PDB-7nt5: CryoEM structure of the Nipah virus nucleocapsid single helical t...

ID or keywords:

Entry

Database: PDB / ID: 7nt5

Title

CryoEM structure of the Nipah virus nucleocapsid single helical turn assembly

Components

Nucleoprotein
RNA (78-MER)

Keywords

VIRAL PROTEIN / Protein-RNA complex / Nucleocapsid

Function / homology

Function and homology information

negative stranded viral RNA transcription / negative stranded viral RNA replication / helical viral capsid / viral nucleocapsid / host cell cytoplasm / molecular adaptor activity / ribonucleoprotein complex / structural molecule activity / RNA binding
Similarity search - Function

Biological species

Nipah virus

Escherichia coli BL21 (bacteria)

Method

ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å

Authors

Ker, D.S. / Jenkins, H.T. / Greive, S.J. / Antson, A.A.

Funding support

United Kingdom, 1items

Citation

Journal: PLoS Pathog / Year: 2021
Title: CryoEM structure of the Nipah virus nucleocapsid assembly.
Authors: De-Sheng Ker / Huw T Jenkins / Sandra J Greive / Alfred A Antson /

Abstract: Nipah and its close relative Hendra are highly pathogenic zoonotic viruses, storing their ssRNA genome in a helical nucleocapsid assembly formed by the N protein, a major viral immunogen. Here, we ...

History

Deposition	Mar 9, 2021	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jul 7, 2021	Provider: repository / Type: Initial release
Revision 1.1	Jul 28, 2021	Group: Database references / Category: citation Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

Movie	Deposited structure unit Imaged by Jmol Download Simplified surface model + fitted atomic model EMDB-12581 Imaged by Jmol Download Superimposition on EM map EMDB-12581 Imaged by UCSF Chimera Download Movie viewer
Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7nt5.cif.gz	1005.4 KB	Display	PDBx/mmCIF format
PDB format	pdb7nt5.ent.gz	835.5 KB	Display	PDB format
PDBx/mmJSON format	7nt5.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7nt5_validation.pdf.gz	1.4 MB	Display	wwPDB validaton report
Full document	7nt5_full_validation.pdf.gz	1.4 MB	Display
Data in XML	7nt5_validation.xml.gz	141.3 KB	Display
Data in CIF	7nt5_validation.cif.gz	219.5 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/nt/7nt5 ftp://data.pdbj.org/pub/pdb/validation_reports/nt/7nt5	HTTPS FTP

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Related structure data

Related structure data	12581MC 12582C 12583C 12584C 7nt6C M: map data used to model this data C: citing same article (ref.)
Similar structure data	12581 9945 10134 7dop-d 22076 4erp-1 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#121 - Jan 2010 70S Ribosomes similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #260 - Aug 2021 Ribonuclease P similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #253 - Jan 2021 Expressome similarity (1) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #15 - Mar 2001 Transfer RNA similarity (1) #10 - Oct 2000 Ribosome similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #65 - May 2005 Self-splicing RNA similarity (1) #245 - May 2020 Spliceosomes similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #105 - Sep 2008 Ribonuclease A similarity (1) #231 - Mar 2019 Measles Virus Proteins similarity (2) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #227 - Nov 2018 Telomerase similarity (1) #161 - May 2013 Ricin similarity (1) #143 - Nov 2011 Toll-like Receptors similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #62 - Feb 2005 Major Histocompatibility Complex similarity (2) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#121 - Jan 2010
70S Ribosomes
similarity (1)
#130 - Oct 2010
Riboswitches
similarity (1)
#16 - Apr 2001
Aminoacyl-tRNA Synthetases
similarity (1)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (1)
#181 - Jan 2015
Cascade and CRISPR
similarity (1)
#260 - Aug 2021
Ribonuclease P
similarity (1)
#229 - Jan 2019
Fluorescent RNA Aptamers
similarity (1)
#253 - Jan 2021
Expressome
similarity (1)
#146 - Feb 2012
Aminoglycoside Antibiotics
similarity (1)
#249 - Sep 2020
SARS-CoV-2 RNA-dependent RNA Polymerase
similarity (1)
#15 - Mar 2001
Transfer RNA
similarity (1)
#10 - Oct 2000
Ribosome
similarity (1)
#87 - Mar 2007
Zinc Fingers
similarity (1)
#210 - Jun 2017
Adenine Riboswitch in Action
similarity (1)
#98 - Feb 2008
Small Interfering RNA
similarity (1)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (1)
#65 - May 2005
Self-splicing RNA
similarity (1)
#245 - May 2020
Spliceosomes
similarity (1)
#226 - Oct 2018
Aminoglycoside Antibiotics and Resistance
similarity (1)
#105 - Sep 2008
Ribonuclease A
similarity (1)
#231 - Mar 2019
Measles Virus Proteins
similarity (2)
#230 - Feb 2019
Initiation Factor eIF4E
similarity (1)
#227 - Nov 2018
Telomerase
similarity (1)
#161 - May 2013
Ricin
similarity (1)
#143 - Nov 2011
Toll-like Receptors
similarity (1)
#106 - Oct 2008
Poly(A) Polymerase
similarity (1)
#266 - Feb 2022
Oligosaccharyltransferase
similarity (1)
#81 - Sep 2006
Elongation Factors
similarity (1)
#104 - Aug 2008
Selenocysteine Synthase
similarity (1)
#89 - May 2007
Aconitase and Iron Regulatory Protein 1
similarity (1)
#62 - Feb 2005
Major Histocompatibility Complex
similarity (2)
#241 - Jan 2020
Twenty Years of Molecules
similarity (1)

