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- PDB-7ldw: Zoogloea ramigera biosynthetic thiolase Q183Y/Y218E/delH221/S227K... -

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Basic information

Entry
Database: PDB / ID: 7ldw
TitleZoogloea ramigera biosynthetic thiolase Q183Y/Y218E/delH221/S227K/G248T mutant
ComponentsAcetyl-CoA acetyltransferase
KeywordsTRANSFERASE / acetyl-CoA C-acetyltransferase / acetoacetyl-CoA thiolase / type II thiolase / biosynthetic thiolase / potassium activation
Function / homology
Function and homology information


poly-hydroxybutyrate biosynthetic process / acetyl-CoA C-acetyltransferase / acetyl-CoA C-acetyltransferase activity / cytoplasm
Similarity search - Function
Thiolase, active site / Thiolases active site. / Thiolase, conserved site / Thiolases signature 2. / Thiolase, acyl-enzyme intermediate active site / Thiolases acyl-enzyme intermediate signature. / Thiolase, C-terminal / Thiolase, C-terminal domain / Thiolase / Thiolase, N-terminal ...Thiolase, active site / Thiolases active site. / Thiolase, conserved site / Thiolases signature 2. / Thiolase, acyl-enzyme intermediate active site / Thiolases acyl-enzyme intermediate signature. / Thiolase, C-terminal / Thiolase, C-terminal domain / Thiolase / Thiolase, N-terminal / Thiolase, N-terminal domain / Thiolase-like
Similarity search - Domain/homology
COENZYME A / AMMONIUM ION / Acetyl-CoA acetyltransferase
Similarity search - Component
Biological speciesZoogloea ramigera (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.5 Å
AuthorsMarshall, A.C. / Bruning, J.B.
CitationJournal: Biochem.J. / Year: 2021
Title: Engineering potassium activation into biosynthetic thiolase.
Authors: Marshall, A.C. / Bruning, J.B.
History
DepositionJan 14, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 1, 2021Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.2Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Acetyl-CoA acetyltransferase
B: Acetyl-CoA acetyltransferase
C: Acetyl-CoA acetyltransferase
D: Acetyl-CoA acetyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)170,14622
Polymers165,9694
Non-polymers4,17718
Water8,899494
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area20200 Å2
ΔGint-157 kcal/mol
Surface area48970 Å2
MethodPISA
Unit cell
Length a, b, c (Å)84.595, 79.177, 152.648
Angle α, β, γ (deg.)90.000, 93.720, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 3 through 39 or (resid 40...
21(chain B and (resid 3 through 39 or (resid 40...
31(chain C and (resid 3 through 166 or (resid 167...
41(chain D and (resid 3 through 39 or (resid 40...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11PROPROLEULEU(chain A and (resid 3 through 39 or (resid 40...AA3 - 3910 - 46
12GLUGLUGLUGLU(chain A and (resid 3 through 39 or (resid 40...AA4047
13PROPROLEULEU(chain A and (resid 3 through 39 or (resid 40...AA3 - 39110 - 398
14PROPROLEULEU(chain A and (resid 3 through 39 or (resid 40...AA3 - 39110 - 398
15PROPROLEULEU(chain A and (resid 3 through 39 or (resid 40...AA3 - 39110 - 398
16PROPROLEULEU(chain A and (resid 3 through 39 or (resid 40...AA3 - 39110 - 398
21PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 3910 - 46
22GLUGLUGLUGLU(chain B and (resid 3 through 39 or (resid 40...BB4047
23PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 39110 - 398
24PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 39110 - 398
25PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 39110 - 398
26PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 39110 - 398
27PROPROLEULEU(chain B and (resid 3 through 39 or (resid 40...BB3 - 39110 - 398
31PROPROLYSLYS(chain C and (resid 3 through 166 or (resid 167...CC3 - 16610 - 173
32GLNGLNGLNGLN(chain C and (resid 3 through 166 or (resid 167...CC167174
33PROPROLEULEU(chain C and (resid 3 through 166 or (resid 167...CC3 - 39110 - 398
34PROPROLEULEU(chain C and (resid 3 through 166 or (resid 167...CC3 - 39110 - 398
35PROPROLEULEU(chain C and (resid 3 through 166 or (resid 167...CC3 - 39110 - 398
36PROPROLEULEU(chain C and (resid 3 through 166 or (resid 167...CC3 - 39110 - 398
37PROPROLEULEU(chain C and (resid 3 through 166 or (resid 167...CC3 - 39110 - 398
41PROPROLEULEU(chain D and (resid 3 through 39 or (resid 40...DD3 - 3910 - 46
42GLUGLUGLUGLU(chain D and (resid 3 through 39 or (resid 40...DD4047
43PROPROLEULEU(chain D and (resid 3 through 39 or (resid 40...DD3 - 39110 - 398
44PROPROLEULEU(chain D and (resid 3 through 39 or (resid 40...DD3 - 39110 - 398
45PROPROLEULEU(chain D and (resid 3 through 39 or (resid 40...DD3 - 39110 - 398
46PROPROLEULEU(chain D and (resid 3 through 39 or (resid 40...DD3 - 39110 - 398

