+Open data
-Basic information
Entry | Database: PDB / ID: 7m2m | ||||||
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Title | NMR Structure of GCAP5 | ||||||
Components | Guanylate cyclase activator 1A | ||||||
Keywords | METAL BINDING PROTEIN / guanylate cyclase / GCAP5 / EF-hand / Mg2+ / Ca2+ / vision / phototransduction | ||||||
Function / homology | Function and homology information guanylate cyclase activator activity / calcium sensitive guanylate cyclase activator activity / response to stimulus / photoreceptor outer segment / visual perception / ferrous iron binding / calcium ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | Danio rerio (zebrafish) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Authors | Ames, J.B. / Cudia, D.L. | ||||||
Funding support | United States, 1items
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Citation | Journal: Biochemistry / Year: 2021 Title: NMR and EPR-DEER Structure of a Dimeric Guanylate Cyclase Activator Protein-5 from Zebrafish Photoreceptors. Authors: Cudia, D. / Roseman, G.P. / Assafa, T.E. / Shahu, M.K. / Scholten, A. / Menke-Sell, S.K. / Yamada, H. / Koch, K.W. / Milhauser, G. / Ames, J.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7m2m.cif.gz | 508.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7m2m.ent.gz | 436.6 KB | Display | PDB format |
PDBx/mmJSON format | 7m2m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m2/7m2m ftp://data.pdbj.org/pub/pdb/validation_reports/m2/7m2m | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 22489.383 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: The initial MET at residue 1 gets removed during the myristoylation of the protein. The myristoyl group is covalently attached (N-acyl linkage) to the amino group of the GLY at residue 2. Source: (gene. exp.) Danio rerio (zebrafish) / Gene: guca1e, GUCA1E / Production host: Escherichia coli (E. coli) / References: UniProt: Q5MAC8 |
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#2: Chemical | ChemComp-MG / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 Details: structures are based on 660 NOE-derived distances, 128 dihedral angle restraints | |||||||||||||||
NMR representative | Selection criteria: medoid | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 9 |