Deposited unit

A: Nucleoprotein

B: Nucleoprotein

C: Nucleoprotein

D: Nucleoprotein

E: Nucleoprotein

F: Nucleoprotein

G: Nucleoprotein

H: Nucleoprotein

I: Nucleoprotein

J: Nucleoprotein

K: Nucleoprotein

L: Nucleoprotein

M: Nucleoprotein

N: RNA (78-MER)

812 kDa, 14 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:

Dom-ID	Ens-ID	Auth asym-ID	Auth seq-ID
1	1	A	4 - 396
2	1	B	4 - 396
1	2	A	4 - 397
2	2	C	4 - 397
1	3	A	4 - 396
2	3	D	4 - 396
1	4	A	4 - 396
2	4	E	4 - 396
1	5	A	4 - 396
2	5	F	4 - 396
1	6	A	4 - 396
2	6	G	4 - 396
1	7	A	4 - 396
2	7	H	4 - 396
1	8	A	4 - 396
2	8	I	4 - 396
1	9	A	4 - 396
2	9	J	4 - 396
1	10	A	4 - 396
2	10	K	4 - 396
1	11	A	4 - 397
2	11	L	4 - 397
1	12	A	4 - 397
2	12	M	4 - 397
1	13	B	4 - 396
2	13	C	4 - 396
1	14	B	4 - 398
2	14	D	4 - 398
1	15	B	4 - 398
2	15	E	4 - 398
1	16	B	4 - 398
2	16	F	4 - 398
1	17	B	4 - 398
2	17	G	4 - 398
1	18	B	4 - 398
2	18	H	4 - 398
1	19	B	4 - 398
2	19	I	4 - 398
1	20	B	4 - 398
2	20	J	4 - 398
1	21	B	4 - 398
2	21	K	4 - 398
1	22	B	4 - 398
2	22	L	4 - 398
1	23	B	4 - 398
2	23	M	4 - 398
1	24	C	4 - 396
2	24	D	4 - 396
1	25	C	4 - 396
2	25	E	4 - 396
1	26	C	4 - 396
2	26	F	4 - 396
1	27	C	4 - 396
2	27	G	4 - 396
1	28	C	4 - 396
2	28	H	4 - 396
1	29	C	4 - 396
2	29	I	4 - 396
1	30	C	4 - 396
2	30	J	4 - 396
1	31	C	4 - 396
2	31	K	4 - 396
1	32	C	4 - 397
2	32	L	4 - 397
1	33	C	4 - 397
2	33	M	4 - 397
1	34	D	4 - 398
2	34	E	4 - 398
1	35	D	4 - 398
2	35	F	4 - 398
1	36	D	4 - 398
2	36	G	4 - 398
1	37	D	4 - 398
2	37	H	4 - 398
1	38	D	4 - 398
2	38	I	4 - 398
1	39	D	4 - 398
2	39	J	4 - 398
1	40	D	4 - 398
2	40	K	4 - 398
1	41	D	4 - 398
2	41	L	4 - 398
1	42	D	4 - 398
2	42	M	4 - 398
1	43	E	4 - 398
2	43	F	4 - 398
1	44	E	4 - 398
2	44	G	4 - 398
1	45	E	4 - 398
2	45	H	4 - 398
1	46	E	4 - 398
2	46	I	4 - 398
1	47	E	4 - 398
2	47	J	4 - 398
1	48	E	4 - 398
2	48	K	4 - 398
1	49	E	4 - 398
2	49	L	4 - 398
1	50	E	4 - 398
2	50	M	4 - 398
1	51	F	4 - 398
2	51	G	4 - 398
1	52	F	4 - 398
2	52	H	4 - 398
1	53	F	4 - 398
2	53	I	4 - 398
1	54	F	4 - 398
2	54	J	4 - 398
1	55	F	4 - 398
2	55	K	4 - 398
1	56	F	4 - 398
2	56	L	4 - 398
1	57	F	4 - 398
2	57	M	4 - 398
1	58	G	4 - 398
2	58	H	4 - 398
1	59	G	4 - 398
2	59	I	4 - 398
1	60	G	4 - 398
2	60	J	4 - 398
1	61	G	4 - 398
2	61	K	4 - 398
1	62	G	4 - 398
2	62	L	4 - 398
1	63	G	4 - 398
2	63	M	4 - 398
1	64	H	4 - 398
2	64	I	4 - 398
1	65	H	4 - 398
2	65	J	4 - 398
1	66	H	4 - 398
2	66	K	4 - 398
1	67	H	4 - 398
2	67	L	4 - 398
1	68	H	4 - 398
2	68	M	4 - 398
1	69	I	4 - 398
2	69	J	4 - 398
1	70	I	4 - 398
2	70	K	4 - 398
1	71	I	4 - 398
2	71	L	4 - 398
1	72	I	4 - 398
2	72	M	4 - 398
1	73	J	4 - 398
2	73	K	4 - 398
1	74	J	4 - 398
2	74	L	4 - 398
1	75	J	4 - 398
2	75	M	4 - 398
1	76	K	4 - 398
2	76	L	4 - 398
1	77	K	4 - 398
2	77	M	4 - 398
1	78	L	4 - 398
2	78	M	4 - 398