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Acetyl-CoA acetyltransferase / Acetoacetyl-CoA thiolase / Beta-ketothiolase


Mass: 41492.312 Da / Num. of mol.: 4 / Mutation: Q183Y, Y218E, S227K, G248T
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Zoogloea ramigera (bacteria) / Gene: phaA, phbA / Plasmid: pET11a / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P07097, acetyl-CoA C-acetyltransferase

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Non-polymers , 5 types, 512 molecules

#2: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: SO4
#3: Chemical
ChemComp-COA / COENZYME A


Mass: 767.534 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C21H36N7O16P3S / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical ChemComp-NH4 / AMMONIUM ION


Mass: 18.038 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: H4N / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 494 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.07 Å3/Da / Density % sol: 59.98 % / Mosaicity: 0.14 °
Crystal growTemperature: 289 K / Method: vapor diffusion / pH: 5.5
Details: 1.32 M (NH4)2SO4, 0.88 M Li2SO4, 0.1 M sodium citrate pH 5.5, 1 mM DTT, 1 mM EDTA, 1 mM NaN3

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95374 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 14, 2018
RadiationMonochromator: Silicon Double Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95374 Å / Relative weight: 1
ReflectionResolution: 2.5→34.68 Å / Num. obs: 69939 / % possible obs: 99.9 % / Redundancy: 7 % / CC1/2: 0.984 / Rmerge(I) obs: 0.418 / Rpim(I) all: 0.17 / Rrim(I) all: 0.452 / Net I/σ(I): 4.8 / Num. measured all: 488279 / Scaling rejects: 16
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.5-2.566.93.9413084545010.2241.6234.2680.7100
11.99-34.686.50.06842296530.9970.0280.07416.995.5

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation7.87 Å34.68 Å
Translation7.87 Å34.68 Å

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Processing

Software
NameVersionClassification
XDSdata reduction
Aimless0.7.2data scaling
PHASER2.8.2phasing
PHENIX1.15_3459refinement
PDB_EXTRACT3.27data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1qfl

