+
Open data
-
Basic information
Entry | Database: PDB / ID: 7kkb | ||||||
---|---|---|---|---|---|---|---|
Title | Fluoride channel Fluc-Ec2 mutant S81C with bromide | ||||||
![]() |
| ||||||
![]() | MEMBRANE PROTEIN / fluoride channel | ||||||
Function / homology | ![]() fluoride channel activity / cellular detoxification of fluoride / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | McIlwain, B.C. / Stockbridge, R.B. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: The fluoride permeation pathway and anion recognition in Fluc family fluoride channels. Authors: McIlwain, B.C. / Gundepudi, R. / Koff, B.B. / Stockbridge, R.B. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 188.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 148.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 998.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 998.7 KB | Display | |
Data in XML | ![]() | 16.8 KB | Display | |
Data in CIF | ![]() | 22.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7kk8C ![]() 7kk9C ![]() 7kkaC ![]() 7kkrC ![]() 5a43S S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
-Protein / Antibody / Sugars , 3 types, 7 molecules ABCD![](data/chem/img/DMU.gif)
![](data/chem/img/DMU.gif)
#1: Protein | Mass: 13599.307 Da / Num. of mol.: 2 / Mutation: S81C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 10375.529 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #6: Sugar | |
---|
-Non-polymers , 4 types, 7 molecules ![](data/chem/img/BR.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/F.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/F.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | ChemComp-NA / | #5: Chemical | #7: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 5.68 Å3/Da / Density % sol: 78.34 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion / Details: 0.1M glycine pH 9 36% PEG 600 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Feb 28, 2018 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.91836 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.9→46.65 Å / Num. obs: 23623 / % possible obs: 99.9 % / Redundancy: 12.5 % / CC1/2: 0.981 / Rmerge(I) obs: 0.37 / Rpim(I) all: 0.108 / Rrim(I) all: 0.386 / Net I/σ(I): 9.8 / Num. measured all: 295813 / Scaling rejects: 112 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
|
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 5A43 Resolution: 2.9→46.65 Å / SU ML: 0.4 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 29.18 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 211.3 Å2 / Biso mean: 68.6302 Å2 / Biso min: 33.55 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.9→46.65 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 8 / % reflection obs: 100 %
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: 1.527 Å / Origin y: -42.7316 Å / Origin z: -21.6967 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|