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- PDB-7lzp: LC/A-JPU-B9-JPU-A11-JPU-G11 -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7lzp
TitleLC/A-JPU-B9-JPU-A11-JPU-G11
Components
  • Botulinum neurotoxin A light chain
  • JPU-A11
  • JPU-B9
  • JPU-G11
KeywordsANTITOXIN / Single domain antibody / VHH / protease inhibitor
Function / homology
Function and homology information


symbiont-mediated suppression of host exocytosis / ganglioside GT1b binding / Toxicity of botulinum toxin type A (botA) / bontoxilysin / host cell presynaptic membrane / host cell cytoplasmic vesicle / host cell cytosol / protein transmembrane transporter activity / metalloendopeptidase activity / toxin activity ...symbiont-mediated suppression of host exocytosis / ganglioside GT1b binding / Toxicity of botulinum toxin type A (botA) / bontoxilysin / host cell presynaptic membrane / host cell cytoplasmic vesicle / host cell cytosol / protein transmembrane transporter activity / metalloendopeptidase activity / toxin activity / host cell plasma membrane / proteolysis / extracellular region / zinc ion binding / membrane
Similarity search - Function
Clostridium neurotoxin, translocation / Clostridium neurotoxin, Translocation domain / Clostridium neurotoxin, translocation domain / Clostridium neurotoxin, receptor-binding C-terminal / Clostridium neurotoxin, C-terminal receptor binding / Clostridial neurotoxin zinc protease / Botulinum/Tetanus toxin, catalytic chain / Clostridium neurotoxin, receptor binding N-terminal / Clostridium neurotoxin, N-terminal receptor binding / Kunitz inhibitor STI-like superfamily ...Clostridium neurotoxin, translocation / Clostridium neurotoxin, Translocation domain / Clostridium neurotoxin, translocation domain / Clostridium neurotoxin, receptor-binding C-terminal / Clostridium neurotoxin, C-terminal receptor binding / Clostridial neurotoxin zinc protease / Botulinum/Tetanus toxin, catalytic chain / Clostridium neurotoxin, receptor binding N-terminal / Clostridium neurotoxin, N-terminal receptor binding / Kunitz inhibitor STI-like superfamily / Neutral zinc metallopeptidases, zinc-binding region signature. / Concanavalin A-like lectin/glucanase domain superfamily / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Botulinum neurotoxin type A
Similarity search - Component
Biological speciesClostridium botulinum (bacteria)
Vicugna pacos (alpaca)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.86 Å
AuthorsLam, K. / Jin, R.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: Plos Pathog. / Year: 2022
Title: Probing the structure and function of the protease domain of botulinum neurotoxins using single-domain antibodies.
Authors: Lam, K.H. / Tremblay, J.M. / Perry, K. / Ichtchenko, K. / Shoemaker, C.B. / Jin, R.
History
DepositionMar 10, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 22, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 19, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.year
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.3Oct 9, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Botulinum neurotoxin A light chain
C: JPU-G11
D: Botulinum neurotoxin A light chain
E: JPU-A11
G: JPU-B9
F: JPU-G11
B: JPU-A11
H: JPU-B9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)183,36812
Polymers183,1078
Non-polymers2624
Water362
1
A: Botulinum neurotoxin A light chain
C: JPU-G11
G: JPU-B9
B: JPU-A11
hetero molecules


Theoretical massNumber of molelcules
Total (without water)91,6846
Polymers91,5534
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: Botulinum neurotoxin A light chain
E: JPU-A11
F: JPU-G11
H: JPU-B9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)91,6846
Polymers91,5534
Non-polymers1312
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)132.604, 132.604, 413.030
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number179
Space group name H-MP6522
Space group name HallP652(x,y,z+1/12)
Symmetry operation#1: x,y,z
#2: x-y,x,z+5/6
#3: y,-x+y,z+1/6
#4: -y,x-y,z+2/3
#5: -x+y,-x,z+1/3
#6: x-y,-y,-z
#7: -x,-x+y,-z+1/3
#8: -x,-y,z+1/2
#9: y,x,-z+2/3
#10: -y,-x,-z+1/6
#11: -x+y,y,-z+1/2
#12: x,x-y,-z+5/6
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
12
22
13
23
14
24

