[English] 日本語
![](img/lk-miru.gif)
- PDB-7m1u: Crystal structure of an archaeal CNNM, MtCorB, R235L mutant with ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 7m1u | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of an archaeal CNNM, MtCorB, R235L mutant with C-terminal deletion | |||||||||
![]() | Hemolysin, contains CBS domains | |||||||||
![]() | MEMBRANE PROTEIN / CorB / CNNM / DUF21 / magnesium transporter | |||||||||
Function / homology | ![]() methionine biosynthetic process / flavin adenine dinucleotide binding / ATP binding / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chen, Y.S. / Kozlov, G. / Gehring, K. | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Crystal structure of an archaeal CorB magnesium transporter. Authors: Chen, Y.S. / Kozlov, G. / Moeller, B.E. / Rohaim, A. / Fakih, R. / Roux, B. / Burke, J.E. / Gehring, K. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 382.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 264.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 431.2 KB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 28.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7m1tC ![]() 7msuC ![]() 7lj6 ![]() 7lj7 C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 36591.164 Da / Num. of mol.: 2 / Mutation: R235L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 5.08 Å3/Da / Density % sol: 75.79 % |
---|---|
Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 0.1 M sodium citrate, pH 5.5; 50 mM Li2SO4; 11% PEG4000; 10 mM MgSO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 14, 2021 |
Radiation | Monochromator: KOHZU double crystal monochromator (DCM) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.52154 Å / Relative weight: 1 |
Reflection | Resolution: 3.8→50 Å / Num. obs: 15203 / % possible obs: 91.7 % / Redundancy: 6 % / CC1/2: 0.965 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 3.8→3.94 Å / Redundancy: 4.5 % / Mean I/σ(I) obs: 0.8 / Num. unique obs: 152 / CC1/2: 0.51 / % possible all: 78.7 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 7LJ6, 7LJ7 Resolution: 3.8→46.27 Å / SU ML: 0.6517 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 33.632 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| ||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 95.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.8→46.27 Å
| ||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -12.7023134885 Å / Origin y: -30.0606052659 Å / Origin z: 23.9817900124 Å
| ||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |