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Yorodumi- PDB-7kht: The acyl chains of phosphoinositide PIP3 alter the structure and ... -
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-Basic information
Entry | Database: PDB / ID: 7kht | |||||||||||||||
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Title | The acyl chains of phosphoinositide PIP3 alter the structure and function of nuclear receptor Steroidogenic Factor-1 (SF-1) | |||||||||||||||
Components |
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Keywords | LIPID BINDING PROTEIN / PI(3 / 4 / 5)P3 acyl chains / acyl chain length / acyl chain induced conformational change / nuclear PIP2 / nuclear PIP3 / nuclear receptor | |||||||||||||||
Function / homology | Function and homology information primary sex determination / response to gonadotropin-releasing hormone / Sertoli cell differentiation / negative regulation of female gonad development / positive regulation of mitochondrial DNA metabolic process / positive regulation of muscle tissue development / sex determination / regulation of steroid biosynthetic process / positive regulation of male gonad development / Transcriptional regulation of testis differentiation ...primary sex determination / response to gonadotropin-releasing hormone / Sertoli cell differentiation / negative regulation of female gonad development / positive regulation of mitochondrial DNA metabolic process / positive regulation of muscle tissue development / sex determination / regulation of steroid biosynthetic process / positive regulation of male gonad development / Transcriptional regulation of testis differentiation / luteinization / calcineurin-mediated signaling / positive regulation of cellular respiration / Transcriptional regulation of pluripotent stem cells / positive regulation of fatty acid oxidation / tissue development / Leydig cell differentiation / : / male sex determination / maintenance of protein location in nucleus / hormone metabolic process / : / lncRNA binding / cellular respiration / response to muscle activity / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / temperature homeostasis / adrenal gland development / female gonad development / positive regulation of ATP biosynthetic process / response to starvation / intracellular glucose homeostasis / fatty acid oxidation / response to dietary excess / adipose tissue development / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / energy homeostasis / brown fat cell differentiation / positive regulation of gluconeogenesis / digestion / respiratory electron transport chain / hormone-mediated signaling pathway / mitochondrion organization / SUMOylation of transcription cofactors / nuclear receptor coactivator activity / RNA splicing / gluconeogenesis / transcription coregulator binding / transcription initiation at RNA polymerase II promoter / nuclear receptor binding / transcription coregulator activity / negative regulation of smooth muscle cell proliferation / RNA polymerase II transcription regulatory region sequence-specific DNA binding / phospholipid binding / circadian regulation of gene expression / Heme signaling / SUMOylation of intracellular receptors / Transcriptional activation of mitochondrial biogenesis / regulation of circadian rhythm / PPARA activates gene expression / PML body / chromatin DNA binding / mRNA processing / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / male gonad development / Regulation of RUNX2 expression and activity / nuclear receptor activity / positive regulation of DNA-binding transcription factor activity / Circadian Clock / sequence-specific double-stranded DNA binding / cellular response to oxidative stress / positive regulation of cold-induced thermogenesis / protein-containing complex assembly / neuron apoptotic process / DNA-binding transcription factor binding / negative regulation of neuron apoptotic process / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription by RNA polymerase II / transcription coactivator activity / protein stabilization / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / ubiquitin protein ligase binding / chromatin binding / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / enzyme binding / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / zinc ion binding / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||||||||
Biological species | Homo sapiens (human) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.504 Å | |||||||||||||||
Authors | Blind, R.D. | |||||||||||||||
Funding support | United States, 4items
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Citation | Journal: J.Lipid Res. / Year: 2021 Title: The acyl chains of phosphoinositide PIP3 alter the structure and function of nuclear receptor steroidogenic factor-1. Authors: Bryant, J.M. / Malabanan, M.M. / Vanderloop, B.H. / Nichols, C.M. / Haratipour, Z. / Poon, K.T. / Sherrod, S.D. / McLean, J.A. / Blind, R.D. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7kht.cif.gz | 164.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7kht.ent.gz | 131.3 KB | Display | PDB format |
PDBx/mmJSON format | 7kht.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kh/7kht ftp://data.pdbj.org/pub/pdb/validation_reports/kh/7kht | HTTPS FTP |
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-Related structure data
Related structure data | 4qjrS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27903.406 Da / Num. of mol.: 1 / Mutation: C247S, C412S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NR5A1, AD4BP, FTZF1, SF1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q13285 |
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#2: Protein/peptide | Mass: 1523.854 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q9UBK2 |
#3: Chemical | ChemComp-WES / ( |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.65 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 20% PEG 3350 and 0.2 M magnesium formate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97857 Å |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Jun 20, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→38.54 Å / Num. obs: 21999 / % possible obs: 98.38 % / Redundancy: 2 % / Biso Wilson estimate: 70.45 Å2 / CC1/2: 1 / CC star: 1 / Net I/σ(I): 21.8 |
Reflection shell | Resolution: 2.504→2.594 Å / Redundancy: 2 % / Mean I/σ(I) obs: 2.6 / Num. unique obs: 1053 / CC1/2: 0.912 / CC star: 0.977 / % possible all: 98.78 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4QJR Resolution: 2.504→38.537 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 0.23 / Phase error: 32.41 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.504→38.537 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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