1qfl


Resolution: 2.5→34.68 Å / SU ML: 0.45 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 28.77 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2474 3462 4.96 %
Rwork0.1994 66397 -
obs0.2017 69859 99.86 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 129.05 Å2 / Biso mean: 56.5668 Å2 / Biso min: 11.81 Å2
Refinement stepCycle: final / Resolution: 2.5→34.68 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11129 0 251 494 11874
Biso mean--72.61 49.99 -
Num. residues----1555
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00211537
X-RAY DIFFRACTIONf_angle_d0.60415634
X-RAY DIFFRACTIONf_dihedral_angle_d14.8954074
X-RAY DIFFRACTIONf_chiral_restr0.0441760
X-RAY DIFFRACTIONf_plane_restr0.0032034
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6849X-RAY DIFFRACTION9.371TORSIONAL
12B6849X-RAY DIFFRACTION9.371TORSIONAL
13C6849X-RAY DIFFRACTION9.371TORSIONAL
14D6849X-RAY DIFFRACTION9.371TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.5-2.53420.40191510.3816258199
2.5342-2.57040.37631480.36022655100
2.5704-2.60880.38371510.35062593100
2.6088-2.64950.36551380.32922661100
2.6495-2.6930.38241560.32432597100
2.693-2.73940.35321450.31472647100
2.7394-2.78920.32831370.2952617100
2.7892-2.84280.34961250.29192674100
2.8428-2.90080.32581580.28192617100
2.9008-2.96380.34141290.26972665100
2.9638-3.03270.30291400.24952647100
3.0327-3.10850.27571220.2362641100
3.1085-3.19250.30561190.24162682100
3.1925-3.28640.2991510.21322638100
3.2864-3.39240.27571070.20182710100
3.3924-3.51350.25931470.18772633100
3.5135-3.65410.27471360.1782640100
3.6541-3.82020.23971220.1722691100
3.8202-4.02130.16381220.14972652100
4.0213-4.27280.18561120.13872712100
4.2728-4.6020.1641410.12662661100
4.602-5.06390.15661260.13692690100
5.0639-5.79370.21581590.17232673100
5.7937-7.28830.22871560.17762688100
7.2883-34.680.18871640.16522732100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.4049-0.34520.28221.63480.30795.682-0.305-0.18060.07840.2459-0.0292-0.03490.3319-0.52830.39420.19690.00310.13160.2887-0.01060.457837.1684-6.87029.1271
26.8631-4.2457-2.42285.5970.23127.19330.0537-0.15510.0774-0.06670.0797-0.6158-0.40850.9799-0.10810.2562-0.07770.04010.2133-0.07250.548440.38882.46343.1529
33.48772.4453-2.23585.1671-0.61652.43020.24740.02680.11060.3619-0.0315-0.2609-0.22950.1823-0.1790.25630.02390.07420.3602-0.00810.733130.99026.07468.9796
41.76650.5523-2.98340.21350.29438.4154-0.01080.0801-0.01510.1546-0.0227-0.0499-0.1981-0.39680.01710.30790.06120.03920.2795-0.01730.809123.0861-1.812112.5177
58.22130.1269-0.20618.86924.65563.6692-0.50310.3725-0.30020.03510.36051.3081-0.0058-0.12920.12550.3927-0.02030.09060.36490.07150.803317.1329-20.919815.6988
62.19668.0264-7.31429.5738-4.30149.6223-0.0449-0.2692-1.07860.05980.2835-0.4770.3186-0.04-0.28330.32240.09340.02280.3270.11940.623836.7497-28.903311.9366
78.5278-3.965-5.84282.88998.36629.91190.3623-0.30120.32671.32991.1585-1.7730.74810.8005-1.39520.3990.06290.03670.43340.07420.872247.0255-8.147612.604
89.40016.9293-1.36559.8439-3.09093.83860.0415-0.20120.52960.3655-0.0302-0.0911-0.02090.29810.00420.38740.08710.01230.4201-0.04340.90536.9436-26.087120.1607
91.88050.0808-0.06713.019-0.18061.70020.06130.0331-0.0103-0.01960.07980.0569-0.0525-0.0173-0.11260.12770.06030.06820.2214-0.04580.841130.8238-11.61291.308
104.41881.8351-4.25580.8336-2.48959.0861-0.2970.199-0.79810.0343-0.09650.21190.5209-0.23210.38850.44540.0072-0.04370.2212-0.00770.843923.9128-20.9121-2.9164