NCS ensembles :

#1: Protein	Nucleoprotein / Protein N / Nucleocapsid protein Mass: 60609.484 Da / Num. of mol.: 13 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nipah virus / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9IK92
#2: RNA chain	RNA (78-MER) Mass: 23835.986 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli BL21(DE3) (bacteria) / Production host: Escherichia coli BL21(DE3) (bacteria)

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Experiment

Experiment	Method: ELECTRON MICROSCOPY
EM experiment	Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component

ID	Name	Type	Entity ID	Parent-ID	Source
1	Nipah virus nucleocapsid Protein-RNA complex	COMPLEX	all	0	RECOMBINANT
2	Nucleoprotein	COMPLEX	#1	1	RECOMBINANT
3	RNA (78-MER)	COMPLEX	#2	1	RECOMBINANT

Molecular weight

Value: 1 MDa / Experimental value: NO

Source (natural)

ID	Entity assembly-ID	Organism	Ncbi tax-ID
1	2	Nipah henipavirus	121791
3	3	Escherichia coli BL21(DE3) (bacteria)	469008

Source (recombinant)

ID	Entity assembly-ID	Organism	Ncbi tax-ID
1	2	Escherichia coli BL21(DE3) (bacteria)	469008
2	3	Escherichia coli BL21(DE3) (bacteria)	469008

Buffer solution

pH: 8

Buffer component

ID	Conc.	Name	Formula	Buffer-ID
1	20 mM	Tris	C₄H₁₁NO₃	1
2	300 mM	sodium chloride	NaCl	1

Specimen

Conc.: 0.4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES

Vitrification

Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company
Microscopy	Model: FEI TITAN KRIOS
Electron gun	Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lens	Mode: BRIGHT FIELD / Nominal defocus max: 2100 nm / Nominal defocus min: 500 nm
Specimen holder	Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recording	Electron dose: 41 e/Å² / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 1879
Image scans	Movie frames/image: 40 / Used frames/image: 1-40

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Processing

Software

Name: REFMAC / Version: 5.8.0267 / Classification: refinement

EM software

ID	Name	Version	Category
1	RELION	3	particle selection
2	EPU		image acquisition
4	CTFFIND	4	CTF correction
7	UCSF Chimera	1.13	model fitting
11	RELION	3.1beta	classification
12	RELION	3.1beta	3D reconstruction
19	REFMAC	5.8.0267	model refinement
20	PHENIX	1.17	model refinement
21	ERRASER	2	model refinement
22	ISOLDE	0.9	model refinement

CTF correction

Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Particle selection

Num. of particles selected: 217522

Symmetry

Point symmetry: C₁ (asymmetric)

3D reconstruction

Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 124891
Details: Local symmetry was applied using the reconstruction
Num. of class averages: 5 / Symmetry type: POINT

