+Open data
-Basic information
Entry | Database: PDB / ID: 6rvn | ||||||
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Title | aFtsz-GDP-Wat | ||||||
Components | Cell division protein FtsZ | ||||||
Keywords | CELL CYCLE / Cell division protein | ||||||
Function / homology | Function and homology information chloroplast fission / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.242 Å | ||||||
Authors | Fernandez-Tornero, C. / Andreu, J.M. | ||||||
Citation | Journal: Febs J. / Year: 2020 Title: Nucleotide-induced folding of cell division protein FtsZ from Staphylococcus aureus. Authors: Huecas, S. / Canosa-Valls, A.J. / Araujo-Bazan, L. / Ruiz, F.M. / Laurents, D.V. / Fernandez-Tornero, C. / Andreu, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6rvn.cif.gz | 123.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6rvn.ent.gz | 95.3 KB | Display | PDB format |
PDBx/mmJSON format | 6rvn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6rvn_validation.pdf.gz | 759.6 KB | Display | wwPDB validaton report |
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Full document | 6rvn_full_validation.pdf.gz | 761.7 KB | Display | |
Data in XML | 6rvn_validation.xml.gz | 14.9 KB | Display | |
Data in CIF | 6rvn_validation.cif.gz | 21.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rv/6rvn ftp://data.pdbj.org/pub/pdb/validation_reports/rv/6rvn | HTTPS FTP |
-Related structure data
Related structure data | 6rvmC 6rvpC 6rvqC 6si9C 5mn4S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 31825.014 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: ftsZ / Production host: Escherichia coli (E. coli) / References: UniProt: P0A031 |
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#2: Chemical | ChemComp-GDP / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.33 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 25% PEG5K MME, 10% EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 8.5 |
-Data collection
Diffraction | Mean temperature: 273 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.9793 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 1, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.24→41.19 Å / Num. obs: 84186 / % possible obs: 99.9 % / Redundancy: 6.4 % / CC1/2: 0.993 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 1.24→1.31 Å / Num. unique obs: 12233 / CC1/2: 0.71 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5MN4 Resolution: 1.242→41.19 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 0.31 / Phase error: 24.72
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.242→41.19 Å
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Refine LS restraints |
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LS refinement shell |
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