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- PDB-7kf4: Crystal structure from SARS-CoV-2 NendoU NSP15 -

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Basic information

Entry
Database: PDB / ID: 7kf4
TitleCrystal structure from SARS-CoV-2 NendoU NSP15
ComponentsUridylate-specific endoribonuclease
KeywordsVIRAL PROTEIN / HYDROLASE / NSP15 / NendoU / covid-19 / covid / sars / sars-cov-2 / endoribonuclease
Function / homology
Function and homology information


Maturation of replicase proteins / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Transcription of SARS-CoV-2 sgRNAs / host cell endosome / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Translation of Replicase and Assembly of the Replication Transcription Complex / 5'-3' RNA helicase activity / RNA phosphodiester bond hydrolysis, exonucleolytic / Lyases; Phosphorus-oxygen lyases / modulation by virus of host autophagy ...Maturation of replicase proteins / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Transcription of SARS-CoV-2 sgRNAs / host cell endosome / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Translation of Replicase and Assembly of the Replication Transcription Complex / 5'-3' RNA helicase activity / RNA phosphodiester bond hydrolysis, exonucleolytic / Lyases; Phosphorus-oxygen lyases / modulation by virus of host autophagy / mRNA methylation / double membrane vesicle viral factory outer membrane / suppression by virus of host translation / ISG15-specific protease activity / host cell Golgi apparatus / Replication of the SARS-CoV-2 genome / suppression by virus of host type I interferon production / host cell endoplasmic reticulum / SARS coronavirus main proteinase / induction by virus of catabolism of host mRNA / cytoplasmic viral factory / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / 3'-5'-exoribonuclease activity / G-quadruplex RNA binding / host cell endoplasmic reticulum-Golgi intermediate compartment / suppression by virus of host ISG15-protein conjugation / protein K48-linked deubiquitination / suppression by virus of host toll-like receptor signaling pathway / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / transcription, RNA-templated / suppression by virus of host NF-kappaB cascade / viral transcription / modulation by virus of host protein ubiquitination / protein K63-linked deubiquitination / methyltransferase cap1 / positive stranded viral RNA replication / protein autoprocessing / cysteine-type peptidase activity / viral genome replication / mRNA (nucleoside-2'-O-)-methyltransferase activity / suppression by virus of host TRAF activity / helicase activity / ubiquitinyl hydrolase 1 / DNA helicase / thiol-dependent deubiquitinase / DNA helicase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / host cell perinuclear region of cytoplasm / viral protein processing / RNA helicase / induction by virus of host autophagy / endonuclease activity / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed 5'-3' RNA polymerase activity / suppression by virus of host type I interferon-mediated signaling pathway / transcription, DNA-templated / host cell cytoplasm / protein dimerization activity / : / protein homodimerization activity / zinc ion binding / integral component of membrane / ATP binding / identical protein binding / cytosol
Similarity search - Function
RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Nonstructural protein 14, betacoronavirus / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. ...RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Nonstructural protein 14, betacoronavirus / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Viral (Superfamily 1) RNA helicase / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 13, 1B domain, coronavirus / RNA polymerase, N-terminal, coronavirus / Coronavirus proofreading exoribonuclease / Non-structural protein NSP16, coronavirus-like / Non-structural protein 14, coronavirus / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Non-structural protein NSP15, middle domain superfamily / Coronavirus RNA-dependent RNA polymerase, N-terminal / Coronavirus 2'-O-methyltransferase / Coronavirus replicase NSP15, N-terminal oligomerisation / Coronavirus replicase NSP15, middle domain / Coronaviridae zinc-binding (CV ZBD) domain profile. / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Sarbecovirus Nsp3c-N domain profile. / Betacoronavirus replicase NSP3, N-terminal / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Polyprotein cleavage domain PL2pro superfamily, coronavirus / Non-structural protein NSP1 superfamily, betacoronavirus / Non-structural protein 2, SARS-CoV-like / Betacoronavirus SUD-C domain / Non-structural protein NSP3, N-terminal, betacoronavirus / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / NendoU domain, nidovirus / Endoribonuclease EndoU-like / Carbamoyl-phosphate synthase subdomain signature 2. / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Betacoronavirus Nsp3c-M domain profile. / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Betacoronavirus replicase NSP1 / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / Betacoronavirus single-stranded poly(A) binding domain / Non-structural protein NSP1, betacoronavirus / Coronavirus (CoV) Nsp1 globular domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / Betacoronavirus Nsp3c-C domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein 6, betacoronavirus / Replicase polyprotein, nucleic acid-binding domain superfamily / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Betacoronavirus nucleic acid-binding (NAB) / Non-structural protein NSP3A domain-like superfamily / Coronavirus (CoV) ExoN/MTase coactivator domain profile. / Coronavirus Nsp9 single-stranded RNA (ssRNA)-binding domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp8 cofactor domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile. / Papain-like protease, N-terminal domain superfamily, coronavirus / Lipocalin signature. / Papain-like viral protease, palm and finger domains, coronavirus / Nonstructural protein 2, N-terminal domain, coronavirus / Coronavirus replicase NSP2, N-terminal / Coronavirus Nsp3d Ubl domain profile. / Coronavirus Nsp3a Ubl domain profile. / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / Non-structural protein 2, C-terminal domain, coronavirus / Coronavirus replicase NSP2, C-terminal / Coronavirus Nsp4 C-terminal (Nsp4C) domain profile. / NSP3, second ubiquitin-like (Ubl) domain, coronavirus / Papain-like protease, thumb domain superfamily, coronavirus / Coronavirus replicase NSP7 / Peptidase family C16 domain profile. / Non-structural protein NSP7, coronavirus / Coronavirus replicase NSP4, C-terminal / Coronavirus RNA synthesis protein NSP10 / Coronavirus replicase NSP8 / Coronavirus replicase NSP6 / Coronavirus papain-like peptidase / Non-structural protein NSP8, coronavirus-like / Coronavirus replicase NSP4, N-terminal / Coronavirus endopeptidase C30 / Coronavirus replicase NSP3, C-terminal / Coronavirus main protease (M-pro) domain profile. / RNA synthesis protein NSP10, coronavirus / Non-structural protein NSP4, C-terminal superfamily, coronavirus / Non-structural protein 6, coronavirus / Non-structural protein NSP4, C-terminal, coronavirus / Non-structural protein NSP9, coronavirus / RNA synthesis protein NSP10 superfamily, coronavirus / Coronavirus replicase NSP4, N-terminal / Peptidase C16, coronavirus / Non-structural protein NSP8 superfamily, coronavirus / Peptidase C30, coronavirus / Non-structural protein NSP9 superfamily, coronavirus
Similarity search - Domain/homology
CITRIC ACID / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.61 Å
AuthorsGodoy, A.S. / Nakamura, A.M. / Pereira, H.M. / Noske, G.D. / Gawriljuk, V.O. / Fernandes, R.S. / Oliveira, K.I.Z. / Oliva, G.
Funding support Brazil, 1items
OrganizationGrant numberCountry
Sao Paulo Research Foundation (FAPESP)2013/07600-3 Brazil
CitationJournal: To Be Published
Title: Crystal structure from SARS-COV2 NendoU NSP15
Authors: Godoy, A.S. / Oliva, G.
History
DepositionOct 13, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 2, 2020Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Uridylate-specific endoribonuclease
B: Uridylate-specific endoribonuclease
C: Uridylate-specific endoribonuclease
D: Uridylate-specific endoribonuclease
E: Uridylate-specific endoribonuclease
F: Uridylate-specific endoribonuclease
hetero molecules


Theoretical massNumber of molelcules
Total (without water)235,82812
Polymers234,6756
Non-polymers1,1536
Water7,728429
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: native gel electrophoresis
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area20690 Å2
ΔGint-104 kcal/mol
Surface area84910 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)85.125, 151.163, 199.242
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP22121

