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- PDB-7m7w: Antibodies to the SARS-CoV-2 receptor-binding domain that maximiz... -

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Basic information

Entry
Database: PDB / ID: 7m7w
TitleAntibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escape
Components
  • (Monoclonal antibody S2H97 Fab ...) x 2
  • (Monoclonal antibody S2X259 Fab ...) x 2
  • Spike protein S1
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / COVID-19 / SARS-CoV-2 / neutralizing monoclonal antibody / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / host cell surface / Virion Assembly and Release / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / host cell surface / Virion Assembly and Release / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / membrane fusion / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / host cell surface receptor binding / symbiont-mediated suppression of host innate immune response / endocytosis involved in viral entry into host cell / receptor ligand activity / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, betacoronavirus / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.65 Å
AuthorsSnell, G. / Czudnochowski, N. / Croll, T.I. / Nix, J.C. / Corti, D. / Cameroni, E. / Pinto, D. / Beltramello, M.
Citation
Journal: Nature / Year: 2021
Title: SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape.
Authors: Tyler N Starr / Nadine Czudnochowski / Zhuoming Liu / Fabrizia Zatta / Young-Jun Park / Amin Addetia / Dora Pinto / Martina Beltramello / Patrick Hernandez / Allison J Greaney / Roberta ...Authors: Tyler N Starr / Nadine Czudnochowski / Zhuoming Liu / Fabrizia Zatta / Young-Jun Park / Amin Addetia / Dora Pinto / Martina Beltramello / Patrick Hernandez / Allison J Greaney / Roberta Marzi / William G Glass / Ivy Zhang / Adam S Dingens / John E Bowen / M Alejandra Tortorici / Alexandra C Walls / Jason A Wojcechowskyj / Anna De Marco / Laura E Rosen / Jiayi Zhou / Martin Montiel-Ruiz / Hannah Kaiser / Josh R Dillen / Heather Tucker / Jessica Bassi / Chiara Silacci-Fregni / Michael P Housley / Julia di Iulio / Gloria Lombardo / Maria Agostini / Nicole Sprugasci / Katja Culap / Stefano Jaconi / Marcel Meury / Exequiel Dellota / Rana Abdelnabi / Shi-Yan Caroline Foo / Elisabetta Cameroni / Spencer Stumpf / Tristan I Croll / Jay C Nix / Colin Havenar-Daughton / Luca Piccoli / Fabio Benigni / Johan Neyts / Amalio Telenti / Florian A Lempp / Matteo S Pizzuto / John D Chodera / Christy M Hebner / Herbert W Virgin / Sean P J Whelan / David Veesler / Davide Corti / Jesse D Bloom / Gyorgy Snell /
Abstract: An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape, have activity against diverse sarbecoviruses, and be highly protective through viral neutralization and effector functions. ...An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape, have activity against diverse sarbecoviruses, and be highly protective through viral neutralization and effector functions. Understanding how these properties relate to each other and vary across epitopes would aid the development of therapeutic antibodies and guide vaccine design. Here we comprehensively characterize escape, breadth and potency across a panel of SARS-CoV-2 antibodies targeting the receptor-binding domain (RBD). Despite a trade-off between in vitro neutralization potency and breadth of sarbecovirus binding, we identify neutralizing antibodies with exceptional sarbecovirus breadth and a corresponding resistance to SARS-CoV-2 escape. One of these antibodies, S2H97, binds with high affinity across all sarbecovirus clades to a cryptic epitope and prophylactically protects hamsters from viral challenge. Antibodies that target the angiotensin-converting enzyme 2 (ACE2) receptor-binding motif (RBM) typically have poor breadth and are readily escaped by mutations despite high neutralization potency. Nevertheless, we also characterize a potent RBM antibody (S2E12) with breadth across sarbecoviruses related to SARS-CoV-2 and a high barrier to viral escape. These data highlight principles underlying variation in escape, breadth and potency among antibodies that target the RBD, and identify epitopes and features to prioritize for therapeutic development against the current and potential future pandemics.
#1: Journal: Biorxiv / Year: 2021
Title: Antibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escape.
Authors: Starr, T.N. / Czudnochowski, N. / Zatta, F. / Park, Y.J. / Liu, Z. / Addetia, A. / Pinto, D. / Beltramello, M. / Hernandez, P. / Greaney, A.J. / Marzi, R. / Glass, W.G. / Zhang, I. / ...Authors: Starr, T.N. / Czudnochowski, N. / Zatta, F. / Park, Y.J. / Liu, Z. / Addetia, A. / Pinto, D. / Beltramello, M. / Hernandez, P. / Greaney, A.J. / Marzi, R. / Glass, W.G. / Zhang, I. / Dingens, A.S. / Bowen, J.E. / Wojcechowskyj, J.A. / De Marco, A. / Rosen, L.E. / Zhou, J. / Montiel-Ruiz, M. / Kaiser, H. / Tucker, H. / Housley, M.P. / di Iulio, J. / Lombardo, G. / Agostini, M. / Sprugasci, N. / Culap, K. / Jaconi, S. / Meury, M. / Dellota, E. / Cameroni, E. / Croll, T.I. / Nix, J.C. / Havenar-Daughton, C. / Telenti, A. / Lempp, F.A. / Pizzuto, M.S. / Chodera, J.D. / Hebner, C.M. / Whelan, S.P.J. / Virgin, H.W. / Veesler, D. / Corti, D. / Bloom, J.D. / Snell, G.
History
DepositionMar 29, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 5, 2021Provider: repository / Type: Initial release
Revision 1.1Jul 21, 2021Group: Database references / Category: citation / citation_author
Revision 1.2Jul 28, 2021Group: Database references / Category: citation / citation_author / Item: _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.3Sep 15, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.name / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / pdbx_initial_refinement_model
Item: _citation.journal_id_ISSN
Revision 1.5Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: Monoclonal antibody S2X259 Fab light chain
A: Monoclonal antibody S2X259 Fab heavy chain
D: Monoclonal antibody S2H97 Fab light chain
C: Monoclonal antibody S2H97 Fab heavy chain
L: Monoclonal antibody S2X259 Fab light chain
H: Monoclonal antibody S2X259 Fab heavy chain
F: Monoclonal antibody S2H97 Fab light chain
E: Monoclonal antibody S2H97 Fab heavy chain
S: Spike protein S1
R: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)238,23512
Polymers237,79310
Non-polymers4422
Water1,71195
1
B: Monoclonal antibody S2X259 Fab light chain
A: Monoclonal antibody S2X259 Fab heavy chain
D: Monoclonal antibody S2H97 Fab light chain
C: Monoclonal antibody S2H97 Fab heavy chain
S: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)119,1186
Polymers118,8965
Non-polymers2211
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
L: Monoclonal antibody S2X259 Fab light chain
H: Monoclonal antibody S2X259 Fab heavy chain
F: Monoclonal antibody S2H97 Fab light chain
E: Monoclonal antibody S2H97 Fab heavy chain
R: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)119,1186
Polymers118,8965
Non-polymers2211
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)86.185, 66.402, 237.659
Angle α, β, γ (deg.)90.000, 94.340, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Antibody , 4 types, 8 molecules BLAHDFCE

