PDB-7mrx: Cryogenic crystal structure of barnase A43C/S80C bound to barstar...

ID or keywords:

Entry

Database: PDB / ID: 7mrx

Title

Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A

Components

Barstar
Ribonuclease

Keywords

RNA BINDING PROTEIN/Inhibitor /

Disulfides /

Toxin /

antitoxin / RNA BINDING PROTEIN-Inhibitor complex

Function / homology

Function and homology information

Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity /

RNA binding / extracellular region /

cytoplasm
Similarity search - Function

Biological species

Bacillus amyloliquefaciens (bacteria)

Method

X-RAY DIFFRACTION /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 2.29 Å

Authors

Caro, J.A. / Smith, J. / Wand, A.J.

Citation

Journal: To Be Published
Title: Conformational entropy and protein affinity
Authors: Caro, J.A. / Wand, A.J.

History

Deposition	May 10, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	May 18, 2022	Provider: repository / Type: Initial release
Revision 1.1	Oct 18, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7mrx.cif.gz	262.2 KB	Display	PDBx/mmCIF format
PDB format	pdb7mrx.ent.gz	212.3 KB	Display	PDB format
PDBx/mmJSON format	7mrx.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/mr/7mrx ftp://data.pdbj.org/pub/pdb/validation_reports/mr/7mrx	HTTPS FTP

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Related structure data

Related structure data	2za4S 6pqk S: Starting model for refinement
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: Ribonuclease

B: Barstar

C: Ribonuclease

D: Barstar

E: Ribonuclease

F: Barstar

74.3 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Ribonuclease

B: Barstar

defined by author
Evidence: gel filtration, Final purification step was size-exclusion chromatography (S75) by gel filtration reporting an elution volume corresponding to the heterodimer, with no evidence of monomer.
24.8 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Ribonuclease

D: Barstar

defined by author
24.8 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

E: Ribonuclease

F: Barstar

defined by author
24.8 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	50.317, 87.860, 162.492
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁

#1: Protein	Ribonuclease / / Barnase / RNase Ba Mass: 14478.056 Da / Num. of mol.: 3 / Mutation: A43C, S80C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Production host: Escherichia coli (E. coli) References: UniProt: P00648, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters
#2: Protein	Barstar / / Ribonuclease inhibitor Mass: 10288.608 Da / Num. of mol.: 3 / Mutation: C41A, C83A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P11540
#3: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 361 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.63 Å³/Da / Density % sol: 50.69 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD PH range: 6-9

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Data collection

Diffraction

Mean temperature: 80 K
Ambient temp details: Collection under stream of liquid nitrogen
Serial crystal experiment: N

Diffraction source

Source:

ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54 Å

Detector

Type: RIGAKU SATURN 944+ / Detector: CCD / Date: May 1, 2016 / Details: Osmic VariMax mirror optics

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 1.54 Å / Relative weight: 1

Reflection

Resolution: 2.29→50 Å / Num. obs: 33166 / % possible obs: 99.7 % / Redundancy: 4.8 % / B_iso Wilson estimate: 34.12 Å² / R_merge(I) obs: 0.066 / Χ²: 2.09 / Net I/σ(I): 15.5 / Num. measured all: 158662

Reflection shell

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Num. unique obs	Χ²	Diffraction-ID	% possible all
2.29-2.33	3.3	0.243	1598	1.128	1	99.3
2.33-2.37	4.2	0.242	1625	1.223	1	99.8
2.37-2.42	4.8	0.221	1647	1.284	1	99.8
2.42-2.47	5.2	0.224	1625	1.214	1	100
2.47-2.52	5.2	0.197	1654	1.314	1	99.9
2.52-2.58	5.1	0.181	1612	1.388	1	99.9
2.58-2.64	5.1	0.162	1655	1.474	1	100
2.64-2.72	5.1	0.147	1621	1.601	1	100
2.72-2.79	5.1	0.128	1655	1.781	1	99.9
2.79-2.89	5	0.115	1632	2.005	1	99.8
2.89-2.99	5.1	0.102	1643	2.184	1	99.7
2.99-3.11	5	0.088	1639	2.432	1	99.9
3.11-3.25	4.9	0.08	1674	2.578	1	99.7
3.25-3.42	4.8	0.067	1647	2.684	1	99.8
3.42-3.63	4.7	0.059	1665	2.867	1	99.6
3.63-3.92	4.6	0.053	1678	2.899	1	99.6
3.92-4.31	4.5	0.048	1655	2.945	1	99.7
4.31-4.93	4.4	0.044	1698	2.879	1	99.6
4.93-6.21	4.5	0.045	1724	2.739	1	99.7
6.21-50	5	0.042	1819	2.957	1	98.5

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Phasing

Phasing

Method:

molecular replacement

Phasing MR

Model details: Phaser MODE: MR_AUTO

	Highest resolution	Lowest resolution
Rotation	3 Å	29.83 Å
Translation	3 Å	29.83 Å

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Processing

Software

Name	Version	Classification
PHENIX	1.19.1	refinement
HKL-2000		data reduction
SCALEPACK		data scaling
PHASER	2.5.5	phasing
PDB_EXTRACT	3.27	data extraction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 2za4

2za4

Resolution: 2.29→29.83 Å / SU ML: 0.29 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 22.85 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2293	1676	5.08 %
R_work	0.1828	31300	-
obs	0.1852	32976	99.03 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 115.56 Å² / B_iso mean: 44.103 Å² / B_iso min: 21.81 Å²

Refinement step

Cycle: final / Resolution: 2.29→29.83 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	4741	0	0	361	5102
B_iso mean	-	-	-	38.36	-
Num. residues	-	-	-	-	591

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 12

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
2.29-2.36	0.2968	153	0.2251	2421	2574	95
2.36-2.43	0.3117	146	0.2207	2571	2717	100
2.43-2.52	0.3008	152	0.2301	2601	2753	100
2.52-2.62	0.2777	133	0.2296	2566	2699	99
2.62-2.74	0.2982	148	0.225	2574	2722	99
2.74-2.88	0.2942	151	0.2294	2563	2714	99
2.88-3.06	0.3063	128	0.2047	2594	2722	99
3.06-3.3	0.2479	143	0.2097	2608	2751	99
3.3-3.63	0.2048	133	0.188	2638	2771	99
3.63-4.16	0.2024	121	0.1533	2660	2781	99
4.16-5.23	0.1577	137	0.1391	2666	2803	100
5.23-29.83	0.1767	131	0.1551	2838	2969	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.3007	-0.1261	0.7577	3.5566	-1.0613	2.859	0.12	-0.1185	-0.3726	0.1351	0.0285	-0.1963	0.406	0.2142	-0.1311	0.3424	-0.0112	-0.0868	0.3746	0.0787	0.2764	-14.3136	-48.9172	30.869
2	3.0792	-0.7086	0.4619	2.426	-0.8195	2.9229	0.0232	-0.1493	-0.6177	0.1418	0.0026	0.2461	0.4076	-0.1846	-0.282	0.366	-0.0821	-0.086	0.356	0.0998	0.4259	-22.1744	-49.6065	26.3578
3	4.7517	-0.5765	-0.0782	2.024	-0.576	2.7056	0.2418	0.198	-0.0129	-0.293	0.2804	0.8842	-0.0572	-0.4038	-0.4	0.3018	-0.0363	-0.0829	0.3242	0.096	0.4222	-31.6817	-43.099	18.9047
4	3.5793	0.5473	0.1264	2.1008	-0.6996	4.4822	0.2997	-0.0445	0.3679	0.0156	0.2608	0.4677	-0.3798	-0.5572	-0.2882	0.2744	0.0905	-0.0103	0.3983	0.0883	0.3858	-32.102	-33.3222	21.0839
5	3.0625	1.0096	0.462	1.8449	-1.662	4.0066	0.0918	-0.7245	-0.2361	0.5376	0.2879	0.4196	-0.3303	-0.2682	-0.3314	0.3102	0.0432	0.0577	0.4218	0.1267	0.2848	-30.9284	-39.3246	28.7352
6	2.1135	-0.4255	0.4524	1.5813	-0.8846	2.2182	0.1493	0.2789	-0.3503	-0.3537	-0.001	0.0678	0.4858	0.209	-0.1212	0.335	0.0739	-0.0223	0.316	-0.0122	0.291	-16.6951	-42.9552	13.5675
7	2.8531	-0.1231	0.3035	2.1683	-1.1377	3.0808	0.2781	0.4044	-0.3885	-0.5132	-0.2889	-0.1441	0.5806	0.2628	-0.0512	0.4793	0.1304	-0.0774	0.3229	-0.0075	0.2743	-10.9095	-47.7843	14.5513
8	2.5644	-0.7654	0.6702	2.7659	-0.9094	2.3773	0.0921	-0.1281	0.2589	0.2396	0.053	-0.1795	0.0067	0.0627	-0.0857	0.2806	-0.0093	-0.0625	0.2941	0.0296	0.2292	-18.6447	-37.8871	24.8013
9	1.8367	-0.2476	0.53	2.6403	-1.0222	1.9907	0.2408	-0.0704	-0.1074	-0.1482	-0.1466	-0.0836	0.2936	0.6969	-0.0199	0.3498	0.1133	-0.0759	0.3632	0.0508	0.3227	-8.8836	-48.5356	20.8904
10	2.5077	-0.0076	0.5478	2.6356	-1.4506	3.4712	0.1694	0.099	0.0733	0.1122	-0.1615	-0.3709	0.2248	0.1251	0.1152	0.318	-0.0299	-0.1115	0.3407	0.0219	0.2987	-8.1714	-38.5951	20.7699
11	7.2147	0.9665	-1.2151	0.2474	-0.1555	0.3176	0.0901	0.0385	0.5131	-0.3546	0.0098	0.2448	-0.1627	-0.2252	-0.0584	0.3283	-0.0418	0.0205	0.4218	0.0762	0.3107	-21.1787	-20.8389	2
12	2.4971	0.4412	-0.0784	2.1897	-0.449	2.7372	0.1068	0.001	0.007	0.3327	0.0792	-0.0948	-0.1429	0.1052	-0.1651	0.3003	0.0132	-0.0427	0.3015	0.0345	0.2364	-17.5518	-28.2776	11.5651
13	3.166	0.561	0.1011	2.3831	-0.5133	2.7913	-0.0645	0.5412	0.0616	-0.2356	0.2793	-0.0649	-0.2256	-0.099	-0.1642	0.3528	-0.0158	-0.0181	0.3348	0.0513	0.2679	-20.4496	-26.7547	0.7314
14	4.2	1.5254	-0.2621	3.2735	-0.7364	2.4843	0.0038	0.3241	-0.5345	0.1185	0.2913	-0.2612	0.9165	0.083	-0.2077	0.366	0.0472	-0.0616	0.3323	0.0053	0.2701	-20.6875	-35.7116	1.5843
15	3.0645	1.056	-0.4963	2.9659	-0.7948	3.6008	0.1617	-0.0947	-0.3225	-0.0381	0.0499	0.0558	0.2739	-0.2216	-0.2748	0.3514	-0.0089	-0.0367	0.4409	0.0775	0.3202	-28.8264	-32.8913	1.4114
16	3.2069	-0.2653	0.6711	2.6992	0.1248	2.9169	0.1211	-0.3477	0.2975	0.1568	0.0419	-0.3907	0.1607	0.4294	-0.0802	0.3011	0.0137	-0.0301	0.3892	-0.0962	0.2912	14.7343	-4.7635	42.427
17	3.4721	1.124	0.3559	3.6507	-0.046	2.019	0.066	0.1143	-0.0636	0.0655	0.076	-0.3018	0.4401	0.3173	-0.2049	0.3808	0.0611	-0.0506	0.3513	-0.0443	0.3101	15.156	-12.2859	37.5034
18	1.8134	0.4237	0.2855	6.5738	-0.7195	2.5689	0.3221	0.4618	-0.691	-0.4552	0.0502	0.0481	0.8971	-0.1183	-0.3459	0.4069	0.063	-0.1282	0.3834	-0.0435	0.4196	8.3395	-20.8104	29.2972
19	2.9334	0.0185	0.8809	3.1911	0.0867	3.0431	0.2799	-0.077	-0.6528	-0.2119	0.052	0.1295	0.9333	-0.1327	-0.2688	0.4382	-0.0427	-0.1325	0.274	0.0036	0.3714	3.6582	-18.3717	33.8237
20	2.6498	0.0582	0.5568	3.1995	0.0544	2.8934	-0.11	-0.0105	-0.0225	-0.3067	0.066	-0.0145	0.0804	0.0986	-0.0415	0.2704	-0.0031	-0.0182	0.2868	-0.0231	0.2172	8.571	-4.0013	29.1947
21	2.9317	0.0491	0.876	2.6778	0.2252	2.6507	-0.1913	0.3381	0.3308	-0.0514	0.0579	-0.1471	-0.2549	-0.0149	0.0311	0.3107	-0.0101	-0.0369	0.2789	-0.0447	0.252	10.2971	1.2495	32.224
22	0.5383	0.3159	-0.5299	2.0597	1.5996	2.5187	0.2418	0.1763	0.0582	-0.3822	-0.0039	1.29	-0.8143	-0.9125	0.2605	0.4888	0.1527	-0.036	0.6272	-0.0586	0.4612	-12.7003	-6.9303	11.9622
