National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
HHSN272201200026C
米国
引用
ジャーナル: to be published タイトル: The crystal structure of Bacillus anthracis prephenate dehydrogenase identified an ACT regulatory domain and a novel mode of metabolic regulation for proteins within the prephenate ...タイトル: The crystal structure of Bacillus anthracis prephenate dehydrogenase identified an ACT regulatory domain and a novel mode of metabolic regulation for proteins within the prephenate dehydrogenase family of enzyme 著者: Shabalin, I.G. / Hou, J. / Christendat, D. / Minor, W.
温度: 289 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.5 詳細: 0.2 ul of 6 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 5% Glycerol, 10 mM BME, 5 mM NAD and 5 mM Tyrosine were mixed with 0.2 ul of the MCSG Suite 2 condition #46 (20%w/v PEG 3350, 0. ...詳細: 0.2 ul of 6 mg/ml protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 5% Glycerol, 10 mM BME, 5 mM NAD and 5 mM Tyrosine were mixed with 0.2 ul of the MCSG Suite 2 condition #46 (20%w/v PEG 3350, 0.2M Li citrate ) and equilibrated against 1.5 M NaCl solution in 96 Well 3 drop Crystallization Plate (Swissci).
解像度: 2.4→49.12 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.933 / SU B: 18.091 / SU ML: 0.203 / SU R Cruickshank DPI: 0.6184 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.618 / ESU R Free: 0.259 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2155
2131
4.7 %
RANDOM
Rwork
0.167
-
-
-
obs
0.1692
42767
88.15 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK