+Open data
-Basic information
Entry | Database: PDB / ID: 7nji | ||||||
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Title | HEX1 (in cellulo) loaded on HARE serial crystallography chip | ||||||
Components | Woronin body major protein | ||||||
Keywords | STRUCTURAL PROTEIN / serial crystallography / HEX1 / in-cellulo / silicon chip | ||||||
Function / homology | Function and homology information Woronin body / positive regulation of translational termination / positive regulation of translational elongation / cell septum / translational elongation / translation elongation factor activity / ribosome binding / RNA binding Similarity search - Function | ||||||
Biological species | Neurospora crassa (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Norton-Baker, B. / Mehrabi, P. / Boger, J. / Schonherr, R. / von Stetten, D. / Schikora, H. / Martin, R.W. / Miller, R.J.D. / Redecke, L. / Schulz, E.C. | ||||||
Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2021 Title: A simple vapor-diffusion method enables protein crystallization inside the HARE serial crystallography chip. Authors: Norton-Baker, B. / Mehrabi, P. / Boger, J. / Schonherr, R. / von Stetten, D. / Schikora, H. / Kwok, A.O. / Martin, R.W. / Miller, R.J.D. / Redecke, L. / Schulz, E.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7nji.cif.gz | 47.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7nji.ent.gz | 28.6 KB | Display | PDB format |
PDBx/mmJSON format | 7nji.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7nji_validation.pdf.gz | 300.3 KB | Display | wwPDB validaton report |
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Full document | 7nji_full_validation.pdf.gz | 300.1 KB | Display | |
Data in XML | 7nji_validation.xml.gz | 7.7 KB | Display | |
Data in CIF | 7nji_validation.cif.gz | 9.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nj/7nji ftp://data.pdbj.org/pub/pdb/validation_reports/nj/7nji | HTTPS FTP |
-Related structure data
Related structure data | 7njeC 7njfC 7njgC 7njhC 7njjC 7nkfC 1khiS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 19150.664 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (fungus) Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987 Gene: hex-1, NCU08332 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P87252 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.25 % |
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Crystal grow | Temperature: 300 K / Method: in cell Details: Grown inside Spodoptera frugiperda Sf9 insect cells |
-Data collection
Diffraction | Mean temperature: 293 K / Serial crystal experiment: Y |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.9762 Å |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: May 1, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9762 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→86.94 Å / Num. obs: 9522 / % possible obs: 100 % / Redundancy: 93 % / Biso Wilson estimate: 40.33 Å2 / CC1/2: 0.9087 / Net I/σ(I): 3.2 |
Reflection shell | Resolution: 2.3→2.382 Å / Num. unique obs: 917 / CC1/2: 0.3817 |
Serial crystallography sample delivery | Method: fixed target |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1KHI Resolution: 2.3→51.01 Å / SU ML: 0.3218 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.012 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.52 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→51.01 Å
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Refine LS restraints |
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LS refinement shell |
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