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Components

#1: Protein
Uridylate-specific endoribonuclease / NSP15 / NendoU


Mass: 39112.535 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Production host: Escherichia coli (E. coli)
References: UniProt: P0DTD1, Hydrolases; Acting on ester bonds
#2: Chemical
ChemComp-CIT / CITRIC ACID / Citric acid


Mass: 192.124 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C6H8O7
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 429 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.74 Å3/Da / Density % sol: 55.05 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 5 / Details: 0.1 M trisodium citrate pH 5, 14 % w/v PEG6000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: MAX IV / Beamline: BioMAX / Wavelength: 0.976254 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 13, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.976254 Å / Relative weight: 1
ReflectionResolution: 2.61→49.16 Å / Num. obs: 78886 / % possible obs: 99.73 % / Redundancy: 13.8 % / CC1/2: 0.998 / Net I/σ(I): 8.24
Reflection shellResolution: 2.61→2.703 Å / Num. unique obs: 7756 / CC1/2: 0.527

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Processing

Software
NameVersionClassification
BUSTER2.10.3refinement
PDB_EXTRACT3.25data extraction
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6x1b
Resolution: 2.61→49.16 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.915 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.717 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.712 / SU Rfree Blow DPI: 0.293 / SU Rfree Cruickshank DPI: 0.298
RfactorNum. reflection% reflectionSelection details
Rfree0.246 4004 5.09 %RANDOM
Rwork0.219 ---
obs0.22 78707 99.8 %-
Displacement parametersBiso max: 282.46 Å2 / Biso mean: 74.07 Å2 / Biso min: 25.11 Å2
Baniso -1Baniso -2Baniso -3
1--27.3512 Å20 Å20 Å2
2--17.6677 Å20 Å2
3---9.6835 Å2
Refine analyzeLuzzati coordinate error obs: 0.44 Å
Refinement stepCycle: final / Resolution: 2.61→49.16 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16447 0 78 429 16954
Biso mean--108.38 57.7 -
Num. residues----2088
Refine LS restraints
Refine-IDTypeNumberRestraint functionWeightDev ideal
X-RAY DIFFRACTIONt_dihedral_angle_d5856SINUSOIDAL2
X-RAY DIFFRACTIONt_trig_c_planes469HARMONIC2
X-RAY DIFFRACTIONt_gen_planes2412HARMONIC5
X-RAY DIFFRACTIONt_it16897HARMONIC20
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_chiral_improper_torsion2226SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact19009SEMIHARMONIC4
X-RAY DIFFRACTIONt_bond_d16897HARMONIC20.01
X-RAY DIFFRACTIONt_angle_deg22946HARMONIC21.11
X-RAY DIFFRACTIONt_omega_torsion3.3
X-RAY DIFFRACTIONt_other_torsion19.7
LS refinement shellResolution: 2.61→2.68 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.303 298 5.21 %
Rwork0.266 5423 -
all0.268 5721 -
obs--99.69 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.00070.49360.04570.00041.42350.00260.1213-0.1053-0.1407-0.0469-0.1399-0.07490.3616-0.09280.01860.3273-0.04880.01040.2980.01170.3666.819746.625835.4651
20.1308-0.16591.06751.70941.23412.1755-0.04250.04780.22620.05210.0261-0.0676-0.0712-0.18380.01640.27930.0096-0.02810.2412-0.01980.392715.944767.90426.9555
31.4538-1.1410.48171.0101-0.38590.79650.30990.31320.1801-0.3723-0.3235-0.2990.28880.20420.01360.59720.13370.17570.49340.16480.423737.368457.64768.4215
40.0416-0.612-1.48990.24710.77760.63220.04460.2520.1614-0.1344-0.0326-0.25050.10610.113-0.01190.43540.08060.0970.45420.08410.583342.336561.878816.2166
52.01761.93951.94342.21922.34172.2385-1.1757-0.633.1517-4.4371-1.99970.8413-5.4473-3.48263.17540.50710.07720.75491.0198-0.33741.071745.655461.470726.3093
60.58450.4495-0.60671.0639-2.130200.08140.0129-0.0558-0.0547-0.1445-0.00330.2322-0.05830.06310.36380.02940.05110.3621-0.0110.387836.684546.571255.3226
70.22020.60260.55242.1698-1.26782.0557-0.01810.00520.08260.1070.01310.1621-0.06750.07280.0050.36170.005-0.06470.2582-0.02440.437227.551467.939863.7281
81.31530.5401-0.48770.511-1.09190.28920.1029-0.19740.04880.3082-0.06320.14560.0985-0.0922-0.03970.5496-0.06230.02260.4364-0.04770.39176.470857.409680.6584
90.06461.124-0.23690.04010.28980.34980.0078-0.10640.05490.13340.0784-0.07620.0522-0.1231-0.08630.4247-0.02990.08190.4073-0.06790.5297-0.20262.560575.6244
101.99631.98221.99872.01311.95412.028-0.4983-0.03490.46831.0826-0.3542-1.2449-1.3725-0.22990.85260.64170.33370.06311.15030.20050.8712-2.61261.764263.49
110.0039-0.16760.4381.0814-0.5060-0.17730.08370.05420.01380.1431-0.0480.0393-0.06410.03420.6885-0.02580.03360.39140.04120.37423.555320.797337.0657
120.54841.0421-0.8840.01370.37641.5460.02630.0048-0.1481-0.0329-0.02420.06190.08090.0031-0.00220.7584-0.04740.01870.2232-0.00070.44671.989917.145725.6717
130.2376-0.34110.0430.7073-0.12870.35130.09110.0924-0.01040.034-0.0910.01040.2097-0.0253-0.00010.6081-0.1105-0.0690.4819-0.01390.3516-0.487740.61618.5154
149.0345-8.8894-5.87249.14517.28829.6051-0.21680.0557-0.7543-0.04790.1524-0.2024-0.007-0.44440.06430.71830.24560.11720.81630.03991.1155-7.79846.484422.3961
150.0104-0.0340.24230.3907-0.56810.5484-0.207-0.09020.0191-0.01380.13840.16430.155-0.03470.06860.66550.00310.04970.42180.00170.466420.084120.730453.6662
160.4551-0.7303-0.16980.0627-0.14761.6992-0.1274-0.0506-0.253-0.08360.125-0.108-0.1162-0.01150.00240.42670.01210.15580.28020.02810.405541.515717.161965.1775
170-0.8932-0.39252.25390.80860-0.1813-0.11390.00650.14660.1696-0.1172-0.0880.16840.01170.55790.1614-0.05540.5076-0.04730.35940.104639.071183.6929
180.4346-0.2938-0.90060.0010.41510.4198-0.0158-0.08430.1461-0.02970.011-0.13140.1187-0.12470.00480.48060.1463-0.00530.4759-0.05780.453948.723942.531680.4701
192.0679-3.38672.11582.0372-2.73392.11460.9532-1.55461.4-0.6349-0.824-2.18860.4084-3.5272-0.12921.21540.05510.08010.9758-0.24690.531349.444145.305869.5477
200-0.4432-0.45060.4834-0.02760.46130.0044-0.01110.1177-0.0405-0.0143-0.1079-0.1293-0.01790.00990.3648-0.02030.02620.40970.03680.41444.587751.561652.2398