114.1092.28750.3851.19710.56518.0698-0.1854-0.0473-0.0116-0.0472-0.12890.15910.1217-0.46520.38440.32150.04550.03660.27680.01140.937930.1454-25.2443.9955
126.2921-0.682-2.69871.89651.9354.659-0.1302-0.1478-0.4068-0.11470.0875-0.2830.07170.34010.01440.31710.01190.06740.21640.04670.72232.7407-15.1681-1.639
130.7536-0.1364-0.26961.22770.83084.5354-0.0247-0.1045-0.11120.1470.02920.14490.4008-0.07230.00040.2560.00170.02670.29840.04170.69026.32421.36117.4079
140.78740.24970.5240.31760.12361.0163-0.01410.00360.11270.06950.0964-0.0652-0.08170.0982-0.10320.29680.01670.05260.35180.00090.85919.47976.224712.6468
152.3527.58379.15668.34285.96483.15550.3346-0.60341.54230.1639-0.63390.08020.1696-0.24070.31810.36190.02290.06360.39560.00040.85115.48729.098811.9047
169.5521-5.59775.59256.9615-1.50638.21720.026-0.0317-0.6820.10630.77510.91360.5437-0.8989-0.76360.2393-0.06460.08570.36140.02680.7538-4.85818.592612.2185
170.98570.13330.64582.24881.65092.91170.0051-0.08380.01780.05090.034-0.0064-0.06660.0515-0.05520.26570.01770.04370.25580.07220.777512.723816.98344.9308
182.06081.35893.11821.9353.77466.2214-0.182-0.10520.5193-0.1544-0.19650.4392-0.2320.01020.49390.422-0.01270.08360.3284-0.04240.988214.718824.7492-0.8921
193.7552-0.88730.21512.0896-1.04743.51790.2156-0.05760.5751-0.0217-0.06760.212-0.1501-0.1345-0.13030.2783-0.01490.06820.2804-0.01140.72989.160617.931-0.0566
201.7083-0.2152-1.66230.97040.25185.788-0.01340.0222-0.3431-0.013-0.00890.0740.4212-0.2537-0.00310.36010.0133-0.0210.31580.04580.68231.1764-13.924163.5375
211.3795-0.5729-0.18983.1315-0.67465.09090.02720.05520.0475-0.1127-0.0386-0.10150.1215-0.0950.04630.3431-0.00360.02650.3218-0.03370.766323.7326-6.871864.1683
220.423-0.68431.2441.8865-3.08069.75010.11790.046-0.0728-0.11360.02040.22140.0115-0.3767-0.09520.3385-0.0081-0.00580.3184-0.07060.702223.12430.152749.5119
236.705-0.39822.8270.44830.56882.452-0.06270.08170.9273-0.0615-0.0360.4065-0.378-0.02050.20320.53180.05140.04740.367-0.00260.804939.174513.04953.6603
246.8483.328-1.27482.1023-8.19488.43750.04730.33660.1064-0.89790.1262-0.72630.09830.0421-0.19160.41640.05260.05290.4888-0.00390.592855.0153-1.005256.7812
257.8312.87725.90124.29310.17377.25540.48640.5068-0.92570.41160.075-0.37130.78580.1367-0.84910.49580.09090.07960.4451-0.05280.777740.6574-19.717456.2288
262.39824.6483-2.11179.1954-4.8512.5854-0.0557-0.2743-0.7403-0.9195-0.4506-1.0220.42750.44170.50230.58920.15920.02390.6983-0.09950.994952.5278-2.672648.94
271.5282-0.4015-0.65992.4214-0.83714.166-0.0034-0.09870.0309-0.0403-0.0140.0512-0.03030.17570.0220.20170.0042-0.02820.2923-0.00720.599437.3817-3.127567.6488
281.7425-0.56690.24498.6186-6.30592.7342-0.0536-0.04830.26850.2399-0.2435-0.806-0.37380.25350.26070.44090.0107-0.00120.3534-0.04580.730542.79946.79771.9621
291.6911-0.9021-1.18237.6904-0.81935.45560.12660.30640.6570.3336-0.23310.163-0.4927-0.19490.14810.35590.03950.05310.4070.03540.869749.20073.02564.8801
305.4031-0.56153.49561.5108-1.55539.83210.00430.01080.0062-0.0502-0.11180.00080.20250.32720.14460.3475-0.00250.01530.24130.01340.595641.5677-3.898471.0746
312.7135-0.01611.38711.2990.13084.83520.00170.04780.4108-0.1358-0.2728-0.0442-0.29170.1320.29210.38920.02670.09570.35090.01550.700514.733412.972562.7183
320.47970.41710.23741.1231.91475.5140.0204-0.02670.1816-0.0089-0.0250.017-0.0271-0.08880.04980.33290.04450.0460.3375-0.02820.737620.5663.999758.5174
334.3767-0.6543-2.69535.21251.57823.0918-0.1696-0.0019-0.42060.1875-0.13080.6520.3229-0.55920.28890.4034-0.0345-0.01760.60160.02930.9493-1.276-7.16456.2735