23	3.8749	0.8413	-0.1942	3.6902	2.5852	4.4675	0.0473	0.1858	0.097	-0.1752	0.0787	0.3897	-0.5499	-1.1845	0.0957	0.3423	0.092	-0.0473	0.5586	-0.0233	0.3304	-8.3245	-3.5345	22.1796
24	2.6324	0.128	0.1184	3.7897	2.8808	3.6792	0.1822	-0.7652	-0.0064	0.2198	-0.2892	0.0589	0.2515	-0.707	-0.1715	0.2952	-0.0546	-0.0574	0.4288	-0.0122	0.1985	-0.5969	-11.4023	22.7567
25	3.4191	-0.2624	0.0561	2.6757	1.4412	4.3286	0.21	0.9454	0.7573	-0.9136	-0.0786	0.2798	-0.9357	-0.6292	0.0336	0.6742	0.1621	-0.006	0.7245	0.1662	0.3891	-7.1156	-3.6438	7.0607
26	5.2621	-0.7753	-0.536	4.5437	2.7705	5.7587	0.013	0.0213	0.1111	-0.7407	0.1922	-0.41	-0.6196	-0.279	-0.3177	0.3455	-0.0219	0.0006	0.3975	-0.0119	0.2391	2.5008	-7.943	12.3798
27	4.3071	-1.0902	0.8607	4.2718	1.1579	6.3776	-0.0599	-0.1919	0.0159	0.1929	0.3165	-0.4501	-0.0084	-0.1939	-0.1267	0.2872	-0.0653	-0.0247	0.3373	0.0129	0.2277	-1.5692	-15.5262	11.2598
28	1.9153	-0.0493	-0.7899	2.1422	-0.5593	2.273	0.1795	0.0999	0.8792	-0.6728	-0.3446	-0.9395	-0.4189	-0.0146	0.0531	0.3985	0.0465	0.0476	0.3045	0.0862	0.9996	-13.7346	-11.5651	42.42
29	-0.0077	0.1991	0.1767	1.781	0.6763	0.7623	-0.4604	-0.4329	1.6665	1.1361	-0.2957	-0.8741	-0.396	0.3664	-0.7259	0.5695	-0.1846	-0.5283	-0.1754	-0.6232	1.4796	-6.792	-11.5567	58.1983
30	1.019	-0.9444	-0.0217	4.4864	-0.1898	1.5856	-0.1483	-0.1426	0.7432	0.2625	0.0838	-0.0745	-0.0221	0.002	0.0477	0.2502	0.0264	-0.0314	0.2629	-0.0512	0.5981	-16.6639	-22.2178	50.0585
31	1.6597	-1.6445	0.3992	2.2805	-1.7866	3.064	-0.3457	-1.2154	-0.0673	1.3997	0.551	0.8266	0.431	-0.4494	-0.0944	1.1047	0.1944	0.2061	1.2433	0.0793	0.6113	-19.1089	-36.1876	76.7005
32	3.3392	-2.7324	-0.4505	2.7564	-0.8928	3.0959	-0.1842	-1.2847	0.3107	1.413	0.1804	0.7319	-0.2755	-0.1352	0.1155	0.7768	0.048	0.2796	0.7445	-0.04	0.5179	-20.6274	-29.8133	71.154
33	3.1238	-1.509	0.4939	3.4393	0.3673	3.7531	-0.551	-0.5772	0.761	1.1822	0.304	0.0925	-0.5832	-0.1433	0.1436	0.6572	0.0189	0.0249	0.4349	-0.1735	0.5472	-16.5179	-23.7407	63.9107
34	4.1306	-0.8201	-0.2664	1.5784	-0.0038	1.4371	-0.8476	-1.4978	0.051	1.5362	0.1661	-0.2623	0.0685	0.1984	-0.0506	1.1746	0.277	-0.1172	1.0395	-0.2532	0.5679	-9.86	-26.9256	74.1466
35	1.9954	-1.4597	-0.6286	1.9181	-1.144	3.3543	-0.3647	-1.2158	-0.7572	1.2114	0.3037	0.6966	0.7164	-0.1751	-0.0762	1.0393	0.27	0.4185	0.8474	0.138	0.8134	-15.7287	-43.6821	70.4244
36	4.7344	-1.5633	1.375	4.5027	0.3468	4.9053	-0.3713	-0.3233	0.9235	0.7303	0.5037	-0.6205	-0.0663	0.5691	0.0929	0.5352	0.0637	-0.0916	0.5871	-0.1877	0.4616	-7.3001	-32.8844	64.4507
37	2.3695	-1.944	1.0416	2.228	0.3155	2.632	-0.4088	-0.8623	0.5561	1.3383	0.3338	-0.2926	-0.2192	0.6136	0.1536	0.8935	0.0465	-0.2014	1.1673	-0.179	0.6049	-4.0339	-30.2332	72.8725

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 3 through 17 )	A	3 - 17
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 18 through 26 )	A	18 - 26
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 27 through 33 )	A	27 - 33
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 34 through 41 )	A	34 - 41
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 42 through 50 )	A	42 - 50
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 51 through 60 )	A	51 - 60
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 61 through 75 )	A	61 - 75
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 76 through 91 )	A	76 - 91
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 92 through 99 )	A	92 - 99
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 100 through 110 )	A	100 - 110
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 0 through 12 )	B	0 - 12
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 13 through 43 )	B	13 - 43
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 44 through 65 )	B	44 - 65
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 66 through 79 )	B	66 - 79
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 80 through 89 )	B	80 - 89
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 3 through 17 )	C	3 - 17
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 18 through 26 )	C	18 - 26
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 27 through 33 )	C	27 - 33
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 34 through 55 )	C	34 - 55
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 56 through 86 )	C	56 - 86
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 87 through 110 )	C	87 - 110
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 0 through 12 )	D	0 - 12
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 13 through 33 )	D	13 - 33
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 34 through 48 )	D	34 - 48
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 49 through 65 )	D	49 - 65
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 66 through 79 )	D	66 - 79
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 80 through 89 )	D	80 - 89
28	X-RAY DIFFRACTION	28	chain 'E' and (resid 3 through 22 )	E	3 - 22
29	X-RAY DIFFRACTION	29	chain 'E' and (resid 23 through 56 )	E	23 - 56
30	X-RAY DIFFRACTION	30	chain 'E' and (resid 57 through 110 )	E	57 - 110
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 3 through 12 )	F	3 - 12
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 13 through 23 )	F	13 - 23
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 24 through 43 )	F	24 - 43
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 44 through 55 )	F	44 - 55
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 56 through 65 )	F	56 - 65
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 66 through 80 )	F	66 - 80
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 81 through 89 )	F	81 - 89

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