Atomic model building

Protocol: RIGID BODY FIT

Atomic model building

PDB-ID: 4CO6

4co6

Pdb chain-ID: A / Pdb chain residue range: 32-369

Refinement

Resolution: 3.5→212.74 Å / Cor.coef. F_o:F_c: 0.94 / SU B: 34.89 / SU ML: 0.472 / ESU R: 0.633
Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES

	R_factor	Num. reflection	% reflection
R_work	0.31006	-	-
obs	0.31006	279030	100 %

Solvent computation

Solvent model: PARAMETERS FOR MASK CACLULATION

Displacement parameters

B_iso mean: 175.968 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-7.08 Å²	-1.11 Å²	3 Å²
2-	-	-6.22 Å²	-4.11 Å²
3-	-	-	13.3 Å²

Refinement step

Cycle: 1 / Total: 40200

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
ELECTRON MICROSCOPY	r_bond_refined_d	0.012	0.012	41016
ELECTRON MICROSCOPY	r_bond_other_d
ELECTRON MICROSCOPY	r_angle_refined_deg	2.213	1.621	55861
ELECTRON MICROSCOPY	r_angle_other_deg
ELECTRON MICROSCOPY	r_dihedral_angle_1_deg	7.786	5	5103
ELECTRON MICROSCOPY	r_dihedral_angle_2_deg	30.494	20.704	1846
ELECTRON MICROSCOPY	r_dihedral_angle_3_deg	15.904	15	6669
ELECTRON MICROSCOPY	r_dihedral_angle_4_deg	24.31	15	325
ELECTRON MICROSCOPY	r_chiral_restr	0.165	0.2	5757
ELECTRON MICROSCOPY	r_gen_planes_refined	0.014	0.02	29889
ELECTRON MICROSCOPY	r_gen_planes_other
ELECTRON MICROSCOPY	r_nbd_refined
ELECTRON MICROSCOPY	r_nbd_other
ELECTRON MICROSCOPY	r_nbtor_refined
ELECTRON MICROSCOPY	r_nbtor_other
ELECTRON MICROSCOPY	r_xyhbond_nbd_refined
ELECTRON MICROSCOPY	r_xyhbond_nbd_other
ELECTRON MICROSCOPY	r_metal_ion_refined
ELECTRON MICROSCOPY	r_metal_ion_other
ELECTRON MICROSCOPY	r_symmetry_vdw_refined
ELECTRON MICROSCOPY	r_symmetry_vdw_other
ELECTRON MICROSCOPY	r_symmetry_hbond_refined
ELECTRON MICROSCOPY	r_symmetry_hbond_other
ELECTRON MICROSCOPY	r_symmetry_metal_ion_refined
ELECTRON MICROSCOPY	r_symmetry_metal_ion_other
ELECTRON MICROSCOPY	r_mcbond_it	28.042	17.566	20457
ELECTRON MICROSCOPY	r_mcbond_other
ELECTRON MICROSCOPY	r_mcangle_it	41.647	26.227	25545
ELECTRON MICROSCOPY	r_mcangle_other
ELECTRON MICROSCOPY	r_scbond_it	37.599	17.915	20559
ELECTRON MICROSCOPY	r_scbond_other
ELECTRON MICROSCOPY	r_scangle_it
ELECTRON MICROSCOPY	r_scangle_other
ELECTRON MICROSCOPY	r_long_range_B_refined	64.403		176839
ELECTRON MICROSCOPY	r_long_range_B_other
ELECTRON MICROSCOPY	r_rigid_bond_restr
ELECTRON MICROSCOPY	r_sphericity_free
ELECTRON MICROSCOPY	r_sphericity_bonded

Refine LS restraints NCS

Refine-ID: ELECTRON MICROSCOPY / Type: interatomic distance / Weight position: 0.05