346.59611.9563-6.87916.0490.8892.33790.53591.09671.11530.17630.3950.7341-0.7344-1.3125-0.89120.37940.12640.00570.67350.03881.00673.303520.129657.8082
352.84291.7351.88092.50442.6734.1034-0.07340.08320.1656-0.2115-0.18520.4143-0.084-0.60380.30770.34270.02130.06250.50630.03380.83370.17033.726858.6879
364.90565.34392.07636.07953.32245.34010.1656-0.0969-0.6210.3939-0.0441-0.38480.5214-0.065-0.08760.30350.04810.04980.3660.0750.68258.0109-3.58174.6691
372.7939-0.143-0.31530.03950.65123.53730.17550.0560.05870.1118-0.20580.59560.1778-0.7002-0.05750.4777-0.00860.08740.5721-0.09371.0566-1.67580.979269.5848
383.0475-7.7597-5.77057.87574.59745.54660.3889-0.19090.3271-0.25620.1233-0.1469-0.4566-0.3018-0.58140.36070.00010.08220.5169-0.00190.75654.9618-0.321274.2393
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 3 through 26 )A3 - 26
2X-RAY DIFFRACTION2chain 'A' and (resid 27 through 50 )A27 - 50
3X-RAY DIFFRACTION3chain 'A' and (resid 51 through 80 )A51 - 80
4X-RAY DIFFRACTION4chain 'A' and (resid 81 through 141 )A81 - 141
5X-RAY DIFFRACTION5chain 'A' and (resid 142 through 171 )A142 - 171
6X-RAY DIFFRACTION6chain 'A' and (resid 172 through 191 )A172 - 191
7X-RAY DIFFRACTION7chain 'A' and (resid 192 through 213 )A192 - 213
8X-RAY DIFFRACTION8chain 'A' and (resid 214 through 233 )A214 - 233
9X-RAY DIFFRACTION9chain 'A' and (resid 234 through 283 )A234 - 283
10X-RAY DIFFRACTION10chain 'A' and (resid 284 through 318 )A284 - 318
11X-RAY DIFFRACTION11chain 'A' and (resid 319 through 348 )A319 - 348
12X-RAY DIFFRACTION12chain 'A' and (resid 349 through 391 )A349 - 391
13X-RAY DIFFRACTION13chain 'B' and (resid 3 through 65 )B3 - 65
14X-RAY DIFFRACTION14chain 'B' and (resid 66 through 171 )B66 - 171
15X-RAY DIFFRACTION15chain 'B' and (resid 172 through 191 )B172 - 191
16X-RAY DIFFRACTION16chain 'B' and (resid 192 through 213 )B192 - 213
17X-RAY DIFFRACTION17chain 'B' and (resid 214 through 302 )B214 - 302
18X-RAY DIFFRACTION18chain 'B' and (resid 303 through 329 )B303 - 329
19X-RAY DIFFRACTION19chain 'B' and (resid 330 through 391 )B330 - 391
20X-RAY DIFFRACTION20chain 'C' and (resid 3 through 55 )C3 - 55
21X-RAY DIFFRACTION21chain 'C' and (resid 56 through 104 )C56 - 104
22X-RAY DIFFRACTION22chain 'C' and (resid 105 through 141 )C105 - 141
23X-RAY DIFFRACTION23chain 'C' and (resid 142 through 171 )C142 - 171
24X-RAY DIFFRACTION24chain 'C' and (resid 172 through 191 )C172 - 191
25X-RAY DIFFRACTION25chain 'C' and (resid 192 through 213 )C192 - 213
26X-RAY DIFFRACTION26chain 'C' and (resid 214 through 233 )C214 - 233
27X-RAY DIFFRACTION27chain 'C' and (resid 234 through 283 )C234 - 283
28X-RAY DIFFRACTION28chain 'C' and (resid 284 through 318 )C284 - 318
29X-RAY DIFFRACTION29chain 'C' and (resid 319 through 348 )C319 - 348
30X-RAY DIFFRACTION30chain 'C' and (resid 349 through 391 )C349 - 391
31X-RAY DIFFRACTION31chain 'D' and (resid 3 through 65 )D3 - 65
32X-RAY DIFFRACTION32chain 'D' and (resid 66 through 141 )D66 - 141
33X-RAY DIFFRACTION33chain 'D' and (resid 142 through 191 )D142 - 191
34X-RAY DIFFRACTION34chain 'D' and (resid 192 through 213 )D192 - 213
35X-RAY DIFFRACTION35chain 'D' and (resid 214 through 265 )D214 - 265
36X-RAY DIFFRACTION36chain 'D' and (resid 266 through 302 )D266 - 302
37X-RAY DIFFRACTION37chain 'D' and (resid 303 through 367 )D303 - 367
38X-RAY DIFFRACTION38chain 'D' and (resid 368 through 391 )D368 - 391

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