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Open data
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Basic information
Entry | Database: PDB / ID: 7kka | ||||||
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Title | Fluoride channel Fluc-Ec2 mutant S81A with bromide | ||||||
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![]() | MEMBRANE PROTEIN / fluoride channel | ||||||
Function / homology | ![]() fluoride channel activity / cellular detoxification of fluoride / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | McIlwain, B.C. / Stockbridge, R.B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The fluoride permeation pathway and anion recognition in Fluc family fluoride channels. Authors: McIlwain, B.C. / Gundepudi, R. / Koff, B.B. / Stockbridge, R.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 101.7 KB | Display | ![]() |
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PDB format | ![]() | 76.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1012.1 KB | Display | ![]() |
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Full document | ![]() | 1015.6 KB | Display | |
Data in XML | ![]() | 17.6 KB | Display | |
Data in CIF | ![]() | 24.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7kk8C ![]() 7kk9C ![]() 7kkbC ![]() 7kkrC ![]() 5a43S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Antibody / Sugars , 3 types, 7 molecules ABCD![](data/chem/img/DMU.gif)
![](data/chem/img/DMU.gif)
#1: Protein | Mass: 13599.307 Da / Num. of mol.: 2 / Mutation: S81A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 10375.529 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #6: Sugar | |
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-Non-polymers , 4 types, 63 molecules ![](data/chem/img/NA.gif)
![](data/chem/img/BR.gif)
![](data/chem/img/F.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/BR.gif)
![](data/chem/img/F.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-NA / | ||||
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#4: Chemical | #5: Chemical | #7: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.66 Å3/Da / Density % sol: 78.28 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / Details: 0.1 glycine pH9 31% PEG 600 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Dec 6, 2017 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.5→39.08 Å / Num. obs: 36655 / % possible obs: 99.6 % / Redundancy: 13.7 % / CC1/2: 0.998 / Rmerge(I) obs: 0.146 / Rpim(I) all: 0.041 / Rrim(I) all: 0.152 / Net I/σ(I): 11.9 / Num. measured all: 502931 / Scaling rejects: 229 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5A43 Resolution: 2.5→39.08 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 30.46 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 162.72 Å2 / Biso mean: 66.7313 Å2 / Biso min: 40.63 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.5→39.08 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 13
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