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111PROPROASNASN(chain 'A' and (resid 2 through 64 or resid 66...AA2 - 269 - 33
121METMETPROPRO(chain 'A' and (resid 2 through 64 or resid 66...AA30 - 6337 - 70
131LYSLYSPHEPHE(chain 'A' and (resid 2 through 64 or resid 66...AA66 - 19473 - 201
141GLUGLUGLUGLU(chain 'A' and (resid 2 through 64 or resid 66...AA197 - 201204 - 208
151PHEPHEASNASN(chain 'A' and (resid 2 through 64 or resid 66...AA213 - 246220 - 253
161LEULEUASNASN(chain 'A' and (resid 2 through 64 or resid 66...AA256 - 410263 - 417
171ASNASNLYSLYS(chain 'A' and (resid 2 through 64 or resid 66...AA412 - 438419 - 445
281PROPROASNASN(chain 'D' and (resid 2 through 6 or (resid 7...DC2 - 269 - 33
291METMETPROPRO(chain 'D' and (resid 2 through 6 or (resid 7...DC30 - 6337 - 70
2101LYSLYSPHEPHE(chain 'D' and (resid 2 through 6 or (resid 7...DC66 - 19473 - 201
2111GLUGLUGLUGLU(chain 'D' and (resid 2 through 6 or (resid 7...DC197 - 201204 - 208
2121PHEPHEASNASN(chain 'D' and (resid 2 through 6 or (resid 7...DC213 - 246220 - 253
2131LEULEUASNASN(chain 'D' and (resid 2 through 6 or (resid 7...DC256 - 410263 - 417
2141ASNASNLYSLYS(chain 'D' and (resid 2 through 6 or (resid 7...DC412 - 438419 - 445
1152GLNGLNTHRTHR(chain 'B' and ((resid 1 and (name N or name...BG1 - 256 - 30
1162THRTHRVALVAL(chain 'B' and ((resid 1 and (name N or name...BG28 - 12833 - 133
2172GLNGLNTHRTHR(chain 'E' and (resid 1 through 12 or (resid 13...ED1 - 256 - 30
2182THRTHRVALVAL(chain 'E' and (resid 1 through 12 or (resid 13...ED28 - 12833 - 133
1193GLNGLNVALVAL(chain 'C' and (resid 3 through 10 or (resid 11...CB3 - 128 - 17
1203PROPROVALVAL(chain 'C' and (resid 3 through 10 or (resid 11...CB14 - 11519 - 120
2213GLNGLNVALVALchain 'F'FF3 - 128 - 17
2223PROPROVALVALchain 'F'FF14 - 11519 - 120
1234VALVALGLNGLN(chain 'G' and (resid 2 through 38 or (resid 39...GE2 - 397 - 44
1244GLNGLNSERSER(chain 'G' and (resid 2 through 38 or (resid 39...GE44 - 11049 - 115
2254VALVALGLNGLNchain 'H'HH2 - 397 - 44
2264GLNGLNSERSERchain 'H'HH44 - 11049 - 115

NCS ensembles :
ID
1
2
3
4

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Components

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Protein , 1 types, 2 molecules AD

#1: Protein Botulinum neurotoxin A light chain / LC


Mass: 50894.594 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridium botulinum (bacteria) / Gene: botA, atx, bonT / Production host: Escherichia coli (E. coli) / References: UniProt: P0DPI0, bontoxilysin

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Antibody , 3 types, 6 molecules CFEBGH

#2: Antibody JPU-G11


Mass: 13515.010 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
#3: Antibody JPU-A11


Mass: 14714.107 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
#4: Antibody JPU-B9


Mass: 12429.715 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)

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Non-polymers , 2 types, 6 molecules

