National Health and Medical Research Council (NHMRC, Australia)
APP1164216
オーストラリア
引用
ジャーナル: Sci Adv / 年: 2021 タイトル: The structure of an infectious immature flavivirus redefines viral architecture and maturation. 著者: Natalee D Newton / Joshua M Hardy / Naphak Modhiran / Leon E Hugo / Alberto A Amarilla / Summa Bibby / Hariprasad Venugopal / Jessica J Harrison / Renee J Traves / Roy A Hall / Jody Hobson- ...著者: Natalee D Newton / Joshua M Hardy / Naphak Modhiran / Leon E Hugo / Alberto A Amarilla / Summa Bibby / Hariprasad Venugopal / Jessica J Harrison / Renee J Traves / Roy A Hall / Jody Hobson-Peters / Fasséli Coulibaly / Daniel Watterson / 要旨: Flaviviruses are the cause of severe human diseases transmitted by mosquitoes and ticks. These viruses use a potent fusion machinery to enter target cells that needs to be restrained during viral ...Flaviviruses are the cause of severe human diseases transmitted by mosquitoes and ticks. These viruses use a potent fusion machinery to enter target cells that needs to be restrained during viral assembly and egress. A molecular chaperone, premembrane (prM) maintains the virus particles in an immature, fusion-incompetent state until they exit the cell. Taking advantage of an insect virus that produces particles that are both immature and infectious, we determined the structure of the first immature flavivirus with a complete spike by cryo-electron microscopy. Unexpectedly, the prM chaperone forms a supporting pillar that maintains the immature spike in an asymmetric and upright state, primed for large rearrangements upon acidification. The collapse of the spike along a path defined by the prM chaperone is required, and its inhibition by a multivalent immunoglobulin M blocks infection. The revised architecture and collapse model are likely to be conserved across flaviviruses.
名称: Binjari virus / タイプ: VIRUS 詳細: M and E from Binjari virus form a complex that assembles into anti-parallel dimers, (M-E)2, in the T=3 icosahedral particle. Entity ID: all / 由来: MULTIPLE SOURCES
分子量
値: 22 MDa / 実験値: NO
由来(天然)
生物種: Binjari virus (ウイルス) / 株: BFTA20
由来(組換発現)
生物種: Aedes albopictus (ヒトスジシマカ) / 細胞: C6/36
ウイルスについての詳細
中空か: NO / エンベロープを持つか: YES / 単離: SPECIES / タイプ: VIRION
天然宿主
生物種: Ochlerotatus normanensis
ウイルス殻
直径: 470 nm / 三角数 (T数): 3
緩衝液
pH: 8
緩衝液成分
ID
濃度
名称
式
Buffer-ID
1
12mM
Tris
(HOCH2)3CNH2
1
2
120mM
sodiumchloride
NaCl
1
3
1mM
Ethylenediaminetetraaceticacid (EDTA)
C10H16N2O8
1
試料
濃度: 4 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES