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Open data
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Basic information
Entry | Database: PDB / ID: 5a43 | ||||||
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Title | Crystal structure of a dual topology fluoride ion channel. | ||||||
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![]() | TRANSPORT PROTEIN / FLUORIDE ION CHANNEL / FLUC / EC2 | ||||||
Function / homology | ![]() inorganic anion transmembrane transporter activity / plasma membrane => GO:0005886 Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Stockbridge, R.B. / Kolmakova-Partensky, L. / Shane, T. / Koide, A. / Koide, S. / Miller, C. / Newstead, S. | ||||||
![]() | ![]() Title: Crystal Structures of a Double-Barrelled Fluoride Ion Channel. Authors: Stockbridge, R.B. / Kolmakova-Partensky, L. / Shane, T. / Koide, A. / Koide, S. / Miller, C. / Newstead, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.9 KB | Display | ![]() |
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PDB format | ![]() | 78 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 575.6 KB | Display | ![]() |
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Full document | ![]() | 582.5 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 17.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5a40C ![]() 5nkqC ![]() 5a41 C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
-Protein , 2 types, 4 molecules ABCD
#1: Protein | Mass: 13863.006 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 10365.372 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 1 types, 3 molecules ![](data/chem/img/DMU.gif)
#4: Sugar |
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-Non-polymers , 3 types, 22 molecules ![](data/chem/img/F.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #5: Chemical | ChemComp-NA / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.03 Å3/Da / Density % sol: 79 % / Description: NONE |
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Crystal grow | pH: 6 / Details: 29% PEG550 MME, 50 MM LINO3, 100 MM ADA PH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 20, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→25 Å / Num. obs: 34593 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 14.3 % / Biso Wilson estimate: 62.65 Å2 / Rmerge(I) obs: 0.17 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 14.3 % / Rmerge(I) obs: 0.99 / Mean I/σ(I) obs: 1.9 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.58→24.246 Å / SU ML: 0.45 / σ(F): 1.36 / Phase error: 32.67 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.65 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.58→24.246 Å
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Refine LS restraints |
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LS refinement shell |
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