#5: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.86 Å3/Da / Density % sol: 57.03 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop
Details: 0.1 M sodium cacodylate pH 6.5, 15 % ethylene glycol, 25 % PEG 4000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 10, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.86→137.68 Å / Num. obs: 50539 / % possible obs: 99.6 % / Redundancy: 9.7 % / Biso Wilson estimate: 79.1 Å2 / CC1/2: 0.998 / Rpim(I) all: 0.088 / Net I/σ(I): 12.2
Reflection shellResolution: 2.86→2.96 Å / Num. unique obs: 4409 / CC1/2: 0.405 / Rpim(I) all: 0.893

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
PHASERphasing
MOSFLMdata reduction
Aimlessdata scaling
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3V0A

3v0a


Resolution: 2.86→67.06 Å / SU ML: 0.4983 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.0495 / Stereochemistry target values: CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2727 2533 5.03 %
Rwork0.2346 47847 -
obs0.2365 50380 99.69 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 81.97 Å2
Refinement stepCycle: LAST / Resolution: 2.86→67.06 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12106 0 4 2 12112
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002812418
X-RAY DIFFRACTIONf_angle_d0.583916870
X-RAY DIFFRACTIONf_chiral_restr0.04451867
X-RAY DIFFRACTIONf_plane_restr0.00382163
X-RAY DIFFRACTIONf_dihedral_angle_d12.62864412
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.86-2.920.39211490.34282465X-RAY DIFFRACTION95.23
2.92-2.980.33471330.31762620X-RAY DIFFRACTION100
2.98-3.040.36291390.34772596X-RAY DIFFRACTION100
3.04-3.110.36031410.33972616X-RAY DIFFRACTION100
3.11-3.190.3541460.32392618X-RAY DIFFRACTION99.89
3.19-3.280.38181350.30522609X-RAY DIFFRACTION100
3.28-3.370.34121350.28532621X-RAY DIFFRACTION99.96
3.37-3.480.31871430.29112630X-RAY DIFFRACTION99.93
3.48-3.610.34531440.28852616X-RAY DIFFRACTION100
3.61-3.750.31571200.26832647X-RAY DIFFRACTION100
3.75-3.920.26911380.23982660X-RAY DIFFRACTION99.93
3.92-4.130.27211250.22232684X-RAY DIFFRACTION100
4.13-4.390.24381430.19862641X-RAY DIFFRACTION99.96
4.39-4.730.21281400.17932683X-RAY DIFFRACTION99.93
4.73-5.20.23891420.18192689X-RAY DIFFRACTION100
5.2-5.960.271400.20492734X-RAY DIFFRACTION99.97
5.96-7.50.28051550.21362755X-RAY DIFFRACTION99.86
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.312835425630.0368974995328-0.2040832920241.10537988716-1.421219359752.042674158680.0712733126816-0.257632901123-0.04901906942340.0679956204701-0.172519226942-0.5908036344260.1800198409660.09391909038860.0001843339200680.2767552216770.0635571094444-0.04797112366770.9160281252580.003930452318490.55839411557170.8500949705-79.1993958082-1.45279489238
22.52500801250.599356915173-0.3664221152220.262634995728-0.207421285881.505284921670.252941792826-0.3480074070020.055741965680.164148584878-0.100071323047-0.0222937404645-0.1276319374810.05018772732021.83325043154E-80.4332815096310.0529560449163-0.0542164067910.809375143723-0.125847997630.54485277568761.4521672118-76.05970498870.265270254282
30.2564856164650.01713671435850.1626135744021.61994639285-0.390577056830.3173024313430.188484041008-0.3478768469880.366642604082-0.181321516645-0.0447299010947-0.2452895801150.01523072644950.4402012175970.0068545431520.4160173946920.01681597696870.01066625913320.905070170827-0.1399621650440.58078886882262.3389137258-69.1244908239-2.41342121025
