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Yorodumi- PDB-7nqc: Calmodulin extracts the Ras family protein RalA from lipid bilaye... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7nqc | ||||||
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Title | Calmodulin extracts the Ras family protein RalA from lipid bilayers by engagement with two membrane targeting motifs | ||||||
Components |
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Keywords | SIGNALING PROTEIN / farnesylation / calcium binding | ||||||
Function / homology | Function and homology information establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / establishment of protein localization to membrane / regulation of synaptic vesicle endocytosis / organelle localization by membrane tethering / regulation of synaptic vesicle exocytosis / regulation of cardiac muscle cell action potential / mitochondrion-endoplasmic reticulum membrane tethering / autophagosome membrane docking / nitric-oxide synthase binding ...establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / establishment of protein localization to membrane / regulation of synaptic vesicle endocytosis / organelle localization by membrane tethering / regulation of synaptic vesicle exocytosis / regulation of cardiac muscle cell action potential / mitochondrion-endoplasmic reticulum membrane tethering / autophagosome membrane docking / nitric-oxide synthase binding / protein phosphatase activator activity / adenylate cyclase binding / catalytic complex / calcium channel regulator activity / detection of calcium ion / regulation of cardiac muscle contraction / negative regulation of ryanodine-sensitive calcium-release channel activity / calcium channel inhibitor activity / cellular response to interferon-beta / phosphatidylinositol 3-kinase binding / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / positive regulation of protein dephosphorylation / regulation of ryanodine-sensitive calcium-release channel activity / titin binding / voltage-gated potassium channel complex / sperm midpiece / calcium channel complex / response to amphetamine / adenylate cyclase activator activity / nitric-oxide synthase regulator activity / regulation of heart rate / sarcomere / protein serine/threonine kinase activator activity / regulation of cytokinesis / positive regulation of receptor signaling pathway via JAK-STAT / spindle microtubule / cellular response to type II interferon / spindle pole / response to calcium ion / G2/M transition of mitotic cell cycle / calcium-dependent protein binding / myelin sheath / growth cone / transmembrane transporter binding / protein domain specific binding / centrosome / calcium ion binding / protein kinase binding / protein-containing complex / mitochondrion / nucleoplasm / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Chamberlain, S.G. / Owen, D. / Mott, H.R. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2021 Title: Calmodulin extracts the Ras family protein RalA from lipid bilayers by engagement with two membrane-targeting motifs. Authors: Chamberlain, S.G. / Gohlke, A. / Shafiq, A. / Squires, I.J. / Owen, D. / Mott, H.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7nqc.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7nqc.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 7nqc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7nqc_validation.pdf.gz | 326.3 KB | Display | wwPDB validaton report |
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Full document | 7nqc_full_validation.pdf.gz | 714.8 KB | Display | |
Data in XML | 7nqc_validation.xml.gz | 124.9 KB | Display | |
Data in CIF | 7nqc_validation.cif.gz | 151.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nq/7nqc ftp://data.pdbj.org/pub/pdb/validation_reports/nq/7nqc | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18572.418 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Calm1, Calm, Cam, Cam1, CaMI / Plasmid: pQE / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0DP29 | ||||
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#2: Protein/peptide | Mass: 2177.687 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RalA / Production host: Escherichia coli BL21 (bacteria) / References: small monomeric GTPase | ||||
#3: Chemical | ChemComp-CA / #4: Chemical | ChemComp-ULW / [( | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 109 mM / Label: 1 / pH: 6.7 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 30 |