#1: Antibody Monoclonal antibody S2X259 Fab light chain


Mass: 22993.350 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody Monoclonal antibody S2X259 Fab heavy chain


Mass: 24351.354 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#3: Antibody Monoclonal antibody S2H97 Fab light chain


Mass: 22935.219 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 suspension / Production host: Homo sapiens (human)
#4: Antibody Monoclonal antibody S2H97 Fab heavy chain


Mass: 24174.143 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 suspension / Production host: Homo sapiens (human)

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Protein / Sugars / Non-polymers , 3 types, 99 molecules SR

#5: Protein Spike protein S1


Mass: 24442.332 Da / Num. of mol.: 2 / Fragment: receptor binding domain (UNP residues 328-531)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#6: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 95 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.95 Å3/Da / Density % sol: 58.36 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop
Details: 30% Precipitant Mix 2 (Molecular Dimensions; ethylene glycol, PEG8000), 0.1 M Buffer System 3, pH 8.5 (Molecular Dimensions; Tris (base)/BICINE), 0.12 M Monosaccharides Mix (Molecular ...Details: 30% Precipitant Mix 2 (Molecular Dimensions; ethylene glycol, PEG8000), 0.1 M Buffer System 3, pH 8.5 (Molecular Dimensions; Tris (base)/BICINE), 0.12 M Monosaccharides Mix (Molecular Dimensions), 0.02 M sodium chloride, 0.01 M MES, pH 6, 3% v/v Jeffamine ED-2003

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.97946 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 21, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
ReflectionResolution: 2.65→63.94 Å / Num. obs: 77307 / % possible obs: 98.6 % / Redundancy: 6.9 % / CC1/2: 0.997 / Rmerge(I) obs: 0.149 / Rpim(I) all: 0.06 / Rrim(I) all: 0.161 / Net I/σ(I): 10.9 / Num. measured all: 533127
Reflection shell

Diffraction-ID: 1 / % possible all: 98.3

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs
2.65-2.76.82.4943064944820.361.0132.6960.8
13.25-63.946.60.025447167810.0110.02849.5

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Processing

Software
NameVersionClassification
REFMAC5.8.0267refinement
Aimlessdata scaling
PDB_EXTRACT3.27data extraction
XDSVERSION Jan 31, 2020 BUILT=20200417data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 7JX3, homology models of S2X259 Fab and S2H97 Fab

7jx3


Resolution: 2.65→50.01 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.908 / SU B: 39.291 / SU ML: 0.347 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.855 / ESU R Free: 0.347 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: U VALUES : WITH TLS ADDED
RfactorNum. reflection% reflectionSelection details
Rfree0.271 3771 4.9 %RANDOM
Rwork0.2212 ---
obs0.2236 73189 97.95 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 176.37 Å2 / Biso mean: 75.747 Å2 / Biso min: 30 Å2
Baniso -1Baniso -2Baniso -3
1-1.49 Å20 Å21.2 Å2
2---1.74 Å20 Å2
3---0.06 Å2
Refinement stepCycle: final / Resolution: 2.65→50.01 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16162 0 28 95 16285
Biso mean--84.22 50.09 -
Num. residues----2136
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0020.01216637
X-RAY DIFFRACTIONr_angle_refined_deg0.8171.64122712
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.6952123
X-RAY DIFFRACTIONr_dihedral_angle_2_deg33.66423.149686
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.912152525
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.6711554
X-RAY DIFFRACTIONr_chiral_restr0.0650.22208
X-RAY DIFFRACTIONr_gen_planes_refined0.0030.0212694
LS refinement shellResolution: 2.65→2.719 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.4 242 -
Rwork0.423 5225 -
all-5467 -
obs--95.41 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.4362-0.0581-0.95090.55940.61665.13780.05510.13710.0315-0.10760.0916-0.1417-0.16020.4186-0.14670.16710.0426-0.06210.2729-0.04970.14949.319417.0385146.8208
20.43760.4625-0.44461.15520.0063.5922-0.09340.1738-0.134-0.08-0.039-0.14590.48860.31420.13250.20230.0459-0.01080.2955-0.04490.06982.53661.7248145.7411
32.8686-1.9383-2.50712.19982.22492.7346-0.2304-0.1176-0.65460.1797-0.11910.28650.38510.02230.34950.29010.01540.01470.34440.05050.277530.8004-33.376878.6366
42.7389-0.4009-1.12031.94891.56663.28620.13440.0587-0.1605-0.3233-0.13750.3682-0.2577-0.07690.00310.22990.01930.03110.28110.05520.240131.3765-20.734367.6491
51.4829-0.5236-0.90360.70820.95935.05520.08640.45620.25-0.2338-0.0354-0.0858-0.2758-0.1143-0.0510.2938-0.0393-0.03120.2310.06550.1454-1.318915.365354.1753
62.7584-0.989-0.98431.34030.22872.9045-0.16160.6163-0.2547-0.2808-0.17250.1410.20670.11270.33410.2657-0.07070.01650.2304-0.0140.0628-4.39110.18853.8165
72.09160.7078-2.61330.5142-1.56896.0484-0.2957-0.7144-0.0280.0457-0.0265-0.17980.53240.45750.32220.61630.0681-0.03010.9662-0.0660.263438.7926.4363-24.0561
81.97730.2327-1.13750.297-0.45794.2028-0.0239-0.86180.31490.35380.0287-0.096-0.71590.2201-0.00480.67910.0802-0.0820.7686-0.16630.209829.0520.1955-22.3707
91.3190.2540.35291.95410.17011.9206-0.06460.6855-0.3327-0.0308-0.12390.16970.27780.07030.18860.74840.02350.12381.5114-0.13330.16217.5759-2.51128.814
102.83310.38960.2142.37580.84751.60440.0351-0.06350.2294-0.23940.1158-0.0736-0.27210.2418-0.15090.2704-0.05290.06310.3387-0.0330.040710.28933.7197102.9978
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A2 - 229
2X-RAY DIFFRACTION2B3 - 219
3X-RAY DIFFRACTION3C1 - 220
4X-RAY DIFFRACTION4D1 - 220
5X-RAY DIFFRACTION5E1 - 223
6X-RAY DIFFRACTION6F1 - 223
7X-RAY DIFFRACTION7H2 - 229
8X-RAY DIFFRACTION8L3 - 219
9X-RAY DIFFRACTION9R332 - 528
10X-RAY DIFFRACTION10S332 - 528

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