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Open data
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Basic information
Entry | Database: PDB / ID: 7nyi | ||||||
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Title | BacSp222 bacteriocin: succinyl-K20 form | ||||||
![]() | Bacteriocin BacSp222 | ||||||
![]() | ANTIMICROBIAL PROTEIN / Peptide bacteriocin / succinylation | ||||||
Function / homology | killing of cells of another organism / defense response to bacterium / extracellular region / Bacteriocin BacSp222![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Nowakowski, M. / Mak, P. / Smialek, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure, Biosynthesis, and Biological Activity of Succinylated Forms of Bacteriocin BacSp222. Authors: Smialek, J. / Nowakowski, M. / Bzowska, M. / Bochenska, O. / Wlizlo, A. / Kozik, A. / Dubin, G. / Mak, P. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 333.3 KB | Display | ![]() |
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PDB format | ![]() | 289.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 265 KB | Display | ![]() |
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Full document | ![]() | 358.9 KB | Display | |
Data in XML | ![]() | 21 KB | Display | |
Data in CIF | ![]() | 31.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 6028.939 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() Plasmid details: Isolated from post-culture medium / References: UniProt: A0A0P0C3P7 |
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Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 0.5 mM BacSp222 bacteriocin: succinyl-K20 form, 100 mM [U-100% 2H] sodium acetate, 90 % H2O, 10 % D2O, 90% H2O/10% D2O Label: unlablled / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: sample_conditions_1 / pH: 5 / Pressure: 1 atm / Temperature: 298 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer | Type: Varian Uniform NMR System / Manufacturer: Varian / Model: Uniform NMR System / Field strength: 700 MHz |
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Processing
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Refinement | Method: simulated annealing / Software ordinal: 3 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 400 / Conformers submitted total number: 20 |