43.0579164010.0623094579951-0.3949524566741.305289930350.6375330299670.7194930770280.603238818529-0.881400304805-1.059777190.0341021375591-0.4702781090950.4923966853080.474192714272-0.07610272679490.1336752634260.445011131542-0.0614220657372-0.1361035590050.939599177841-0.1963263743620.55480000219749.8231452956-85.23824147978.88233131546
52.019301771460.0997966152299-0.2920557139592.41525752231-0.8685940136252.515978929230.1819605021610.1510251263220.227474872259-0.255193873331-0.01010114975830.08673159524040.00775533724994-0.00406449255001-2.07528946375E-70.3532254024770.0879330597083-0.1181507760580.67978950608-0.150572563460.54919409847547.1311315647-71.6266933383-11.3476229261
62.009471262920.5809381826780.1858902714511.18676530681-1.022988795551.17911070709-0.0573061463276-0.353452705407-0.5103066697660.09454256794370.02993431484880.4215768909760.2384444702630.0966715260413-0.0001565595680380.4694328493940.0497380127437-0.07048091847830.83589555161-0.01312380671980.57279988807447.6552978354-88.271604093110.2236570892
70.253666064234-0.25749632843-0.05898867846020.277953029505-0.0390261768690.262812896144-0.6136539293460.244733711701-0.1187007645730.1745078049080.313798991994-0.2345501821940.6908323569820.299625325761-0.007378523573621.15058985393-0.337906194399-0.1837692182950.966049628526-0.2114562779470.6835541404543.3230073692-105.940851557-27.0217922096
80.5977568765360.2542716661190.02248968051790.9349747694640.3163995412630.1166944277860.0973925105556-0.0518960453078-0.880478598993-0.0516250256345-0.4549571279440.577036064589-0.167753328278-0.0902370314906-0.05586152091040.988534809129-0.466562400618-0.2407071591551.00693010188-0.02377255687660.71203257276837.6097813728-103.690231404-26.7270896252
90.796410593690.0307462777245-1.222697663940.8595639697380.6895974622892.52404882957-0.0207729295246-0.4171782215160.1943425310470.403772632891-0.4300242820860.4466996465181.04977291684-0.0382853697093-0.9561774303340.631449369111-0.0766434886331-0.07576417273781.19327554561-0.3726112781540.44006530534542.8820931449-90.8750272041-15.1469012899
100.2353365539910.250949203623-0.01559797051570.2455960941260.006851148711330.002832185706820.08588297383890.5283838348070.425868400189-0.66101912667-0.370977689238-0.1268654405640.19465700648-0.268880593601-2.6165565903E-60.8187289836150.0359737000326-0.08324159982420.849254181202-0.07465027736960.67896809753847.0369670101-96.1854606893-32.9346616975
110.0203201594745-0.03608403265860.01366712166650.0530109718828-0.009537025831920.0277905286248-0.348419090844-0.259394603414-0.03403308814840.2282065305890.535460519937-0.0631463818305-0.148079703481-0.0239949919278-3.10155271472E-50.807100398605-0.12738098192-0.1565592363051.08676803821-0.03018332598260.56712441678536.4778707409-87.810121372-29.4198830546
120.03537718116560.0326639319030.09548973617370.207157106990.0306986791170.1171271977160.210648421875-0.1998988092380.0210200254304-0.961803878633-0.06644452123330.1204926924380.253868057541-0.228172014687-6.77600564689E-70.9265686465230.00813703515103-0.146088151630.911138821935-0.1033735689370.60070292344136.2230051381-95.3597064522-29.8399033451
130.1700534002150.2261318069780.05624173004720.1714636879180.03843133818510.0167262783264-0.4372610652570.3949859857950.0775892430481-0.791465739695-0.1561999901230.423897226386-0.00212628072708-0.156760632166-5.49865691614E-80.738420917459-0.118065211771-0.07132467358060.935579100684-0.1756192262480.58916920836541.7603272076-100.268464345-32.1882691413
14-0.0143208414184-0.02225082955740.1973076432290.0688606846557-0.1989663756790.6906220960860.0888266799993-0.509758622072-0.304741696764-0.6891384602920.460702724953-0.09192656584010.9244962064850.260400182990.03811459411040.7190067346050.0430978150652-0.0650922504280.848765364468-0.08953412008340.59624824148648.7819757063-96.6450438528-26.2004795231
151.23321408477-0.07084302392990.3519067225630.1262020273520.2331103986922.16772802993-0.316703005238-0.201832169869-0.07606149190070.2711199563970.3831176608770.06494377140120.0364500130691-0.2377657739920.000156266714380.3747720671660.01191153334480.006910531392370.841213546751-0.1481023972210.59595580899231.5557182934-37.33325660957.06872844069
161.40685241581-0.1453648793850.4554132600240.67840272030.8258598902031.763811008920.130453719938-0.757694068439-0.3860639589380.384205914345-0.00530840344523-0.3164657249590.188360312640.0189602389412-3.85701131762E-70.4439909073190.0498709895514-5.94464923504E-50.817654252519-0.09836051430750.61542931511640.1504102705-40.313928368418.7743131066
170.707397459153-0.2148785246150.2548496239950.24455676858-0.3455808042941.011870684790.01353126994630.272694611178-0.238397013616-0.0636037892339-0.004703479767740.2603047297130.1858976275280.1012108896372.15353962885E-70.369078201933-0.09336062119540.01607123928440.774644362258-0.2556946048260.51557006446942.0984552732-40.7943146597-7.31437954821
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640.3413308458680.6739092993620.2403631345971.321705591130.463060185670.513423172736-0.183763337406-0.155349153371-0.2836330582620.330350170349-0.160901404306-0.1035629574390.02399072818630.0960848069446-0.003302517377611.641688611891.12080870318-0.307489177562.60236407459-0.492039068821.847531957393.2954224473-53.364001390325.5684137884
651.30939837475-1.032081980440.03398221574991.24329616406-0.1723298523480.397044862335-0.19970144153-0.5219779375840.5289138449880.0283958440867-0.3168509353090.2245420060120.09315282950630.581694483037-0.9147230202530.08578457689210.415795471775-0.2129757330242.28459549711-0.9853914065311.4172843976681.9083936858-44.647200557712.553583205
660.4800713597780.1528087658620.1304249775290.05500060234680.05313432512640.0536867424312-0.2000383687260.05360357853530.1728267801330.0904509331249-0.02824587094950.3120143644870.126367074564-0.0148790255035-0.1100804010311.236657159580.711941812295-0.2790701314342.12067559677-0.7473655560981.4462879131596.2241014614-50.052533362517.887840696
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 53 )
2X-RAY DIFFRACTION2chain 'A' and (resid 54 through 138 )
3X-RAY DIFFRACTION3chain 'A' and (resid 139 through 182 )
4X-RAY DIFFRACTION4chain 'A' and (resid 183 through 216 )
5X-RAY DIFFRACTION5chain 'A' and (resid 217 through 358 )
6X-RAY DIFFRACTION6chain 'A' and (resid 359 through 438 )
7X-RAY DIFFRACTION7chain 'C' and (resid 1 through 16 )
8X-RAY DIFFRACTION8chain 'C' and (resid 17 through 23 )
9X-RAY DIFFRACTION9chain 'C' and (resid 24 through 31 )
10X-RAY DIFFRACTION10chain 'C' and (resid 32 through 51 )