210.3891-0.1125-0.79910.3546-0.76960.9757-0.0019-0.0395-0.0017-0.17570.023-0.03090.359-0.0985-0.02110.4343-0.1514-0.0140.35970.01060.3356-6.13232.258358.9963
220.08860.06721.35760.0106-0.4470.0097-0.0487-0.51320.4194-0.1751-0.0150.25460.2892-0.24180.06370.6812-0.16940.03020.49330.0140.5306-4.966220.95970.9219
231.8724-0.23260.20530.69510.34550.6671-0.0825-0.202-0.1496-0.0220.06170.00960.31630.20480.02080.505-0.01670.0670.46260.030.379911.259717.806583.5372
2400.6570.29580.05630.77390.00020.06-0.12260.0122-0.2504-0.01740.00440.2166-0.0488-0.04260.5325-0.07080.05880.5062-0.00090.48327.541112.453676.2867
250.40630.36340.30780.57240.14960.10620.10880.2008-0.2696-0.1616-0.1719-0.17870.0454-0.07730.06310.6845-0.07130.02930.51430.010.5317.14164.079475.831
261.99341.99732.00181.99742.00411.99130.8149-2.1481-0.84383.71860.8111-2.99834.74322.649-1.6260.88270.49440.05880.77160.62681.343910.99459.240165.8591
270.8066-0.5611-0.79880.1063-0.64841.1705-0.02940.11110.00320.1950.02040.00720.20440.05860.0090.33560.03720.06110.35590.06350.426237.373848.276938.3603
280.7146-1.2890.03590.60520.66470.06130.1095-0.0065-0.0161-0.2003-0.0731-0.11050.39020.0632-0.03640.50530.16770.14480.33760.10330.329152.715330.610130.6557
291.2717-1.20010.3470.096-0.15870.00720.24660.3227-0.229-0.1393-0.21730.04890.83220.1183-0.02921.10660.26410.15110.58660.04770.404236.055316.896211.8043
300.85550.06451.34060.2073-0.16950.3860.1318-0.6054-0.7099-0.2475-0.0521-0.25021.7887-0.2589-0.07971.2765-0.0202-0.07430.64-0.08350.625136.84274.613715.1956
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1{ B|0 - B|60 }B0 - 60
2X-RAY DIFFRACTION2{ B|61 - B|183 }B61 - 183
3X-RAY DIFFRACTION3{ B|184 - B|267 }B184 - 267
4X-RAY DIFFRACTION4{ B|268 - B|345 }B268 - 345
5X-RAY DIFFRACTION5{ B|346 - B|346 }B346
6X-RAY DIFFRACTION6{ A|0 - A|60 }A0 - 60
7X-RAY DIFFRACTION7{ A|61 - A|183 }A61 - 183
8X-RAY DIFFRACTION8{ A|184 - A|276 }A184 - 276
9X-RAY DIFFRACTION9{ A|277 - A|345 }A277 - 345
10X-RAY DIFFRACTION10{ A|346 - A|346 }A346
11X-RAY DIFFRACTION11{ C|0 - C|60 }C0 - 60
12X-RAY DIFFRACTION12{ C|61 - C|183 }C61 - 183
13X-RAY DIFFRACTION13{ C|184 - C|345 }C184 - 345
14X-RAY DIFFRACTION14{ C|346 - C|346 }C346
15X-RAY DIFFRACTION15{ D|0 - D|60 }D0 - 60
16X-RAY DIFFRACTION16{ D|61 - D|183 }D61 - 183
17X-RAY DIFFRACTION17{ D|184 - D|276 }D184 - 276
18X-RAY DIFFRACTION18{ D|277 - D|345 }D277 - 345
19X-RAY DIFFRACTION19{ D|346 - D|346 }D346
20X-RAY DIFFRACTION20{ E|0 - E|38 }E0 - 38
21X-RAY DIFFRACTION21{ E|39 - E|183 }E39 - 183
22X-RAY DIFFRACTION22{ E|184 - E|203 }E184 - 203
23X-RAY DIFFRACTION23{ E|204 - E|276 }E204 - 276
24X-RAY DIFFRACTION24{ E|277 - E|325 }E277 - 325
25X-RAY DIFFRACTION25{ E|326 - E|345 }E326 - 345
26X-RAY DIFFRACTION26{ E|346 - E|346 }E346
27X-RAY DIFFRACTION27{ F|0 - F|60 }F0 - 60
28X-RAY DIFFRACTION28{ F|61 - F|183 }F61 - 183
29X-RAY DIFFRACTION29{ F|184 - F|325 }F184 - 325
30X-RAY DIFFRACTION30{ F|326 - F|345 }F326 - 345

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