Ens-ID	Dom-ID	Auth asym-ID	Number	Rms dev position (Å)
1	1	A	22758	0.16
1	2	B	22758	0.16
2	1	A	22492	0.16
2	2	C	22492	0.16
3	1	A	22588	0.16
3	2	D	22588	0.16
4	1	A	22662	0.17
4	2	E	22662	0.17
5	1	A	22546	0.16
5	2	F	22546	0.16
6	1	A	22634	0.16
6	2	G	22634	0.16
7	1	A	22846	0.15
7	2	H	22846	0.15
8	1	A	22456	0.17
8	2	I	22456	0.17
9	1	A	22832	0.16
9	2	J	22832	0.16
10	1	A	22586	0.16
10	2	K	22586	0.16
11	1	A	22608	0.16
11	2	L	22608	0.16
12	1	A	22296	0.17
12	2	M	22296	0.17
13	1	B	22950	0.15
13	2	C	22950	0.15
14	1	B	23048	0.15
14	2	D	23048	0.15
15	1	B	22904	0.16
15	2	E	22904	0.16
16	1	B	23206	0.15
16	2	F	23206	0.15
17	1	B	23274	0.14
17	2	G	23274	0.14
18	1	B	23420	0.14
18	2	H	23420	0.14
19	1	B	22900	0.16
19	2	I	22900	0.16
20	1	B	23226	0.15
20	2	J	23226	0.15
21	1	B	23010	0.16
21	2	K	23010	0.16
22	1	B	23082	0.14
22	2	L	23082	0.14
23	1	B	22530	0.16
23	2	M	22530	0.16
24	1	C	22918	0.15
24	2	D	22918	0.15
25	1	C	22546	0.17
25	2	E	22546	0.17
26	1	C	22862	0.15
26	2	F	22862	0.15
27	1	C	22734	0.16
27	2	G	22734	0.16
28	1	C	23182	0.14
28	2	H	23182	0.14
29	1	C	22594	0.16
29	2	I	22594	0.16
30	1	C	22864	0.15
30	2	J	22864	0.15
31	1	C	22804	0.15
31	2	K	22804	0.15
32	1	C	22726	0.16
32	2	L	22726	0.16
33	1	C	22096	0.17
33	2	M	22096	0.17
34	1	D	22904	0.15
34	2	E	22904	0.15
35	1	D	23076	0.14
35	2	F	23076	0.14
36	1	D	23134	0.14
36	2	G	23134	0.14
37	1	D	23176	0.15
37	2	H	23176	0.15
38	1	D	22866	0.16
38	2	I	22866	0.16
39	1	D	23112	0.15
39	2	J	23112	0.15
40	1	D	22956	0.15
40	2	K	22956	0.15
41	1	D	22876	0.15
41	2	L	22876	0.15
42	1	D	22450	0.16
42	2	M	22450	0.16
43	1	E	23008	0.16
43	2	F	23008	0.16
44	1	E	23092	0.15
44	2	G	23092	0.15
45	1	E	23046	0.15
45	2	H	23046	0.15
46	1	E	22856	0.16
46	2	I	22856	0.16
47	1	E	22860	0.16
47	2	J	22860	0.16
48	1	E	23030	0.16
48	2	K	23030	0.16
49	1	E	22650	0.16
49	2	L	22650	0.16
50	1	E	22184	0.17
50	2	M	22184	0.17
51	1	F	23512	0.14
51	2	G	23512	0.14
52	1	F	23448	0.14
52	2	H	23448	0.14
53	1	F	22960	0.16
53	2	I	22960	0.16
54	1	F	23186	0.15
54	2	J	23186	0.15
55	1	F	23086	0.15
55	2	K	23086	0.15
56	1	F	23118	0.15
56	2	L	23118	0.15
57	1	F	22434	0.16
57	2	M	22434	0.16
58	1	G	23406	0.14
58	2	H	23406	0.14
59	1	G	23054	0.15
59	2	I	23054	0.15
60	1	G	23364	0.15
60	2	J	23364	0.15
61	1	G	23272	0.14
61	2	K	23272	0.14
62	1	G	23146	0.15
62	2	L	23146	0.15
63	1	G	22560	0.16
63	2	M	22560	0.16
64	1	H	23088	0.15
64	2	I	23088	0.15
65	1	H	23362	0.14
65	2	J	23362	0.14
66	1	H	23392	0.14
66	2	K	23392	0.14
67	1	H	23234	0.14
67	2	L	23234	0.14
68	1	H	22636	0.16
68	2	M	22636	0.16
69	1	I	22942	0.16
69	2	J	22942	0.16
70	1	I	23050	0.16
70	2	K	23050	0.16
71	1	I	22766	0.16
71	2	L	22766	0.16
72	1	I	22138	0.17
72	2	M	22138	0.17
73	1	J	23364	0.14
73	2	K	23364	0.14
74	1	J	23110	0.15
74	2	L	23110	0.15
75	1	J	22522	0.16
75	2	M	22522	0.16
76	1	K	22956	0.15
76	2	L	22956	0.15
77	1	K	22492	0.16
77	2	M	22492	0.16
78	1	L	22442	0.16
78	2	M	22442	0.16

LS refinement shell

Resolution: 3.5→3.591 Å / Total num. of bins used: 20

	R_factor	Num. reflection	% reflection
R_free	0	0	-
R_work	0.645	20718	-
obs	-	-	100 %

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