11X-RAY DIFFRACTION11chain 'C' and (resid 52 through 59 )
12X-RAY DIFFRACTION12chain 'C' and (resid 60 through 76 )
13X-RAY DIFFRACTION13chain 'C' and (resid 77 through 99 )
14X-RAY DIFFRACTION14chain 'C' and (resid 100 through 116 )
15X-RAY DIFFRACTION15chain 'D' and (resid 2 through 53 )
16X-RAY DIFFRACTION16chain 'D' and (resid 54 through 100 )
17X-RAY DIFFRACTION17chain 'D' and (resid 101 through 138 )
18X-RAY DIFFRACTION18chain 'D' and (resid 139 through 182 )
19X-RAY DIFFRACTION19chain 'D' and (resid 183 through 256 )
20X-RAY DIFFRACTION20chain 'D' and (resid 257 through 298 )
21X-RAY DIFFRACTION21chain 'D' and (resid 299 through 390 )
22X-RAY DIFFRACTION22chain 'D' and (resid 391 through 438 )
23X-RAY DIFFRACTION23chain 'E' and (resid 1 through 39 )
24X-RAY DIFFRACTION24chain 'E' and (resid 40 through 51 )
25X-RAY DIFFRACTION25chain 'E' and (resid 52 through 61 )
26X-RAY DIFFRACTION26chain 'E' and (resid 62 through 73 )
27X-RAY DIFFRACTION27chain 'E' and (resid 74 through 91 )
28X-RAY DIFFRACTION28chain 'E' and (resid 92 through 113 )
29X-RAY DIFFRACTION29chain 'E' and (resid 114 through 130 )
30X-RAY DIFFRACTION30chain 'G' and (resid 1 through 7 )
31X-RAY DIFFRACTION31chain 'G' and (resid 8 through 24 )
32X-RAY DIFFRACTION32chain 'G' and (resid 25 through 32 )
33X-RAY DIFFRACTION33chain 'G' and (resid 33 through 52 )
34X-RAY DIFFRACTION34chain 'G' and (resid 53 through 66 )
35X-RAY DIFFRACTION35chain 'G' and (resid 67 through 76 )
36X-RAY DIFFRACTION36chain 'G' and (resid 77 through 96 )
37X-RAY DIFFRACTION37chain 'G' and (resid 97 through 110 )
38X-RAY DIFFRACTION38chain 'F' and (resid 3 through 16 )
39X-RAY DIFFRACTION39chain 'F' and (resid 17 through 23 )
40X-RAY DIFFRACTION40chain 'F' and (resid 24 through 31 )
41X-RAY DIFFRACTION41chain 'F' and (resid 32 through 39 )
42X-RAY DIFFRACTION42chain 'F' and (resid 40 through 51 )
43X-RAY DIFFRACTION43chain 'F' and (resid 52 through 59 )
44X-RAY DIFFRACTION44chain 'F' and (resid 60 through 66 )
45X-RAY DIFFRACTION45chain 'F' and (resid 67 through 76 )
46X-RAY DIFFRACTION46chain 'F' and (resid 77 through 83 )
47X-RAY DIFFRACTION47chain 'F' and (resid 84 through 90 )
48X-RAY DIFFRACTION48chain 'F' and (resid 91 through 99 )
49X-RAY DIFFRACTION49chain 'F' and (resid 100 through 110 )
50X-RAY DIFFRACTION50chain 'F' and (resid 111 through 115 )
51X-RAY DIFFRACTION51chain 'B' and (resid 0 through 7 )
52X-RAY DIFFRACTION52chain 'B' and (resid 8 through 51 )
53X-RAY DIFFRACTION53chain 'B' and (resid 52 through 60 )
54X-RAY DIFFRACTION54chain 'B' and (resid 61 through 73 )
55X-RAY DIFFRACTION55chain 'B' and (resid 74 through 83 )
56X-RAY DIFFRACTION56chain 'B' and (resid 84 through 110 )
57X-RAY DIFFRACTION57chain 'B' and (resid 111 through 128 )
58X-RAY DIFFRACTION58chain 'H' and (resid 2 through 17 )
59X-RAY DIFFRACTION59chain 'H' and (resid 18 through 39 )
60X-RAY DIFFRACTION60chain 'H' and (resid 40 through 52 )
61X-RAY DIFFRACTION61chain 'H' and (resid 53 through 59 )
62X-RAY DIFFRACTION62chain 'H' and (resid 60 through 66 )
63X-RAY DIFFRACTION63chain 'H' and (resid 67 through 82 )
64X-RAY DIFFRACTION64chain 'H' and (resid 83 through 90 )
65X-RAY DIFFRACTION65chain 'H' and (resid 91 through 104 )
66X-RAY DIFFRACTION66chain 'H' and (resid 105 through 110 )

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