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Open data
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Basic information
Entry | Database: PDB / ID: 7omq | ||||||
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Title | SaFtsZ complexed with GDPPCP and Mn2+ | ||||||
![]() | Cell division protein FtsZ | ||||||
![]() | CELL CYCLE / Cell division protein | ||||||
Function / homology | ![]() chloroplast fission / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fernandez-Tornero, C. / Ruiz, F.M. / Andreu, J.M. | ||||||
![]() | ![]() Title: FtsZ filament structures in different nucleotide states reveal the mechanism of assembly dynamics. Authors: Ruiz, F.M. / Huecas, S. / Santos-Aledo, A. / Prim, E.A. / Andreu, J.M. / Fernandez-Tornero, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 222.5 KB | Display | ![]() |
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PDB format | ![]() | 148 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 782.3 KB | Display | ![]() |
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Full document | ![]() | 782.8 KB | Display | |
Data in XML | ![]() | 8.5 KB | Display | |
Data in CIF | ![]() | 13.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ohhC ![]() 7ohkC ![]() 7ohlC ![]() 7ohnC ![]() 7oi2C ![]() 7ojaC ![]() 7ojbC ![]() 7ojcC ![]() 7ojdC ![]() 7ojzC ![]() 7omjC ![]() 7ompC ![]() 7on2C ![]() 7on3C ![]() 7on4C ![]() 6rvnS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 31940.100 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-GCP / | ||||
#3: Chemical | ChemComp-MN / | ||||
#4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.15 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop Details: 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 22% polyethylene glycol 5000 monomethyl ether (+ 20 mM MnCl2 for soaking, 23h) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 17, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.45→40.98 Å / Num. obs: 52628 / % possible obs: 98.68 % / Redundancy: 4.4 % / Biso Wilson estimate: 20.77 Å2 / CC1/2: 0.998 / Net I/σ(I): 9.88 |
Reflection shell | Resolution: 1.45→1.5 Å / Mean I/σ(I) obs: 1.11 / Num. unique obs: 5227 / CC1/2: 0.62 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6RVN Resolution: 1.45→40.98 Å / SU ML: 0.1857 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.5089 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.63 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.45→40.98 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION / Auth asym-ID: A / Label asym-ID: A
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