate: 32.9 Å2 / CC1/2: 0.993 / CC star: 0.998 / Rmerge(I) obs: 0.05896 / Rpim(I) all: 0.05896 / Rrim(I) all: 0.08338 / Net I/σ(I): 9.77 |
Reflection shell | Resolution: 2.32→2.403 Å / Redundancy: 2 % / Rmerge(I) obs: 0.3254 / Mean I/σ(I) obs: 2.23 / Num. unique obs: 4902 / CC1/2: 0.832 / CC star: 0.953 / Rpim(I) all: 0.3254 / Rrim(I) all: 0.4602 / % possible all: 99.67 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 3BC1 Resolution: 2.32→64.33 Å / SU ML: 0.29 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.65 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 108.06 Å2 / Biso mean: 36.1718 Å2 / Biso min: 11.48 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.32→64.33 Å
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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